Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 158337251

GI number: 158337251

Start: 4164310

End: 4166478

Strand: Reverse

Name: slt [H]

Synonym: AM1_4128

Alternate gene names: 158337251

Gene position: 4166478-4164310 (Counterclockwise)

Preceding gene: 158337253

Following gene: 158337248

Centisome position: 64.06

GC content: 53.39

Gene sequence:

>2169_bases
ATGGGCAGTTCTCCCAATTTTTTGAAGCGCTTAGCCCCCTGGATCATCTCCATGGGACTCAGCAGTGCCCTCGTATTAGG
GTTAGCCTGGCTATTTCTGAAACCGAAGCTGGAATCCACGGATCAGCCCTTACCATCGCTACAGGTTTCAACCGTCAATT
CTGCCCTAGTGGCATTACGGCCTCTCCCTGCCCAACAGCGGATAGAGCCTCTTAAACAGCTGGCCCAGCAGGGCACTGCC
CAAGAACAGAATCAGGCCCGGTATGTGTTAACTGCGGACCTCATTAACCTGGGCCAAGGGGCAGAAGCCCTGCAGTGGTT
AGAGGGGCTGGAAGCGCGTTACCCTCTATTAGGGAGCCATATCGCCGTTCTCCAGGCTGAGGCCCAAACCCTAGCGGGGC
AGACAGAGCAAGCCACCAAGACCTGGCAGCGCATTATTGCCGACTATCCCCAAGAACCCGCTGCAGCAGAAGCTTTATTT
GTACTCGGCCAGCAAAAGCCTGAACTCTGGCAGCAAGCTATCGCCGATTTCCCCGCCCATCCTAAGACGGTGAAGATTGC
TGTAGAGCAGTTAAAGAAAAAGCCCAAGCAGTTACCGCTACTGATGCTCATTGCAGAGCATGGTTTATTTTTAACCAATT
ATGGAGATTACTTAACCCAGCTGACGAAGGAATTCAAAGGCCAGCTCAAACCTGCTGATTGGCAAATTATCGCCTTTGGA
TACTGGGAAAAACAACTGTATAAGGAGGGGGCACTGGCCTATGCCCAAGCCCCCCAGACGTCCCAAACGGCTTATCGAGG
TGCACGGGGCTTGCAGCTCGGGGGAGAAAAGAAACAAGCGAGGGCTGCCTATCAAAAAATGATCGCGGCCTATCCTCAGG
CCAAAGAAACGCCCCTGGCCTTGAGCAAGCTCGCCAGTCTAGAACCAGACTTGGGCAAAGCAGCCCAGCACTATGACCGC
GCCTTTCAACTAAGTAAAGACCAGCAGCCCACCTTAGCTGCCCAAATCCTCGGGGAGCGAATTCAGCGGTTAAAGAAGGC
CAAACAGCTATCTTCCACCTTTGAGAAAACGTTGCTGCAACAGTTTTCCGATTCAAACGATGCAGCGACTCTGCGATGGC
AGCAGGCAGAGCAGCAGCGAAAGGCAGGGAATCTGGCTGGTGCCCGCAAGTTGGCCCTGGAAATCCAAAAGCAAAACCCC
AATAGCCCCCAGGCCGCAACTGCCGTGTTCTGGGCCGGGAAATGGGCCTCCACGGCAGAAGAGCGGCAAGCAGCCTTTCA
AACCCTCTGGCAAACCTATCCTGATTCCTACTATGCCTGGCGAGCGGCTGTACTATCGGGCTGGAACGTTGGCGATTTTA
CGTCCATCCGTTCCTTCCAACCCCAAATTCGGTTTCCCAGTACGCAACTGCCTTTGGCCGCCGGATCCCCAGCTCTGAAA
GAGCTATACCAGCTCGGACAAAGCAAAACGGCCTGGGCACGCTGGCAATGGGAGTTTCGCAATCGCCAGCAGCCTAGCTT
ATCGGAACAGCTCACGGATGGCTTATTGCGCCTCGGGGTTCAGGAATACCTAGACGGCATTTATATGCTGGCCAATCTGG
AAATGCGGGCACGGGAGGAAAACGATCCCGAAAAGCTAGTCGAACAGTGGCAAGACCATCCGGGGTTTGAACAGGCCCTA
TTTCCCTTGGCCTACTTCACCCCCATTCGCAAATGGTCCAACCAGCGCAATCTGAATCCGTTTCTGGTGTTAAGCCTGAT
GCGTCAGGAATCACGGTTTCAGCCCAAAATTCGCTCCGTCGCGGGAGCCGTAGGGCTGATGCAGGTGCTGCCAGAAACCG
CTGAATGGGTGAGTGAAAAAGCAGGTCTCAAAGACTATCAAATCGACGGCATTGACGACAATATCAATCTCGGCACCTGG
TACCTGGACTATACCCACCGCAACTATAACGACAATTCCATGCTTGCGATCGCAAGCTATAATGCTGGCCCTGGTAATGT
AGATCGGTGGGTTAAACAGACTTCGGTGACGGATGTCGATGTCTTTGTCGAAGAGATCCCCTTCCCAGAGACGAAAAACT
ATGTCAAATCCGTCTCTGAGAACTATTGGAATTATCTCAGGCTCTACAACCCCCAAATCCAACAGCTAATGCAAGAGCAG
GCCCAGTAG

Upstream 100 bases:

>100_bases
TTGAAGGATGAGTCATTAAACGCAACGGACCCATTACGGGTTCTCGAATTATTTTCTAGAATGCAATGAGTTTTCCTATT
TTTTAACCTTTTTCAAACCC

Downstream 100 bases:

>100_bases
GTCTGCTCTGTACAAGCTGCAAGCTGGCGTTGAAGATGCCCCTGTCTGTTCTGTTACCTTTGAGCCATGATGGCTCGATG
GATCAGTTGTTCAACCGAAG

Product: Slt family transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MGSSPNFLKRLAPWIISMGLSSALVLGLAWLFLKPKLESTDQPLPSLQVSTVNSALVALRPLPAQQRIEPLKQLAQQGTA
QEQNQARYVLTADLINLGQGAEALQWLEGLEARYPLLGSHIAVLQAEAQTLAGQTEQATKTWQRIIADYPQEPAAAEALF
VLGQQKPELWQQAIADFPAHPKTVKIAVEQLKKKPKQLPLLMLIAEHGLFLTNYGDYLTQLTKEFKGQLKPADWQIIAFG
YWEKQLYKEGALAYAQAPQTSQTAYRGARGLQLGGEKKQARAAYQKMIAAYPQAKETPLALSKLASLEPDLGKAAQHYDR
AFQLSKDQQPTLAAQILGERIQRLKKAKQLSSTFEKTLLQQFSDSNDAATLRWQQAEQQRKAGNLAGARKLALEIQKQNP
NSPQAATAVFWAGKWASTAEERQAAFQTLWQTYPDSYYAWRAAVLSGWNVGDFTSIRSFQPQIRFPSTQLPLAAGSPALK
ELYQLGQSKTAWARWQWEFRNRQQPSLSEQLTDGLLRLGVQEYLDGIYMLANLEMRAREENDPEKLVEQWQDHPGFEQAL
FPLAYFTPIRKWSNQRNLNPFLVLSLMRQESRFQPKIRSVAGAVGLMQVLPETAEWVSEKAGLKDYQIDGIDDNINLGTW
YLDYTHRNYNDNSMLAIASYNAGPGNVDRWVKQTSVTDVDVFVEEIPFPETKNYVKSVSENYWNYLRLYNPQIQQLMQEQ
AQ

Sequences:

>Translated_722_residues
MGSSPNFLKRLAPWIISMGLSSALVLGLAWLFLKPKLESTDQPLPSLQVSTVNSALVALRPLPAQQRIEPLKQLAQQGTA
QEQNQARYVLTADLINLGQGAEALQWLEGLEARYPLLGSHIAVLQAEAQTLAGQTEQATKTWQRIIADYPQEPAAAEALF
VLGQQKPELWQQAIADFPAHPKTVKIAVEQLKKKPKQLPLLMLIAEHGLFLTNYGDYLTQLTKEFKGQLKPADWQIIAFG
YWEKQLYKEGALAYAQAPQTSQTAYRGARGLQLGGEKKQARAAYQKMIAAYPQAKETPLALSKLASLEPDLGKAAQHYDR
AFQLSKDQQPTLAAQILGERIQRLKKAKQLSSTFEKTLLQQFSDSNDAATLRWQQAEQQRKAGNLAGARKLALEIQKQNP
NSPQAATAVFWAGKWASTAEERQAAFQTLWQTYPDSYYAWRAAVLSGWNVGDFTSIRSFQPQIRFPSTQLPLAAGSPALK
ELYQLGQSKTAWARWQWEFRNRQQPSLSEQLTDGLLRLGVQEYLDGIYMLANLEMRAREENDPEKLVEQWQDHPGFEQAL
FPLAYFTPIRKWSNQRNLNPFLVLSLMRQESRFQPKIRSVAGAVGLMQVLPETAEWVSEKAGLKDYQIDGIDDNINLGTW
YLDYTHRNYNDNSMLAIASYNAGPGNVDRWVKQTSVTDVDVFVEEIPFPETKNYVKSVSENYWNYLRLYNPQIQQLMQEQ
AQ
>Mature_721_residues
GSSPNFLKRLAPWIISMGLSSALVLGLAWLFLKPKLESTDQPLPSLQVSTVNSALVALRPLPAQQRIEPLKQLAQQGTAQ
EQNQARYVLTADLINLGQGAEALQWLEGLEARYPLLGSHIAVLQAEAQTLAGQTEQATKTWQRIIADYPQEPAAAEALFV
LGQQKPELWQQAIADFPAHPKTVKIAVEQLKKKPKQLPLLMLIAEHGLFLTNYGDYLTQLTKEFKGQLKPADWQIIAFGY
WEKQLYKEGALAYAQAPQTSQTAYRGARGLQLGGEKKQARAAYQKMIAAYPQAKETPLALSKLASLEPDLGKAAQHYDRA
FQLSKDQQPTLAAQILGERIQRLKKAKQLSSTFEKTLLQQFSDSNDAATLRWQQAEQQRKAGNLAGARKLALEIQKQNPN
SPQAATAVFWAGKWASTAEERQAAFQTLWQTYPDSYYAWRAAVLSGWNVGDFTSIRSFQPQIRFPSTQLPLAAGSPALKE
LYQLGQSKTAWARWQWEFRNRQQPSLSEQLTDGLLRLGVQEYLDGIYMLANLEMRAREENDPEKLVEQWQDHPGFEQALF
PLAYFTPIRKWSNQRNLNPFLVLSLMRQESRFQPKIRSVAGAVGLMQVLPETAEWVSEKAGLKDYQIDGIDDNINLGTWY
LDYTHRNYNDNSMLAIASYNAGPGNVDRWVKQTSVTDVDVFVEEIPFPETKNYVKSVSENYWNYLRLYNPQIQQLMQEQA
Q

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=153, Percent_Identity=36.6013071895425, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 81617; Mature: 81485

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSPNFLKRLAPWIISMGLSSALVLGLAWLFLKPKLESTDQPLPSLQVSTVNSALVALR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHC
PLPAQQRIEPLKQLAQQGTAQEQNQARYVLTADLINLGQGAEALQWLEGLEARYPLLGSH
CCCHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHCCCHHHCH
IAVLQAEAQTLAGQTEQATKTWQRIIADYPQEPAAAEALFVLGQQKPELWQQAIADFPAH
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCC
PKTVKIAVEQLKKKPKQLPLLMLIAEHGLFLTNYGDYLTQLTKEFKGQLKPADWQIIAFG
CCHHHHHHHHHHHCHHHCCHHHHHHHCCEEEECHHHHHHHHHHHHCCCCCCCCEEEEEEE
YWEKQLYKEGALAYAQAPQTSQTAYRGARGLQLGGEKKQARAAYQKMIAAYPQAKETPLA
HHHHHHHHHCCHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
LSKLASLEPDLGKAAQHYDRAFQLSKDQQPTLAAQILGERIQRLKKAKQLSSTFEKTLLQ
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFSDSNDAATLRWQQAEQQRKAGNLAGARKLALEIQKQNPNSPQAATAVFWAGKWASTAE
HHCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCCHHHEEEECCCCCCHHH
ERQAAFQTLWQTYPDSYYAWRAAVLSGWNVGDFTSIRSFQPQIRFPSTQLPLAAGSPALK
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHH
ELYQLGQSKTAWARWQWEFRNRQQPSLSEQLTDGLLRLGVQEYLDGIYMLANLEMRAREE
HHHHCCCCCCHHHEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
NDPEKLVEQWQDHPGFEQALFPLAYFTPIRKWSNQRNLNPFLVLSLMRQESRFQPKIRSV
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHH
AGAVGLMQVLPETAEWVSEKAGLKDYQIDGIDDNINLGTWYLDYTHRNYNDNSMLAIASY
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEEEC
NAGPGNVDRWVKQTSVTDVDVFVEEIPFPETKNYVKSVSENYWNYLRLYNPQIQQLMQEQ
CCCCCCHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AQ
CC
>Mature Secondary Structure 
GSSPNFLKRLAPWIISMGLSSALVLGLAWLFLKPKLESTDQPLPSLQVSTVNSALVALR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHC
PLPAQQRIEPLKQLAQQGTAQEQNQARYVLTADLINLGQGAEALQWLEGLEARYPLLGSH
CCCHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHCCCHHHCH
IAVLQAEAQTLAGQTEQATKTWQRIIADYPQEPAAAEALFVLGQQKPELWQQAIADFPAH
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCC
PKTVKIAVEQLKKKPKQLPLLMLIAEHGLFLTNYGDYLTQLTKEFKGQLKPADWQIIAFG
CCHHHHHHHHHHHCHHHCCHHHHHHHCCEEEECHHHHHHHHHHHHCCCCCCCCEEEEEEE
YWEKQLYKEGALAYAQAPQTSQTAYRGARGLQLGGEKKQARAAYQKMIAAYPQAKETPLA
HHHHHHHHHCCHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
LSKLASLEPDLGKAAQHYDRAFQLSKDQQPTLAAQILGERIQRLKKAKQLSSTFEKTLLQ
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFSDSNDAATLRWQQAEQQRKAGNLAGARKLALEIQKQNPNSPQAATAVFWAGKWASTAE
HHCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHEEEECCCCCCCCHHHEEEECCCCCCHHH
ERQAAFQTLWQTYPDSYYAWRAAVLSGWNVGDFTSIRSFQPQIRFPSTQLPLAAGSPALK
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHH
ELYQLGQSKTAWARWQWEFRNRQQPSLSEQLTDGLLRLGVQEYLDGIYMLANLEMRAREE
HHHHCCCCCCHHHEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
NDPEKLVEQWQDHPGFEQALFPLAYFTPIRKWSNQRNLNPFLVLSLMRQESRFQPKIRSV
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHH
AGAVGLMQVLPETAEWVSEKAGLKDYQIDGIDDNINLGTWYLDYTHRNYNDNSMLAIASY
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEEEC
NAGPGNVDRWVKQTSVTDVDVFVEEIPFPETKNYVKSVSENYWNYLRLYNPQIQQLMQEQ
CCCCCCHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]