Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 158337510

GI number: 158337510

Start: 4419271

End: 4419996

Strand: Direct

Name: tpiA

Synonym: AM1_4389

Alternate gene names: 158337510

Gene position: 4419271-4419996 (Clockwise)

Preceding gene: 158337509

Following gene: 158337511

Centisome position: 67.95

GC content: 50.41

Gene sequence:

>726_bases
GTGCGAAAAATTGCGATCGCAGGCAACTGGAAAATGCACAAAACTCAGGCAGAGGCCCTGGAGTTTTTGCAAACCTTTCT
ACCCCTGCTACAAGATACCCCTGAAGATCGAGATGTGATTCTCTGTGCACCTTTCACCACATTGACGGCCCTCTCCAAGA
ACTTACATGGCAGTCGGGTGCAAGTCGGGGCGCAAAATATCCACTGGGAAGATACCGGCGCGTTTACGGGCGAAATCTCG
GGGCCGATGCTGCTGGAAACTGGCGTCCGCTATGTCGTTGTTGGTCACAGTGAACGTCGCCAGTTTTTTGGTGAAACGGA
TGCTACCGTGAATCAGCGACTCAAGGCTGCTCAAAACCATCGCCTGACTCCAATTCTCTGTGTCGGTGAATCAAAGGCTC
AGCGAGATGCCAATGAGACCGAAACGGTTATCTTTGAGCAGCTTGAAAAAGGTCTGGTGGGTGTCGATCAAAAGAACTTA
ATCATTGCCTACGAACCTATTTGGGCCATCGGCACAGGGGATACCTGTGCGAGTAGTGAAGCCAATCGAGTCATTGGTTT
AATCCGTTCTCGACTAACCAACCACGATGTCACCATTCAATATGGTGGGTCGGTTAAGCCAGATAATGTGGATGAAATTA
TGGCCCAGCCAGAAATAGATGGTGCTCTGGTCGGTGGCGCTAGTTTGGCTGGCGACGGTTTCGCGCGGGTTGTTAATTAT
CAGTAA

Upstream 100 bases:

>100_bases
CAACGGACCAGTGTTCGATCCCTCGGTCCTAGTAATGCGTCACCCAAGCCCTTACACTTAATAGTGCATTAACTTTAATC
GAAACTTAGATAAAACTACC

Downstream 100 bases:

>100_bases
GGATCAGGTGTTGGTGAGCATTGTATTGCGTTCCCAATATCTGTAGCAATTGGCTATCTAGGCATCTAAGATACCGGCCA
TTTGAGTTTGAGTCGCTAGA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MRKIAIAGNWKMHKTQAEALEFLQTFLPLLQDTPEDRDVILCAPFTTLTALSKNLHGSRVQVGAQNIHWEDTGAFTGEIS
GPMLLETGVRYVVVGHSERRQFFGETDATVNQRLKAAQNHRLTPILCVGESKAQRDANETETVIFEQLEKGLVGVDQKNL
IIAYEPIWAIGTGDTCASSEANRVIGLIRSRLTNHDVTIQYGGSVKPDNVDEIMAQPEIDGALVGGASLAGDGFARVVNY
Q

Sequences:

>Translated_241_residues
MRKIAIAGNWKMHKTQAEALEFLQTFLPLLQDTPEDRDVILCAPFTTLTALSKNLHGSRVQVGAQNIHWEDTGAFTGEIS
GPMLLETGVRYVVVGHSERRQFFGETDATVNQRLKAAQNHRLTPILCVGESKAQRDANETETVIFEQLEKGLVGVDQKNL
IIAYEPIWAIGTGDTCASSEANRVIGLIRSRLTNHDVTIQYGGSVKPDNVDEIMAQPEIDGALVGGASLAGDGFARVVNY
Q
>Mature_241_residues
MRKIAIAGNWKMHKTQAEALEFLQTFLPLLQDTPEDRDVILCAPFTTLTALSKNLHGSRVQVGAQNIHWEDTGAFTGEIS
GPMLLETGVRYVVVGHSERRQFFGETDATVNQRLKAAQNHRLTPILCVGESKAQRDANETETVIFEQLEKGLVGVDQKNL
IIAYEPIWAIGTGDTCASSEANRVIGLIRSRLTNHDVTIQYGGSVKPDNVDEIMAQPEIDGALVGGASLAGDGFARVVNY
Q

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:
GO:0003824: Triosephosphate isomerase
GO:0004807: Triosephosphate isomerase
GO:0005737: Triosephosphate isomerase
GO:0006094: Triosephosphate isomerase
GO:0006096: Triosephosphate isomerase
GO:0006098: Triosephosphate isomerase
GO:0008152: Triosephosphate isomerase
GO:0016853: Triosephosphate isomerase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=38.2113821138211, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=38.2113821138211, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1790353, Length=248, Percent_Identity=37.5, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17536593, Length=246, Percent_Identity=41.0569105691057, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320255, Length=249, Percent_Identity=34.5381526104418, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=38.2113821138211, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=38.2113821138211, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI28572004, Length=246, Percent_Identity=38.2113821138211, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_ACAM1 (B0CEX1)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518685.1
- ProteinModelPortal:   B0CEX1
- SMR:   B0CEX1
- GeneID:   5683192
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4389
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- BioCyc:   AMAR329726:AM1_4389-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26345; Mature: 26345

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 165-165 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIAIAGNWKMHKTQAEALEFLQTFLPLLQDTPEDRDVILCAPFTTLTALSKNLHGSRV
CCEEEEECCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCCCEE
QVGAQNIHWEDTGAFTGEISGPMLLETGVRYVVVGHSERRQFFGETDATVNQRLKAAQNH
EECCCCCCCCCCCCEECCCCCCEEEECCCEEEEECCHHHHHHCCCCCHHHHHHHHHHHCC
RLTPILCVGESKAQRDANETETVIFEQLEKGLVGVDQKNLIIAYEPIWAIGTGDTCASSE
CCCEEEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECCCCCCCCH
ANRVIGLIRSRLTNHDVTIQYGGSVKPDNVDEIMAQPEIDGALVGGASLAGDGFARVVNY
HHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCCCCEEECCCCCCCCCCEEEECC
Q
C
>Mature Secondary Structure
MRKIAIAGNWKMHKTQAEALEFLQTFLPLLQDTPEDRDVILCAPFTTLTALSKNLHGSRV
CCEEEEECCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCCCEE
QVGAQNIHWEDTGAFTGEISGPMLLETGVRYVVVGHSERRQFFGETDATVNQRLKAAQNH
EECCCCCCCCCCCCEECCCCCCEEEECCCEEEEECCHHHHHHCCCCCHHHHHHHHHHHCC
RLTPILCVGESKAQRDANETETVIFEQLEKGLVGVDQKNLIIAYEPIWAIGTGDTCASSE
CCCEEEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECCCCCCCCH
ANRVIGLIRSRLTNHDVTIQYGGSVKPDNVDEIMAQPEIDGALVGGASLAGDGFARVVNY
HHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCCCCEEECCCCCCCCCCEEEECC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA