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The map label for this gene is proB

Identifier: 158337792

GI number: 158337792

Start: 4708710

End: 4709825

Strand: Reverse

Name: proB

Synonym: AM1_4676

Alternate gene names: 158337792

Gene position: 4709825-4708710 (Counterclockwise)

Preceding gene: 158337793

Following gene: 158337791

Centisome position: 72.42

GC content: 51.7

Gene sequence:

>1116_bases
ATGTCGCAAACCTTAGTTCTTAAAATTGGCACTTCCAGCTTAACCAACCCAGACACTGGGCATTTGGCTTTAGCAACCAT
TGCCTCCCTTGTGGAAGTCCTTAGCCAACTGCGACAAGAAGGTAACCAAGTGATTCTTGTCTCCTCTGGAGCCGTCGGAA
TTGGCTGTGCCCGTTTGGGTTTAGCCGAACGACCTAAAGCCATTGCCCTTAAGCAAGCCGTTGCAGCAGTCGGTCAAGGG
CGATTGATGCGCATCTACGATGATTTTTTTACTAGTTTGCAGCAGCCTATTGCCCAGGTCCTGCTGACCCGTGGTGACTT
AGCGGATCGCAGTCGGTATATCAACGCCTCCAATACCTTGCAAGAGCTGCTCAAATTAGGTGTGATACCGATTGTGAATG
AAAACGATACGGTAGCCGTAGATGAGCTGCTCCGGTTTGGCGACAACGATACCCTATCGGCCCTAGTGGCAGGATTAGTT
CAGGCGGACTGGCTTTTTATTCTCACAGATGTAGATCGCCTCTATTCGGCGGATCCGCGTATCCAGCCCGATGCCCAGCC
CATTGTCACCGTCGAACGGATGGAGGAATTGGCAGCACTGAATGTCAATGCAGGTGCCCAAGGGTCCCAGTGGGGAACGG
GTGGGATGGCTACCAAAATTTCTGCAGCTGCGATCGCAACCAATGCCGGTGTTCGTACCGTGATTACCCAAGGCAAAACC
CCTGAGAATATTCCTCGCATCTTAGCGGGAGAATCCCTCGGCACCCAATTTCAACCCCATCCTCGCCCCCACAATGCCCG
CAAACATTGGATCGCTCATGCCTTAGTACCGACGGGCAAACTCATTTTGGACGATGGCGCGGCAAAAGCCATTACAACGC
TGGGAAAATCTTTGCTAGCAGCAGGTATTACAGCTGTTGAAGGCGAGTTTCAAAGCCAAGAAGCCGTTTGCTTCTATGAT
TCTGCCGGCGTAGAAATTGCCCGAGGCTTGGTCAACTATAGTAGTGATGAGCTGCAGCGCATACAGGGGCGTCAATCGGA
GGATATTCCCCAGGTGCTTGGATATGCCGGAGCAGAAACTGTCGTGCATCGCGATAATCTGGTTATTACAGGATAA

Upstream 100 bases:

>100_bases
ATGACATGATTGAAATTGATATAGGTAAGGCATGATACCTGTATTCTTTTAATCTAGGTATCTGTGGGCCTAGTCTTCCC
CAGAAACGACCAGAGACTAT

Downstream 100 bases:

>100_bases
AGCCCCATATTTATGACCTTATACATCAAAATATCTTCAATATTCGCCCCACAGACCCGGTACGCCCTGTGAGTATTGAA
CTTCGAACTATATTTTTTGG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSQTLVLKIGTSSLTNPDTGHLALATIASLVEVLSQLRQEGNQVILVSSGAVGIGCARLGLAERPKAIALKQAVAAVGQG
RLMRIYDDFFTSLQQPIAQVLLTRGDLADRSRYINASNTLQELLKLGVIPIVNENDTVAVDELLRFGDNDTLSALVAGLV
QADWLFILTDVDRLYSADPRIQPDAQPIVTVERMEELAALNVNAGAQGSQWGTGGMATKISAAAIATNAGVRTVITQGKT
PENIPRILAGESLGTQFQPHPRPHNARKHWIAHALVPTGKLILDDGAAKAITTLGKSLLAAGITAVEGEFQSQEAVCFYD
SAGVEIARGLVNYSSDELQRIQGRQSEDIPQVLGYAGAETVVHRDNLVITG

Sequences:

>Translated_371_residues
MSQTLVLKIGTSSLTNPDTGHLALATIASLVEVLSQLRQEGNQVILVSSGAVGIGCARLGLAERPKAIALKQAVAAVGQG
RLMRIYDDFFTSLQQPIAQVLLTRGDLADRSRYINASNTLQELLKLGVIPIVNENDTVAVDELLRFGDNDTLSALVAGLV
QADWLFILTDVDRLYSADPRIQPDAQPIVTVERMEELAALNVNAGAQGSQWGTGGMATKISAAAIATNAGVRTVITQGKT
PENIPRILAGESLGTQFQPHPRPHNARKHWIAHALVPTGKLILDDGAAKAITTLGKSLLAAGITAVEGEFQSQEAVCFYD
SAGVEIARGLVNYSSDELQRIQGRQSEDIPQVLGYAGAETVVHRDNLVITG
>Mature_370_residues
SQTLVLKIGTSSLTNPDTGHLALATIASLVEVLSQLRQEGNQVILVSSGAVGIGCARLGLAERPKAIALKQAVAAVGQGR
LMRIYDDFFTSLQQPIAQVLLTRGDLADRSRYINASNTLQELLKLGVIPIVNENDTVAVDELLRFGDNDTLSALVAGLVQ
ADWLFILTDVDRLYSADPRIQPDAQPIVTVERMEELAALNVNAGAQGSQWGTGGMATKISAAAIATNAGVRTVITQGKTP
ENIPRILAGESLGTQFQPHPRPHNARKHWIAHALVPTGKLILDDGAAKAITTLGKSLLAAGITAVEGEFQSQEAVCFYDS
AGVEIARGLVNYSSDELQRIQGRQSEDIPQVLGYAGAETVVHRDNLVITG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=287, Percent_Identity=32.404181184669, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI21361368, Length=289, Percent_Identity=32.1799307958478, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=43.3242506811989, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17569969, Length=270, Percent_Identity=37.037037037037, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17569967, Length=270, Percent_Identity=37.037037037037, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=41.726618705036, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=38.5167464114833, Blast_Score=262, Evalue=6e-71,
Organism=Drosophila melanogaster, GI21357643, Length=295, Percent_Identity=33.8983050847458, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_ACAM1 (B0C1B5)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518968.1
- ProteinModelPortal:   B0C1B5
- GeneID:   5683478
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4676
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   AMAR329726:AM1_4676-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39423; Mature: 39292

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTLVLKIGTSSLTNPDTGHLALATIASLVEVLSQLRQEGNQVILVSSGAVGIGCARLG
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCC
LAERPKAIALKQAVAAVGQGRLMRIYDDFFTSLQQPIAQVLLTRGDLADRSRYINASNTL
CCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHH
QELLKLGVIPIVNENDTVAVDELLRFGDNDTLSALVAGLVQADWLFILTDVDRLYSADPR
HHHHHCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECHHHHHCCCCC
IQPDAQPIVTVERMEELAALNVNAGAQGSQWGTGGMATKISAAAIATNAGVRTVITQGKT
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCC
PENIPRILAGESLGTQFQPHPRPHNARKHWIAHALVPTGKLILDDGAAKAITTLGKSLLA
CCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
AGITAVEGEFQSQEAVCFYDSAGVEIARGLVNYSSDELQRIQGRQSEDIPQVLGYAGAET
HCHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCCC
VVHRDNLVITG
EEECCCEEEEC
>Mature Secondary Structure 
SQTLVLKIGTSSLTNPDTGHLALATIASLVEVLSQLRQEGNQVILVSSGAVGIGCARLG
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCC
LAERPKAIALKQAVAAVGQGRLMRIYDDFFTSLQQPIAQVLLTRGDLADRSRYINASNTL
CCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHH
QELLKLGVIPIVNENDTVAVDELLRFGDNDTLSALVAGLVQADWLFILTDVDRLYSADPR
HHHHHCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECHHHHHCCCCC
IQPDAQPIVTVERMEELAALNVNAGAQGSQWGTGGMATKISAAAIATNAGVRTVITQGKT
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCC
PENIPRILAGESLGTQFQPHPRPHNARKHWIAHALVPTGKLILDDGAAKAITTLGKSLLA
CCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
AGITAVEGEFQSQEAVCFYDSAGVEIARGLVNYSSDELQRIQGRQSEDIPQVLGYAGAET
HCHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCCC
VVHRDNLVITG
EEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA