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The map label for this gene is azoR2 [H]

Identifier: 158338032

GI number: 158338032

Start: 4965479

End: 4966117

Strand: Reverse

Name: azoR2 [H]

Synonym: AM1_4919

Alternate gene names: 158338032

Gene position: 4966117-4965479 (Counterclockwise)

Preceding gene: 158338034

Following gene: 158338027

Centisome position: 76.36

GC content: 47.26

Gene sequence:

>639_bases
ATGCTTTCTCCAGACTCCAAGCCCCGTTGCTTACTCCACATGGATGTGAGTGCCCGCGTCCAAGGCTCTTACTCCCGCCA
GCTTACCCAAAAATTTGTCTCTCAATGGCAACAGGCCAACCCCCATCACCAGATCATTTATCGCGATATTGGCCGTTTCC
CCATCCCCCATATTGATGAGACTTGGGTTGCAGCTTATGAATCTGAACCGGAGGAACGTACAGCTGAGATGCAGGCTGCG
ATCGCACTCTCCGATACCCTGATCGATGAGCTGTTCGCCGCCGACTGCTATGTGATGGGGATACCCATGTACAACCTAAC
GGTGCCGTCTACGTTTAAAGCGTATCTAGATCAGGTATTTAGACGCGATCGCACCTTGCAAATCGTCAATGGCATCCCCC
AAGGACAACTAGCGAACAAAAAACTTTTAGTGATTACTACCCGAAAATATAATTACCGAGTGGGGTCAGGACGAGAAGAT
CGAGATTTTCTAGAACCATATCTCAGTGCCATTTTTAAGGTTATAGGGCTGACTGATATTTCATATATTCATGCTGACCA
ATTAGCATCAGCCCTTGAACGAGAGCACTCTCTGGCTAAGGCAACCCAAATGATTCATCAACTAGCTTTGAACTTTTAA

Upstream 100 bases:

>100_bases
GAGATTAGTTATCCCACCCAATAGACTTGCACTGTCTCGACATCTTCAGGTTCTATTAACTACAATTCTTAATTTTGGCA
AGAGAAGTAGACTGTTAGGT

Downstream 100 bases:

>100_bases
TGGCTATCTAACAGGAAGGCTGGAAAGCGTCTACTTCCCAGCCTCATTGATTGGTTGAATTCCAGTAAGTTGTACCCGTC
ACCCTGCCAGCAAAATTGCG

Product: FMN-dependent NADH-azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2 [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLSPDSKPRCLLHMDVSARVQGSYSRQLTQKFVSQWQQANPHHQIIYRDIGRFPIPHIDETWVAAYESEPEERTAEMQAA
IALSDTLIDELFAADCYVMGIPMYNLTVPSTFKAYLDQVFRRDRTLQIVNGIPQGQLANKKLLVITTRKYNYRVGSGRED
RDFLEPYLSAIFKVIGLTDISYIHADQLASALEREHSLAKATQMIHQLALNF

Sequences:

>Translated_212_residues
MLSPDSKPRCLLHMDVSARVQGSYSRQLTQKFVSQWQQANPHHQIIYRDIGRFPIPHIDETWVAAYESEPEERTAEMQAA
IALSDTLIDELFAADCYVMGIPMYNLTVPSTFKAYLDQVFRRDRTLQIVNGIPQGQLANKKLLVITTRKYNYRVGSGRED
RDFLEPYLSAIFKVIGLTDISYIHADQLASALEREHSLAKATQMIHQLALNF
>Mature_212_residues
MLSPDSKPRCLLHMDVSARVQGSYSRQLTQKFVSQWQQANPHHQIIYRDIGRFPIPHIDETWVAAYESEPEERTAEMQAA
IALSDTLIDELFAADCYVMGIPMYNLTVPSTFKAYLDQVFRRDRTLQIVNGIPQGQLANKKLLVITTRKYNYRVGSGRED
RDFLEPYLSAIFKVIGLTDISYIHADQLASALEREHSLAKATQMIHQLALNF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=182, Percent_Identity=36.2637362637363, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 24275; Mature: 24275

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPDSKPRCLLHMDVSARVQGSYSRQLTQKFVSQWQQANPHHQIIYRDIGRFPIPHIDE
CCCCCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
TWVAAYESEPEERTAEMQAAIALSDTLIDELFAADCYVMGIPMYNLTVPSTFKAYLDQVF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCHHHHHHHHHHH
RRDRTLQIVNGIPQGQLANKKLLVITTRKYNYRVGSGREDRDFLEPYLSAIFKVIGLTDI
HCCCCEEEECCCCCCCCCCCEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
SYIHADQLASALEREHSLAKATQMIHQLALNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSPDSKPRCLLHMDVSARVQGSYSRQLTQKFVSQWQQANPHHQIIYRDIGRFPIPHIDE
CCCCCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
TWVAAYESEPEERTAEMQAAIALSDTLIDELFAADCYVMGIPMYNLTVPSTFKAYLDQVF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCHHHHHHHHHHH
RRDRTLQIVNGIPQGQLANKKLLVITTRKYNYRVGSGREDRDFLEPYLSAIFKVIGLTDI
HCCCCEEEECCCCCCCCCCCEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
SYIHADQLASALEREHSLAKATQMIHQLALNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA