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The map label for this gene is ruvC

Identifier: 158338360

GI number: 158338360

Start: 5326700

End: 5327170

Strand: Reverse

Name: ruvC

Synonym: AM1_5256

Alternate gene names: 158338360

Gene position: 5327170-5326700 (Counterclockwise)

Preceding gene: 158338363

Following gene: 158338358

Centisome position: 81.91

GC content: 46.28

Gene sequence:

>471_bases
GTGACAAAAAAGATTTTGGGTTTAGATCCGGGATTAGCGTCCTTAGGCTTCGGGGTGATTTGTACAGAGTCTGATAACCT
TGAACTCCTTGACTTCGGAATTATTCAAACACCAGCCAAAACAGATATTGGGAAACGATTGCAGATTATTTATGAGGACT
TACATCAAGTTCTCAACACCCATCAGCCTGATGTGGTGGCGATAGAAAAACTTTTCTTCTATCGTATGGGCAATACGATT
TTGGTGGCTCAAGCTCGTGGGGTTGTCTTGCTGGTCTTAGCACAGCATCAGCTTCCCATTATTGAATACACGCCGGCCCA
GATTAAGTTGGCTCTAACTGGATATGGCGCAGCAGATAAAGTTGCTGTGCAAGAAGCCGTTGCCAGAGAACTCTGCTTAG
ATTGCTTGCCTCGGCCAGATGACGCGGCAGATGCTCTAGCAGTTGCCCTGACCGCATGGTTTCAACGATAA

Upstream 100 bases:

>100_bases
GATAGAGAATCGAGGCCAATGTGGGGCGTAAACCTATTCTGAAGCCCTCATGACTGTAGCCTATTGTCATACGTTGTGCT
TAAAGGCTGCTCTATCAATA

Downstream 100 bases:

>100_bases
GCGCTAATATGCATCAAAATATGGGCTGACGTCCCCCATCAAACTAACTGGATTATAGCCAAAGCATCAAAAAAAATTGA
TACTGACTCCAAACTTTTTT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTKKILGLDPGLASLGFGVICTESDNLELLDFGIIQTPAKTDIGKRLQIIYEDLHQVLNTHQPDVVAIEKLFFYRMGNTI
LVAQARGVVLLVLAQHQLPIIEYTPAQIKLALTGYGAADKVAVQEAVARELCLDCLPRPDDAADALAVALTAWFQR

Sequences:

>Translated_156_residues
MTKKILGLDPGLASLGFGVICTESDNLELLDFGIIQTPAKTDIGKRLQIIYEDLHQVLNTHQPDVVAIEKLFFYRMGNTI
LVAQARGVVLLVLAQHQLPIIEYTPAQIKLALTGYGAADKVAVQEAVARELCLDCLPRPDDAADALAVALTAWFQR
>Mature_155_residues
TKKILGLDPGLASLGFGVICTESDNLELLDFGIIQTPAKTDIGKRLQIIYEDLHQVLNTHQPDVVAIEKLFFYRMGNTIL
VAQARGVVLLVLAQHQLPIIEYTPAQIKLALTGYGAADKVAVQEAVARELCLDCLPRPDDAADALAVALTAWFQR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=135, Percent_Identity=36.2962962962963, Blast_Score=85, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_ACAM1 (B0C9S0)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001519537.1
- ProteinModelPortal:   B0C9S0
- SMR:   B0C9S0
- GeneID:   5684052
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_5256
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- ProtClustDB:   PRK00039
- BioCyc:   AMAR329726:AM1_5256-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17039; Mature: 16908

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKILGLDPGLASLGFGVICTESDNLELLDFGIIQTPAKTDIGKRLQIIYEDLHQVLNT
CCCCCCCCCCCHHHHCCCEEEECCCCEEEEEECEECCCCCCCHHHHHHHHHHHHHHHHCC
HQPDVVAIEKLFFYRMGNTILVAQARGVVLLVLAQHQLPIIEYTPAQIKLALTGYGAADK
CCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCEEEECCCEEEEEEECCCCCHH
VAVQEAVARELCLDCLPRPDDAADALAVALTAWFQR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKKILGLDPGLASLGFGVICTESDNLELLDFGIIQTPAKTDIGKRLQIIYEDLHQVLNT
CCCCCCCCCCHHHHCCCEEEECCCCEEEEEECEECCCCCCCHHHHHHHHHHHHHHHHCC
HQPDVVAIEKLFFYRMGNTILVAQARGVVLLVLAQHQLPIIEYTPAQIKLALTGYGAADK
CCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCEEEECCCEEEEEEECCCCCHH
VAVQEAVARELCLDCLPRPDDAADALAVALTAWFQR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA