Click here to switch to the map view.

The map label for this gene is purK [H]

Identifier: 158337875

GI number: 158337875

Start: 4802333

End: 4803526

Strand: Reverse

Name: purK [H]

Synonym: AM1_4761

Alternate gene names: 158337875

Gene position: 4803526-4802333 (Counterclockwise)

Preceding gene: 158337876

Following gene: 158337874

Centisome position: 73.86

GC content: 51.76

Gene sequence:

>1194_bases
ATGTCTGATTTTGCGATCGCAACTTCGTCCGCTCCTACATCCCCTCAAGTCCCCATTCATACCGTTGGTGTTCTGGGCGG
TGGTCAGCTAGCTTGGATGTTAAATCAAGCAGCTCCATCCTTAGACCTCAACCTCGTTTTCCAAACCCCCAACGTCTCTG
ACTCAGCCCTCCAAAGTGTGGCGGATCTAGACCAGCAGGCCATTCTCGCCCCTATTACGGATGCTGCCGCCACCGCTCAA
CTCGCTCAACAGTGCCAAGTCATTACCTTCGAAAATGAGTTTGTTGATTTAGACGCCTTAGGAAAGCTAGAAGCCCAAGG
GGCTTGCTTTCGCCCCAGCTTAGCCAGCCTTACGCCCCTACTCGATAAGTATGAGCAACGCAGCTTTTTTGCTTCCCTTG
GCATATCTGTCCCCCAATTTGCAACCCTAACATCTGACTCCCTGCCCCCATCCTTCGAGTTTCCCGTCGTTGTTAAAGCG
CGGCGCAATGGCTATGACGGCCAGGGCACTTTTATTTGTCATACTTCGGCAGACTTGGCCCAATGTTGGCAGCAAGCCAA
ACAGGATCCGTCTGTATTGATGGTGGAAGCTTATGTCCCCTTTCAGTGGGAGCTGGCGGTTATGGCAGCTCGCTCTACCA
CGGGGGAGATTTCCCTGTTCCCAGTGGTAGAAACTCATCAGCAGGATCAGGTTTGTCATTGGGCTATTGCCCCTGTGCAA
CTATCGCCCCAAACTCAGGCACAAATTGATCAGATCGCATCCCAGATATTAGAACATCTGGATATGGTGGGGATTGTGGG
GATTGAGTTATTCCAAACCGCTGACGACCAAGTGTGGGTCAATGAAATTGCCCCTCGCACCCATAATTCTGGCCATCTCA
CCATCGATGCCTGCACCACCTCCCAATTTGCGCAGCATCTACGGGCGATTAGTGGTCAGCCTTTAGGGCCAACTCACCTC
ACTTCCGCTGCCGTCATGGTCAACCTACTGGGATTTGAAACCAGCCACCATGATTATTTACAAAAACGGCAGGCCATTGC
TGCCCTTCCCCAGACCTTCGTTCATTGGTATGGCAAGACAGAAAGTCGGCCTGGGCGGAAGCTAGGACATGCGACAATGT
TATTATCCTCTGAAGATTCAGTTATGGCCGTAGAGATGGCCCATAAAATTGAAGCCCTTTGGTATGGGGGCTGA

Upstream 100 bases:

>100_bases
GGTGTCCATGGGCAATACTGAACGGGTTCAGGGCGATGTCGCTTCGGAAACACCACCAGAAGCAGAGCCTGAGCCAGCCT
TTTAAAAATAACTAGGTTTT

Downstream 100 bases:

>100_bases
GGTGTAGATAACGTTATGAGCAGTAAGGCTGCCCGAGCTTATATCCTGTTATCGATTGCGGCTGCATTGATAACAATGGT
GCTGAAATTTGGTGCCTATC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSDFAIATSSAPTSPQVPIHTVGVLGGGQLAWMLNQAAPSLDLNLVFQTPNVSDSALQSVADLDQQAILAPITDAAATAQ
LAQQCQVITFENEFVDLDALGKLEAQGACFRPSLASLTPLLDKYEQRSFFASLGISVPQFATLTSDSLPPSFEFPVVVKA
RRNGYDGQGTFICHTSADLAQCWQQAKQDPSVLMVEAYVPFQWELAVMAARSTTGEISLFPVVETHQQDQVCHWAIAPVQ
LSPQTQAQIDQIASQILEHLDMVGIVGIELFQTADDQVWVNEIAPRTHNSGHLTIDACTTSQFAQHLRAISGQPLGPTHL
TSAAVMVNLLGFETSHHDYLQKRQAIAALPQTFVHWYGKTESRPGRKLGHATMLLSSEDSVMAVEMAHKIEALWYGG

Sequences:

>Translated_397_residues
MSDFAIATSSAPTSPQVPIHTVGVLGGGQLAWMLNQAAPSLDLNLVFQTPNVSDSALQSVADLDQQAILAPITDAAATAQ
LAQQCQVITFENEFVDLDALGKLEAQGACFRPSLASLTPLLDKYEQRSFFASLGISVPQFATLTSDSLPPSFEFPVVVKA
RRNGYDGQGTFICHTSADLAQCWQQAKQDPSVLMVEAYVPFQWELAVMAARSTTGEISLFPVVETHQQDQVCHWAIAPVQ
LSPQTQAQIDQIASQILEHLDMVGIVGIELFQTADDQVWVNEIAPRTHNSGHLTIDACTTSQFAQHLRAISGQPLGPTHL
TSAAVMVNLLGFETSHHDYLQKRQAIAALPQTFVHWYGKTESRPGRKLGHATMLLSSEDSVMAVEMAHKIEALWYGG
>Mature_396_residues
SDFAIATSSAPTSPQVPIHTVGVLGGGQLAWMLNQAAPSLDLNLVFQTPNVSDSALQSVADLDQQAILAPITDAAATAQL
AQQCQVITFENEFVDLDALGKLEAQGACFRPSLASLTPLLDKYEQRSFFASLGISVPQFATLTSDSLPPSFEFPVVVKAR
RNGYDGQGTFICHTSADLAQCWQQAKQDPSVLMVEAYVPFQWELAVMAARSTTGEISLFPVVETHQQDQVCHWAIAPVQL
SPQTQAQIDQIASQILEHLDMVGIVGIELFQTADDQVWVNEIAPRTHNSGHLTIDACTTSQFAQHLRAISGQPLGPTHLT
SAAVMVNLLGFETSHHDYLQKRQAIAALPQTFVHWYGKTESRPGRKLGHATMLLSSEDSVMAVEMAHKIEALWYGG

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=375, Percent_Identity=30.4, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788155, Length=365, Percent_Identity=26.5753424657534, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324702, Length=372, Percent_Identity=33.8709677419355, Blast_Score=211, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 43088; Mature: 42957

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFAIATSSAPTSPQVPIHTVGVLGGGQLAWMLNQAAPSLDLNLVFQTPNVSDSALQSV
CCCCEEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHH
ADLDQQAILAPITDAAATAQLAQQCQVITFENEFVDLDALGKLEAQGACFRPSLASLTPL
HHCCHHHEEEECHHHHHHHHHHHCCEEEEECCCEECHHHCCCCCCCCCCCCCCHHHHHHH
LDKYEQRSFFASLGISVPQFATLTSDSLPPSFEFPVVVKARRNGYDGQGTFICHTSADLA
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCHHHH
QCWQQAKQDPSVLMVEAYVPFQWELAVMAARSTTGEISLFPVVETHQQDQVCHWAIAPVQ
HHHHHHCCCCCEEEEEEECCCEEEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECEE
LSPQTQAQIDQIASQILEHLDMVGIVGIELFQTADDQVWVNEIAPRTHNSGHLTIDACTT
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEEECCH
SQFAQHLRAISGQPLGPTHLTSAAVMVNLLGFETSHHDYLQKRQAIAALPQTFVHWYGKT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ESRPGRKLGHATMLLSSEDSVMAVEMAHKIEALWYGG
CCCCCCHHCCEEEEEECCCCEEEEEHHHHHEEEEECC
>Mature Secondary Structure 
SDFAIATSSAPTSPQVPIHTVGVLGGGQLAWMLNQAAPSLDLNLVFQTPNVSDSALQSV
CCCEEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHH
ADLDQQAILAPITDAAATAQLAQQCQVITFENEFVDLDALGKLEAQGACFRPSLASLTPL
HHCCHHHEEEECHHHHHHHHHHHCCEEEEECCCEECHHHCCCCCCCCCCCCCCHHHHHHH
LDKYEQRSFFASLGISVPQFATLTSDSLPPSFEFPVVVKARRNGYDGQGTFICHTSADLA
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCHHHH
QCWQQAKQDPSVLMVEAYVPFQWELAVMAARSTTGEISLFPVVETHQQDQVCHWAIAPVQ
HHHHHHCCCCCEEEEEEECCCEEEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECEE
LSPQTQAQIDQIASQILEHLDMVGIVGIELFQTADDQVWVNEIAPRTHNSGHLTIDACTT
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEEECCH
SQFAQHLRAISGQPLGPTHLTSAAVMVNLLGFETSHHDYLQKRQAIAALPQTFVHWYGKT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ESRPGRKLGHATMLLSSEDSVMAVEMAHKIEALWYGG
CCCCCCHHCCEEEEEECCCCEEEEEHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]