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The map label for this gene is rpoD [H]

Identifier: 158338579

GI number: 158338579

Start: 5539650

End: 5540456

Strand: Direct

Name: rpoD [H]

Synonym: AM1_5482

Alternate gene names: 158338579

Gene position: 5539650-5540456 (Clockwise)

Preceding gene: 158338576

Following gene: 158338581

Centisome position: 85.18

GC content: 48.95

Gene sequence:

>807_bases
TTGGCGACTCAACATGACAGTCTGCATTACTATCTCAAAGAGATAGGGCGGTTTCCGCTGCTGACCCATGAGGAAGAAAT
TACCCTAGCTCGGCAAGTCCAGGCAGGTAACCCTCGCGCAAAAAGAAAAATGATCGAGGCGAATCTCCGATTGGTGGTCT
CGATTGCCAAAAAACATCAGAATCGAGGTTTGCCGCTACTCGATTTGATTCAAGAAGGTAACTTGGGCTTAAGTATTGCC
GTTGATAAATTTGAGCCGGAGCAAGGGAATCGGTTTAGTACCTATGCCTACTGGTGGATTTTGCAGGGGGTGACCCGTTC
CTTGCAAAACAAAGGTCGCGTCGTCCGGTTGCCTGTGAAACATTGGCAAGTAGGTAATCAGATCAAACGCACTCGACATC
AGTTGAGCCAAGAGCTAGGTCGAGAACCCACCGTCGTTGAAATAGCTGACGCCATGGAGATGGATCTGACCCGCTTGAAA
AAGTACCTCCATAGCTTGCAAGAGACGGTGTCTTTAGACAAGTTTGTGGGCAGTGAGCAGGATACAACCCTAGGGGATCT
GATTGAGGGGAATGATTCCACGCAGTCCTACCTCGATCTGCTGCTGCAGGATGAGGAATTGTCTGGGTACTTGGCGTTGT
TGGATGATCGCCAGCGATTTGTGATTTCTGAGCGATATGGGTTGGAAGATGGTAAACCCAAGTCTATGGAGAAAATCGGC
CAAAAGTTGGGGATTAGCCGTGAACGGGTCCGGCAGATTATCAAAAAGGCGATGCAAACCCTAGAGAAATGCGCATTATC
GGCTTAG

Upstream 100 bases:

>100_bases
TGGATAAAAACCCTGGTTTTTTAAGAAATGCGAAGTTATCTTCCTATTGATCTGTTTTTGGGGTACTACCTCTACAACGG
CTATTTGCGGGGAAAAGAAA

Downstream 100 bases:

>100_bases
ATTAAAAAAAGGGCATGGAAGACCCATACCCTGATTGGTCTCTGTTGTGAAAATTAGGCTTCTGCGTCTACCTCTACTGC
CGTAACGGAATTGGCAGAAA

Product: RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MATQHDSLHYYLKEIGRFPLLTHEEEITLARQVQAGNPRAKRKMIEANLRLVVSIAKKHQNRGLPLLDLIQEGNLGLSIA
VDKFEPEQGNRFSTYAYWWILQGVTRSLQNKGRVVRLPVKHWQVGNQIKRTRHQLSQELGREPTVVEIADAMEMDLTRLK
KYLHSLQETVSLDKFVGSEQDTTLGDLIEGNDSTQSYLDLLLQDEELSGYLALLDDRQRFVISERYGLEDGKPKSMEKIG
QKLGISRERVRQIIKKAMQTLEKCALSA

Sequences:

>Translated_268_residues
MATQHDSLHYYLKEIGRFPLLTHEEEITLARQVQAGNPRAKRKMIEANLRLVVSIAKKHQNRGLPLLDLIQEGNLGLSIA
VDKFEPEQGNRFSTYAYWWILQGVTRSLQNKGRVVRLPVKHWQVGNQIKRTRHQLSQELGREPTVVEIADAMEMDLTRLK
KYLHSLQETVSLDKFVGSEQDTTLGDLIEGNDSTQSYLDLLLQDEELSGYLALLDDRQRFVISERYGLEDGKPKSMEKIG
QKLGISRERVRQIIKKAMQTLEKCALSA
>Mature_267_residues
ATQHDSLHYYLKEIGRFPLLTHEEEITLARQVQAGNPRAKRKMIEANLRLVVSIAKKHQNRGLPLLDLIQEGNLGLSIAV
DKFEPEQGNRFSTYAYWWILQGVTRSLQNKGRVVRLPVKHWQVGNQIKRTRHQLSQELGREPTVVEIADAMEMDLTRLKK
YLHSLQETVSLDKFVGSEQDTTLGDLIEGNDSTQSYLDLLLQDEELSGYLALLDDRQRFVISERYGLEDGKPKSMEKIGQ
KLGISRERVRQIIKKAMQTLEKCALSA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=269, Percent_Identity=40.1486988847584, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1789448, Length=235, Percent_Identity=42.5531914893617, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1789871, Length=282, Percent_Identity=28.0141843971631, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30736; Mature: 30605

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQHDSLHYYLKEIGRFPLLTHEEEITLARQVQAGNPRAKRKMIEANLRLVVSIAKKHQ
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
NRGLPLLDLIQEGNLGLSIAVDKFEPEQGNRFSTYAYWWILQGVTRSLQNKGRVVRLPVK
CCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHH
HWQVGNQIKRTRHQLSQELGREPTVVEIADAMEMDLTRLKKYLHSLQETVSLDKFVGSEQ
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DTTLGDLIEGNDSTQSYLDLLLQDEELSGYLALLDDRQRFVISERYGLEDGKPKSMEKIG
CCCHHHHHCCCCCHHHHHHHHHCCCHHCCHHEEECCCHHHHHHHHCCCCCCCCHHHHHHH
QKLGISRERVRQIIKKAMQTLEKCALSA
HHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATQHDSLHYYLKEIGRFPLLTHEEEITLARQVQAGNPRAKRKMIEANLRLVVSIAKKHQ
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
NRGLPLLDLIQEGNLGLSIAVDKFEPEQGNRFSTYAYWWILQGVTRSLQNKGRVVRLPVK
CCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHH
HWQVGNQIKRTRHQLSQELGREPTVVEIADAMEMDLTRLKKYLHSLQETVSLDKFVGSEQ
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DTTLGDLIEGNDSTQSYLDLLLQDEELSGYLALLDDRQRFVISERYGLEDGKPKSMEKIG
CCCHHHHHCCCCCHHHHHHHHHCCCHHCCHHEEECCCHHHHHHHHCCCCCCCCHHHHHHH
QKLGISRERVRQIIKKAMQTLEKCALSA
HHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]