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The map label for this gene is dnaB [H]

Identifier: 158338449

GI number: 158338449

Start: 5407829

End: 5409190

Strand: Reverse

Name: dnaB [H]

Synonym: AM1_5349

Alternate gene names: 158338449

Gene position: 5409190-5407829 (Counterclockwise)

Preceding gene: 158338450

Following gene: 158338446

Centisome position: 83.17

GC content: 50.81

Gene sequence:

>1362_bases
ATGGTTTACGAGCTAGATTTCCAACCCGCTAGCGATCGCTTACCCCCTCAGAATGTTGAAGCCGAGGAATCCATTCTGGG
GGGTATTCTGCTGGACCCAGAAGCAATTGGCCGAGTTGCAGATTTGCTTCGGCCTGAGACCTTTTATCTCAGCGCTCATC
AAGAAATTTACCGCGCCGCACTGAATCTGCATGGGCAAGGCAGTCCCACTGACTTGATGTGTGTCACTGCTTGGCTACAG
GATCATGAACTCCTAGATAAAGTCGGTGGGCAAGGTAAGCTAGCCCAGCTAGTGGACCGTACGGTCAGCGCTATCAATAT
CGATCAATATGCCGCCCTGATTAAAGACAAGTTTCTGCGGCGACAGCTGATCAAAGGGGCGACGGAGGTCATGCAATTGG
CCTACGACACCTCCAAGACCTTAGAAACGGTCTTGGACGAAGCAGAACAGCAAATTTTTAACGTCACGCAAGATCGGGTG
CGGCAAGGCCTAGTCGCCACAGAAGATATTTTGGCGAGTACCTTCGCTGATTTAGAACAGCGATCTTTAGGGCAGGTTTT
ACCCGGTTTATCCTGTAATTTCTACGACCTGGATGCCATGACCCAAGGCTTCCAGCGGTCGGACTTAATTATTGTGGCGG
GTCGTCCTGCCATGGGTAAAACGGCGTTAGCCCTACAAATTGCTCGGCAAATTTGTGAAATCCATAACCTGGGGGTTGCC
ATCTTTAGCTTGGAGATGTCCAAAGAGCAGCTCGTCCAGCGGATGTTGGCTGGAGAAGCGGGGATTGATAGTAACTACCT
GCGGGCTGGACGCATCAATCAGACCCAGTGGGAACCCCTAAGCCGAGCCATAGGTAAACTCTCTCAGCTTTCAATTTATA
TTGATGATGCGCCTAACCCCAGCATTACCGAGTTGAGATCGAATGCTCGGCGGCTGCAGTCAGAACATCCTGAAGGCTTA
GGTATGATTCTTTTAGATTATCTCCAGCTGATGGGTGGAGGTGATGCCTCGGATGGCCGGGTGCAAGAAATTTCTAAGAT
TACTCGGGCACTGAAAGGACTGGCTCGGGAACTGAATGTGCCCGTGGTTGCTCTCTCTCAGTTGAGCCGGGGTGTGGAGT
CTCGGACTAATAAGCGACCGTTAATGTCGGATCTGCGGGAATCAGGTTCTATTGAGCAGGATGCGGATTTGATTATGCTG
CTCTATCGGGATGACTACTATGATCCTGATAGCCCTGAGCGAGGTATCTGCGAACTGAATATTGCCAAGCACCGGAATGG
CCCGGTAGGGACAGTCAAACTGTTGTTTGAGCCGAAACTGACCCGCTTCTTCAATTTGGCTAAACCCGAAGGTATGGGTT
AA

Upstream 100 bases:

>100_bases
ACCGTCAAAGTGGAAGTTGTTCCTGAATAAATGCTAGGGTGCTGGCACCTCCCGAGTAAGATTATCTTCTAACTAAAGGG
ATTTACCTGTTGGCATACCT

Downstream 100 bases:

>100_bases
TGCAGTAAAAAGCCCCCAGTAGTCGATAACTGAGGGCTTGTTGAATCTTTACTGGGTAGAGTTGGGACTACTCTTTAGAG
CCGCCTAGCTGATTGCTAAT

Product: replicative DNA helicase

Products: NA

Alternate protein names: Asp dnaB intein [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MVYELDFQPASDRLPPQNVEAEESILGGILLDPEAIGRVADLLRPETFYLSAHQEIYRAALNLHGQGSPTDLMCVTAWLQ
DHELLDKVGGQGKLAQLVDRTVSAINIDQYAALIKDKFLRRQLIKGATEVMQLAYDTSKTLETVLDEAEQQIFNVTQDRV
RQGLVATEDILASTFADLEQRSLGQVLPGLSCNFYDLDAMTQGFQRSDLIIVAGRPAMGKTALALQIARQICEIHNLGVA
IFSLEMSKEQLVQRMLAGEAGIDSNYLRAGRINQTQWEPLSRAIGKLSQLSIYIDDAPNPSITELRSNARRLQSEHPEGL
GMILLDYLQLMGGGDASDGRVQEISKITRALKGLARELNVPVVALSQLSRGVESRTNKRPLMSDLRESGSIEQDADLIML
LYRDDYYDPDSPERGICELNIAKHRNGPVGTVKLLFEPKLTRFFNLAKPEGMG

Sequences:

>Translated_453_residues
MVYELDFQPASDRLPPQNVEAEESILGGILLDPEAIGRVADLLRPETFYLSAHQEIYRAALNLHGQGSPTDLMCVTAWLQ
DHELLDKVGGQGKLAQLVDRTVSAINIDQYAALIKDKFLRRQLIKGATEVMQLAYDTSKTLETVLDEAEQQIFNVTQDRV
RQGLVATEDILASTFADLEQRSLGQVLPGLSCNFYDLDAMTQGFQRSDLIIVAGRPAMGKTALALQIARQICEIHNLGVA
IFSLEMSKEQLVQRMLAGEAGIDSNYLRAGRINQTQWEPLSRAIGKLSQLSIYIDDAPNPSITELRSNARRLQSEHPEGL
GMILLDYLQLMGGGDASDGRVQEISKITRALKGLARELNVPVVALSQLSRGVESRTNKRPLMSDLRESGSIEQDADLIML
LYRDDYYDPDSPERGICELNIAKHRNGPVGTVKLLFEPKLTRFFNLAKPEGMG
>Mature_453_residues
MVYELDFQPASDRLPPQNVEAEESILGGILLDPEAIGRVADLLRPETFYLSAHQEIYRAALNLHGQGSPTDLMCVTAWLQ
DHELLDKVGGQGKLAQLVDRTVSAINIDQYAALIKDKFLRRQLIKGATEVMQLAYDTSKTLETVLDEAEQQIFNVTQDRV
RQGLVATEDILASTFADLEQRSLGQVLPGLSCNFYDLDAMTQGFQRSDLIIVAGRPAMGKTALALQIARQICEIHNLGVA
IFSLEMSKEQLVQRMLAGEAGIDSNYLRAGRINQTQWEPLSRAIGKLSQLSIYIDDAPNPSITELRSNARRLQSEHPEGL
GMILLDYLQLMGGGDASDGRVQEISKITRALKGLARELNVPVVALSQLSRGVESRTNKRPLMSDLRESGSIEQDADLIML
LYRDDYYDPDSPERGICELNIAKHRNGPVGTVKLLFEPKLTRFFNLAKPEGMG

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:
GO:0003677: Replicative DNA helicase
GO:0003678: Replicative DNA helicase
GO:0004386: Replicative DNA helicase
GO:0005524: Replicative DNA helicase
GO:0006260: Replicative DNA helicase

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=453, Percent_Identity=43.2671081677704, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50219; Mature: 50219

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYELDFQPASDRLPPQNVEAEESILGGILLDPEAIGRVADLLRPETFYLSAHQEIYRAA
CEEEECCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCCCEEEHHHHHHHHHH
LNLHGQGSPTDLMCVTAWLQDHELLDKVGGQGKLAQLVDRTVSAINIDQYAALIKDKFLR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RQLIKGATEVMQLAYDTSKTLETVLDEAEQQIFNVTQDRVRQGLVATEDILASTFADLEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RSLGQVLPGLSCNFYDLDAMTQGFQRSDLIIVAGRPAMGKTALALQIARQICEIHNLGVA
HHHHHHCCCCCCCCEEHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEE
IFSLEMSKEQLVQRMLAGEAGIDSNYLRAGRINQTQWEPLSRAIGKLSQLSIYIDDAPNP
EEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHEEEEEECCCCC
SITELRSNARRLQSEHPEGLGMILLDYLQLMGGGDASDGRVQEISKITRALKGLARELNV
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PVVALSQLSRGVESRTNKRPLMSDLRESGSIEQDADLIMLLYRDDYYDPDSPERGICELN
CHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEE
IAKHRNGPVGTVKLLFEPKLTRFFNLAKPEGMG
EHHCCCCCCEEEEEEECHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVYELDFQPASDRLPPQNVEAEESILGGILLDPEAIGRVADLLRPETFYLSAHQEIYRAA
CEEEECCCCCCCCCCCCCCCHHHHHHCCEEECHHHHHHHHHHHCCCCEEEHHHHHHHHHH
LNLHGQGSPTDLMCVTAWLQDHELLDKVGGQGKLAQLVDRTVSAINIDQYAALIKDKFLR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RQLIKGATEVMQLAYDTSKTLETVLDEAEQQIFNVTQDRVRQGLVATEDILASTFADLEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RSLGQVLPGLSCNFYDLDAMTQGFQRSDLIIVAGRPAMGKTALALQIARQICEIHNLGVA
HHHHHHCCCCCCCCEEHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEE
IFSLEMSKEQLVQRMLAGEAGIDSNYLRAGRINQTQWEPLSRAIGKLSQLSIYIDDAPNP
EEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHEEEEEECCCCC
SITELRSNARRLQSEHPEGLGMILLDYLQLMGGGDASDGRVQEISKITRALKGLARELNV
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PVVALSQLSRGVESRTNKRPLMSDLRESGSIEQDADLIMLLYRDDYYDPDSPERGICELN
CHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEE
IAKHRNGPVGTVKLLFEPKLTRFFNLAKPEGMG
EHHCCCCCCEEEEEEECHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]