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The map label for this gene is gyrB [H]

Identifier: 158337618

GI number: 158337618

Start: 4526040

End: 4527980

Strand: Direct

Name: gyrB [H]

Synonym: AM1_4499

Alternate gene names: 158337618

Gene position: 4526040-4527980 (Clockwise)

Preceding gene: 158337615

Following gene: 158337619

Centisome position: 69.59

GC content: 50.85

Gene sequence:

>1941_bases
ATGGCCACAAACTACAGTGCAGATCAGATTCAAGTCCTGGAGGGATTGGAACCGGTTCGGAAACGTCCTGGGATGTATAT
CGGCAGCACTGGCCCCAGAGGTTTGCACCACTTAGTGTACGAGGTGGTCGATAACTCTGTCGATGAAGCCCTTGCGGGTC
ACTGTAAGCATATTCACATTTATTTAAATGAGGACGGTTCTGTTCGAGTCACCGATGATGGCCGCGGCATTCCCACCGAC
AAGCATCCTACTACAGGCAAATCGGCCCTAGAAACCGTGATGACGGTTCTCCATGCTGGCGGAAAGTTTGATGGTGATAG
CTATAAAGTGTCGGGCGGACTCCATGGGGTAGGTATTTCCGTTGTCAACGCCCTCTCCGAATGGGTGGAAGCGAAAGTCT
GGCGTAATGGCAAGGTTCATACCCAACGATTTGAGCGAGGCATTCCGAAATCTGAGTTAGTCGTCGAACCCACCGATCGC
AAAGAGTCGGGAACGGCCATTACGTTTTTACCCGACGACACGATCTTTACCGTTACCACAGAATTTGACTATAAGACCCT
AGCCAGCCGCTTGCGAGAACTCGCCTACCTGAATGCCGGGGTAGAAATTTCTTTCCACGATAACCGTCTGGAACTCCTAG
AAGCCGACGAGCCCAAGGTTGAGACCTACCGCTACGATGGCGGCATCAAAGAATATGTGGACTATATGAACCGGGACAAA
GAGCCCATTCATGAAGAATGCATCTATGTAGAAGGGGAACGCAATAATGTTCAGGTAGAGGTGGCCCTACAATGGTGCCG
AGATGCCTATTCTGACAACCTCTTGGGGTTTGCCAACAATATTCGTACCATTGATGGCGGTACCCACTTAGAAGGTCTAA
AGACGGTGCTTACCCGGACCATGAACTCCCTGGCTCGAAAGCGCAGCAAGCTTAAAGATGGTGATTCTAACCTTGGGGGT
GAACATATCCGGGAAGGCTTAACCGCCGTTATCTCCGTCAAAGTGCCGGAACCCGAATTTGAAGGCCAGACTAAAACCAA
ATTGGGTAATACAGAAGTGCGGGGGATTGTTGATTCCCTCGTGGGTGAAGTACTAACGGAGTATTTAGATTTTCACCCCA
ATGTCGCTGATTCTGTAATTGAAAAAGCCCTGCAAGCCTTTAACGCCGCTGAAGCCGCTCGTCGGGCACGAGAGCTAGTC
CGCCGTAAATCGGTACTGGAATCCTCAACTCTACCTGGGAAGCTCGCAGACTGTAGCTCCCGCGACCCTAGTACTTCAGA
GATTTACATTGTGGAGGGAGATTCTGCGGGAGGGAGTGCGAAGCAAGGACGCGATCGCGGATTCCAAGCCATTCTGCCTT
TGCGAGGTAAAATCCTCAACATCGAGAAGACCGATGATGCCAAGATCTACAAAAACAATGAGATTCAGTCTCTGATTACT
GCCTTGGGGCTCGGCATTAAAGGCGAGGAATTCGACGTTTCCCAGCTTCGCTATCATCGGATCATTCTGATGACGGACGC
TGATGTGGATGGCGCCCATATTCGGACGCTCCTACTCACCTTCTTCTACCGCTATCAGCGAGAACTGATTGACCAGGGCT
ATATCTATATTGCCTGTCCACCCCTCTACAAAATTGAGCGAGGTCGATCTCACTACTATTGCTATAGCGATCGCGAACTT
CAGAACCTGGTTCAACATGAGTTCCCTGCGAATGCCAACTACACGATTCAGCGGTTTAAGGGCTTAGGGGAAATGATGCC
AACCCAGCTCTGGGATACCACCATGAATCCCGAAACCCGAACCCTCAAGCAAATTGATATTGAGGATGCGGCGGAGGCGG
ATCGGATTTTCACGATCCTGATGGGCGATCGCGTCGCTCCTCGCCGGGAATTTATCGAGACCCACGGTCCCCGACTCAAC
CTAGCTGAACTAGATATCTAG

Upstream 100 bases:

>100_bases
CTAAAATCGCTTAAAAGGCATTTGTGATAGAATTTGGGGGGTTTTTTGTATTTGCCCAAATCAACACTGCTTTAAGTCGC
TTATAGGAGCGCTTCACTAC

Downstream 100 bases:

>100_bases
GAGCCGCCATGACTCAATCCATCAAAGATCCACTCCGTTGGACGACAGCAGATATTGAGTCCTTAGCAGCGGATGAATGG
AAACGTTATGAAATTATTGA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MATNYSADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCKHIHIYLNEDGSVRVTDDGRGIPTD
KHPTTGKSALETVMTVLHAGGKFDGDSYKVSGGLHGVGISVVNALSEWVEAKVWRNGKVHTQRFERGIPKSELVVEPTDR
KESGTAITFLPDDTIFTVTTEFDYKTLASRLRELAYLNAGVEISFHDNRLELLEADEPKVETYRYDGGIKEYVDYMNRDK
EPIHEECIYVEGERNNVQVEVALQWCRDAYSDNLLGFANNIRTIDGGTHLEGLKTVLTRTMNSLARKRSKLKDGDSNLGG
EHIREGLTAVISVKVPEPEFEGQTKTKLGNTEVRGIVDSLVGEVLTEYLDFHPNVADSVIEKALQAFNAAEAARRARELV
RRKSVLESSTLPGKLADCSSRDPSTSEIYIVEGDSAGGSAKQGRDRGFQAILPLRGKILNIEKTDDAKIYKNNEIQSLIT
ALGLGIKGEEFDVSQLRYHRIILMTDADVDGAHIRTLLLTFFYRYQRELIDQGYIYIACPPLYKIERGRSHYYCYSDREL
QNLVQHEFPANANYTIQRFKGLGEMMPTQLWDTTMNPETRTLKQIDIEDAAEADRIFTILMGDRVAPRREFIETHGPRLN
LAELDI

Sequences:

>Translated_646_residues
MATNYSADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCKHIHIYLNEDGSVRVTDDGRGIPTD
KHPTTGKSALETVMTVLHAGGKFDGDSYKVSGGLHGVGISVVNALSEWVEAKVWRNGKVHTQRFERGIPKSELVVEPTDR
KESGTAITFLPDDTIFTVTTEFDYKTLASRLRELAYLNAGVEISFHDNRLELLEADEPKVETYRYDGGIKEYVDYMNRDK
EPIHEECIYVEGERNNVQVEVALQWCRDAYSDNLLGFANNIRTIDGGTHLEGLKTVLTRTMNSLARKRSKLKDGDSNLGG
EHIREGLTAVISVKVPEPEFEGQTKTKLGNTEVRGIVDSLVGEVLTEYLDFHPNVADSVIEKALQAFNAAEAARRARELV
RRKSVLESSTLPGKLADCSSRDPSTSEIYIVEGDSAGGSAKQGRDRGFQAILPLRGKILNIEKTDDAKIYKNNEIQSLIT
ALGLGIKGEEFDVSQLRYHRIILMTDADVDGAHIRTLLLTFFYRYQRELIDQGYIYIACPPLYKIERGRSHYYCYSDREL
QNLVQHEFPANANYTIQRFKGLGEMMPTQLWDTTMNPETRTLKQIDIEDAAEADRIFTILMGDRVAPRREFIETHGPRLN
LAELDI
>Mature_645_residues
ATNYSADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCKHIHIYLNEDGSVRVTDDGRGIPTDK
HPTTGKSALETVMTVLHAGGKFDGDSYKVSGGLHGVGISVVNALSEWVEAKVWRNGKVHTQRFERGIPKSELVVEPTDRK
ESGTAITFLPDDTIFTVTTEFDYKTLASRLRELAYLNAGVEISFHDNRLELLEADEPKVETYRYDGGIKEYVDYMNRDKE
PIHEECIYVEGERNNVQVEVALQWCRDAYSDNLLGFANNIRTIDGGTHLEGLKTVLTRTMNSLARKRSKLKDGDSNLGGE
HIREGLTAVISVKVPEPEFEGQTKTKLGNTEVRGIVDSLVGEVLTEYLDFHPNVADSVIEKALQAFNAAEAARRARELVR
RKSVLESSTLPGKLADCSSRDPSTSEIYIVEGDSAGGSAKQGRDRGFQAILPLRGKILNIEKTDDAKIYKNNEIQSLITA
LGLGIKGEEFDVSQLRYHRIILMTDADVDGAHIRTLLLTFFYRYQRELIDQGYIYIACPPLYKIERGRSHYYCYSDRELQ
NLVQHEFPANANYTIQRFKGLGEMMPTQLWDTTMNPETRTLKQIDIEDAAEADRIFTILMGDRVAPRREFIETHGPRLNL
AELDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=626, Percent_Identity=23.482428115016, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI19913408, Length=624, Percent_Identity=24.3589743589744, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI48994957, Length=561, Percent_Identity=53.475935828877, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789408, Length=652, Percent_Identity=39.4171779141104, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17535065, Length=590, Percent_Identity=23.728813559322, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=549, Percent_Identity=24.0437158469945, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=181, Percent_Identity=28.1767955801105, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=684, Percent_Identity=24.8538011695906, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17136538, Length=567, Percent_Identity=24.8677248677249, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72542; Mature: 72410

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNYSADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCKHIHI
CCCCCCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHCCCEEEEE
YLNEDGSVRVTDDGRGIPTDKHPTTGKSALETVMTVLHAGGKFDGDSYKVSGGLHGVGIS
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEWVEAKVWRNGKVHTQRFERGIPKSELVVEPTDRKESGTAITFLPDDTIFTVTT
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCEEEEECCCEEEEEEE
EFDYKTLASRLRELAYLNAGVEISFHDNRLELLEADEPKVETYRYDGGIKEYVDYMNRDK
CCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCEEEEECCCHHHHHHHHCCCC
EPIHEECIYVEGERNNVQVEVALQWCRDAYSDNLLGFANNIRTIDGGTHLEGLKTVLTRT
CCCCCCCEEEECCCCCEEEEEHHHHHHHHHCCCCEECCCCCEECCCCCHHHHHHHHHHHH
MNSLARKRSKLKDGDSNLGGEHIREGLTAVISVKVPEPEFEGQTKTKLGNTEVRGIVDSL
HHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHH
VGEVLTEYLDFHPNVADSVIEKALQAFNAAEAARRARELVRRKSVLESSTLPGKLADCSS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RDPSTSEIYIVEGDSAGGSAKQGRDRGFQAILPLRGKILNIEKTDDAKIYKNNEIQSLIT
CCCCCCEEEEEECCCCCCCCCCCHHCCCEEEECCCCEEEEEECCCCCEEEECCHHHHHHH
ALGLGIKGEEFDVSQLRYHRIILMTDADVDGAHIRTLLLTFFYRYQRELIDQGYIYIACP
HHCCCCCCCCCCHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLYKIERGRSHYYCYSDRELQNLVQHEFPANANYTIQRFKGLGEMMPTQLWDTTMNPETR
CCHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCHH
TLKQIDIEDAAEADRIFTILMGDRVAPRREFIETHGPRLNLAELDI
HHHHCCCHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ATNYSADQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCKHIHI
CCCCCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHCCCEEEEE
YLNEDGSVRVTDDGRGIPTDKHPTTGKSALETVMTVLHAGGKFDGDSYKVSGGLHGVGIS
EECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEWVEAKVWRNGKVHTQRFERGIPKSELVVEPTDRKESGTAITFLPDDTIFTVTT
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCEEEEECCCEEEEEEE
EFDYKTLASRLRELAYLNAGVEISFHDNRLELLEADEPKVETYRYDGGIKEYVDYMNRDK
CCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCEEEEECCCHHHHHHHHCCCC
EPIHEECIYVEGERNNVQVEVALQWCRDAYSDNLLGFANNIRTIDGGTHLEGLKTVLTRT
CCCCCCCEEEECCCCCEEEEEHHHHHHHHHCCCCEECCCCCEECCCCCHHHHHHHHHHHH
MNSLARKRSKLKDGDSNLGGEHIREGLTAVISVKVPEPEFEGQTKTKLGNTEVRGIVDSL
HHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHH
VGEVLTEYLDFHPNVADSVIEKALQAFNAAEAARRARELVRRKSVLESSTLPGKLADCSS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RDPSTSEIYIVEGDSAGGSAKQGRDRGFQAILPLRGKILNIEKTDDAKIYKNNEIQSLIT
CCCCCCEEEEEECCCCCCCCCCCHHCCCEEEECCCCEEEEEECCCCCEEEECCHHHHHHH
ALGLGIKGEEFDVSQLRYHRIILMTDADVDGAHIRTLLLTFFYRYQRELIDQGYIYIACP
HHCCCCCCCCCCHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLYKIERGRSHYYCYSDRELQNLVQHEFPANANYTIQRFKGLGEMMPTQLWDTTMNPETR
CCHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCHH
TLKQIDIEDAAEADRIFTILMGDRVAPRREFIETHGPRLNLAELDI
HHHHCCCHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]