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The map label for this gene is prfB [H]

Identifier: 158336840

GI number: 158336840

Start: 3766728

End: 3767642

Strand: Reverse

Name: prfB [H]

Synonym: AM1_3708

Alternate gene names: 158336840

Gene position: 3767642-3766728 (Counterclockwise)

Preceding gene: 158336841

Following gene: 158336839

Centisome position: 57.93

GC content: 52.13

Gene sequence:

>915_bases
TTGACCCAACTGCAGGACTGGCAAGGCACCCTAGACGATGCCGAAGCCTTAGTCGAATTGCTGCAGCTCGAAGAAGATGC
AGGCCTCTTACAAGAAGCGGCAGGGAACCTAAGGCAGCTCAATCAGCAGCTCGATCAATGGGAATTACAGCAACTCTTGT
CGGGTCCCTATGACAAGGTGGGAGCTGTACTGACCATCAATACGGGAGCCGGTGGGACGGATGCCCAAGATTGGGCGGAA
ATGCTGCTGCGCATGTATACCCGCTGGTCAGAACGCCAGGGGTATAAGGTGCATCTGGCAGAACTCTCGGAAGGGGACGA
GGCTGGCATTAAATCAGCAACCCTGGAAGTGGAAGGACGCTATGCCTATGGCTATCTGAAAGCGGAAAAAGGGACGCATC
GCTTGGTCCGTATTTCTCCGTTTAACGCCAACGGCAAACGGCAAACCAGTTTTGCTGGGATAGAAGTCATGCCCCAACTC
GATCATGAGGTGGAGCTTGATATTCCCGATACGGATTTGGAAATTACCACCTCTCGATCCGGAGGTAAGGGAGGCCAGAA
CGTTAACAAAGTGGAAACGGCGGTGCGTATTGTCCATGTGCCGACGGGGTTAGCGGTGCGCTGTACCCAGGAGCGATCGC
AACTGCAAAACAAAGAAAAAGCGATGGCATTGCTGAAAGCCAAGCTGTTGGTGATTGCTCAAGAACAGAAGGCCAAAGAG
ATTGCTGATATTCGCGGTGATGCCGTGGAAGCAGCCTGGGGCAACCAAATTCGCAACTATGTGTTTCATCCCTACCAGAT
GGTGAAGGACTTAAGGACCAACCAAGAAACCACCGCTATTGAGAGTGTGATGGATGGCGCCCTAGATAACTTTATTGAGG
CGTACTTGCGTCAGGAGAATCAAATCGCTTCATGA

Upstream 100 bases:

>100_bases
AATCAACGATCTTGAACAACTCGCAGCCCAGCCAGAGTTCTGGGATGAACAGGAAAAAGCTCAGAAGGTTTTACAGGATC
TCAACGACCTTAAAGCCCAT

Downstream 100 bases:

>100_bases
TCTTAACGATTGACGAAGTGCTGACCGCCGAAGAACTCGCCGAGATGAAGGATGTGCTGGCAACGGCAGACTTTGTCGAT
GGCAAAACCACCGCAGGTTG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTQLQDWQGTLDDAEALVELLQLEEDAGLLQEAAGNLRQLNQQLDQWELQQLLSGPYDKVGAVLTINTGAGGTDAQDWAE
MLLRMYTRWSERQGYKVHLAELSEGDEAGIKSATLEVEGRYAYGYLKAEKGTHRLVRISPFNANGKRQTSFAGIEVMPQL
DHEVELDIPDTDLEITTSRSGGKGGQNVNKVETAVRIVHVPTGLAVRCTQERSQLQNKEKAMALLKAKLLVIAQEQKAKE
IADIRGDAVEAAWGNQIRNYVFHPYQMVKDLRTNQETTAIESVMDGALDNFIEAYLRQENQIAS

Sequences:

>Translated_304_residues
MTQLQDWQGTLDDAEALVELLQLEEDAGLLQEAAGNLRQLNQQLDQWELQQLLSGPYDKVGAVLTINTGAGGTDAQDWAE
MLLRMYTRWSERQGYKVHLAELSEGDEAGIKSATLEVEGRYAYGYLKAEKGTHRLVRISPFNANGKRQTSFAGIEVMPQL
DHEVELDIPDTDLEITTSRSGGKGGQNVNKVETAVRIVHVPTGLAVRCTQERSQLQNKEKAMALLKAKLLVIAQEQKAKE
IADIRGDAVEAAWGNQIRNYVFHPYQMVKDLRTNQETTAIESVMDGALDNFIEAYLRQENQIAS
>Mature_303_residues
TQLQDWQGTLDDAEALVELLQLEEDAGLLQEAAGNLRQLNQQLDQWELQQLLSGPYDKVGAVLTINTGAGGTDAQDWAEM
LLRMYTRWSERQGYKVHLAELSEGDEAGIKSATLEVEGRYAYGYLKAEKGTHRLVRISPFNANGKRQTSFAGIEVMPQLD
HEVELDIPDTDLEITTSRSGGKGGQNVNKVETAVRIVHVPTGLAVRCTQERSQLQNKEKAMALLKAKLLVIAQEQKAKEI
ADIRGDAVEAAWGNQIRNYVFHPYQMVKDLRTNQETTAIESVMDGALDNFIEAYLRQENQIAS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:
GO:0003747: Peptide chain release factor 2
GO:0005737: Peptide chain release factor 2
GO:0006412: Peptide chain release factor 2
GO:0006415: Peptide chain release factor 2
GO:0016149: Peptide chain release factor 2

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=299, Percent_Identity=32.1070234113712, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI34577120, Length=307, Percent_Identity=32.8990228013029, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI166795305, Length=204, Percent_Identity=30.8823529411765, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI2367172, Length=287, Percent_Identity=45.2961672473868, Blast_Score=269, Evalue=1e-73,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=36.8421052631579, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17542784, Length=233, Percent_Identity=32.1888412017167, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321295, Length=298, Percent_Identity=30.5369127516779, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19921226, Length=261, Percent_Identity=34.0996168582375, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 33886; Mature: 33754

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLQDWQGTLDDAEALVELLQLEEDAGLLQEAAGNLRQLNQQLDQWELQQLLSGPYDKV
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GAVLTINTGAGGTDAQDWAEMLLRMYTRWSERQGYKVHLAELSEGDEAGIKSATLEVEGR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCE
YAYGYLKAEKGTHRLVRISPFNANGKRQTSFAGIEVMPQLDHEVELDIPDTDLEITTSRS
EEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCEEEEECCC
GGKGGQNVNKVETAVRIVHVPTGLAVRCTQERSQLQNKEKAMALLKAKLLVIAQEQKAKE
CCCCCCCHHHHHHHEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHH
IADIRGDAVEAAWGNQIRNYVFHPYQMVKDLRTNQETTAIESVMDGALDNFIEAYLRQEN
HHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
QIAS
CCCC
>Mature Secondary Structure 
TQLQDWQGTLDDAEALVELLQLEEDAGLLQEAAGNLRQLNQQLDQWELQQLLSGPYDKV
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GAVLTINTGAGGTDAQDWAEMLLRMYTRWSERQGYKVHLAELSEGDEAGIKSATLEVEGR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCE
YAYGYLKAEKGTHRLVRISPFNANGKRQTSFAGIEVMPQLDHEVELDIPDTDLEITTSRS
EEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCEEEEECCC
GGKGGQNVNKVETAVRIVHVPTGLAVRCTQERSQLQNKEKAMALLKAKLLVIAQEQKAKE
CCCCCCCHHHHHHHEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHH
IADIRGDAVEAAWGNQIRNYVFHPYQMVKDLRTNQETTAIESVMDGALDNFIEAYLRQEN
HHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
QIAS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]