Click here to switch to the map view.

The map label for this gene is fliA [H]

Identifier: 158337871

GI number: 158337871

Start: 4798718

End: 4799515

Strand: Reverse

Name: fliA [H]

Synonym: AM1_4757

Alternate gene names: 158337871

Gene position: 4799515-4798718 (Counterclockwise)

Preceding gene: 158337874

Following gene: 158337870

Centisome position: 73.8

GC content: 48.75

Gene sequence:

>798_bases
ATGGCTTATCAAACTTCCTTACAGTCCCAAACAATGGAGATGCTGGTTGCTTATCGCCAGAAACCTTCTGTTCAGCTCCG
AAACCGTTTGGTCCGCTTGAATATGGGATTAGTCCGCAAGGTGGCTCACCGTCTCGCTCATCAATGTGCAGAGCCTTATG
AAGATTTAGAACAATGTGGTTTCCTGGGATTGATCACCGCTATCGAGCGCTTTGATCCGACCCAAGGTTATGCCTTTAGC
TCATTTGCTGTTCCCTACATTAGAGGCGAAATCCTCCACTTCTTGCGGGATCGTGCCAATACTGTTCGCATCCCTCGTCG
ATGGCAACAGCTGAGTCGCGATGGCGCAAAAGTTCGCCAGGCATTAACGATGGAACTAGGACGCCATCCCAGCGATCAAG
AAATCGCCGACGAACTCGATCTATCTATGCAAGAATGGCGAGCAGTTAAGCTAGCAGCATCTAACCGCGTTCCTCTTAGC
TTGAATGCCCGCGTCTCTTCCGGCCATCATCAGCAGTCTGATTCCATGATGACCCTTGGGGATACTCTGATGGATGCTCG
GTGCCAGATTATGCAAACGAATCAAGAAGATCGCTTAGAACTTCAGCAAGCACTGGATCAACTAGAAGATCGCACCCGAG
AAATGATCGAGTCTGTCTTTGTCCATCAACTGTCTCGACAAGAAGTGGCTCAACGGATTGGCGTTAGCCCCGTTACCGTT
ACTCGACGAATCAAGAAAGGAATTGACGAGTTAGTCGGTCTCTTGCAAAAATCTGCTGCATTACAAATGGAAGTCTAA

Upstream 100 bases:

>100_bases
TTCCATTTATTTTTATAAAGTCCCGTATTTTAACGATGGTTTTTCAAGTTCAGTATCTCGATAATGATTTCCAGGTACAG
GACATCAGGACGATTAAACT

Downstream 100 bases:

>100_bases
TTGATTTCGGTAGCTCTAAATGGCTGAGCCATTTACCTTAACTAAGGTTTAAACCATCCCAGGATAAGTGTGCGCTGAGA
TGATAGATAGCAGGGTAACT

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MAYQTSLQSQTMEMLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLAHQCAEPYEDLEQCGFLGLITAIERFDPTQGYAFS
SFAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDGAKVRQALTMELGRHPSDQEIADELDLSMQEWRAVKLAASNRVPLS
LNARVSSGHHQQSDSMMTLGDTLMDARCQIMQTNQEDRLELQQALDQLEDRTREMIESVFVHQLSRQEVAQRIGVSPVTV
TRRIKKGIDELVGLLQKSAALQMEV

Sequences:

>Translated_265_residues
MAYQTSLQSQTMEMLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLAHQCAEPYEDLEQCGFLGLITAIERFDPTQGYAFS
SFAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDGAKVRQALTMELGRHPSDQEIADELDLSMQEWRAVKLAASNRVPLS
LNARVSSGHHQQSDSMMTLGDTLMDARCQIMQTNQEDRLELQQALDQLEDRTREMIESVFVHQLSRQEVAQRIGVSPVTV
TRRIKKGIDELVGLLQKSAALQMEV
>Mature_264_residues
AYQTSLQSQTMEMLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLAHQCAEPYEDLEQCGFLGLITAIERFDPTQGYAFSS
FAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDGAKVRQALTMELGRHPSDQEIADELDLSMQEWRAVKLAASNRVPLSL
NARVSSGHHQQSDSMMTLGDTLMDARCQIMQTNQEDRLELQQALDQLEDRTREMIESVFVHQLSRQEVAQRIGVSPVTVT
RRIKKGIDELVGLLQKSAALQMEV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=28.110599078341, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789448, Length=165, Percent_Identity=26.0606060606061, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789098, Length=224, Percent_Identity=27.6785714285714, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30370; Mature: 30239

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQTSLQSQTMEMLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLAHQCAEPYEDLEQCG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
FLGLITAIERFDPTQGYAFSSFAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDGAKVRQ
HHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHH
ALTMELGRHPSDQEIADELDLSMQEWRAVKLAASNRVPLSLNARVSSGHHQQSDSMMTLG
HHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHH
DTLMDARCQIMQTNQEDRLELQQALDQLEDRTREMIESVFVHQLSRQEVAQRIGVSPVTV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TRRIKKGIDELVGLLQKSAALQMEV
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AYQTSLQSQTMEMLVAYRQKPSVQLRNRLVRLNMGLVRKVAHRLAHQCAEPYEDLEQCG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
FLGLITAIERFDPTQGYAFSSFAVPYIRGEILHFLRDRANTVRIPRRWQQLSRDGAKVRQ
HHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHH
ALTMELGRHPSDQEIADELDLSMQEWRAVKLAASNRVPLSLNARVSSGHHQQSDSMMTLG
HHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHH
DTLMDARCQIMQTNQEDRLELQQALDQLEDRTREMIESVFVHQLSRQEVAQRIGVSPVTV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TRRIKKGIDELVGLLQKSAALQMEV
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]