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The map label for this gene is pth [H]

Identifier: 158338610

GI number: 158338610

Start: 5562672

End: 5563292

Strand: Direct

Name: pth [H]

Synonym: AM1_5514

Alternate gene names: 158338610

Gene position: 5562672-5563292 (Clockwise)

Preceding gene: 158338609

Following gene: 158338611

Centisome position: 85.53

GC content: 48.63

Gene sequence:

>621_bases
ATGACCGCTCCACTGATTATTCCTGAACTGATTGTCGGTTTAGGAAACCCTGGTTCCAAATATGATCGCACTCGCCATAA
TATTGGTTTTGCATTTATCGATCGATTGGCTCAGCACTGGCAAATTTCCTTAACTTCAGAACGGCGATTCCAAGGTGAAT
ACGGGGAAGGCTTGGGTGGCCAGGGTCGCCGGGTGCGCTTGCTTAAACCCCATACTTATATGAATAACTCTGGCCAATCC
ATTCGCGCGGTGATTGATTGGTTCAAGCTAGAGCCCACCTCTGTTTTAGTCATCTATGATGAAATGGCTTTGCCCGTTGG
TAAGTTGCGACTGCGGCTGAAGGGATCTGCCGGGGGACACAATGGGATGCGCTCCATCATTTCCCATTTGGGTACACCTG
AAGTACCCAGGCTACGAATTGGTATTGGCAGTCCACCCCCAGAAAGAGGCAAAGATACCATCTCTTTTGTGTTAGGACGT
TTTGCCCCGTTAGAGAACCAACTGCTTCCGGCGGTTCTTGATCTCGGTGTAGATGCGGTGGAGATGGCACTCAAGAAAGG
GATTGAGCAATCCATGACTCTTTTCAATGGGCGCACAGTTTCCATATCTAGTTCTGAATAA

Upstream 100 bases:

>100_bases
CCCCAAGCCTCAAGAACAGCCTCAGCTGGAAGGCCAAGAGATGGCTGAAGCCCCAACCGACGACGTAATGGCTGAATCTC
CTGAAGTAACGAACTCCTAA

Downstream 100 bases:

>100_bases
ACTCAATAATCCTGAGGCGAGATTATATAAGTTGAGCCTATCGATAGGAAATTCTAATCTATTTCTAGATGACAATGACC
TATGAGTCTTTCTCCGTAAG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTAPLIIPELIVGLGNPGSKYDRTRHNIGFAFIDRLAQHWQISLTSERRFQGEYGEGLGGQGRRVRLLKPHTYMNNSGQS
IRAVIDWFKLEPTSVLVIYDEMALPVGKLRLRLKGSAGGHNGMRSIISHLGTPEVPRLRIGIGSPPPERGKDTISFVLGR
FAPLENQLLPAVLDLGVDAVEMALKKGIEQSMTLFNGRTVSISSSE

Sequences:

>Translated_206_residues
MTAPLIIPELIVGLGNPGSKYDRTRHNIGFAFIDRLAQHWQISLTSERRFQGEYGEGLGGQGRRVRLLKPHTYMNNSGQS
IRAVIDWFKLEPTSVLVIYDEMALPVGKLRLRLKGSAGGHNGMRSIISHLGTPEVPRLRIGIGSPPPERGKDTISFVLGR
FAPLENQLLPAVLDLGVDAVEMALKKGIEQSMTLFNGRTVSISSSE
>Mature_205_residues
TAPLIIPELIVGLGNPGSKYDRTRHNIGFAFIDRLAQHWQISLTSERRFQGEYGEGLGGQGRRVRLLKPHTYMNNSGQSI
RAVIDWFKLEPTSVLVIYDEMALPVGKLRLRLKGSAGGHNGMRSIISHLGTPEVPRLRIGIGSPPPERGKDTISFVLGRF
APLENQLLPAVLDLGVDAVEMALKKGIEQSMTLFNGRTVSISSSE

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:
GO:0004045: Peptidyl-tRNA hydrolase
GO:0005737: Peptidyl-tRNA hydrolase
GO:0006412: Peptidyl-tRNA hydrolase
GO:0016787: Peptidyl-tRNA hydrolase

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=174, Percent_Identity=39.6551724137931, Blast_Score=111, Evalue=5e-25,
Organism=Escherichia coli, GI1787455, Length=184, Percent_Identity=40.7608695652174, Blast_Score=117, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321983, Length=175, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 22640; Mature: 22508

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLIIPELIVGLGNPGSKYDRTRHNIGFAFIDRLAQHWQISLTSERRFQGEYGEGLGG
CCCCHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCC
QGRRVRLLKPHTYMNNSGQSIRAVIDWFKLEPTSVLVIYDEMALPVGKLRLRLKGSAGGH
CCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHCCHHHEEEEEECCCCCC
NGMRSIISHLGTPEVPRLRIGIGSPPPERGKDTISFVLGRFAPLENQLLPAVLDLGVDAV
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHH
EMALKKGIEQSMTLFNGRTVSISSSE
HHHHHHCHHHHHHHHCCEEEEEECCC
>Mature Secondary Structure 
TAPLIIPELIVGLGNPGSKYDRTRHNIGFAFIDRLAQHWQISLTSERRFQGEYGEGLGG
CCCHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCC
QGRRVRLLKPHTYMNNSGQSIRAVIDWFKLEPTSVLVIYDEMALPVGKLRLRLKGSAGGH
CCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHCCHHHEEEEEECCCCCC
NGMRSIISHLGTPEVPRLRIGIGSPPPERGKDTISFVLGRFAPLENQLLPAVLDLGVDAV
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHH
EMALKKGIEQSMTLFNGRTVSISSSE
HHHHHHCHHHHHHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA