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The map label for this gene is nylB' [H]

Identifier: 158336949

GI number: 158336949

Start: 3869241

End: 3870179

Strand: Direct

Name: nylB' [H]

Synonym: AM1_3820

Alternate gene names: 158336949

Gene position: 3869241-3870179 (Clockwise)

Preceding gene: 158336944

Following gene: 158336951

Centisome position: 59.49

GC content: 48.24

Gene sequence:

>939_bases
TTGGAGAAAATTGAACAGTATGCCCAAGACCATCGCTCCTCGTCACTCTTGGTGATGCATCAAGGCAAACTGGTGCTGGA
AAAGTACTGGCGAGGTCATAACCAGGCGGCTTATACCAATTCCATGTCCATGGCCAAAACGGTCTTGTCTTTGCTGATTG
GCATTGCGATCGCAGACGGTAGCATCGGTTCAGAACAGGACCCAGTTGCCAAATATATTCCTGAATGGGCAAACGATGAT
CGGGCCAAAATTACGATTGAAGATTTGCTGAAAATGCAGTCTGGCCTTCGGGATTATGAAATCGCTGACGATCCCTTTTC
GGATATTATCAAGCTCTATTTAGGTACTGATGCTCAATCGGCGGCTTTAAACGTTCCGGCAGAGCGCCCCTCCGGCCAGG
AGTTTGCTTATATCAATGCCAATAGCCAAATTTTAGGTCTGCTTTTGGAGCGAGCGACGGGGCAAGGATATGGTCAATAC
TTATCTACCAAGCTTTGGCAGCCTATTGGTGCTCAAAAAGCGCAGCTATGGTTAGATCGACCGGACGGTAATGCGAAACC
CTTTTGCTGTTTGTTTGCGCGATCGCAGGATTGGCTACGGGTGGGTCAACTCTTGCTCAATAATGGCAAGGTGAATCAAA
AACAGGTGGTTCCTGCAGACTGGATTCAAAAGATGTTGGTCTCTAGTGGATCAGAACCTGCCTATGGCTACCATATTTGG
CTAAAGGCGCGCACCCAAGATGGACCAGGCTATAAAAAAAGAGCCACAAAGCCCTTTTTGGCGGAGGATATGTTTTATCT
AGATGGGTGGGGACAACAGCGGGTCTATGTGATGCCGTCTCAGGAGTTGGTGGTGGTTCGAGTCGGAGAAAAACCCAAAA
CTTGGGATGATGCGGTGATTCCCAATACTCTCATTCGTGGCTTGCAGTCTGCAAAGTAG

Upstream 100 bases:

>100_bases
TCCCATTACAGGGGTGGGGTGGTACGCTCCCATGGCGACGGTTTCTGGGGGAGATAGTCAACCTGTTGCCACGGCTCAAC
CCCAGTTGTCTCAGGCTGCC

Downstream 100 bases:

>100_bases
AAGGGGATCTCCAATATGTGGTGGGTTAGGTCTTGGACTGTAGAAAGTTGACAATATCTTGATGGTCTAAAGATTCCGCA
ATTGTCAAAGCGGTATCACC

Product: beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII' [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MEKIEQYAQDHRSSSLLVMHQGKLVLEKYWRGHNQAAYTNSMSMAKTVLSLLIGIAIADGSIGSEQDPVAKYIPEWANDD
RAKITIEDLLKMQSGLRDYEIADDPFSDIIKLYLGTDAQSAALNVPAERPSGQEFAYINANSQILGLLLERATGQGYGQY
LSTKLWQPIGAQKAQLWLDRPDGNAKPFCCLFARSQDWLRVGQLLLNNGKVNQKQVVPADWIQKMLVSSGSEPAYGYHIW
LKARTQDGPGYKKRATKPFLAEDMFYLDGWGQQRVYVMPSQELVVVRVGEKPKTWDDAVIPNTLIRGLQSAK

Sequences:

>Translated_312_residues
MEKIEQYAQDHRSSSLLVMHQGKLVLEKYWRGHNQAAYTNSMSMAKTVLSLLIGIAIADGSIGSEQDPVAKYIPEWANDD
RAKITIEDLLKMQSGLRDYEIADDPFSDIIKLYLGTDAQSAALNVPAERPSGQEFAYINANSQILGLLLERATGQGYGQY
LSTKLWQPIGAQKAQLWLDRPDGNAKPFCCLFARSQDWLRVGQLLLNNGKVNQKQVVPADWIQKMLVSSGSEPAYGYHIW
LKARTQDGPGYKKRATKPFLAEDMFYLDGWGQQRVYVMPSQELVVVRVGEKPKTWDDAVIPNTLIRGLQSAK
>Mature_312_residues
MEKIEQYAQDHRSSSLLVMHQGKLVLEKYWRGHNQAAYTNSMSMAKTVLSLLIGIAIADGSIGSEQDPVAKYIPEWANDD
RAKITIEDLLKMQSGLRDYEIADDPFSDIIKLYLGTDAQSAALNVPAERPSGQEFAYINANSQILGLLLERATGQGYGQY
LSTKLWQPIGAQKAQLWLDRPDGNAKPFCCLFARSQDWLRVGQLLLNNGKVNQKQVVPADWIQKMLVSSGSEPAYGYHIW
LKARTQDGPGYKKRATKPFLAEDMFYLDGWGQQRVYVMPSQELVVVRVGEKPKTWDDAVIPNTLIRGLQSAK

Specific function: This Protein Is A Serine Beta-Lactamase With A Substrate Specificity For Cephalosporins. [C]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 35009; Mature: 35009

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIEQYAQDHRSSSLLVMHQGKLVLEKYWRGHNQAAYTNSMSMAKTVLSLLIGIAIADG
CHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECC
SIGSEQDPVAKYIPEWANDDRAKITIEDLLKMQSGLRDYEIADDPFSDIIKLYLGTDAQS
CCCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
AALNVPAERPSGQEFAYINANSQILGLLLERATGQGYGQYLSTKLWQPIGAQKAQLWLDR
CEEECCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEC
PDGNAKPFCCLFARSQDWLRVGQLLLNNGKVNQKQVVPADWIQKMLVSSGSEPAYGYHIW
CCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
LKARTQDGPGYKKRATKPFLAEDMFYLDGWGQQRVYVMPSQELVVVRVGEKPKTWDDAVI
EEEECCCCCCHHHHCCCCHHHHCCEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCCCC
PNTLIRGLQSAK
HHHHHHHHHCCC
>Mature Secondary Structure
MEKIEQYAQDHRSSSLLVMHQGKLVLEKYWRGHNQAAYTNSMSMAKTVLSLLIGIAIADG
CHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECC
SIGSEQDPVAKYIPEWANDDRAKITIEDLLKMQSGLRDYEIADDPFSDIIKLYLGTDAQS
CCCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
AALNVPAERPSGQEFAYINANSQILGLLLERATGQGYGQYLSTKLWQPIGAQKAQLWLDR
CEEECCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEC
PDGNAKPFCCLFARSQDWLRVGQLLLNNGKVNQKQVVPADWIQKMLVSSGSEPAYGYHIW
CCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
LKARTQDGPGYKKRATKPFLAEDMFYLDGWGQQRVYVMPSQELVVVRVGEKPKTWDDAVI
EEEECCCCCCHHHHCCCCHHHHCCEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCCCC
PNTLIRGLQSAK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 1879421 [H]