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The map label for this gene is sseA [C]

Identifier: 158337845

GI number: 158337845

Start: 4763456

End: 4764289

Strand: Direct

Name: sseA [C]

Synonym: AM1_4731

Alternate gene names: 158337845

Gene position: 4763456-4764289 (Clockwise)

Preceding gene: 158337841

Following gene: 158337850

Centisome position: 73.24

GC content: 48.68

Gene sequence:

>834_bases
ATGTCTCAATACGCCCATCCCGAAGTCTTAGTGGATACCCAATGGCTGATGGAACATCTCAATGATTCCAATCTCCGCAT
TCTTGAAGTGGATATGAATCCAGAGGCTTATAACGATGCCCATATTCCAGGTGCCGTCTTTTGGCCGATCGCTGAACTGA
TGAGGCCAGACTTGTCCCTCAATTTAGATCCAGTCGCCTTTAGCGCATTGCTGGGCAAGTCTGGGATTAATGCCCATACA
ACCGTTGTTGCTTATGGCAGCTACCCTGGCACGGGGGCCTTTCTATTTTGGCTATTTCGGCTCTTTGGTCATCCAAATAT
TGTGGTGCTTAACGGGGGATATCAGCAATGGATCGCTCAAGGAGGAGCGGTAGTTTCAGAGCTTTCTACTTTCCCAGCTA
CGGAGTATCGGGTGCAAGGACCGGATGCTAATTTACGAGTTTTGTCTGCCGAAGTATTGGCTGCGCTAGAGAGACCCGAT
CAAATCCTGCTAGATGTGAGAACAGAGGCTGAATATACGGGTGAGTTATACCTGATGGAGCCACCCCAAGGGGATGAAGT
GGGAGGCCATATTCCGGGTGCAGTGCATCTGGACCATGCCGAAACGTTGAATGAGGATGGCACATTCAAGTCAGCGGATG
AGTTACATACACTTTTTCAGCAGCAAGGGATTACACCCGAAAAGGAGATATTTCCCTACTGTGCCATTGGTGGGCGATCT
GCCTATATTTGGTTTGTTTTGACCTATCTGTTGGGCTATCCCCAAGTTCGGAATTATGATGGCTCTTGGCAAGAGTGGAG
TCGATTACCCAACGTTCCCATTGATCAAGGATGA

Upstream 100 bases:

>100_bases
TGATCATCACAATGCCTCAAAGAGTACCCGATCGAGGACTGGGTCTGGGTGGCCTACTTCCCTAATCTTAGCGGTAGTTC
TTTTCACGGGAGTACCGTCC

Downstream 100 bases:

>100_bases
AAGTACTTTGGGAGTTTGAATGGCTAGCATTGTTTCAATCAGCTCTATATCGATCTGGGACGATACCAACCATTCGTTGG
TTATCCAAGAAAGGGGAGTA

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSQYAHPEVLVDTQWLMEHLNDSNLRILEVDMNPEAYNDAHIPGAVFWPIAELMRPDLSLNLDPVAFSALLGKSGINAHT
TVVAYGSYPGTGAFLFWLFRLFGHPNIVVLNGGYQQWIAQGGAVVSELSTFPATEYRVQGPDANLRVLSAEVLAALERPD
QILLDVRTEAEYTGELYLMEPPQGDEVGGHIPGAVHLDHAETLNEDGTFKSADELHTLFQQQGITPEKEIFPYCAIGGRS
AYIWFVLTYLLGYPQVRNYDGSWQEWSRLPNVPIDQG

Sequences:

>Translated_277_residues
MSQYAHPEVLVDTQWLMEHLNDSNLRILEVDMNPEAYNDAHIPGAVFWPIAELMRPDLSLNLDPVAFSALLGKSGINAHT
TVVAYGSYPGTGAFLFWLFRLFGHPNIVVLNGGYQQWIAQGGAVVSELSTFPATEYRVQGPDANLRVLSAEVLAALERPD
QILLDVRTEAEYTGELYLMEPPQGDEVGGHIPGAVHLDHAETLNEDGTFKSADELHTLFQQQGITPEKEIFPYCAIGGRS
AYIWFVLTYLLGYPQVRNYDGSWQEWSRLPNVPIDQG
>Mature_276_residues
SQYAHPEVLVDTQWLMEHLNDSNLRILEVDMNPEAYNDAHIPGAVFWPIAELMRPDLSLNLDPVAFSALLGKSGINAHTT
VVAYGSYPGTGAFLFWLFRLFGHPNIVVLNGGYQQWIAQGGAVVSELSTFPATEYRVQGPDANLRVLSAEVLAALERPDQ
ILLDVRTEAEYTGELYLMEPPQGDEVGGHIPGAVHLDHAETLNEDGTFKSADELHTLFQQQGITPEKEIFPYCAIGGRSA
YIWFVLTYLLGYPQVRNYDGSWQEWSRLPNVPIDQG

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=292, Percent_Identity=32.5342465753425, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI61835204, Length=270, Percent_Identity=30, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI194473681, Length=270, Percent_Identity=30, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI194473668, Length=270, Percent_Identity=30.3703703703704, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI87082121, Length=280, Percent_Identity=28.2142857142857, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081967, Length=296, Percent_Identity=29.0540540540541, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17561888, Length=304, Percent_Identity=25.3289473684211, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71997283, Length=305, Percent_Identity=25.2459016393443, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115534702, Length=211, Percent_Identity=27.4881516587678, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 30844; Mature: 30713

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYAHPEVLVDTQWLMEHLNDSNLRILEVDMNPEAYNDAHIPGAVFWPIAELMRPDLSL
CCCCCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCC
NLDPVAFSALLGKSGINAHTTVVAYGSYPGTGAFLFWLFRLFGHPNIVVLNGGYQQWIAQ
CCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHC
GGAVVSELSTFPATEYRVQGPDANLRVLSAEVLAALERPDQILLDVRTEAEYTGELYLME
CCHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEC
PPQGDEVGGHIPGAVHLDHAETLNEDGTFKSADELHTLFQQQGITPEKEIFPYCAIGGRS
CCCCCCCCCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCEEEECCCC
AYIWFVLTYLLGYPQVRNYDGSWQEWSRLPNVPIDQG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQYAHPEVLVDTQWLMEHLNDSNLRILEVDMNPEAYNDAHIPGAVFWPIAELMRPDLSL
CCCCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCC
NLDPVAFSALLGKSGINAHTTVVAYGSYPGTGAFLFWLFRLFGHPNIVVLNGGYQQWIAQ
CCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHC
GGAVVSELSTFPATEYRVQGPDANLRVLSAEVLAALERPDQILLDVRTEAEYTGELYLME
CCHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEC
PPQGDEVGGHIPGAVHLDHAETLNEDGTFKSADELHTLFQQQGITPEKEIFPYCAIGGRS
CCCCCCCCCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCEEEECCCC
AYIWFVLTYLLGYPQVRNYDGSWQEWSRLPNVPIDQG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]