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The map label for this gene is icaA [H]

Identifier: 158338314

GI number: 158338314

Start: 5272127

End: 5273605

Strand: Direct

Name: icaA [H]

Synonym: AM1_5210

Alternate gene names: 158338314

Gene position: 5272127-5273605 (Clockwise)

Preceding gene: 158338312

Following gene: 158338318

Centisome position: 81.06

GC content: 48.28

Gene sequence:

>1479_bases
ATGGGCATTAGTTTCATTGATGGCATTTCAGGTTTTATTCCTGAGCTATACCTTGGTATTTTGACGCTGATCGCCATATA
CAGCTTCCACAAAATTTCGATTATTTGGCGTTATTATCTGCATCGTCGTCGAGAAATTTCACCCCTACACAAATTCTCCG
ACGCTGACCTGCCCCAGGTGACCATTCAGCTCCCATTATTTAATGAAATGTATGTGGTCGATCGGCTCCTAGAGGCCGTT
GCTGCCCTGGAATATCCCGTTGATAAACTGCAAATTCAGGTCTTAGATGACTCGACGGATGAAACGCGAGAGATTTGTCG
AGCAAAGGTGCGAGAACTGAAGCAGCGCCACCTCAATATTGACTACATCCATCGCTGCGATCGCAAAGGGTATAAGGCTG
GAGCCCTAGCCTATGGACTCCAGTCCGCCACCGGGGACTTGGTGATGATCTTTGATGCTGATTTTGTCCCGTCCCCCGAT
ACCTTAATCAACATGGTTCACTATTTTGCCAACCCAAAGGTGGGCATGGTGCAAGCCCGCTGGGGACATATTAATCGCCA
CTATTCCATCCTCACAGAAATCCAAGCCCTAATGTTGGATGGGCATTTTGTCACGGAACAAACATCTCGCAATCGGTCCG
GGTGTTTTTTCAATTTCAACGGCACGGCAGGGATTTGGCGGATTCAAACCATCGAAGATGCGGGAGGCTGGCAGCATACC
ACCGTAACGGAGGATTTGGATCTGTCTTATCGAGCCCAGCTCAAAGGATGGGAGTGCATTTACCTCCCTAATATTGTGGT
GCCTGCCGAATTGCCCATGGAGATGAATTCCTTCAAGTCTCAGCAATTTCGATGGGCCAAAGGAGCCAGTCAAGTGGCGA
AAAAGCTGCTGCTACCGATTTTGACCTCGAATGCTCCTGGACATGTAAAGTTAGAAGCCTTTTTTCACCTCACCAATAAC
TTCAACTACCTGCTGTTATTGGTTCTACTGCTGTTATCGTTGCCTTACCAGCTCTTTCTCGCCGAAACGGGCTGGCGTTA
TGGTCTAGCTATCCACCTGCCGTTATTTCTGATTACTACCCTCAGTCTATTGGCCTTTTACTCCGTGGCTCAAGAGGAAC
AACGGGGTCAAAATTCGCCCTGGAAACTCACTTCAAACCTATTTTTACTGATGAGTGTGGGGATTGGCCTCAGTATCAAT
CAATCCCTAGCCGTTTATGATGGCCTATTTCGGGTGGGTCGCGATTTTGTCCGCACGCCTAAGCATGGCGTTACGAGCAA
CGAAGAGGATTGGAAAACCAGAAAATACCGCGCCGCCAGAAATTTAGTGCCCTACCTGGAGCTGTCTATGGTGGTGTATC
TACTGCTGACCATTAGCGTAGCGGTCGTCAACGCCCATTACCTATCTCTCCCATTTCTACTGCTGTTCTTGATCGGCTAT
ATCTATGTGCTTTCCCTTAGCATGTTTCAACGCCGCTAA

Upstream 100 bases:

>100_bases
CTGGGCGAGATAAAATACAGTCCTCCACCTCCTTAAACAGGTCAAGGGGACAAGAATTTATTACAAATTCTGAGTATATT
CAATCAACGAAAAGCATTAT

Downstream 100 bases:

>100_bases
GTCTGCCTAGTTTGGCCCAGCTCGACCGAGGAAAATCGGAGCCGCCGGGGATAAGAGAACGATAAAAAGTGGCCTAGCCC
CTAAAACAACTTGTGATTTC

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MGISFIDGISGFIPELYLGILTLIAIYSFHKISIIWRYYLHRRREISPLHKFSDADLPQVTIQLPLFNEMYVVDRLLEAV
AALEYPVDKLQIQVLDDSTDETREICRAKVRELKQRHLNIDYIHRCDRKGYKAGALAYGLQSATGDLVMIFDADFVPSPD
TLINMVHYFANPKVGMVQARWGHINRHYSILTEIQALMLDGHFVTEQTSRNRSGCFFNFNGTAGIWRIQTIEDAGGWQHT
TVTEDLDLSYRAQLKGWECIYLPNIVVPAELPMEMNSFKSQQFRWAKGASQVAKKLLLPILTSNAPGHVKLEAFFHLTNN
FNYLLLLVLLLLSLPYQLFLAETGWRYGLAIHLPLFLITTLSLLAFYSVAQEEQRGQNSPWKLTSNLFLLMSVGIGLSIN
QSLAVYDGLFRVGRDFVRTPKHGVTSNEEDWKTRKYRAARNLVPYLELSMVVYLLLTISVAVVNAHYLSLPFLLLFLIGY
IYVLSLSMFQRR

Sequences:

>Translated_492_residues
MGISFIDGISGFIPELYLGILTLIAIYSFHKISIIWRYYLHRRREISPLHKFSDADLPQVTIQLPLFNEMYVVDRLLEAV
AALEYPVDKLQIQVLDDSTDETREICRAKVRELKQRHLNIDYIHRCDRKGYKAGALAYGLQSATGDLVMIFDADFVPSPD
TLINMVHYFANPKVGMVQARWGHINRHYSILTEIQALMLDGHFVTEQTSRNRSGCFFNFNGTAGIWRIQTIEDAGGWQHT
TVTEDLDLSYRAQLKGWECIYLPNIVVPAELPMEMNSFKSQQFRWAKGASQVAKKLLLPILTSNAPGHVKLEAFFHLTNN
FNYLLLLVLLLLSLPYQLFLAETGWRYGLAIHLPLFLITTLSLLAFYSVAQEEQRGQNSPWKLTSNLFLLMSVGIGLSIN
QSLAVYDGLFRVGRDFVRTPKHGVTSNEEDWKTRKYRAARNLVPYLELSMVVYLLLTISVAVVNAHYLSLPFLLLFLIGY
IYVLSLSMFQRR
>Mature_491_residues
GISFIDGISGFIPELYLGILTLIAIYSFHKISIIWRYYLHRRREISPLHKFSDADLPQVTIQLPLFNEMYVVDRLLEAVA
ALEYPVDKLQIQVLDDSTDETREICRAKVRELKQRHLNIDYIHRCDRKGYKAGALAYGLQSATGDLVMIFDADFVPSPDT
LINMVHYFANPKVGMVQARWGHINRHYSILTEIQALMLDGHFVTEQTSRNRSGCFFNFNGTAGIWRIQTIEDAGGWQHTT
VTEDLDLSYRAQLKGWECIYLPNIVVPAELPMEMNSFKSQQFRWAKGASQVAKKLLLPILTSNAPGHVKLEAFFHLTNNF
NYLLLLVLLLLSLPYQLFLAETGWRYGLAIHLPLFLITTLSLLAFYSVAQEEQRGQNSPWKLTSNLFLLMSVGIGLSINQ
SLAVYDGLFRVGRDFVRTPKHGVTSNEEDWKTRKYRAARNLVPYLELSMVVYLLLTISVAVVNAHYLSLPFLLLFLIGYI
YVLSLSMFQRR

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=376, Percent_Identity=25.531914893617, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082284, Length=306, Percent_Identity=26.797385620915, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 56384; Mature: 56253

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISFIDGISGFIPELYLGILTLIAIYSFHKISIIWRYYLHRRREISPLHKFSDADLPQV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEE
TIQLPLFNEMYVVDRLLEAVAALEYPVDKLQIQVLDDSTDETREICRAKVRELKQRHLNI
EEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCH
DYIHRCDRKGYKAGALAYGLQSATGDLVMIFDADFVPSPDTLINMVHYFANPKVGMVQAR
HHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEC
WGHINRHYSILTEIQALMLDGHFVTEQTSRNRSGCFFNFNGTAGIWRIQTIEDAGGWQHT
CCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCC
TVTEDLDLSYRAQLKGWECIYLPNIVVPAELPMEMNSFKSQQFRWAKGASQVAKKLLLPI
CCCCCCCCCHHHCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
LTSNAPGHVKLEAFFHLTNNFNYLLLLVLLLLSLPYQLFLAETGWRYGLAIHLPLFLITT
HCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHEEEECCCCCEEHHHHHHHHHHHH
LSLLAFYSVAQEEQRGQNSPWKLTSNLFLLMSVGIGLSINQSLAVYDGLFRVGRDFVRTP
HHHHHHHHHHHHHHCCCCCCCEEHHHHHEEECCCCCCCCCCHHHHHHHHHHHCHHHHCCC
KHGVTSNEEDWKTRKYRAARNLVPYLELSMVVYLLLTISVAVVNAHYLSLPFLLLFLIGY
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYVLSLSMFQRR
HHHHHHHHHHCC
>Mature Secondary Structure 
GISFIDGISGFIPELYLGILTLIAIYSFHKISIIWRYYLHRRREISPLHKFSDADLPQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEE
TIQLPLFNEMYVVDRLLEAVAALEYPVDKLQIQVLDDSTDETREICRAKVRELKQRHLNI
EEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCH
DYIHRCDRKGYKAGALAYGLQSATGDLVMIFDADFVPSPDTLINMVHYFANPKVGMVQAR
HHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEC
WGHINRHYSILTEIQALMLDGHFVTEQTSRNRSGCFFNFNGTAGIWRIQTIEDAGGWQHT
CCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCC
TVTEDLDLSYRAQLKGWECIYLPNIVVPAELPMEMNSFKSQQFRWAKGASQVAKKLLLPI
CCCCCCCCCHHHCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
LTSNAPGHVKLEAFFHLTNNFNYLLLLVLLLLSLPYQLFLAETGWRYGLAIHLPLFLITT
HCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHEEEECCCCCEEHHHHHHHHHHHH
LSLLAFYSVAQEEQRGQNSPWKLTSNLFLLMSVGIGLSINQSLAVYDGLFRVGRDFVRTP
HHHHHHHHHHHHHHCCCCCCCEEHHHHHEEECCCCCCCCCCHHHHHHHHHHHCHHHHCCC
KHGVTSNEEDWKTRKYRAARNLVPYLELSMVVYLLLTISVAVVNAHYLSLPFLLLFLIGY
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYVLSLSMFQRR
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA