Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 158337779

GI number: 158337779

Start: 4698045

End: 4698614

Strand: Reverse

Name: def [H]

Synonym: AM1_4663

Alternate gene names: 158337779

Gene position: 4698614-4698045 (Counterclockwise)

Preceding gene: 158337780

Following gene: 158337778

Centisome position: 72.24

GC content: 47.89

Gene sequence:

>570_bases
ATGTCTTCTGAAGTTCTGGTTGAAAAGCGCAAACTTAAAAATCCTCCTCTAAATATTCACACCCTTGGTGATCGCGTGCT
TAGACAATCAGCCAAGCGAGTGTCCAAGGTGGATGATGAAATCCGGGAATTGGCCCGTCAAATGCTGCAAACGATGTACA
GCGCTGACGGTATTGGTCTGGCAGCGCCCCAAGTGGCCGTAGGCAAGCAGCTAATCGTAGTGGATACTGACCCGGAAGAA
CCGGCTAATCAACCCATTATTCTGCTTAATCCTAAAATTCGTCGCCACAGCGATGACTTAGCGTTAGGTCAGGAAGGTTG
TCTGAGTATCCCTGGGGTATATTTAGATGTCCGACGGCCAGCCCAAATTGAAGTTGCTTATAAAGATGAGCAAGGTAGGC
CGCAAGTGATTGTGGCGACAGATCTGCTGGCGCGGGTCATTCAGCATGAAATGGATCATCTAACAGGCGTTATGTTTGTA
GACCGAGTAGAGAATAGCTTGTTGCTGAACCAAGAATTGACGAAGCAGGGTTTCTCAAAACAAGCAGTTAAGCCAGTTGA
AGCCGCCTAA

Upstream 100 bases:

>100_bases
TTGAGCTTTAGCAGTTTTGAGGGTCTAGCCTGGATTGAGTACAATTGAAGGATAGAGAAATTCTCTACGTCTTTTATCGT
TCCACCCTATACGCTTGGTT

Downstream 100 bases:

>100_bases
GATTTTAGAGCCGAGGTTTTTTGCTTTGGTCTCAGCCTTCGCGATCTGGTGCACCATCAGAGTCGTTATTTTAGGTGAAC
AAACGCGATATTTTAGGTGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSSEVLVEKRKLKNPPLNIHTLGDRVLRQSAKRVSKVDDEIRELARQMLQTMYSADGIGLAAPQVAVGKQLIVVDTDPEE
PANQPIILLNPKIRRHSDDLALGQEGCLSIPGVYLDVRRPAQIEVAYKDEQGRPQVIVATDLLARVIQHEMDHLTGVMFV
DRVENSLLLNQELTKQGFSKQAVKPVEAA

Sequences:

>Translated_189_residues
MSSEVLVEKRKLKNPPLNIHTLGDRVLRQSAKRVSKVDDEIRELARQMLQTMYSADGIGLAAPQVAVGKQLIVVDTDPEE
PANQPIILLNPKIRRHSDDLALGQEGCLSIPGVYLDVRRPAQIEVAYKDEQGRPQVIVATDLLARVIQHEMDHLTGVMFV
DRVENSLLLNQELTKQGFSKQAVKPVEAA
>Mature_188_residues
SSEVLVEKRKLKNPPLNIHTLGDRVLRQSAKRVSKVDDEIRELARQMLQTMYSADGIGLAAPQVAVGKQLIVVDTDPEEP
ANQPIILLNPKIRRHSDDLALGQEGCLSIPGVYLDVRRPAQIEVAYKDEQGRPQVIVATDLLARVIQHEMDHLTGVMFVD
RVENSLLLNQELTKQGFSKQAVKPVEAA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=179, Percent_Identity=30.1675977653631, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1789682, Length=145, Percent_Identity=39.3103448275862, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645726, Length=172, Percent_Identity=29.6511627906977, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24645728, Length=190, Percent_Identity=29.4736842105263, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21016; Mature: 20884

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEVLVEKRKLKNPPLNIHTLGDRVLRQSAKRVSKVDDEIRELARQMLQTMYSADGIGL
CCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AAPQVAVGKQLIVVDTDPEEPANQPIILLNPKIRRHSDDLALGQEGCLSIPGVYLDVRRP
CCCHHHHCCEEEEEECCCCCCCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCEEEEECCC
AQIEVAYKDEQGRPQVIVATDLLARVIQHEMDHLTGVMFVDRVENSLLLNQELTKQGFSK
CEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QAVKPVEAA
HHCCCCCCC
>Mature Secondary Structure 
SSEVLVEKRKLKNPPLNIHTLGDRVLRQSAKRVSKVDDEIRELARQMLQTMYSADGIGL
CCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AAPQVAVGKQLIVVDTDPEEPANQPIILLNPKIRRHSDDLALGQEGCLSIPGVYLDVRRP
CCCHHHHCCEEEEEECCCCCCCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCEEEEECCC
AQIEVAYKDEQGRPQVIVATDLLARVIQHEMDHLTGVMFVDRVENSLLLNQELTKQGFSK
CEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
QAVKPVEAA
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]