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The map label for this gene is ykuT [H]

Identifier: 158338415

GI number: 158338415

Start: 5380015

End: 5381604

Strand: Reverse

Name: ykuT [H]

Synonym: AM1_5315

Alternate gene names: 158338415

Gene position: 5381604-5380015 (Counterclockwise)

Preceding gene: 158338416

Following gene: 158338414

Centisome position: 82.75

GC content: 50.82

Gene sequence:

>1590_bases
ATGCCTGCCATCCGCCGTCGACTATTTCTTGCTTTAGTTAGCGTTACGCTAACGATTCTTTTGCATATCCCGGTCTGGGC
TCAAGCCCCCAGTCCCGTTGCCAACCCCTCAACCCAGCAGCCGCTAATTATTCCGAACCTGTCAAACCCTCTGGTTTCGG
ATTGGATTTGGTTGGATGGTCGCCGTCTCTTCAAAATCGCGGCCACTAAAGGCAATCTAGCTGAGCGTATTCAAGCGGTC
CAGGACAGTCTGGAGACCAGCAAACGTAAGTATCTCCGCTCAGATTCAGTCGACCCCAAGGTAGACATTCGGATCAGCAA
TAACTTACCCGTCATTTATGTTGATGAGCAGCATTTAATGACCATCACCTCCCTGGATGCAGAACTGCGAGGGCTGGACG
TCACCACCCTAGATGATCAAATTAAATCTATTTTAGAAGTGGCCCTGCGCCGCGCAAGGACCGAGCGCCAGCCCCAGTTC
TTGCGACAGCAGGGGATTCTTACGGCCGCCGTGATTGGCTGTGCCGTTCTTTTAAATCGCCTCGTCCATTTTTGGCAACG
TCGGTTAAGACGCCCTGCTAAAGCAGTCGCCGCCCCTAAAACAGATGGATCGCTGCAGCTGCCGCCGGTCCAATTTTGGG
TAGGATTCCTCGGGGTACTCCGGCAAGTTCTCAGTATTCTGCAGATCGCAATAGGGGTAATCTGCCTGCTCATCTGCTTG
GACCGATTTCCCTACACCCGTCCTATCAATATTTGGTTGCTCAAGAGCCTACCCATACCCCTCACCTTAACCGGGGTCGG
ATTAGTTACCTATCTGTTGATCCGATTGAGTTGGGCCTTAGTCAACCGCTTTGCCTTAGCCTTAACCGTCACTCCCTTTC
TCTCCCCCATGGCATCGCGGCGGATGCTGCTGAGAATTTCCACCATTGCCAATGTAATCAAAGGAATTGCCGTTCCGATT
TGGATTAGCTTAGGCATCATTGTGGCCTTAATCCTGCTTAAAGTGGATGTCGGTCCCTTACTAGCGGGTGTCGGTTTGGT
GGGGGTCGCCCTTTCTCTTGCCTCCCAAAGCGTTATCAAAGATGCCCTCAATGGGTTCCTAGTGATTGTGGAAGATCAGT
TTGGGGTAGGCGATGTAATCACAGTCGATCAGTACACCGGCGTAGTTGAAACTTTAAACTTGCGCATGACCCAAATTCGC
AATGCTGAAGGTCAGTTAATCACCATCCCCAATAGTGAAATTCGCATCGTGGCCAACCTCTCCAGCGAATGGTCACGGGT
CGATCTCAATATTCCCATTCCCTACCATACTGATATTGACCAAATGCTAGATCTGATTCAGTCCACGGCCCAGACTATGG
CAGATGAACCGGATTGGCAAAAACAGATTATAGAACCACCGCAACTGATGGGTGTGGATGATTTTGATCATCGGGGGCCT
GTCGTCAAGGTCTGGATCAAAACACAGCCGCTCCAACAATGGGCCGTGTCTAGAGAATTGCGTCGTCGGTTAAAGTTAGC
CTTCGACGAAGCCAATATTTCCATCCCCGTCCCTCAGCAGCAGTTATGGCTATATCGATCTCATCCTTGA

Upstream 100 bases:

>100_bases
ACTCAAGCCAACTCGGGCAACGGTTGCCACCTAGTACCAATCCTAGAGAAGGTGGCATTGGCAGCCAGTGTAGAACATTG
AGACACCATCCAAACCGTGA

Downstream 100 bases:

>100_bases
AGCCATCTGTACAGACTAGAATTGTTGGCCCCCTTTTAAGCATGAATATCGCAGAACGATGGCATCCAATCTTAGGAGGC
TGACTCCCAAACTATTACTG

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MPAIRRRLFLALVSVTLTILLHIPVWAQAPSPVANPSTQQPLIIPNLSNPLVSDWIWLDGRRLFKIAATKGNLAERIQAV
QDSLETSKRKYLRSDSVDPKVDIRISNNLPVIYVDEQHLMTITSLDAELRGLDVTTLDDQIKSILEVALRRARTERQPQF
LRQQGILTAAVIGCAVLLNRLVHFWQRRLRRPAKAVAAPKTDGSLQLPPVQFWVGFLGVLRQVLSILQIAIGVICLLICL
DRFPYTRPINIWLLKSLPIPLTLTGVGLVTYLLIRLSWALVNRFALALTVTPFLSPMASRRMLLRISTIANVIKGIAVPI
WISLGIIVALILLKVDVGPLLAGVGLVGVALSLASQSVIKDALNGFLVIVEDQFGVGDVITVDQYTGVVETLNLRMTQIR
NAEGQLITIPNSEIRIVANLSSEWSRVDLNIPIPYHTDIDQMLDLIQSTAQTMADEPDWQKQIIEPPQLMGVDDFDHRGP
VVKVWIKTQPLQQWAVSRELRRRLKLAFDEANISIPVPQQQLWLYRSHP

Sequences:

>Translated_529_residues
MPAIRRRLFLALVSVTLTILLHIPVWAQAPSPVANPSTQQPLIIPNLSNPLVSDWIWLDGRRLFKIAATKGNLAERIQAV
QDSLETSKRKYLRSDSVDPKVDIRISNNLPVIYVDEQHLMTITSLDAELRGLDVTTLDDQIKSILEVALRRARTERQPQF
LRQQGILTAAVIGCAVLLNRLVHFWQRRLRRPAKAVAAPKTDGSLQLPPVQFWVGFLGVLRQVLSILQIAIGVICLLICL
DRFPYTRPINIWLLKSLPIPLTLTGVGLVTYLLIRLSWALVNRFALALTVTPFLSPMASRRMLLRISTIANVIKGIAVPI
WISLGIIVALILLKVDVGPLLAGVGLVGVALSLASQSVIKDALNGFLVIVEDQFGVGDVITVDQYTGVVETLNLRMTQIR
NAEGQLITIPNSEIRIVANLSSEWSRVDLNIPIPYHTDIDQMLDLIQSTAQTMADEPDWQKQIIEPPQLMGVDDFDHRGP
VVKVWIKTQPLQQWAVSRELRRRLKLAFDEANISIPVPQQQLWLYRSHP
>Mature_528_residues
PAIRRRLFLALVSVTLTILLHIPVWAQAPSPVANPSTQQPLIIPNLSNPLVSDWIWLDGRRLFKIAATKGNLAERIQAVQ
DSLETSKRKYLRSDSVDPKVDIRISNNLPVIYVDEQHLMTITSLDAELRGLDVTTLDDQIKSILEVALRRARTERQPQFL
RQQGILTAAVIGCAVLLNRLVHFWQRRLRRPAKAVAAPKTDGSLQLPPVQFWVGFLGVLRQVLSILQIAIGVICLLICLD
RFPYTRPINIWLLKSLPIPLTLTGVGLVTYLLIRLSWALVNRFALALTVTPFLSPMASRRMLLRISTIANVIKGIAVPIW
ISLGIIVALILLKVDVGPLLAGVGLVGVALSLASQSVIKDALNGFLVIVEDQFGVGDVITVDQYTGVVETLNLRMTQIRN
AEGQLITIPNSEIRIVANLSSEWSRVDLNIPIPYHTDIDQMLDLIQSTAQTMADEPDWQKQIIEPPQLMGVDDFDHRGPV
VKVWIKTQPLQQWAVSRELRRRLKLAFDEANISIPVPQQQLWLYRSHP

Specific function: May play a role in resistance to osmotic downshock [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI87081787, Length=218, Percent_Identity=27.9816513761468, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789291, Length=289, Percent_Identity=26.9896193771626, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 59235; Mature: 59104

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIRRRLFLALVSVTLTILLHIPVWAQAPSPVANPSTQQPLIIPNLSNPLVSDWIWLDG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHCEEEECC
RRLFKIAATKGNLAERIQAVQDSLETSKRKYLRSDSVDPKVDIRISNNLPVIYVDEQHLM
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCEE
TITSLDAELRGLDVTTLDDQIKSILEVALRRARTERQPQFLRQQGILTAAVIGCAVLLNR
EEECCCHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHH
LVHFWQRRLRRPAKAVAAPKTDGSLQLPPVQFWVGFLGVLRQVLSILQIAIGVICLLICL
HHHHHHHHHCCCHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRFPYTRPINIWLLKSLPIPLTLTGVGLVTYLLIRLSWALVNRFALALTVTPFLSPMASR
HCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLLRISTIANVIKGIAVPIWISLGIIVALILLKVDVGPLLAGVGLVGVALSLASQSVIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DALNGFLVIVEDQFGVGDVITVDQYTGVVETLNLRMTQIRNAEGQLITIPNSEIRIVANL
HHHCCEEEEEECCCCCCCEEEEHHHHCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEC
SSEWSRVDLNIPIPYHTDIDQMLDLIQSTAQTMADEPDWQKQIIEPPQLMGVDDFDHRGP
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHCCCCCCCCCCC
VVKVWIKTQPLQQWAVSRELRRRLKLAFDEANISIPVPQQQLWLYRSHP
EEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHEEEECCC
>Mature Secondary Structure 
PAIRRRLFLALVSVTLTILLHIPVWAQAPSPVANPSTQQPLIIPNLSNPLVSDWIWLDG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHCEEEECC
RRLFKIAATKGNLAERIQAVQDSLETSKRKYLRSDSVDPKVDIRISNNLPVIYVDEQHLM
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCEE
TITSLDAELRGLDVTTLDDQIKSILEVALRRARTERQPQFLRQQGILTAAVIGCAVLLNR
EEECCCHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHH
LVHFWQRRLRRPAKAVAAPKTDGSLQLPPVQFWVGFLGVLRQVLSILQIAIGVICLLICL
HHHHHHHHHCCCHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRFPYTRPINIWLLKSLPIPLTLTGVGLVTYLLIRLSWALVNRFALALTVTPFLSPMASR
HCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLLRISTIANVIKGIAVPIWISLGIIVALILLKVDVGPLLAGVGLVGVALSLASQSVIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DALNGFLVIVEDQFGVGDVITVDQYTGVVETLNLRMTQIRNAEGQLITIPNSEIRIVANL
HHHCCEEEEEECCCCCCCEEEEHHHHCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEC
SSEWSRVDLNIPIPYHTDIDQMLDLIQSTAQTMADEPDWQKQIIEPPQLMGVDDFDHRGP
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHCCCCCCCCCCC
VVKVWIKTQPLQQWAVSRELRRRLKLAFDEANISIPVPQQQLWLYRSHP
EEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]