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The map label for this gene is atsB [H]

Identifier: 158338131

GI number: 158338131

Start: 5066153

End: 5067514

Strand: Direct

Name: atsB [H]

Synonym: AM1_5023

Alternate gene names: 158338131

Gene position: 5066153-5067514 (Clockwise)

Preceding gene: 158338130

Following gene: 158338132

Centisome position: 77.9

GC content: 40.46

Gene sequence:

>1362_bases
ATGATACAAATCATGTCCCAATACTCCTCTAAGTTAAAGATGCCGAATGTACATCTGTTTCGAGCAGAAGAGCATTCTAT
GCTGCTATCTATTGAGCAAGGTTCAATATATGAAATCGATGATATTTACGCTGAGATGCTCGATCGATGGATGGAATTTG
GTGAAGTAGAAAGAGTGCATCAAGTATTATCGGCAAAGGGAATACATCTGAATTCAGTTCCTCCTGAGGCTCCACCTAAA
TCTATTCCAATTCATGCACTCTCTCTAGCCATAGCTCAAAAATGTAATCTTGGTTGCACCTACTGCTATGCCCATCAAGG
TAGCTTTGGTGGCAATGAAGGTAGTATGTCTTTTGAGGTCGCGAGGAATTCTATTGATCTCTTGTTGAAAGGAACAGAAT
CTGGAGAAACAGTTTCCTTAGCCTATTTAGGAGGAGAACCTTTAGCCAATCGTTCTGTTTTGCAGAAAACAACAGAATAC
ACTATTGCCAAAGCTTCTTCAGTGGGAATTAAGACAAACTTCTCATTGACAACGAATGGAACATTGTTGACTGTTGAAGA
TGCAGAGTTTTTTAATAATCATGGCTTTTCTGTCACGATTAGCATTGATGGAAATCAAGAAAAACATGATTTCCTAAGAC
CATTTAAATCAGGAAAGGGAAGTTACAAAACGGTCATAGAAAAAGTAAAACTTTTATTAGATATTCCTCACCGACGTTGT
CGAGTATCCGCAAGAGTTACAGTGACACCTGAAAACTTGTGTTTGGAAGAGACTCTGGACAAACTCGTTTCACTCGGCTT
TGATAATGTCATGTTCTCTCCAGTTCTGAAATCACCAACTGGCCTACATCAAATGGGAGAATTTGAATTTGAAGTGCTGC
TAAATCAAATGATAGAGTGTGGCCAAAAATTTGAGCAGAATTTTGAAGATCACAAGATCTATCCCTTTGCAAATATACTG
AACACGCTTCAACGAATACATCATTATAGATGTGACGCTTACCCCTGTGGCGCAGGAGGAGCCTATTTGGGTGTTTCTTC
AAAGGGAGACTTGTATGCTTGTCATCGATTTGTTGATGATGCTCAAGGTTCAATGGGAAATATCGTAGATGGTGTTGATC
CTGCTAAGCAAGAGAATTGGCTGGCGGCCAGAAATGTGCATACTCAGGAACCATGCAAGGCTTGCTGGGCACGATACTTG
TGTGGAGGCGGTTGTCATCATGAATCAATTTACAGAGGTCGACCTGCCTGCGATTACATTCGCGGTTGGTTGCATTACTG
TTTAGGCGTATATGTCAAGCTTCTTAAGATTAATCCTTCACTCCTGGATCAGATTTTGAACAACCAATCTGTTTCTGGAT
AG

Upstream 100 bases:

>100_bases
AATCAGTTGCTAATCCTTTACTGGAGAATCTTTCTTAGGCCATGGATAGGTGATTGCAACTGATTCTCCTTCGATACTAA
TCATTGATTGTAATCAGGCA

Downstream 100 bases:

>100_bases
CTATCGAAATGGGAACCAAGGACCAAACCACTTTTGATGTCTGTGTGATCGGTGGTGGTCCTGCAGGCTCAGTAGTATCC
CATCGTTTGGCATCGTTAGG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: AnSME; Arylsulfatase-activating protein; Ser-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MIQIMSQYSSKLKMPNVHLFRAEEHSMLLSIEQGSIYEIDDIYAEMLDRWMEFGEVERVHQVLSAKGIHLNSVPPEAPPK
SIPIHALSLAIAQKCNLGCTYCYAHQGSFGGNEGSMSFEVARNSIDLLLKGTESGETVSLAYLGGEPLANRSVLQKTTEY
TIAKASSVGIKTNFSLTTNGTLLTVEDAEFFNNHGFSVTISIDGNQEKHDFLRPFKSGKGSYKTVIEKVKLLLDIPHRRC
RVSARVTVTPENLCLEETLDKLVSLGFDNVMFSPVLKSPTGLHQMGEFEFEVLLNQMIECGQKFEQNFEDHKIYPFANIL
NTLQRIHHYRCDAYPCGAGGAYLGVSSKGDLYACHRFVDDAQGSMGNIVDGVDPAKQENWLAARNVHTQEPCKACWARYL
CGGGCHHESIYRGRPACDYIRGWLHYCLGVYVKLLKINPSLLDQILNNQSVSG

Sequences:

>Translated_453_residues
MIQIMSQYSSKLKMPNVHLFRAEEHSMLLSIEQGSIYEIDDIYAEMLDRWMEFGEVERVHQVLSAKGIHLNSVPPEAPPK
SIPIHALSLAIAQKCNLGCTYCYAHQGSFGGNEGSMSFEVARNSIDLLLKGTESGETVSLAYLGGEPLANRSVLQKTTEY
TIAKASSVGIKTNFSLTTNGTLLTVEDAEFFNNHGFSVTISIDGNQEKHDFLRPFKSGKGSYKTVIEKVKLLLDIPHRRC
RVSARVTVTPENLCLEETLDKLVSLGFDNVMFSPVLKSPTGLHQMGEFEFEVLLNQMIECGQKFEQNFEDHKIYPFANIL
NTLQRIHHYRCDAYPCGAGGAYLGVSSKGDLYACHRFVDDAQGSMGNIVDGVDPAKQENWLAARNVHTQEPCKACWARYL
CGGGCHHESIYRGRPACDYIRGWLHYCLGVYVKLLKINPSLLDQILNNQSVSG
>Mature_453_residues
MIQIMSQYSSKLKMPNVHLFRAEEHSMLLSIEQGSIYEIDDIYAEMLDRWMEFGEVERVHQVLSAKGIHLNSVPPEAPPK
SIPIHALSLAIAQKCNLGCTYCYAHQGSFGGNEGSMSFEVARNSIDLLLKGTESGETVSLAYLGGEPLANRSVLQKTTEY
TIAKASSVGIKTNFSLTTNGTLLTVEDAEFFNNHGFSVTISIDGNQEKHDFLRPFKSGKGSYKTVIEKVKLLLDIPHRRC
RVSARVTVTPENLCLEETLDKLVSLGFDNVMFSPVLKSPTGLHQMGEFEFEVLLNQMIECGQKFEQNFEDHKIYPFANIL
NTLQRIHHYRCDAYPCGAGGAYLGVSSKGDLYACHRFVDDAQGSMGNIVDGVDPAKQENWLAARNVHTQEPCKACWARYL
CGGGCHHESIYRGRPACDYIRGWLHYCLGVYVKLLKINPSLLDQILNNQSVSG

Specific function: Involved in 'Ser-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of serine ('Ser-72' in the arylsulfatase AtsA) into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemica

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994973, Length=392, Percent_Identity=23.7244897959184, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081923, Length=348, Percent_Identity=27.0114942528736, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 50486; Mature: 50486

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIMSQYSSKLKMPNVHLFRAEEHSMLLSIEQGSIYEIDDIYAEMLDRWMEFGEVERVH
CCEEHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHH
QVLSAKGIHLNSVPPEAPPKSIPIHALSLAIAQKCNLGCTYCYAHQGSFGGNEGSMSFEV
HHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEHEE
ARNSIDLLLKGTESGETVSLAYLGGEPLANRSVLQKTTEYTIAKASSVGIKTNFSLTTNG
HHCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHEEECCCCCEEEEEEEEECC
TLLTVEDAEFFNNHGFSVTISIDGNQEKHDFLRPFKSGKGSYKTVIEKVKLLLDIPHRRC
EEEEEECHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHE
RVSARVTVTPENLCLEETLDKLVSLGFDNVMFSPVLKSPTGLHQMGEFEFEVLLNQMIEC
EEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHH
GQKFEQNFEDHKIYPFANILNTLQRIHHYRCDAYPCGAGGAYLGVSSKGDLYACHRFVDD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHH
AQGSMGNIVDGVDPAKQENWLAARNVHTQEPCKACWARYLCGGGCHHESIYRGRPACDYI
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHH
RGWLHYCLGVYVKLLKINPSLLDQILNNQSVSG
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIQIMSQYSSKLKMPNVHLFRAEEHSMLLSIEQGSIYEIDDIYAEMLDRWMEFGEVERVH
CCEEHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHH
QVLSAKGIHLNSVPPEAPPKSIPIHALSLAIAQKCNLGCTYCYAHQGSFGGNEGSMSFEV
HHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEHEE
ARNSIDLLLKGTESGETVSLAYLGGEPLANRSVLQKTTEYTIAKASSVGIKTNFSLTTNG
HHCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHEEECCCCCEEEEEEEEECC
TLLTVEDAEFFNNHGFSVTISIDGNQEKHDFLRPFKSGKGSYKTVIEKVKLLLDIPHRRC
EEEEEECHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHE
RVSARVTVTPENLCLEETLDKLVSLGFDNVMFSPVLKSPTGLHQMGEFEFEVLLNQMIEC
EEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHH
GQKFEQNFEDHKIYPFANILNTLQRIHHYRCDAYPCGAGGAYLGVSSKGDLYACHRFVDD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHH
AQGSMGNIVDGVDPAKQENWLAARNVHTQEPCKACWARYLCGGGCHHESIYRGRPACDYI
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHH
RGWLHYCLGVYVKLLKINPSLLDQILNNQSVSG
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180918 [H]