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The map label for this gene is insG [H]

Identifier: 158337202

GI number: 158337202

Start: 4127369

End: 4128595

Strand: Reverse

Name: insG [H]

Synonym: AM1_4079

Alternate gene names: 158337202

Gene position: 4128595-4127369 (Counterclockwise)

Preceding gene: 158337203

Following gene: 158337200

Centisome position: 63.48

GC content: 50.45

Gene sequence:

>1227_bases
TTGATAGATATAGGATCAAGACTTGCACGCCATAAACTTCTGCCTTTCACTATGTCTGAGTCAGCCGAAATTCTCAAGCA
GCAATTTTCCCAAAGCCTGGGTTTACCGTGGACAGATATCCTTCTAGCCTCAAGGTTAGAGGAACTCCTGAAAGAAGAAG
CATTCTCCTATCGCAACCGGATATATAGCCCCATCGTTACGCTGTGGGCCATGCTTTACCAAGTGCTATCAGCCGATAAA
AGCCTTCGTAACACAGTCAAATGCATCACGACTTGGCTCACAGCGGCTGGCATCCAACCGCCCTCATCTGATACCGGAGC
CTACAGCAAAGCCAGAAGTCGCTTTCCAGAGTCACTGTTGCAACGTTTAATTCCCGAAAGCGCTGAGTGCTTAGCGCAAC
CCCTCTCCCCAGAGCACCTCTGGTGTGGTCGGCCCGTCAAGGTCTACGACGGAACCACCGTGTTGATGGCTGATAGTGCG
GCCAACCAAGCATCATATCCACAACATGGCAATCAAACAGCAGGCTGTGGTTTTCCCATCGCTCGCTTGGTAGTGTTCTT
CTGTTTGGTTACCGGTGCAGTGGCGTCAGCTTGTATTGCCTCCTGGGACACCAGTGAAATTGTCATGAGTCGTTTGCTCT
ATCAAGACCTTGAGGTCGGTGATGTGGTCATGGCGGACCAAGCTTATGGCAGCTATGTTGATCTAGCCATCATTCAACAA
CACAGGGCTGATGGAGTGTTGCGTAAACATCATGCTCGCAAGACTGATTTCCGCAAAGGCAACAAGCATGGCATTGGTGA
TCATCAGGTGACATGGCATAAGCCAGCCCAACGGCCTGAGCACATGAGTGAGCAAGATTTTGCCCTGATTCCTCAAACAT
TGGTCGTTAGAGAGGTGTGTTTGCGCTTATCCCTTAAGGGCTTTCGCGACCAGCACATTATTGTGGTGACGACGCTGCTG
GATGCTCAACGCTACAGCGCTGGGCAACTGACTCGCTTGTATGGCTGGCGTTGGCCAGTGGCGGAAGTCAATCTGCGCCA
TCTCAAAACCACCTTAAAAATGGAGATGCTCAGTGCCAAAACTCCGGATATGGTGCGCAAGGACATTTGGGTACATTTGT
TGGGCTATAACCTACTCAGAAGTCTCATGGAACTTGCGGCACCGCTAGCAGATAATGCTAGAACTCAACTGTCTGTGCAA
GGAGCACGACAACACGAGAGTGTTTAG

Upstream 100 bases:

>100_bases
CTTGTGGATGGAGCAGCCGCCGAGATCAGGCTATGGGAAATCAAGCCCTATCTGTAGGTCAATGGCACTGACTTAAGAAG
TCAGGAAAAGGCTACAAGTC

Downstream 100 bases:

>100_bases
ATTTTTGTGTAAGCAGCAGTGTATTCAATAGTTGAATCGTGTTGGGAACAGAATAGCAAAATGGGACAGCGCCGCCTTCC
AATTCAGAATTGGTCTGGTC

Product: IS4 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILLASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADK
SLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSA
ANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVCLRLSLKGFRDQHIIVVTTLL
DAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHESV

Sequences:

>Translated_408_residues
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILLASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADK
SLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSA
ANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVCLRLSLKGFRDQHIIVVTTLL
DAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHESV
>Mature_408_residues
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILLASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADK
SLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSA
ANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVCLRLSLKGFRDQHIIVVTTLL
DAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHESV

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=304, Percent_Identity=24.3421052631579, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 45873; Mature: 45873

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILLASRLEELLKEEAFSYRNR
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IYSPIVTLWAMLYQVLSADKSLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSAANQASYPQHGNQTAGCGFPI
HHHCCCHHHHHHCCCCCCCEECCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
ARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
LRLSLKGFRDQHIIVVTTLLDAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAK
HHHHHCCCCCCEEEEEEEHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
TPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQGARQHESV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCHHCCCC
>Mature Secondary Structure
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILLASRLEELLKEEAFSYRNR
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IYSPIVTLWAMLYQVLSADKSLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSAANQASYPQHGNQTAGCGFPI
HHHCCCHHHHHHCCCCCCCEECCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
ARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
LRLSLKGFRDQHIIVVTTLLDAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAK
HHHHHCCCCCCEEEEEEEHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
TPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQGARQHESV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]