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The map label for this gene is rnhB

Identifier: 158337229

GI number: 158337229

Start: 4143526

End: 4144122

Strand: Direct

Name: rnhB

Synonym: AM1_4107

Alternate gene names: 158337229

Gene position: 4143526-4144122 (Clockwise)

Preceding gene: 158337228

Following gene: 158337233

Centisome position: 63.71

GC content: 50.92

Gene sequence:

>597_bases
ATGGATGATGCCTCTGAGCAGATTGCAGGTGTTGATGAAGTCGGTCGAGGGGCCTTGTTTGGCCCTGTTGTAGCGGCTAC
CGTTATCCTCTCTGATGGGGCCATTGAACAGTTAGTGGCTCAAGGGATGACGGATAGTAAGAAACTATCGCCACAGCGTC
GAATGATATTGATGAACCAAATCCGAGAGGTGGCAACGGGGTTTAGGGTGGGGATGGCGTCAGTATATGAGATTGATCGC
CTCAACATTTTGCAGGCCAGTTTACTGGCCATGCGGCGGGCTGTGCTGGGTTTACCATCCACTCCTCAGTTATGCTTAGT
CGATGGAAACCAACGGATTCCGAATTTACCGGTACCGCAAAGAACGGTCGTTAAAGGCGATCAATCCGAACCTGAGATCG
CAGCTGCAAGCATCCTGGCTAAAGTCTGGCGTGATCAACTGATTGTCCGTTTAGATCAGCGTTACCCGGGGTATGATCTA
GCATCGAATAAGGGCTATGGCAGTGCTAAACATCGGCAAGCGTTGCGAGAACTAGGACCAACGCGCCAACATCGATTGTC
CTTTGCCCCTTGTCAGGCCAGTTTACTTCCAGATTAA

Upstream 100 bases:

>100_bases
TCTTGGTATTGCCTCGATCCTGGTCCTAGAACTGGAAATCAACCCAGGGCTGATCAGCTTTGACCCTATAGTGGTGTGGG
GACTCGTATGCATGTGAAGC

Downstream 100 bases:

>100_bases
GGATCTGAGGTATCAGATTTTTTGAGCCATTTTTGGTACAAATCCGGTCTGCGATCTCGAGTTCGTTGGATCTGCTGTTC
CTGTCGCCACCGCTCTATCA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MDDASEQIAGVDEVGRGALFGPVVAATVILSDGAIEQLVAQGMTDSKKLSPQRRMILMNQIREVATGFRVGMASVYEIDR
LNILQASLLAMRRAVLGLPSTPQLCLVDGNQRIPNLPVPQRTVVKGDQSEPEIAAASILAKVWRDQLIVRLDQRYPGYDL
ASNKGYGSAKHRQALRELGPTRQHRLSFAPCQASLLPD

Sequences:

>Translated_198_residues
MDDASEQIAGVDEVGRGALFGPVVAATVILSDGAIEQLVAQGMTDSKKLSPQRRMILMNQIREVATGFRVGMASVYEIDR
LNILQASLLAMRRAVLGLPSTPQLCLVDGNQRIPNLPVPQRTVVKGDQSEPEIAAASILAKVWRDQLIVRLDQRYPGYDL
ASNKGYGSAKHRQALRELGPTRQHRLSFAPCQASLLPD
>Mature_198_residues
MDDASEQIAGVDEVGRGALFGPVVAATVILSDGAIEQLVAQGMTDSKKLSPQRRMILMNQIREVATGFRVGMASVYEIDR
LNILQASLLAMRRAVLGLPSTPQLCLVDGNQRIPNLPVPQRTVVKGDQSEPEIAAASILAKVWRDQLIVRLDQRYPGYDL
ASNKGYGSAKHRQALRELGPTRQHRLSFAPCQASLLPD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=191, Percent_Identity=50.7853403141361, Blast_Score=173, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_ACAM1 (B0CAM1)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518404.1
- ProteinModelPortal:   B0CAM1
- SMR:   B0CAM1
- GeneID:   5682910
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4107
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK13925
- BioCyc:   AMAR329726:AM1_4107-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21583; Mature: 21583

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDASEQIAGVDEVGRGALFGPVVAATVILSDGAIEQLVAQGMTDSKKLSPQRRMILMNQ
CCCHHHHHHCHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHCCHHHHHHHHHH
IREVATGFRVGMASVYEIDRLNILQASLLAMRRAVLGLPSTPQLCLVDGNQRIPNLPVPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
RTVVKGDQSEPEIAAASILAKVWRDQLIVRLDQRYPGYDLASNKGYGSAKHRQALRELGP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
TRQHRLSFAPCQASLLPD
CHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDDASEQIAGVDEVGRGALFGPVVAATVILSDGAIEQLVAQGMTDSKKLSPQRRMILMNQ
CCCHHHHHHCHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHCCHHHHHHHHHH
IREVATGFRVGMASVYEIDRLNILQASLLAMRRAVLGLPSTPQLCLVDGNQRIPNLPVPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCC
RTVVKGDQSEPEIAAASILAKVWRDQLIVRLDQRYPGYDLASNKGYGSAKHRQALRELGP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
TRQHRLSFAPCQASLLPD
CHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA