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The map label for this gene is cca [H]

Identifier: 158338560

GI number: 158338560

Start: 5520139

End: 5521467

Strand: Reverse

Name: cca [H]

Synonym: AM1_5461

Alternate gene names: 158338560

Gene position: 5521467-5520139 (Counterclockwise)

Preceding gene: 158338561

Following gene: 158338558

Centisome position: 84.9

GC content: 52.67

Gene sequence:

>1329_bases
ATGTCTATCCAATCTGTCCTTGCTCCCCAAACTTGGCCCTTCCCAGCTAGCGATTTACCCCAATCTGCCTGCTTAGTGGG
GGGATGCGTGCGGGATGCCTTACTAAATCGCCATACGGCTTATTTAGATCTGGATTTTGTCTTGCCTAAAGCTGCAGTGG
AAACAGCTCGTAGGGTTTCCGAAAGTTGTAATGCCGGGTTTGTGCTTCTGGATGCCGAACGAGATATAGCGCGGGTGGTT
TTCCCAGACGCAACGGCAGATTTCGCCTTGCAGGTGGGGGATTCCTTAGAAGAGGACCTGCAGCGACGAGACTATACGAT
AAATGCGATCGCATATCATCCCTTTTCCGATACAGTGGTTGATCCGTTGATGGGGCAGCAAGATCTCGACGCAGGTGTGC
TACGCATGATCGCCGCCAAAAACCTAGAAGAAGATCCCTTGCGACTGTTGCGGGCCTATCGGCAAGCCGCCCAATTGGGG
TTTACCATTGAGCCCCAGACCCAAGCCAATATTCGTCAACTCACCCCTCAGTTAAAGGGCATTGCCGCAGAGCGGATCAA
AGTGGAGCTGAGCTATCTGTTGAGCGATTCCAAGGCCACACCTTGGGTTCATCAACTTTGGCAGGATGGCCTATTAAGCA
CATCGTTTCCCCATGCATCATCAACGAGCCTTGCTTTTTTACCCGCCATCGATCAAGCGGCTAGTCTGCTCCAGAAAGAG
GTCCCGGACTTAGCAACCGCTTTTCATCAGGAACTGAATGAGCGAGCCCAAGGAAAAGAGGCCGCCCAGCGCACCCTCCT
GTCCACCACTAAGTGGGTCTCCCTCCTGTCTGACAATCCAACTCAGGCAGAAATAACTCTAAAACAGATGAAGTTCAGTC
GGGGCGAAATCCATGTGATTACGCGGGTGTTGAACGGTTGGCCTCGCTTAAAACAGCTCTTGCAGCAGGAACACTGCCGC
CGTGTGGATCAGTTTTATCTCTTTCAAGACGCGGATACGACGTTCCCTGCTGTTGTGATGGTGACATTAGCGGTGGAGCT
GATGCAGCAATCTCTACGGGGGGCTGAGACGCCGTCTTGTCTACCAGCGCTAAAGCCTTGGATTGCAGAATATCTCAACC
CGCTCAGCGCTTTAGCTCATCCTCAACCCCTCTTATCAGGTTCAGCCTTAATGAAGTCCCTTCACTTATCCCCTGGGCCA
GAAGTGGGTCACCTGCTTTCACAATTGACTCTAGCCCAAGCGGAAGGCAGTATCACCACCTCAGAAGAGGCGTTGGCATT
GGCCCAAACCCTAGTCAGTTCGACGCCTTCAACGGCCGAGAACGGTTAG

Upstream 100 bases:

>100_bases
GTCTCTCCCTGCCCATTCCAACTTGATTGTCTGTTTGCCAGTGACTTGCCTGAGCATTTAGCTGTGGTAGGGCTTTTGCA
GGCTTTTTTCTTGATTTTGC

Downstream 100 bases:

>100_bases
TCGCAACCCAGACAACGATACTGCCCAGCACAACAGAGAATAGGCTTAACCCCATCACACCGATGATGGCGAAGCCTTGA
CCATAGCCGTATCGGCTCAC

Product: polyA polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSIQSVLAPQTWPFPASDLPQSACLVGGCVRDALLNRHTAYLDLDFVLPKAAVETARRVSESCNAGFVLLDAERDIARVV
FPDATADFALQVGDSLEEDLQRRDYTINAIAYHPFSDTVVDPLMGQQDLDAGVLRMIAAKNLEEDPLRLLRAYRQAAQLG
FTIEPQTQANIRQLTPQLKGIAAERIKVELSYLLSDSKATPWVHQLWQDGLLSTSFPHASSTSLAFLPAIDQAASLLQKE
VPDLATAFHQELNERAQGKEAAQRTLLSTTKWVSLLSDNPTQAEITLKQMKFSRGEIHVITRVLNGWPRLKQLLQQEHCR
RVDQFYLFQDADTTFPAVVMVTLAVELMQQSLRGAETPSCLPALKPWIAEYLNPLSALAHPQPLLSGSALMKSLHLSPGP
EVGHLLSQLTLAQAEGSITTSEEALALAQTLVSSTPSTAENG

Sequences:

>Translated_442_residues
MSIQSVLAPQTWPFPASDLPQSACLVGGCVRDALLNRHTAYLDLDFVLPKAAVETARRVSESCNAGFVLLDAERDIARVV
FPDATADFALQVGDSLEEDLQRRDYTINAIAYHPFSDTVVDPLMGQQDLDAGVLRMIAAKNLEEDPLRLLRAYRQAAQLG
FTIEPQTQANIRQLTPQLKGIAAERIKVELSYLLSDSKATPWVHQLWQDGLLSTSFPHASSTSLAFLPAIDQAASLLQKE
VPDLATAFHQELNERAQGKEAAQRTLLSTTKWVSLLSDNPTQAEITLKQMKFSRGEIHVITRVLNGWPRLKQLLQQEHCR
RVDQFYLFQDADTTFPAVVMVTLAVELMQQSLRGAETPSCLPALKPWIAEYLNPLSALAHPQPLLSGSALMKSLHLSPGP
EVGHLLSQLTLAQAEGSITTSEEALALAQTLVSSTPSTAENG
>Mature_441_residues
SIQSVLAPQTWPFPASDLPQSACLVGGCVRDALLNRHTAYLDLDFVLPKAAVETARRVSESCNAGFVLLDAERDIARVVF
PDATADFALQVGDSLEEDLQRRDYTINAIAYHPFSDTVVDPLMGQQDLDAGVLRMIAAKNLEEDPLRLLRAYRQAAQLGF
TIEPQTQANIRQLTPQLKGIAAERIKVELSYLLSDSKATPWVHQLWQDGLLSTSFPHASSTSLAFLPAIDQAASLLQKEV
PDLATAFHQELNERAQGKEAAQRTLLSTTKWVSLLSDNPTQAEITLKQMKFSRGEIHVITRVLNGWPRLKQLLQQEHCRR
VDQFYLFQDADTTFPAVVMVTLAVELMQQSLRGAETPSCLPALKPWIAEYLNPLSALAHPQPLLSGSALMKSLHLSPGPE
VGHLLSQLTLAQAEGSITTSEEALALAQTLVSSTPSTAENG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=223, Percent_Identity=32.7354260089686, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789436, Length=216, Percent_Identity=31.4814814814815, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48474; Mature: 48343

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQSVLAPQTWPFPASDLPQSACLVGGCVRDALLNRHTAYLDLDFVLPKAAVETARRVS
CCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEEHHHCCHHHHHHHHHHH
ESCNAGFVLLDAERDIARVVFPDATADFALQVGDSLEEDLQRRDYTINAIAYHPFSDTVV
HCCCCCEEEEECCCCEEEEECCCCCHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCHHHH
DPLMGQQDLDAGVLRMIAAKNLEEDPLRLLRAYRQAAQLGFTIEPQTQANIRQLTPQLKG
HHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHH
IAAERIKVELSYLLSDSKATPWVHQLWQDGLLSTSFPHASSTSLAFLPAIDQAASLLQKE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
VPDLATAFHQELNERAQGKEAAQRTLLSTTKWVSLLSDNPTQAEITLKQMKFSRGEIHVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHHHHCCCCHHHHH
TRVLNGWPRLKQLLQQEHCRRVDQFYLFQDADTTFPAVVMVTLAVELMQQSLRGAETPSC
HHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
LPALKPWIAEYLNPLSALAHPQPLLSGSALMKSLHLSPGPEVGHLLSQLTLAQAEGSITT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
SEEALALAQTLVSSTPSTAENG
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SIQSVLAPQTWPFPASDLPQSACLVGGCVRDALLNRHTAYLDLDFVLPKAAVETARRVS
CCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEEHHHCCHHHHHHHHHHH
ESCNAGFVLLDAERDIARVVFPDATADFALQVGDSLEEDLQRRDYTINAIAYHPFSDTVV
HCCCCCEEEEECCCCEEEEECCCCCHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCHHHH
DPLMGQQDLDAGVLRMIAAKNLEEDPLRLLRAYRQAAQLGFTIEPQTQANIRQLTPQLKG
HHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHH
IAAERIKVELSYLLSDSKATPWVHQLWQDGLLSTSFPHASSTSLAFLPAIDQAASLLQKE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
VPDLATAFHQELNERAQGKEAAQRTLLSTTKWVSLLSDNPTQAEITLKQMKFSRGEIHVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHHHHCCCCHHHHH
TRVLNGWPRLKQLLQQEHCRRVDQFYLFQDADTTFPAVVMVTLAVELMQQSLRGAETPSC
HHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
LPALKPWIAEYLNPLSALAHPQPLLSGSALMKSLHLSPGPEVGHLLSQLTLAQAEGSITT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
SEEALALAQTLVSSTPSTAENG
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11442348 [H]