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The map label for this gene is rumA [C]

Identifier: 158338369

GI number: 158338369

Start: 5335247

End: 5336683

Strand: Reverse

Name: rumA [C]

Synonym: AM1_5265

Alternate gene names: 158338369

Gene position: 5336683-5335247 (Counterclockwise)

Preceding gene: 158338371

Following gene: 158338368

Centisome position: 82.06

GC content: 49.13

Gene sequence:

>1437_bases
ATGCCAGTGGATAACCTTCAAAATGCCCAGGAACAACAACGACCAGAAAGCCATTGGCAACAAGGTAGCCTAGTGGATCT
AGAGATTACAGATCTCAGTCCGACTGGAGATGGGGTGGGTCGATGGGGAAACCAAAATCTGGTTGTTTTTGTCCCCAACA
CCGTACCTGGCGATCACATCACTGTTCGGTTGATTCGGGTTAAACCCAGATATGCCCACGGTCAGCTTCAAAGTATAAAG
CAGGCCTCACCTCACCGAGTGCGGCCGCAATGCATTGTGGCTGATAAGTGTGGGGGGTGTCAGTGGCAGCAGGTAGACTA
CGCCTATCAGTTGGTTGCCAAGCAGAATCATGTGATTCAAGCCCTAGAGCGAATCGGTAAATTCCAGTCCCCTGTTGTCG
AGCCAGTGCTGGCTGCTCCATCTCCGATTGAGTACCGCAATAAGGTAACCTATCCCATTGTGTTAAGCCCCAACACCAAC
CGAGTCCAGGCAGGGTATTACCGCAAAGGGAGCCACAAGCTCATTAATCTCAATCAATGCCCCGTTCAAGATCCTCACTT
TAATTCGTTTTTAGCGGAAATTAAAGAAGATATTCAAAAGCAAGATTGGCCCATCTATAACGAACAAAATCATCGGGGTC
TGATTCGCCACCTCTCGATCCGCATCGGTCGACGGACGGGAGAAATTCTAATGACGTTGATTATTAATGCCAAACCGAAC
CAATCTTTATCTGAATTAGTCGATCTAGATGCCCAAGTACAGGAGTGGCTGGAGCGCTATCCCCGACTAGTGGGAATTAG
TCTGAACTTCAATCCCCATCGAACCAATCGTATCTTTGGCCATGACTCCACTTGCATTGCCGGTCACCACTATTTGCAAG
AAACCTTTGCGGGGTTAACCTTTCAGCTCCGTCATGATACCTTTTTCCAGGTGCATACTGAACAAGCGGAAGTTTTGCTC
AATACCCTTTTCGATCACCTTCAGCTTATGGGCAGCGAACAGGTTGTTGATCTGTACTGCGGGATTGGTACTCTTACGCT
TCCCCTAGCTCAGCGAGTTAAAGAGATTATTGGGATTGAATTGCAGGCCGCAGCCGTAGACCAAGCCCAGACCAATGCCA
CCCTTAATCAAATTAGCAACGTAACGTTTGCTGCTGGGACGGCCAAAGCGTGCTTAAGGGAGCTGCCTTTTGTTCCGGAT
CTGGTGCTGTTAGATCCGCCTCGTAAAGGCTGCCACCAAAGTGTGATTGAACAACTCCTGGCTCTCAAGCCTGCGCAAAT
CGTCTATGTCAGTTGCCATTCCGCGACCCTTGCTCGGGATTTGAATTTGCTGTGTCAATCAGGACAATATGACTTGACAC
GAGTCCAACCCGTCGACTTTTTTCCTCAAACGTCCCATGTGGAATGTGTCGCGTTCCTCAATCGGCGGGCTTCATGA

Upstream 100 bases:

>100_bases
CCTATATAGCTATGGCAGTGTTAATCAGCCTTGGCCCATTTAGAGCCTCAGCTTAAGACAGCCGCCGTCTTATTTATCCC
AATTGTTAGTGCATCAATTT

Downstream 100 bases:

>100_bases
TTGAGACCGTTGTTGACATTGACTGTTTAACCTTTCTACTAATCAGCATTTTTATTCTTGGGATTGGCATCCTTCTGACC
CGCACCCTCCCTCTCCTCAG

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MPVDNLQNAQEQQRPESHWQQGSLVDLEITDLSPTGDGVGRWGNQNLVVFVPNTVPGDHITVRLIRVKPRYAHGQLQSIK
QASPHRVRPQCIVADKCGGCQWQQVDYAYQLVAKQNHVIQALERIGKFQSPVVEPVLAAPSPIEYRNKVTYPIVLSPNTN
RVQAGYYRKGSHKLINLNQCPVQDPHFNSFLAEIKEDIQKQDWPIYNEQNHRGLIRHLSIRIGRRTGEILMTLIINAKPN
QSLSELVDLDAQVQEWLERYPRLVGISLNFNPHRTNRIFGHDSTCIAGHHYLQETFAGLTFQLRHDTFFQVHTEQAEVLL
NTLFDHLQLMGSEQVVDLYCGIGTLTLPLAQRVKEIIGIELQAAAVDQAQTNATLNQISNVTFAAGTAKACLRELPFVPD
LVLLDPPRKGCHQSVIEQLLALKPAQIVYVSCHSATLARDLNLLCQSGQYDLTRVQPVDFFPQTSHVECVAFLNRRAS

Sequences:

>Translated_478_residues
MPVDNLQNAQEQQRPESHWQQGSLVDLEITDLSPTGDGVGRWGNQNLVVFVPNTVPGDHITVRLIRVKPRYAHGQLQSIK
QASPHRVRPQCIVADKCGGCQWQQVDYAYQLVAKQNHVIQALERIGKFQSPVVEPVLAAPSPIEYRNKVTYPIVLSPNTN
RVQAGYYRKGSHKLINLNQCPVQDPHFNSFLAEIKEDIQKQDWPIYNEQNHRGLIRHLSIRIGRRTGEILMTLIINAKPN
QSLSELVDLDAQVQEWLERYPRLVGISLNFNPHRTNRIFGHDSTCIAGHHYLQETFAGLTFQLRHDTFFQVHTEQAEVLL
NTLFDHLQLMGSEQVVDLYCGIGTLTLPLAQRVKEIIGIELQAAAVDQAQTNATLNQISNVTFAAGTAKACLRELPFVPD
LVLLDPPRKGCHQSVIEQLLALKPAQIVYVSCHSATLARDLNLLCQSGQYDLTRVQPVDFFPQTSHVECVAFLNRRAS
>Mature_477_residues
PVDNLQNAQEQQRPESHWQQGSLVDLEITDLSPTGDGVGRWGNQNLVVFVPNTVPGDHITVRLIRVKPRYAHGQLQSIKQ
ASPHRVRPQCIVADKCGGCQWQQVDYAYQLVAKQNHVIQALERIGKFQSPVVEPVLAAPSPIEYRNKVTYPIVLSPNTNR
VQAGYYRKGSHKLINLNQCPVQDPHFNSFLAEIKEDIQKQDWPIYNEQNHRGLIRHLSIRIGRRTGEILMTLIINAKPNQ
SLSELVDLDAQVQEWLERYPRLVGISLNFNPHRTNRIFGHDSTCIAGHHYLQETFAGLTFQLRHDTFFQVHTEQAEVLLN
TLFDHLQLMGSEQVVDLYCGIGTLTLPLAQRVKEIIGIELQAAAVDQAQTNATLNQISNVTFAAGTAKACLRELPFVPDL
VLLDPPRKGCHQSVIEQLLALKPAQIVYVSCHSATLARDLNLLCQSGQYDLTRVQPVDFFPQTSHVECVAFLNRRAS

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=381, Percent_Identity=29.3963254593176, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI51173878, Length=381, Percent_Identity=29.3963254593176, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI269784680, Length=375, Percent_Identity=26.9333333333333, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI269784676, Length=375, Percent_Identity=26.9333333333333, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI20149688, Length=375, Percent_Identity=26.9333333333333, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI269784678, Length=360, Percent_Identity=27.2222222222222, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1789148, Length=457, Percent_Identity=29.3216630196937, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1787083, Length=392, Percent_Identity=26.0204081632653, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790403, Length=157, Percent_Identity=28.6624203821656, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71997438, Length=165, Percent_Identity=30.9090909090909, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322909, Length=450, Percent_Identity=23.3333333333333, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24666579, Length=387, Percent_Identity=28.1653746770026, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 53884; Mature: 53753

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVDNLQNAQEQQRPESHWQQGSLVDLEITDLSPTGDGVGRWGNQNLVVFVPNTVPGDHI
CCCCCCCCHHHHCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCE
TVRLIRVKPRYAHGQLQSIKQASPHRVRPQCIVADKCGGCQWQQVDYAYQLVAKQNHVIQ
EEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
ALERIGKFQSPVVEPVLAAPSPIEYRNKVTYPIVLSPNTNRVQAGYYRKGSHKLINLNQC
HHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEECCEEECCCCCEEECCCC
PVQDPHFNSFLAEIKEDIQKQDWPIYNEQNHRGLIRHLSIRIGRRTGEILMTLIINAKPN
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHEEEEEEEECCCC
QSLSELVDLDAQVQEWLERYPRLVGISLNFNPHRTNRIFGHDSTCIAGHHYLQETFAGLT
CHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCEEHHHHHHHHHHCCCE
FQLRHDTFFQVHTEQAEVLLNTLFDHLQLMGSEQVVDLYCGIGTLTLPLAQRVKEIIGIE
EEEECCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHCCE
LQAAAVDQAQTNATLNQISNVTFAAGTAKACLRELPFVPDLVLLDPPRKGCHQSVIEQLL
EEHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHH
ALKPAQIVYVSCHSATLARDLNLLCQSGQYDLTRVQPVDFFPQTSHVECVAFLNRRAS
HCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
PVDNLQNAQEQQRPESHWQQGSLVDLEITDLSPTGDGVGRWGNQNLVVFVPNTVPGDHI
CCCCCCCHHHHCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCE
TVRLIRVKPRYAHGQLQSIKQASPHRVRPQCIVADKCGGCQWQQVDYAYQLVAKQNHVIQ
EEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
ALERIGKFQSPVVEPVLAAPSPIEYRNKVTYPIVLSPNTNRVQAGYYRKGSHKLINLNQC
HHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEECCEEECCCCCEEECCCC
PVQDPHFNSFLAEIKEDIQKQDWPIYNEQNHRGLIRHLSIRIGRRTGEILMTLIINAKPN
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHEEEEEEEECCCC
QSLSELVDLDAQVQEWLERYPRLVGISLNFNPHRTNRIFGHDSTCIAGHHYLQETFAGLT
CHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCEEHHHHHHHHHHCCCE
FQLRHDTFFQVHTEQAEVLLNTLFDHLQLMGSEQVVDLYCGIGTLTLPLAQRVKEIIGIE
EEEECCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHCCE
LQAAAVDQAQTNATLNQISNVTFAAGTAKACLRELPFVPDLVLLDPPRKGCHQSVIEQLL
EEHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHH
ALKPAQIVYVSCHSATLARDLNLLCQSGQYDLTRVQPVDFFPQTSHVECVAFLNRRAS
HCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]