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The map label for this gene is rpoD [H]

Identifier: 158337702

GI number: 158337702

Start: 4620308

End: 4621264

Strand: Direct

Name: rpoD [H]

Synonym: AM1_4586

Alternate gene names: 158337702

Gene position: 4620308-4621264 (Clockwise)

Preceding gene: 158337701

Following gene: 158337704

Centisome position: 71.04

GC content: 47.34

Gene sequence:

>957_bases
ATGAAAAATTCATCTGTGAGCAAAGATTCTATTACGACCTATCTGCGAGAGATCGGCCGCTTCCCTCTGTTGAATCATGA
AGAGGAAATTATCTTTGGTAAGCAGGTGCAACGGTTTGTAGCTTTGCAAGATCTCAAGCAAGATCTGCAAGAGCACCTAG
ATCGGGAACCGACCCAGGCCGAATGGGCAGATAAAGCTGAGATATCGGTCTCACAACTACAGAAAGAGCTGAAGTTAGGC
CATAAAGCCAATCGCAAAATGGTGGAATCCAATTTGCGTCTAGTGGTCTCGATTGCCAAGAAGTACAACAAAGCGAAAGT
TGAATTTTTGGATTTGATTCAAGAAGGCACGATTGGCTTGCAACGGGGTGTCGAAAAGTTTGACCCGATGAAAGGCTATC
GCTTTTCTACCTATGCCTACTGGTGGATTCGTCAAGCCATGACCCGTGCGATCGCAGAACAAGAACGAACCATTCGCTTA
CCGATTCACATCAATGAAAAACTTAGTAAGCTGCGCAAGGTCAGACGCCAGCTGTCGCAAAAGCTCCATCGCGACCCCAC
TGTGCAAGAGCTAGCGGTAGAGATGGACCTTGCTCCTAAAAAGATTGAAGACTATCTCAACTACGCTCGTCGCTCGATCT
CGTTAGATATTCAGATTGGTGATCAGCAAAATACAGAGCTATGGGAGTTACTCGAAGATCCCAATGAATCGCCCGACACC
TATGTCACGGCCTCTTCTCTGCGATCTGATTTAGCCAAGCTGCTTCAAGATCTCAGCCCGCAACAGCGAGAAGTGATTAC
TTTACGATACGGCCTGATTGATCAAAATCCCTTGACCTTAGCGAAGGTGGGTGAGCGTCTGAACGTTAGCCGGGAGTGGG
TCCGCCGGGTAGAGCGGGAAGCCTTTAAGATTTTGCGATCGCAAAAAGTGATGCTGCAAGAATATATGGCGATTTAG

Upstream 100 bases:

>100_bases
GAAACATATAGCTACCCTCAATATTCTCTTCTTCCTTGTCTTTTAGCCCCAGCTTCTCGCTGGGGTCTTTTATATCCACT
AATTCCAAGTTTCCGTAGAC

Downstream 100 bases:

>100_bases
CTACGAGAATGCGGCCCGCCTCCCACCATTAGTTACTTTCAATTGTGTAGGGGTTGATTCCCTGCTGCTTGCAGCGAAGA
AATGGGATACGGTTTGGTAG

Product: RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKNSSVSKDSITTYLREIGRFPLLNHEEEIIFGKQVQRFVALQDLKQDLQEHLDREPTQAEWADKAEISVSQLQKELKLG
HKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGLQRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRL
PIHINEKLSKLRKVRRQLSQKLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
YVTASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVEREAFKILRSQKVMLQEYMAI

Sequences:

>Translated_318_residues
MKNSSVSKDSITTYLREIGRFPLLNHEEEIIFGKQVQRFVALQDLKQDLQEHLDREPTQAEWADKAEISVSQLQKELKLG
HKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGLQRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRL
PIHINEKLSKLRKVRRQLSQKLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
YVTASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVEREAFKILRSQKVMLQEYMAI
>Mature_318_residues
MKNSSVSKDSITTYLREIGRFPLLNHEEEIIFGKQVQRFVALQDLKQDLQEHLDREPTQAEWADKAEISVSQLQKELKLG
HKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGLQRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRL
PIHINEKLSKLRKVRRQLSQKLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
YVTASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVEREAFKILRSQKVMLQEYMAI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=42.5619834710744, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1789098, Length=299, Percent_Identity=36.7892976588629, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=25.5144032921811, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788231, Length=214, Percent_Identity=28.9719626168224, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37289; Mature: 37289

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSSVSKDSITTYLREIGRFPLLNHEEEIIFGKQVQRFVALQDLKQDLQEHLDREPTQA
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EWADKAEISVSQLQKELKLGHKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGL
HHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
QRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRLPIHINEKLSKLRKVRRQLSQ
HHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHH
KLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
HHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCC
YVTASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVERE
EEEHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
AFKILRSQKVMLQEYMAI
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNSSVSKDSITTYLREIGRFPLLNHEEEIIFGKQVQRFVALQDLKQDLQEHLDREPTQA
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EWADKAEISVSQLQKELKLGHKANRKMVESNLRLVVSIAKKYNKAKVEFLDLIQEGTIGL
HHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
QRGVEKFDPMKGYRFSTYAYWWIRQAMTRAIAEQERTIRLPIHINEKLSKLRKVRRQLSQ
HHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHH
KLHRDPTVQELAVEMDLAPKKIEDYLNYARRSISLDIQIGDQQNTELWELLEDPNESPDT
HHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCC
YVTASSLRSDLAKLLQDLSPQQREVITLRYGLIDQNPLTLAKVGERLNVSREWVRRVERE
EEEHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
AFKILRSQKVMLQEYMAI
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA