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The map label for this gene is cbiH [H]

Identifier: 158337802

GI number: 158337802

Start: 4721427

End: 4723253

Strand: Reverse

Name: cbiH [H]

Synonym: AM1_4688

Alternate gene names: 158337802

Gene position: 4723253-4721427 (Counterclockwise)

Preceding gene: 158337833

Following gene: 158337799

Centisome position: 72.62

GC content: 55.12

Gene sequence:

>1827_bases
TTGTTTCAAACCTTCCAGCCTTTAGCCGCCATCGCCACCACCCCCAACGCCGTCCACACCCTCCATCCCCTCTGTCAACA
AACCGGAGCCACCCTTTGGGTCCCTGCCTCCCTCTCCCACCTACCTCACACCCAAGCCTATAGCGGCTCCCTCAAAGACC
ATCTCCAAACCTTGTGGAACGACCATCAGGGTCTGATCTGTTGCCTTGCAACCGGAGCCGTCGTCCGCCTAATCGCCCCC
CTCCTAACCGACAAATCCCAAGATCCTGCCGTTGTCGTCCTTGATGAACCCGGCCAATTCGTGCTCAGCCTCTGCAGTGG
TCACCTGGGGAGAGCGGATCAACTTACCCAACTCCTCGCCCAACAAATCGGGGCCACCCCTATCCTCACCGGGGCCAGTA
ATGCCGCTAACTTGCCTAGCATTGATACCCTCGGCATCCCCTTCGGCTGGCAACGAGGGGCTGGTGATTGGAACGCTATC
GCTGGAGCCATTGCCCGAGGTGAAACTGTCACTGTCCACCAAGCCGCTGGTTCCGAACTATGGCAAGCTCATTTACCCCC
ATCTCACACCTATCAATTCGTTAGCGATACTGAAGCGCCCCAAATTTGGATCAGCCCCAAAGTCCTATCCCCAAAACAAT
CCAGCGTCCAATGGCATCCCCGCGTTCTCTGGGTTGGCATGGGCTGCGAGCGAGGCACCTCCCGACAGCTAATGGAACAG
GCCCTGCGACAAGTCTGTGAACAACATCAACTGGCTTGGGAAGCCATTGCTGGGGTTGCCACCCTAGACCTGAAAGCCGA
TGAAGTCGGGTTAGTGGATCTCTGCCAACAGGCTAACTGGCCTCTAAAATGCTACTCCCCTGAAGACTTAAAAGCCGTAG
CAGTCCCCACCCCTTCCGATGTCGTTAAGGCTGAAGTGGGCACCCCCAGCGTGGCTGAAGCCGCTGCCCTTAAAGCTGCC
CAGCAATTCACCCTCCTGGCAACCAAGCAAATCTTCAAAGACCCGGCACAACCTGGAGCGGTCACCATTGCCATTGCTCA
AGCTGAACAAGAATATACCGGGCGTAGGGGGCAACTGCTTCTAGTAGGAACGGGACCTGGTGACCTGGACCAAATGACTC
CTGCATCCAAAACGGCCATTACCCAAGCAGATGTGGTGATTGGCTATACCCTCTATGTGGACTTGGTGCGATCGCAGTTT
CGTCCTGGTCAAATCATTGAACCGTCCCCCATTACCCAAGAACGCCAACGCGCCCAACGGGCCATCGATTTAGCTCAGTG
GGGCCTGACTGTTGCCGTGATTTCTTCGGGTGATGCTGGTATTTATGGCATGGCCGGTTTAGTGATGGAAGCACTACAGC
AGAATGGTTGGGATGGGCAGACCCCAGTCGTCCAAATTTTTCCCGGAATTTCTGCTCTGCAAGCAGCTGCTTCTCAAGTG
GGTACGCCCCTTATGCATGACTTTTGCGCCATTAGCCTCAGTGATTTGCTCACCCCTTGGTCCGTGATTGAAAAGCGCCT
AGAGGCTGCCGCTGCTGCTGATTTTGTCATGGCTCTGTACAATCCGCGATCGCAAAAACGCATCGAACATCTACTCAAAG
CCCAGCAAATCTGTTTGCAACATCGAGATCCACAAACCCCCGTAGCCGTGGTGAGATCGGTCTATCGTCCAGACCAAACC
ACTCATCTGGGAACGCTATCAGAGCTGGATACGCTGCCCGTAGATATGCTCAGCACCGTGATTATCGGCAATGCCACCAC
TCGGTTTCACCAGGATTGGATGATTACCCCCAGAGGGTATTTCCCTCCTTCTAATCCCTCGACTTAG

Upstream 100 bases:

>100_bases
TCGGCCATTCTGAAACTGCGTTAAGTAAACAGTTTTCTCTATTGGCCATAGTAGACTTCTCCTATACTTCCACCTACAGG
CATTCCACTTGAGTCAACGT

Downstream 100 bases:

>100_bases
CTCAGATCGAACTAGCTAGGTCTAAGAGACCTTTGCAAACCACCGCCGAATCAACTCCACCTGAAAGCAATACCCTCCAT
CTACAGATTGAATGAGGTCT

Product: bifunctional cbiG protein and precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MFQTFQPLAAIATTPNAVHTLHPLCQQTGATLWVPASLSHLPHTQAYSGSLKDHLQTLWNDHQGLICCLATGAVVRLIAP
LLTDKSQDPAVVVLDEPGQFVLSLCSGHLGRADQLTQLLAQQIGATPILTGASNAANLPSIDTLGIPFGWQRGAGDWNAI
AGAIARGETVTVHQAAGSELWQAHLPPSHTYQFVSDTEAPQIWISPKVLSPKQSSVQWHPRVLWVGMGCERGTSRQLMEQ
ALRQVCEQHQLAWEAIAGVATLDLKADEVGLVDLCQQANWPLKCYSPEDLKAVAVPTPSDVVKAEVGTPSVAEAAALKAA
QQFTLLATKQIFKDPAQPGAVTIAIAQAEQEYTGRRGQLLLVGTGPGDLDQMTPASKTAITQADVVIGYTLYVDLVRSQF
RPGQIIEPSPITQERQRAQRAIDLAQWGLTVAVISSGDAGIYGMAGLVMEALQQNGWDGQTPVVQIFPGISALQAAASQV
GTPLMHDFCAISLSDLLTPWSVIEKRLEAAAAADFVMALYNPRSQKRIEHLLKAQQICLQHRDPQTPVAVVRSVYRPDQT
THLGTLSELDTLPVDMLSTVIIGNATTRFHQDWMITPRGYFPPSNPST

Sequences:

>Translated_608_residues
MFQTFQPLAAIATTPNAVHTLHPLCQQTGATLWVPASLSHLPHTQAYSGSLKDHLQTLWNDHQGLICCLATGAVVRLIAP
LLTDKSQDPAVVVLDEPGQFVLSLCSGHLGRADQLTQLLAQQIGATPILTGASNAANLPSIDTLGIPFGWQRGAGDWNAI
AGAIARGETVTVHQAAGSELWQAHLPPSHTYQFVSDTEAPQIWISPKVLSPKQSSVQWHPRVLWVGMGCERGTSRQLMEQ
ALRQVCEQHQLAWEAIAGVATLDLKADEVGLVDLCQQANWPLKCYSPEDLKAVAVPTPSDVVKAEVGTPSVAEAAALKAA
QQFTLLATKQIFKDPAQPGAVTIAIAQAEQEYTGRRGQLLLVGTGPGDLDQMTPASKTAITQADVVIGYTLYVDLVRSQF
RPGQIIEPSPITQERQRAQRAIDLAQWGLTVAVISSGDAGIYGMAGLVMEALQQNGWDGQTPVVQIFPGISALQAAASQV
GTPLMHDFCAISLSDLLTPWSVIEKRLEAAAAADFVMALYNPRSQKRIEHLLKAQQICLQHRDPQTPVAVVRSVYRPDQT
THLGTLSELDTLPVDMLSTVIIGNATTRFHQDWMITPRGYFPPSNPST
>Mature_608_residues
MFQTFQPLAAIATTPNAVHTLHPLCQQTGATLWVPASLSHLPHTQAYSGSLKDHLQTLWNDHQGLICCLATGAVVRLIAP
LLTDKSQDPAVVVLDEPGQFVLSLCSGHLGRADQLTQLLAQQIGATPILTGASNAANLPSIDTLGIPFGWQRGAGDWNAI
AGAIARGETVTVHQAAGSELWQAHLPPSHTYQFVSDTEAPQIWISPKVLSPKQSSVQWHPRVLWVGMGCERGTSRQLMEQ
ALRQVCEQHQLAWEAIAGVATLDLKADEVGLVDLCQQANWPLKCYSPEDLKAVAVPTPSDVVKAEVGTPSVAEAAALKAA
QQFTLLATKQIFKDPAQPGAVTIAIAQAEQEYTGRRGQLLLVGTGPGDLDQMTPASKTAITQADVVIGYTLYVDLVRSQF
RPGQIIEPSPITQERQRAQRAIDLAQWGLTVAVISSGDAGIYGMAGLVMEALQQNGWDGQTPVVQIFPGISALQAAASQV
GTPLMHDFCAISLSDLLTPWSVIEKRLEAAAAADFVMALYNPRSQKRIEHLLKAQQICLQHRDPQTPVAVVRSVYRPDQT
THLGTLSELDTLPVDMLSTVIIGNATTRFHQDWMITPRGYFPPSNPST

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 65528; Mature: 65528

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQTFQPLAAIATTPNAVHTLHPLCQQTGATLWVPASLSHLPHTQAYSGSLKDHLQTLWN
CCCCCCCHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHCCCCHHHHHHHHHC
DHQGLICCLATGAVVRLIAPLLTDKSQDPAVVVLDEPGQFVLSLCSGHLGRADQLTQLLA
CCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHH
QQIGATPILTGASNAANLPSIDTLGIPFGWQRGAGDWNAIAGAIARGETVTVHQAAGSEL
HHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHH
WQAHLPPSHTYQFVSDTEAPQIWISPKVLSPKQSSVQWHPRVLWVGMGCERGTSRQLMEQ
HCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
ALRQVCEQHQLAWEAIAGVATLDLKADEVGLVDLCQQANWPLKCYSPEDLKAVAVPTPSD
HHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHCCCCEEECCCCCCEEEECCCCHH
VVKAEVGTPSVAEAAALKAAQQFTLLATKQIFKDPAQPGAVTIAIAQAEQEYTGRRGQLL
HHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHCCCCCCEE
LVGTGPGDLDQMTPASKTAITQADVVIGYTLYVDLVRSQFRPGQIIEPSPITQERQRAQR
EEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
AIDLAQWGLTVAVISSGDAGIYGMAGLVMEALQQNGWDGQTPVVQIFPGISALQAAASQV
HHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHH
GTPLMHDFCAISLSDLLTPWSVIEKRLEAAAAADFVMALYNPRSQKRIEHLLKAQQICLQ
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HRDPQTPVAVVRSVYRPDQTTHLGTLSELDTLPVDMLSTVIIGNATTRFHQDWMITPRGY
CCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCHHEECCCEEECCCCC
FPPSNPST
CCCCCCCC
>Mature Secondary Structure
MFQTFQPLAAIATTPNAVHTLHPLCQQTGATLWVPASLSHLPHTQAYSGSLKDHLQTLWN
CCCCCCCHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHCCCCHHHHHHHHHC
DHQGLICCLATGAVVRLIAPLLTDKSQDPAVVVLDEPGQFVLSLCSGHLGRADQLTQLLA
CCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHH
QQIGATPILTGASNAANLPSIDTLGIPFGWQRGAGDWNAIAGAIARGETVTVHQAAGSEL
HHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHH
WQAHLPPSHTYQFVSDTEAPQIWISPKVLSPKQSSVQWHPRVLWVGMGCERGTSRQLMEQ
HCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
ALRQVCEQHQLAWEAIAGVATLDLKADEVGLVDLCQQANWPLKCYSPEDLKAVAVPTPSD
HHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHCCCCEEECCCCCCEEEECCCCHH
VVKAEVGTPSVAEAAALKAAQQFTLLATKQIFKDPAQPGAVTIAIAQAEQEYTGRRGQLL
HHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHCCCCCCEE
LVGTGPGDLDQMTPASKTAITQADVVIGYTLYVDLVRSQFRPGQIIEPSPITQERQRAQR
EEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
AIDLAQWGLTVAVISSGDAGIYGMAGLVMEALQQNGWDGQTPVVQIFPGISALQAAASQV
HHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHH
GTPLMHDFCAISLSDLLTPWSVIEKRLEAAAAADFVMALYNPRSQKRIEHLLKAQQICLQ
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HRDPQTPVAVVRSVYRPDQTTHLGTLSELDTLPVDMLSTVIIGNATTRFHQDWMITPRGY
CCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCHHEECCCEEECCCCC
FPPSNPST
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]