Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 158336977

GI number: 158336977

Start: 3903731

End: 3904855

Strand: Direct

Name: tgt [H]

Synonym: AM1_3850

Alternate gene names: 158336977

Gene position: 3903731-3904855 (Clockwise)

Preceding gene: 158336976

Following gene: 158336978

Centisome position: 60.02

GC content: 51.2

Gene sequence:

>1125_bases
GTGAGCAATCAGTTTCGGTTTGAATGTCATCAGCACTGCTCCCAAACCCAGGCCCGTGTTGGCACCTTTTATACCCCCCA
TGGTCCTGTGGATACGCCCCGGTTTATGCCCGTGGGCACCTTAGCCAATGTGAAAACCTTGACCCCAGCTCAACTGCAAG
AGACGGGGGCTCAAATGATTTTGGCTAATACCTATCATTTGCATTTGCAGCCCGGTGAATCCATCGTGGAGCAAGCTGGG
GGCCTACATCGATTTATGAGCTGGTCAGGTCCTATATTGACGGATTCCGGCGGTTTTCAAGTCTTTAGCCTTAGTGACAT
GCGTTCCATCGCCGAAGAGGGGGTGAGTTTTCGGTCTCCCCGGGATGGTCAAATGATTTATCTTTCGCCCGAAACTTCCA
TGGCGATTCAAAATGCCCTGGGAGCGGATGTGATCATGGCCTTTGATGAATGTCCCTCCTATCCGGCGAGTCGAGACGCG
ATTCAAGCCGCCACAGAGCGAACCCAGCGCTGGCTAGCCCGTTGCGTTAAGGCGCACGACCGCTCTGATCAGGCTCTATT
TGGCATTGTTCAGGGGGGAACCTTTGCCGATTTGCGTCAAGCTGCCGCGCGATCTGTGGTGGAATTTGATTTACCCGGCT
ATGCCATTGGCGGTGTCAGTGTGGGAGAACCCCCGGAACTTATTGAAAAAATTGTCAAAGTGACAACGCCCTTGTTACCG
GTCCATAAACCCCGGTATCTCATGGGGGTGGGCACCTACCGAGAGATGGTGCAAGCCATTGCTGCCGGGATTGATTTGTT
TGACTGTGTGATTCCGACGCGCTTAGCCCGTCATGGCAATGCTTTAGTCGCCGGAGAACGATGGAATCTCAAGAACAATC
GATTCCGGGAAGACTTTACTCCCCTGGATTCTGCCTGTCCTTGCTATACCTGCCAAAATTTTACGCGGGCCTATATCAGT
CATTTGTTACGTTCACGAGAACTTCTCGCCTATACCCTCTTAAGTATTCACAATATTACGGAGCTAATTGGCTTTACCCA
GCGTATTCGGCAGGCAATCCTAGATGATCAGTTTCAGCAAATCTTTGGAACATGGCTGTTTTCTTCGGATACTCAGCTCG
ATTAA

Upstream 100 bases:

>100_bases
CTTCTTGAGCGAGAACGCCGGTATTTTTTTGTCTTAACGTTCTGAGCATTCAGGATAAACCTGATATTTTGACAGAGGCC
ATCGCGATATTGAGGTTGTT

Downstream 100 bases:

>100_bases
TCGCTCCTCAATCCGCGGCTAAGGGTGATGTTTGGTAGCTCATACTAACGGCTAGGATAGAGACGATGCAGGTGGATGCC
TTACATGCCCTTAGCCGCCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSNQFRFECHQHCSQTQARVGTFYTPHGPVDTPRFMPVGTLANVKTLTPAQLQETGAQMILANTYHLHLQPGESIVEQAG
GLHRFMSWSGPILTDSGGFQVFSLSDMRSIAEEGVSFRSPRDGQMIYLSPETSMAIQNALGADVIMAFDECPSYPASRDA
IQAATERTQRWLARCVKAHDRSDQALFGIVQGGTFADLRQAAARSVVEFDLPGYAIGGVSVGEPPELIEKIVKVTTPLLP
VHKPRYLMGVGTYREMVQAIAAGIDLFDCVIPTRLARHGNALVAGERWNLKNNRFREDFTPLDSACPCYTCQNFTRAYIS
HLLRSRELLAYTLLSIHNITELIGFTQRIRQAILDDQFQQIFGTWLFSSDTQLD

Sequences:

>Translated_374_residues
MSNQFRFECHQHCSQTQARVGTFYTPHGPVDTPRFMPVGTLANVKTLTPAQLQETGAQMILANTYHLHLQPGESIVEQAG
GLHRFMSWSGPILTDSGGFQVFSLSDMRSIAEEGVSFRSPRDGQMIYLSPETSMAIQNALGADVIMAFDECPSYPASRDA
IQAATERTQRWLARCVKAHDRSDQALFGIVQGGTFADLRQAAARSVVEFDLPGYAIGGVSVGEPPELIEKIVKVTTPLLP
VHKPRYLMGVGTYREMVQAIAAGIDLFDCVIPTRLARHGNALVAGERWNLKNNRFREDFTPLDSACPCYTCQNFTRAYIS
HLLRSRELLAYTLLSIHNITELIGFTQRIRQAILDDQFQQIFGTWLFSSDTQLD
>Mature_373_residues
SNQFRFECHQHCSQTQARVGTFYTPHGPVDTPRFMPVGTLANVKTLTPAQLQETGAQMILANTYHLHLQPGESIVEQAGG
LHRFMSWSGPILTDSGGFQVFSLSDMRSIAEEGVSFRSPRDGQMIYLSPETSMAIQNALGADVIMAFDECPSYPASRDAI
QAATERTQRWLARCVKAHDRSDQALFGIVQGGTFADLRQAAARSVVEFDLPGYAIGGVSVGEPPELIEKIVKVTTPLLPV
HKPRYLMGVGTYREMVQAIAAGIDLFDCVIPTRLARHGNALVAGERWNLKNNRFREDFTPLDSACPCYTCQNFTRAYISH
LLRSRELLAYTLLSIHNITELIGFTQRIRQAILDDQFQQIFGTWLFSSDTQLD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=348, Percent_Identity=46.264367816092, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI13375872, Length=279, Percent_Identity=27.9569892473118, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1786607, Length=348, Percent_Identity=45.9770114942529, Blast_Score=338, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17544680, Length=352, Percent_Identity=43.75, Blast_Score=307, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI25146502, Length=212, Percent_Identity=30.188679245283, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920510, Length=356, Percent_Identity=44.9438202247191, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI21355311, Length=254, Percent_Identity=27.5590551181102, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41635; Mature: 41504

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQFRFECHQHCSQTQARVGTFYTPHGPVDTPRFMPVGTLANVKTLTPAQLQETGAQMI
CCCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEE
LANTYHLHLQPGESIVEQAGGLHRFMSWSGPILTDSGGFQVFSLSDMRSIAEEGVSFRSP
EEEEEEEEECCCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCCCCCC
RDGQMIYLSPETSMAIQNALGADVIMAFDECPSYPASRDAIQAATERTQRWLARCVKAHD
CCCCEEEECCCHHHHHHHHCCHHHEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RSDQALFGIVQGGTFADLRQAAARSVVEFDLPGYAIGGVSVGEPPELIEKIVKVTTPLLP
CCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCC
VHKPRYLMGVGTYREMVQAIAAGIDLFDCVIPTRLARHGNALVAGERWNLKNNRFREDFT
CCCCCCEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
PLDSACPCYTCQNFTRAYISHLLRSRELLAYTLLSIHNITELIGFTQRIRQAILDDQFQQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFGTWLFSSDTQLD
HHHHHHCCCCCCCC
>Mature Secondary Structure 
SNQFRFECHQHCSQTQARVGTFYTPHGPVDTPRFMPVGTLANVKTLTPAQLQETGAQMI
CCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEE
LANTYHLHLQPGESIVEQAGGLHRFMSWSGPILTDSGGFQVFSLSDMRSIAEEGVSFRSP
EEEEEEEEECCCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCCCCCC
RDGQMIYLSPETSMAIQNALGADVIMAFDECPSYPASRDAIQAATERTQRWLARCVKAHD
CCCCEEEECCCHHHHHHHHCCHHHEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RSDQALFGIVQGGTFADLRQAAARSVVEFDLPGYAIGGVSVGEPPELIEKIVKVTTPLLP
CCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCC
VHKPRYLMGVGTYREMVQAIAAGIDLFDCVIPTRLARHGNALVAGERWNLKNNRFREDFT
CCCCCCEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
PLDSACPCYTCQNFTRAYISHLLRSRELLAYTLLSIHNITELIGFTQRIRQAILDDQFQQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFGTWLFSSDTQLD
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA