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The map label for this gene is xth [H]

Identifier: 158338364

GI number: 158338364

Start: 5329779

End: 5330585

Strand: Direct

Name: xth [H]

Synonym: AM1_5260

Alternate gene names: 158338364

Gene position: 5329779-5330585 (Clockwise)

Preceding gene: 158338362

Following gene: 158338370

Centisome position: 81.95

GC content: 44.49

Gene sequence:

>807_bases
ATGAAAATTGCTAGCTGGAATGTAAATTCTATCCGAACACGCTTGGAACATGTTTTGGATTGGTTATATACCCAATCGAT
AGATGTTTTATGTCTGCAGGAAACGAAGGTTATTGATGAAGACTTCCCGAAAGCCTCTTTTGAAGAAGAAGGTTACCAGG
TCTATGTTTCAGGGCAAAAAGCTTATAACGGCGTGGCTTTAATCAGTCGGACGACTCTTTCTGAGGTCAGTTCTGGGTTC
ACCCCCTTACTGGGTCCAGACTTGACCCAGCACTATGACCAACAAAAACGGCTGATCACGGGGATCATTTCACCAGGGGT
TCGCATTTTAAATCTTTATGTTCCCAATGGCTCGGAAATAGACAGTGATAAATATGTCTACAAATTAGAGTGGTTGGAGC
TATTACATCACTATGTTGAGAAGTTGCTATCTCAAGATCCCCAGCATCTTCTCATCTGTGGTGATTTTAATATTGCTCTA
GACGATCGAGATATGCATGATCCGTCCAAGCGGGAGGCCCATATTATGTCTACAGATCGGGAGCGACAGGCTTTGCAAAA
GGTTCTGGCGCTTGGATTAGAAGATGTCTTCCGAAAGTTCAACTCTAAGTCCGAGCAGTTTAGCTGGTGGAATTATCGGG
CAGGTTCTTTTCAACGGAATAAGGGTTGGCGGATTGACCATCACTATTTAACGCCAACCTTATATGAACAGGCAACGGCT
TGCACAATTGACGCAGCCCCTCGTCGGTTACCCAAACCGAGCGATCATGCTCCCGTTGTGGTTGAGATCGATACTCAAAA
CTTATAG

Upstream 100 bases:

>100_bases
AAACTTATCGTCAATTTGGCTACTAATTCTCATAAGTTTGTGCGAAAACAAGAAAAAAAATTGTTAAGGATTTTGTTTCA
GGAATAGAAAAGAAAACGTG

Downstream 100 bases:

>100_bases
GTAAGAAAACTAAGAAACCTCTGGCGCCATGGAACAACGGGGGCAAGGCAACATAATTTGTAGCCAGGCTCCGCCAGTTT
GGGGATGGTTGCGGGCCCGA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKIASWNVNSIRTRLEHVLDWLYTQSIDVLCLQETKVIDEDFPKASFEEEGYQVYVSGQKAYNGVALISRTTLSEVSSGF
TPLLGPDLTQHYDQQKRLITGIISPGVRILNLYVPNGSEIDSDKYVYKLEWLELLHHYVEKLLSQDPQHLLICGDFNIAL
DDRDMHDPSKREAHIMSTDRERQALQKVLALGLEDVFRKFNSKSEQFSWWNYRAGSFQRNKGWRIDHHYLTPTLYEQATA
CTIDAAPRRLPKPSDHAPVVVEIDTQNL

Sequences:

>Translated_268_residues
MKIASWNVNSIRTRLEHVLDWLYTQSIDVLCLQETKVIDEDFPKASFEEEGYQVYVSGQKAYNGVALISRTTLSEVSSGF
TPLLGPDLTQHYDQQKRLITGIISPGVRILNLYVPNGSEIDSDKYVYKLEWLELLHHYVEKLLSQDPQHLLICGDFNIAL
DDRDMHDPSKREAHIMSTDRERQALQKVLALGLEDVFRKFNSKSEQFSWWNYRAGSFQRNKGWRIDHHYLTPTLYEQATA
CTIDAAPRRLPKPSDHAPVVVEIDTQNL
>Mature_268_residues
MKIASWNVNSIRTRLEHVLDWLYTQSIDVLCLQETKVIDEDFPKASFEEEGYQVYVSGQKAYNGVALISRTTLSEVSSGF
TPLLGPDLTQHYDQQKRLITGIISPGVRILNLYVPNGSEIDSDKYVYKLEWLELLHHYVEKLLSQDPQHLLICGDFNIAL
DDRDMHDPSKREAHIMSTDRERQALQKVLALGLEDVFRKFNSKSEQFSWWNYRAGSFQRNKGWRIDHHYLTPTLYEQATA
CTIDAAPRRLPKPSDHAPVVVEIDTQNL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:
GO:0003677: Exodeoxyribonuclease III
GO:0004518: Exodeoxyribonuclease III
GO:0004519: Exodeoxyribonuclease III
GO:0005622: Exodeoxyribonuclease III
GO:0006281: Exodeoxyribonuclease III

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=268, Percent_Identity=29.8507462686567, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI18375503, Length=268, Percent_Identity=29.8507462686567, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI18375501, Length=268, Percent_Identity=29.8507462686567, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=34.0740740740741, Blast_Score=134, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI71989536, Length=274, Percent_Identity=27.7372262773723, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI221330655, Length=238, Percent_Identity=29.8319327731092, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17136678, Length=238, Percent_Identity=29.8319327731092, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30935; Mature: 30935

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIASWNVNSIRTRLEHVLDWLYTQSIDVLCLQETKVIDEDFPKASFEEEGYQVYVSGQK
CEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCCCCCEEEEEECCC
AYNGVALISRTTLSEVSSGFTPLLGPDLTQHYDQQKRLITGIISPGVRILNLYVPNGSEI
CCCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
DSDKYVYKLEWLELLHHYVEKLLSQDPQHLLICGDFNIALDDRDMHDPSKREAHIMSTDR
CCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCCCCCCCCCHHCCCH
ERQALQKVLALGLEDVFRKFNSKSEQFSWWNYRAGSFQRNKGWRIDHHYLTPTLYEQATA
HHHHHHHHHHHCHHHHHHHHCCCCCCCEEEECCCCCEECCCCCEEECEECCHHHHHHHHE
CTIDAAPRRLPKPSDHAPVVVEIDTQNL
EEECCCCCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MKIASWNVNSIRTRLEHVLDWLYTQSIDVLCLQETKVIDEDFPKASFEEEGYQVYVSGQK
CEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCCCCCEEEEEECCC
AYNGVALISRTTLSEVSSGFTPLLGPDLTQHYDQQKRLITGIISPGVRILNLYVPNGSEI
CCCCEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
DSDKYVYKLEWLELLHHYVEKLLSQDPQHLLICGDFNIALDDRDMHDPSKREAHIMSTDR
CCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCCCCCCCCCHHCCCH
ERQALQKVLALGLEDVFRKFNSKSEQFSWWNYRAGSFQRNKGWRIDHHYLTPTLYEQATA
HHHHHHHHHHHCHHHHHHHHCCCCCCCEEEECCCCCEECCCCCEEECEECCHHHHHHHHE
CTIDAAPRRLPKPSDHAPVVVEIDTQNL
EEECCCCCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]