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The map label for this gene is potD-B [H]

Identifier: 158338236

GI number: 158338236

Start: 5190008

End: 5191123

Strand: Direct

Name: potD-B [H]

Synonym: AM1_5133

Alternate gene names: 158338236

Gene position: 5190008-5191123 (Clockwise)

Preceding gene: 158338235

Following gene: 158338239

Centisome position: 79.8

GC content: 47.94

Gene sequence:

>1116_bases
ATGGATCGACGTTCTTTTTTACAGACCTTAGGTGCAGTAACCTTGAGTCAATTATTAGGGGCCTGTCAACAGAACAACCG
CACCGCATTACAAGTAAATTTGTTGCAGAAGACCTTACCCCCTCAATTGGTAGATAAATTCCGCTCAAACTCAAATATTC
CTTTACAAATTTCCCTTAAATCCTCTACCCAGGAAATTTTTACTCAGCTCCAAAAATGGAACAAAGAAGATCAAAAGCAA
AGGTCTGAGAAAGTACCTTCAGAAGATCAACCCAAACTCCCATCCCGATTAGCCAGCCTAGGAGATTACTGGTTACCCCA
AGCGATCCAGCAAAACCTAATCCAACCCCTATCCGCCGATGATCTGAATCGATGGTCCGCCTTAGCGCCTCAATGGCAGC
AGCTCGTTCGTCGCGATCTGCAGGGCAATCCCAGCGCCGAAGGCAAAATTTGGGGGGCTCCCTATCGGTGGGGGGCCACC
ATGATTGCCTTTCGAAAAGACAAATTCAGAGATTTAGGATGGCAACCCCAAGATTGGTCAGACTTATGGCGCTCTGAACT
GAAGGAGCACATTAGCCTCCTCGATCAACCCCGAGAGGTCATTGGTCTTACCCTCAAAAAACTGGGCCAGTCCTACAACT
TAGAGGATCTAGACACCATTTCAGAACTCCCCCAAACCCTGAAGGAACTGAATGAGCAAACCCTCTTTTATGCCTCTAGC
CAATATTTAGAGCCCCTAATCTTGGGAGATACCTGGGCTGCGGTGGGATGGTCATCTGACATATTACCCACTCTAGCCCG
AGAACCTGATTTGGAAGCAGTCTTCCCTGCATCTGGCTCAGCCTTGTGGGCGGATCTATGGGTCCAAGTCCAACCGAGTG
AAACGATTAATCAATGGATTAATTACTGGTGGGAAGACACCGTTGCCAATAATTTATCCAAATTTACAGATACCATCTCC
CCGCTGTTATCCACCACACTTCCGGTCAAGTCGCAAAGCCATCGACTTGTTGCCGCTCCTAAGCAGTTCGAAAGGAGTGA
GTTCTTGTTCCCCCTCTCACCACCCACCCTGCAACAATACGAACGTCTGTGGCAAGCTATGCGAATACCAGTCTAA

Upstream 100 bases:

>100_bases
GCCACTTCCCTGATGGCATGCATTATCGACCGTTTGAGTCTTCAAACATCGGTTTCCAATCCAGCAGCGTTATCCTAATA
GAGGTGATGAATAAAAAGTG

Downstream 100 bases:

>100_bases
AACACACTTCGAGGATAATTTGGGTTTAACCAAAGCCGACAAGGTAAAGCCTGGGTGGACTTAGGATCTGCATAGTCCGG
AATCGGTTGAGATCGTTCAT

Product: ABC transporter periplasmic solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MDRRSFLQTLGAVTLSQLLGACQQNNRTALQVNLLQKTLPPQLVDKFRSNSNIPLQISLKSSTQEIFTQLQKWNKEDQKQ
RSEKVPSEDQPKLPSRLASLGDYWLPQAIQQNLIQPLSADDLNRWSALAPQWQQLVRRDLQGNPSAEGKIWGAPYRWGAT
MIAFRKDKFRDLGWQPQDWSDLWRSELKEHISLLDQPREVIGLTLKKLGQSYNLEDLDTISELPQTLKELNEQTLFYASS
QYLEPLILGDTWAAVGWSSDILPTLAREPDLEAVFPASGSALWADLWVQVQPSETINQWINYWWEDTVANNLSKFTDTIS
PLLSTTLPVKSQSHRLVAAPKQFERSEFLFPLSPPTLQQYERLWQAMRIPV

Sequences:

>Translated_371_residues
MDRRSFLQTLGAVTLSQLLGACQQNNRTALQVNLLQKTLPPQLVDKFRSNSNIPLQISLKSSTQEIFTQLQKWNKEDQKQ
RSEKVPSEDQPKLPSRLASLGDYWLPQAIQQNLIQPLSADDLNRWSALAPQWQQLVRRDLQGNPSAEGKIWGAPYRWGAT
MIAFRKDKFRDLGWQPQDWSDLWRSELKEHISLLDQPREVIGLTLKKLGQSYNLEDLDTISELPQTLKELNEQTLFYASS
QYLEPLILGDTWAAVGWSSDILPTLAREPDLEAVFPASGSALWADLWVQVQPSETINQWINYWWEDTVANNLSKFTDTIS
PLLSTTLPVKSQSHRLVAAPKQFERSEFLFPLSPPTLQQYERLWQAMRIPV
>Mature_371_residues
MDRRSFLQTLGAVTLSQLLGACQQNNRTALQVNLLQKTLPPQLVDKFRSNSNIPLQISLKSSTQEIFTQLQKWNKEDQKQ
RSEKVPSEDQPKLPSRLASLGDYWLPQAIQQNLIQPLSADDLNRWSALAPQWQQLVRRDLQGNPSAEGKIWGAPYRWGAT
MIAFRKDKFRDLGWQPQDWSDLWRSELKEHISLLDQPREVIGLTLKKLGQSYNLEDLDTISELPQTLKELNEQTLFYASS
QYLEPLILGDTWAAVGWSSDILPTLAREPDLEAVFPASGSALWADLWVQVQPSETINQWINYWWEDTVANNLSKFTDTIS
PLLSTTLPVKSQSHRLVAAPKQFERSEFLFPLSPPTLQQYERLWQAMRIPV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=270, Percent_Identity=22.962962962963, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787078, Length=235, Percent_Identity=25.531914893617, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42620; Mature: 42620

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRSFLQTLGAVTLSQLLGACQQNNRTALQVNLLQKTLPPQLVDKFRSNSNIPLQISLK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCHHHHHHHHCCCCCCEEEEEC
SSTQEIFTQLQKWNKEDQKQRSEKVPSEDQPKLPSRLASLGDYWLPQAIQQNLIQPLSAD
CHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCC
DLNRWSALAPQWQQLVRRDLQGNPSAEGKIWGAPYRWGATMIAFRKDKFRDLGWQPQDWS
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCHH
DLWRSELKEHISLLDQPREVIGLTLKKLGQSYNLEDLDTISELPQTLKELNEQTLFYASS
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHH
QYLEPLILGDTWAAVGWSSDILPTLAREPDLEAVFPASGSALWADLWVQVQPSETINQWI
HHCCEEEECCCHHHCCCCCCHHHHHCCCCCCCEECCCCCCEEEEEHEEEECCHHHHHHHH
NYWWEDTVANNLSKFTDTISPLLSTTLPVKSQSHRLVAAPKQFERSEFLFPLSPPTLQQY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHCCCCCCCCCCCHHHHHH
ERLWQAMRIPV
HHHHHHHCCCC
>Mature Secondary Structure
MDRRSFLQTLGAVTLSQLLGACQQNNRTALQVNLLQKTLPPQLVDKFRSNSNIPLQISLK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCHHHHHHHHCCCCCCEEEEEC
SSTQEIFTQLQKWNKEDQKQRSEKVPSEDQPKLPSRLASLGDYWLPQAIQQNLIQPLSAD
CHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCC
DLNRWSALAPQWQQLVRRDLQGNPSAEGKIWGAPYRWGATMIAFRKDKFRDLGWQPQDWS
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCHH
DLWRSELKEHISLLDQPREVIGLTLKKLGQSYNLEDLDTISELPQTLKELNEQTLFYASS
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHH
QYLEPLILGDTWAAVGWSSDILPTLAREPDLEAVFPASGSALWADLWVQVQPSETINQWI
HHCCEEEECCCHHHCCCCCCHHHHHCCCCCCCEECCCCCCEEEEEHEEEECCHHHHHHHH
NYWWEDTVANNLSKFTDTISPLLSTTLPVKSQSHRLVAAPKQFERSEFLFPLSPPTLQQY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHCCCCCCCCCCCHHHHHH
ERLWQAMRIPV
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]