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The map label for this gene is menB [H]

Identifier: 158338362

GI number: 158338362

Start: 5327989

End: 5328822

Strand: Direct

Name: menB [H]

Synonym: AM1_5258

Alternate gene names: 158338362

Gene position: 5327989-5328822 (Clockwise)

Preceding gene: 158338361

Following gene: 158338364

Centisome position: 81.92

GC content: 49.52

Gene sequence:

>834_bases
ATGTCTTTCTCTTGGCAATCCGTCAAATCCTATCAAGATATCCTGTATCACAAAGCTGACGGCATTGCCAAAATCACCAT
TAATCGGCCCCATAAACGGAACGCCTTTCGGCCCCAAACAATTTTTGAACTTTATGATGCCTTTATCGACGCTCGAGAAG
ACCCGAGCCTTGGGGTTATTTTGCTCACTGGGGCCGGTCCCCATACAGATGGTAAATATGCGTTTTGCTCTGGTGGGGAT
CAAAGTATTCGAGGGGAAGCGGGCTATGTCGGCGATGATGGCGTTCCCCGTCTGAATGTTCTCGATCTTCAACGGTTAAT
TCGGTCGATCCCTAAAGTCGTAATTGCTCTTGTCGCAGGATATGCCATTGGTGGCGGACATGTCTTGCACTTGATCTGCG
ATCTATCCATTGCGGCAGACAATGCCATTTTTGGCCAAACAGGCCCCAAAGTGGGCAGCTTTGATGGCGGTTTTGGGGCG
AGCTATCTAGCCCGGGTGGTGGGCCAAAAGAAGGCCCGAGAAATATGGTTTCTCTGCCGCCAATACGATGCCCAAGCAGC
CTTGTCGATGGGCTTAGTGAATACAGTCGTTCCAGTCGCAGAACTCGAAGCGGAAGGGATTCAGTGGGCTCAAGAGATTT
TACAGAAAAGTCCGTTGGCCATCCGCTGTCTTAAAGCTGCTTTTAACGCAGATTGTGATGGGCAGGCCGGTATTCAAGAA
TTGGCTGGGAATGCCACGTTGTTGTTTTACATGAATGAAGAAAGTGCAGAGGGGAAAAATGCCTTTCTCGAAAAACGCGA
TCCGAATTTTCGGCAATTTCCTTGGCGGCCTTGA

Upstream 100 bases:

>100_bases
TGAAATATAAGATTCGGCGTGAAAAACCACTAAACTTCTTAGTTAAGACCTACGAAGGTGCTGTAATAGAATTTGCAGAA
GTTTCTAATTAGCGCAAGTC

Downstream 100 bases:

>100_bases
TGAGCCATGGGATGATCCTAAGCTGAAGAGCGGCATCCCATGTCAGCATTGGCTAATTACAGTTCAGGGAGCAAGTTTGA
CTTGCTCCCTGACGTTTAAA

Product: naphthoate synthase

Products: NA

Alternate protein names: DHNA-CoA synthase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSFSWQSVKSYQDILYHKADGIAKITINRPHKRNAFRPQTIFELYDAFIDAREDPSLGVILLTGAGPHTDGKYAFCSGGD
QSIRGEAGYVGDDGVPRLNVLDLQRLIRSIPKVVIALVAGYAIGGGHVLHLICDLSIAADNAIFGQTGPKVGSFDGGFGA
SYLARVVGQKKAREIWFLCRQYDAQAALSMGLVNTVVPVAELEAEGIQWAQEILQKSPLAIRCLKAAFNADCDGQAGIQE
LAGNATLLFYMNEESAEGKNAFLEKRDPNFRQFPWRP

Sequences:

>Translated_277_residues
MSFSWQSVKSYQDILYHKADGIAKITINRPHKRNAFRPQTIFELYDAFIDAREDPSLGVILLTGAGPHTDGKYAFCSGGD
QSIRGEAGYVGDDGVPRLNVLDLQRLIRSIPKVVIALVAGYAIGGGHVLHLICDLSIAADNAIFGQTGPKVGSFDGGFGA
SYLARVVGQKKAREIWFLCRQYDAQAALSMGLVNTVVPVAELEAEGIQWAQEILQKSPLAIRCLKAAFNADCDGQAGIQE
LAGNATLLFYMNEESAEGKNAFLEKRDPNFRQFPWRP
>Mature_276_residues
SFSWQSVKSYQDILYHKADGIAKITINRPHKRNAFRPQTIFELYDAFIDAREDPSLGVILLTGAGPHTDGKYAFCSGGDQ
SIRGEAGYVGDDGVPRLNVLDLQRLIRSIPKVVIALVAGYAIGGGHVLHLICDLSIAADNAIFGQTGPKVGSFDGGFGAS
YLARVVGQKKAREIWFLCRQYDAQAALSMGLVNTVVPVAELEAEGIQWAQEILQKSPLAIRCLKAAFNADCDGQAGIQEL
AGNATLLFYMNEESAEGKNAFLEKRDPNFRQFPWRP

Specific function: Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4- dihydroxy-2-naphthoyl-CoA (DHNA-CoA) [H]

COG id: COG0447

COG function: function code H; Dihydroxynaphthoic acid synthase

Gene ontology:
GO:0003824: Naphthoate synthase
GO:0008152: Naphthoate synthase
GO:0008935: Naphthoate synthase
GO:0009234: Naphthoate synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=32.5670498084291, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=31.5175097276265, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI213417737, Length=227, Percent_Identity=25.5506607929515, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI157694516, Length=227, Percent_Identity=25.5506607929515, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=26.1467889908257, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=26.1467889908257, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=26.1467889908257, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=28.3582089552239, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI70995211, Length=234, Percent_Identity=29.4871794871795, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1788597, Length=271, Percent_Identity=68.6346863468635, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=33.7121212121212, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=31.4393939393939, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=32.3420074349442, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=29.8507462686567, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788682, Length=212, Percent_Identity=27.3584905660377, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=32.5581395348837, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=29.9212598425197, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=219, Percent_Identity=28.7671232876712, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=193, Percent_Identity=31.6062176165803, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=214, Percent_Identity=26.6355140186916, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=225, Percent_Identity=27.1111111111111, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20129971, Length=270, Percent_Identity=30.7407407407407, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653477, Length=270, Percent_Identity=30.7407407407407, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653139, Length=272, Percent_Identity=27.5735294117647, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357171, Length=195, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922422, Length=271, Percent_Identity=25.0922509225092, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376
- InterPro:   IPR010198 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.1.3.36 [H]

Molecular weight: Translated: 30237; Mature: 30106

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSWQSVKSYQDILYHKADGIAKITINRPHKRNAFRPQTIFELYDAFIDAREDPSLGVI
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
LLTGAGPHTDGKYAFCSGGDQSIRGEAGYVGDDGVPRLNVLDLQRLIRSIPKVVIALVAG
EEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YAIGGGHVLHLICDLSIAADNAIFGQTGPKVGSFDGGFGASYLARVVGQKKAREIWFLCR
HCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QYDAQAALSMGLVNTVVPVAELEAEGIQWAQEILQKSPLAIRCLKAAFNADCDGQAGIQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHH
LAGNATLLFYMNEESAEGKNAFLEKRDPNFRQFPWRP
HCCCEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFSWQSVKSYQDILYHKADGIAKITINRPHKRNAFRPQTIFELYDAFIDAREDPSLGVI
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
LLTGAGPHTDGKYAFCSGGDQSIRGEAGYVGDDGVPRLNVLDLQRLIRSIPKVVIALVAG
EEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YAIGGGHVLHLICDLSIAADNAIFGQTGPKVGSFDGGFGASYLARVVGQKKAREIWFLCR
HCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QYDAQAALSMGLVNTVVPVAELEAEGIQWAQEILQKSPLAIRCLKAAFNADCDGQAGIQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHH
LAGNATLLFYMNEESAEGKNAFLEKRDPNFRQFPWRP
HCCCEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]