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The map label for this gene is purU [H]

Identifier: 158337630

GI number: 158337630

Start: 4535945

End: 4536799

Strand: Reverse

Name: purU [H]

Synonym: AM1_4512

Alternate gene names: 158337630

Gene position: 4536799-4535945 (Counterclockwise)

Preceding gene: 158337633

Following gene: 158337629

Centisome position: 69.76

GC content: 48.07

Gene sequence:

>855_bases
ATGTCTGCACCAACAGCAACGCTCCTCATTTCTTGCCCAGATCAACAAGGGCTAGTGGCTAAAATTGCCCACTTTATTGC
AACTCATCATGGCAACATCATCCATGCCGATCACCATACGGATTTCACCAATCAATTATTTCTCAGTCGTCTGGAATGGA
GTTTAGAGGGGTTTCAGTTATCGCGATCGCAAATCGGTACTCAATTCTCTGAACTGGCAGAATCCTTACGGGCCAACTGG
CAACTGCACTTTTCCGACTGCAAACCTCGCATGTCTATTTGGGTAAGCAAGCAGGATCACTGCCTGCTGGATTTACTGTG
GCGACAACAGGCTGGCGATCTTCCCGTCGAAATTCCCCTGATCATCAGCAATCATGACACCCTGCAACCGATTGCCGAAC
AATTCAACATCGACTTTTACCACTTACCCATCAACAAAGAGAGTAAAGCGCGGCAGGAGAAACAACAGCTTGCCTTACTC
AAGCAATACAACATTGACTTAGTGGTCCTTGCCAAGTACATGCAAATCCTCAGCCCTCAATTCATCGCCGCCTTCTCTTC
CACCATCAATATTCACCACTCCTTCCTGCCTGCCTTTCCGGGGGCAAATCCCTACCAAAGAGCCTATAAGCGTGGCGTCA
AAATTATTGGTGCGACAGCCCACTATGTGACCGAAGAGCTGGATGAAGGCCCCATTATTGAACAAGAAGTCGTACGAGTC
AGTCACCGGGATTCCAGTGATGAATTTATCCGTAAGGGCAAAGATGTAGAACGTTCCGCATTGGCCAGAGCAGTTCGTCT
TCACCTGCAAAACCGGGTCTTGGTCTATAACCATCGCACCGTTGTTTTTGATTGA

Upstream 100 bases:

>100_bases
CACGCTTTGTCTTTGCAAGCTCAATCGGCTCAATGCATGGACTAGTCACTGGGAGACAAAATGTGTAAGCTTGGCAGCAG
TTAAAACAATGGGATCGGCT

Downstream 100 bases:

>100_bases
GTCAGTTTCCTTAGGCTAGGATAGAGTCCGGTCTCACCTGAATAGCCATTTCTCTGGGTCTTATCGCGGCTGCTACTGCT
GATAAATCAGAGAAGATTAA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSAPTATLLISCPDQQGLVAKIAHFIATHHGNIIHADHHTDFTNQLFLSRLEWSLEGFQLSRSQIGTQFSELAESLRANW
QLHFSDCKPRMSIWVSKQDHCLLDLLWRQQAGDLPVEIPLIISNHDTLQPIAEQFNIDFYHLPINKESKARQEKQQLALL
KQYNIDLVVLAKYMQILSPQFIAAFSSTINIHHSFLPAFPGANPYQRAYKRGVKIIGATAHYVTEELDEGPIIEQEVVRV
SHRDSSDEFIRKGKDVERSALARAVRLHLQNRVLVYNHRTVVFD

Sequences:

>Translated_284_residues
MSAPTATLLISCPDQQGLVAKIAHFIATHHGNIIHADHHTDFTNQLFLSRLEWSLEGFQLSRSQIGTQFSELAESLRANW
QLHFSDCKPRMSIWVSKQDHCLLDLLWRQQAGDLPVEIPLIISNHDTLQPIAEQFNIDFYHLPINKESKARQEKQQLALL
KQYNIDLVVLAKYMQILSPQFIAAFSSTINIHHSFLPAFPGANPYQRAYKRGVKIIGATAHYVTEELDEGPIIEQEVVRV
SHRDSSDEFIRKGKDVERSALARAVRLHLQNRVLVYNHRTVVFD
>Mature_283_residues
SAPTATLLISCPDQQGLVAKIAHFIATHHGNIIHADHHTDFTNQLFLSRLEWSLEGFQLSRSQIGTQFSELAESLRANWQ
LHFSDCKPRMSIWVSKQDHCLLDLLWRQQAGDLPVEIPLIISNHDTLQPIAEQFNIDFYHLPINKESKARQEKQQLALLK
QYNIDLVVLAKYMQILSPQFIAAFSSTINIHHSFLPAFPGANPYQRAYKRGVKIIGATAHYVTEELDEGPIIEQEVVRVS
HRDSSDEFIRKGKDVERSALARAVRLHLQNRVLVYNHRTVVFD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=194, Percent_Identity=29.8969072164948, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI209869995, Length=194, Percent_Identity=29.8969072164948, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI209869993, Length=194, Percent_Identity=29.8969072164948, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1787483, Length=274, Percent_Identity=43.4306569343066, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1788846, Length=107, Percent_Identity=36.4485981308411, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=159, Percent_Identity=36.4779874213836, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24582400, Length=208, Percent_Identity=27.8846153846154, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32518; Mature: 32386

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTATLLISCPDQQGLVAKIAHFIATHHGNIIHADHHTDFTNQLFLSRLEWSLEGFQL
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
SRSQIGTQFSELAESLRANWQLHFSDCKPRMSIWVSKQDHCLLDLLWRQQAGDLPVEIPL
HHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
IISNHDTLQPIAEQFNIDFYHLPINKESKARQEKQQLALLKQYNIDLVVLAKYMQILSPQ
EECCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHH
FIAAFSSTINIHHSFLPAFPGANPYQRAYKRGVKIIGATAHYVTEELDEGPIIEQEVVRV
HHHHHHHHHEEHHHHCCCCCCCCHHHHHHHCCCEEEECHHHHHHHHCCCCCCHHHHHHHH
SHRDSSDEFIRKGKDVERSALARAVRLHLQNRVLVYNHRTVVFD
HCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECEEEEEC
>Mature Secondary Structure 
SAPTATLLISCPDQQGLVAKIAHFIATHHGNIIHADHHTDFTNQLFLSRLEWSLEGFQL
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
SRSQIGTQFSELAESLRANWQLHFSDCKPRMSIWVSKQDHCLLDLLWRQQAGDLPVEIPL
HHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEE
IISNHDTLQPIAEQFNIDFYHLPINKESKARQEKQQLALLKQYNIDLVVLAKYMQILSPQ
EECCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHH
FIAAFSSTINIHHSFLPAFPGANPYQRAYKRGVKIIGATAHYVTEELDEGPIIEQEVVRV
HHHHHHHHHEEHHHHCCCCCCCCHHHHHHHCCCEEEECHHHHHHHHCCCCCCHHHHHHHH
SHRDSSDEFIRKGKDVERSALARAVRLHLQNRVLVYNHRTVVFD
HCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]