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The map label for this gene is folE

Identifier: 158337473

GI number: 158337473

Start: 4381340

End: 4381774

Strand: Reverse

Name: folE

Synonym: AM1_4352

Alternate gene names: 158337473

Gene position: 4381774-4381340 (Counterclockwise)

Preceding gene: 158337476

Following gene: 158337463

Centisome position: 67.37

GC content: 46.9

Gene sequence:

>435_bases
ATGAGCCAATCTCCCATCCAAAATCCTACTTCTGACCCCAACGCCCAGTCTGTACAAGAGACTTCAGAATCCAAATATGG
GGAACGCCAAATTGCAGAAGGAACACTGATTACTTTCCCGAACCCTCGCGTGGGACGTCGTTATGATATTCACATTACGC
TGCCAGAATTTACCTGCAAATGCCCGTTTTCTGGATATCCTGATTTCGCCACCATTCATCTCACCTATGTCCCAGATCAG
CGCGTTGTGGAGCTGAAAGCGTTAAAACTCTACATCAACAGCTATCGCGACCGCTATATTTCCCATGAGGAATCTGTGAA
CCAAATTTTGGATGATATTGTGGCCGCCTGTGATCCCTTGGAGATCACGGTAAAAGGTGACTTCCTGCCCAGAGGAAACG
TTCACACAGTGATTGAAGTCCACCACCAAAAATAG

Upstream 100 bases:

>100_bases
CTGCTTTGCTGCCACCGTCTACGCTATAACAAGTTTAGTCGTTTCTAACTGTAACCTTCTTAAATAGCAGTGGGGTGACC
CACACTTGCTACCAAACCTC

Downstream 100 bases:

>100_bases
AGATTAGGGTTTAGGCGTGATCGCTTTCGCCATGTAAATATCCGGCTTACCCCGTCCATTGGCATCCGGCATCACCCCAA
CAACCGAAAATCCACACTTT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MSQSPIQNPTSDPNAQSVQETSESKYGERQIAEGTLITFPNPRVGRRYDIHITLPEFTCKCPFSGYPDFATIHLTYVPDQ
RVVELKALKLYINSYRDRYISHEESVNQILDDIVAACDPLEITVKGDFLPRGNVHTVIEVHHQK

Sequences:

>Translated_144_residues
MSQSPIQNPTSDPNAQSVQETSESKYGERQIAEGTLITFPNPRVGRRYDIHITLPEFTCKCPFSGYPDFATIHLTYVPDQ
RVVELKALKLYINSYRDRYISHEESVNQILDDIVAACDPLEITVKGDFLPRGNVHTVIEVHHQK
>Mature_143_residues
SQSPIQNPTSDPNAQSVQETSESKYGERQIAEGTLITFPNPRVGRRYDIHITLPEFTCKCPFSGYPDFATIHLTYVPDQR
VVELKALKLYINSYRDRYISHEESVNQILDDIVAACDPLEITVKGDFLPRGNVHTVIEVHHQK

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_ACAM1 (B0CDX9)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518648.1
- ProteinModelPortal:   B0CDX9
- SMR:   B0CDX9
- GeneID:   5683155
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4352
- HOGENOM:   HBG294103
- OMA:   HEESANQ
- ProtClustDB:   PRK13258
- BioCyc:   AMAR329726:AM1_4352-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 16401; Mature: 16269

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSPIQNPTSDPNAQSVQETSESKYGERQIAEGTLITFPNPRVGRRYDIHITLPEFTCK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCEEEE
CPFSGYPDFATIHLTYVPDQRVVELKALKLYINSYRDRYISHEESVNQILDDIVAACDPL
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCE
EITVKGDFLPRGNVHTVIEVHHQK
EEEEECCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
SQSPIQNPTSDPNAQSVQETSESKYGERQIAEGTLITFPNPRVGRRYDIHITLPEFTCK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCEEEE
CPFSGYPDFATIHLTYVPDQRVVELKALKLYINSYRDRYISHEESVNQILDDIVAACDPL
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCE
EITVKGDFLPRGNVHTVIEVHHQK
EEEEECCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA