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The map label for this gene is nemA [H]

Identifier: 158337953

GI number: 158337953

Start: 4880199

End: 4881299

Strand: Direct

Name: nemA [H]

Synonym: AM1_4840

Alternate gene names: 158337953

Gene position: 4880199-4881299 (Clockwise)

Preceding gene: 158337952

Following gene: 158337956

Centisome position: 75.04

GC content: 51.41

Gene sequence:

>1101_bases
ATGTCAATTTCATCCGTGTTCGAACCCATTCGTCTGGGTGCGGTTGACCTCCGCAACCGCATGGTAATGGCACCGTTAAC
CCGAGGTCGATCAGGACCAGATCGAGTTCCCAACGCTTTAATGGCTGAGTATTACCAGCAACGGGCGAGTGCGGGTCTGA
TTATTACGGAAGCGACTCAGGTCTCTGAGCAAGCGGCGGGCTGGTCTGAAACGCCAGGGATCTACTCTGAAGCCCAAATA
CAGGCTTGGCGCCAGGTAACAGATGCCGTCCACCAGCAAGATGGCAAGATTTTCCTGCAAATTTGGCATACAGGACGGGC
CTCTCATCCAGATTTTCAGTTGAATGGGGCACGACCGATTTCTGCCTCGGCGATTAAGCCAGCAGGTGAAGTCCATACTC
CGCAGGGTAAGAAACCATTTGTCACCCCCAGAGCTGTGAGTCTGGACGAGATTCCAAGCATTGTTCAAGATTTTGCTCAG
GCCACTGGCAATGCTCGAAAAGCGGGATTTGATGGGGTTGAAATCCATGGGGCTAATGGATACTTAATTGATCAGTTCCT
TCGAGATGGCACCAACGAACGGCAAGATGCCTATGGGGGCACGATCGAAAACCGTACCCGGTTTCTGTTAGAAGTTGTTG
AGGCAGCGGTAGCTGTCTGGAGTGCTGATCATGTAGGCGTTCGACTTTCTCCTACGAATGCCTTTAATGACATGAGAGAT
AGTAATCCGATCTCAACCTTCACACATGCAGCTCAAGCCCTCAATACTTACAATTTGGCCTATCTTCATGTCCTTGAAGC
CTTGCAAGGGCATATGCTGGCAGTGGAAGGCGAGCGGGTGACTCCCTATATCCGACAGGTTTTTCAAGGTCCGCTGATGA
TCAACGGTGGCTATGATGCAGTCAGTGGTGCGGCTGCGATCGCAAACCAAGAAGCCGATTTAGTCGCCTATGGCGTCCCG
TTTATTGCTAACCCTGACTTACCGGAACGCTTTGCAAAACAAGCCCCCCTCAATGAACCCGACCCATCCACGTTCTATAC
CCGGGGGGCAGAAGGCTATACGGACTATCCATTTTTAGATCCACTAATAACTGCCGCATAG

Upstream 100 bases:

>100_bases
CCTGACGGTAAGGCGAAGCGCGCGCCGCCTTACCGTTTACTTTGTCCTGCTTGGCAGGCAAGTTCAGTTCTTATACTCCA
AACCAACAGGGAGAGTTCCA

Downstream 100 bases:

>100_bases
CATTGGGCTTTTTATCCTGATACTTTCCTAGAACGTCTCGTGGTTTCCCGTTGGGGCACTACGAGGCTTTTTTGATCGGT
GAGTTTGCACCTAAAAATTA

Product: oxidoreductase, FAD/FMN-binding family protein

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSISSVFEPIRLGAVDLRNRMVMAPLTRGRSGPDRVPNALMAEYYQQRASAGLIITEATQVSEQAAGWSETPGIYSEAQI
QAWRQVTDAVHQQDGKIFLQIWHTGRASHPDFQLNGARPISASAIKPAGEVHTPQGKKPFVTPRAVSLDEIPSIVQDFAQ
ATGNARKAGFDGVEIHGANGYLIDQFLRDGTNERQDAYGGTIENRTRFLLEVVEAAVAVWSADHVGVRLSPTNAFNDMRD
SNPISTFTHAAQALNTYNLAYLHVLEALQGHMLAVEGERVTPYIRQVFQGPLMINGGYDAVSGAAAIANQEADLVAYGVP
FIANPDLPERFAKQAPLNEPDPSTFYTRGAEGYTDYPFLDPLITAA

Sequences:

>Translated_366_residues
MSISSVFEPIRLGAVDLRNRMVMAPLTRGRSGPDRVPNALMAEYYQQRASAGLIITEATQVSEQAAGWSETPGIYSEAQI
QAWRQVTDAVHQQDGKIFLQIWHTGRASHPDFQLNGARPISASAIKPAGEVHTPQGKKPFVTPRAVSLDEIPSIVQDFAQ
ATGNARKAGFDGVEIHGANGYLIDQFLRDGTNERQDAYGGTIENRTRFLLEVVEAAVAVWSADHVGVRLSPTNAFNDMRD
SNPISTFTHAAQALNTYNLAYLHVLEALQGHMLAVEGERVTPYIRQVFQGPLMINGGYDAVSGAAAIANQEADLVAYGVP
FIANPDLPERFAKQAPLNEPDPSTFYTRGAEGYTDYPFLDPLITAA
>Mature_365_residues
SISSVFEPIRLGAVDLRNRMVMAPLTRGRSGPDRVPNALMAEYYQQRASAGLIITEATQVSEQAAGWSETPGIYSEAQIQ
AWRQVTDAVHQQDGKIFLQIWHTGRASHPDFQLNGARPISASAIKPAGEVHTPQGKKPFVTPRAVSLDEIPSIVQDFAQA
TGNARKAGFDGVEIHGANGYLIDQFLRDGTNERQDAYGGTIENRTRFLLEVVEAAVAVWSADHVGVRLSPTNAFNDMRDS
NPISTFTHAAQALNTYNLAYLHVLEALQGHMLAVEGERVTPYIRQVFQGPLMINGGYDAVSGAAAIANQEADLVAYGVPF
IANPDLPERFAKQAPLNEPDPSTFYTRGAEGYTDYPFLDPLITAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=370, Percent_Identity=44.0540540540541, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789463, Length=243, Percent_Identity=34.9794238683128, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=353, Percent_Identity=27.7620396600567, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=257, Percent_Identity=29.5719844357977, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=346, Percent_Identity=26.5895953757225, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=352, Percent_Identity=26.1363636363636, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=258, Percent_Identity=28.2945736434109, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=261, Percent_Identity=27.9693486590038, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=375, Percent_Identity=38.1333333333333, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6321973, Length=376, Percent_Identity=37.7659574468085, Blast_Score=224, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39837; Mature: 39706

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISSVFEPIRLGAVDLRNRMVMAPLTRGRSGPDRVPNALMAEYYQQRASAGLIITEATQ
CCHHHHHHHHHCCHHHHHCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECHHH
VSEQAAGWSETPGIYSEAQIQAWRQVTDAVHQQDGKIFLQIWHTGRASHPDFQLNGARPI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEECCCCCC
SASAIKPAGEVHTPQGKKPFVTPRAVSLDEIPSIVQDFAQATGNARKAGFDGVEIHGANG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
YLIDQFLRDGTNERQDAYGGTIENRTRFLLEVVEAAVAVWSADHVGVRLSPTNAFNDMRD
HHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCC
SNPISTFTHAAQALNTYNLAYLHVLEALQGHMLAVEGERVTPYIRQVFQGPLMINGGYDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCEEEECCCCH
VSGAAAIANQEADLVAYGVPFIANPDLPERFAKQAPLNEPDPSTFYTRGAEGYTDYPFLD
HHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHH
PLITAA
HHHHCC
>Mature Secondary Structure 
SISSVFEPIRLGAVDLRNRMVMAPLTRGRSGPDRVPNALMAEYYQQRASAGLIITEATQ
CHHHHHHHHHCCHHHHHCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECHHH
VSEQAAGWSETPGIYSEAQIQAWRQVTDAVHQQDGKIFLQIWHTGRASHPDFQLNGARPI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEECCCCCC
SASAIKPAGEVHTPQGKKPFVTPRAVSLDEIPSIVQDFAQATGNARKAGFDGVEIHGANG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
YLIDQFLRDGTNERQDAYGGTIENRTRFLLEVVEAAVAVWSADHVGVRLSPTNAFNDMRD
HHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCC
SNPISTFTHAAQALNTYNLAYLHVLEALQGHMLAVEGERVTPYIRQVFQGPLMINGGYDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCEEEECCCCH
VSGAAAIANQEADLVAYGVPFIANPDLPERFAKQAPLNEPDPSTFYTRGAEGYTDYPFLD
HHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHH
PLITAA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]