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The map label for this gene is ilvE [H]

Identifier: 158336778

GI number: 158336778

Start: 3702429

End: 3703343

Strand: Reverse

Name: ilvE [H]

Synonym: AM1_3646

Alternate gene names: 158336778

Gene position: 3703343-3702429 (Counterclockwise)

Preceding gene: 158336780

Following gene: 158336777

Centisome position: 56.94

GC content: 46.67

Gene sequence:

>915_bases
ATGCATAATTTTTTGCCAACTGCCTACTTTGAACATCAGTTTGTCCCTTTCAAAGAAGCGAATATCTCCATTGCCACCCA
TGCCCTTCACTATGGGACAGGGGCTTTTGGCGGCATGCGAGGCATCCCCGATCCACAAAATCCCAATCAAATTCTGCTCT
TTCGTTTAGATCGTCACTGTCAGCGCCTCAGTCAGAGTGCCCGCTTACTTCACTTCGATTTACCGGCTGACAAAATTGAG
CAAATTATTAAAGACTTTGTTCAAAAAAACCGCCCTCAAACCTCCTTCTATATCCGACCATTTGTCTATACCTCCGACCT
AGGAATTGCCCCACGACTCCACAACATCGAAAAGAACTTCTTCGTTTACGGCATCGAACTGGGAGACTATCTCTCCCCCG
ATGGTGTGAGCTGTCGTATCAGTTCCTGGTGCCGACAAGCAGACCACAGCCTACCGTTGCGAGGCAAAATCAGCGGGGCC
TATATCACTTCATCTTTGGCTAAGACAGAAGCTGTAGAATCTGGCTTTGATGAAGCGATTTTGATGAATGCTCAAGGCAA
GGTGAGTGAAGCATCGGGCATGAATATTTTTATTGTCCGCCATGGCAAAATTATTACCCCGGGCTTTGAGCAAGATATTT
TGGAAGGCATCACTCGCGATAGTGTCTTGCAATTAGCGCGTGACTTAGGCATTCCCGTGGTCGAGCGTCCGGTAGACAAG
TCCGAATTACTGATTGCTGATGAAGTGTTTTTAAGCGGAACAGCAGCTAAAATCACACCAGTCAAACGGATCGAGAACTA
CACCACGCCCAGCGAACGACCCATTACTGAAAAGCTCAGAGATAAATTAACAGCCATTACCGAAAATCGCGACTCCCAAT
ATCAGCAATGGGTATTTCCTATTCCGCTCAACTAA

Upstream 100 bases:

>100_bases
GATGTTAGTCTCAATTAGGATGGTGGCAGTTCCATTTCCCAATGGTAGAGGGAAGAATGGATGCTGTCCCACCTAAATTG
TTTGAGCATTGATCTGGGTT

Downstream 100 bases:

>100_bases
GACGTCATAAATTCGATTGAAATAGTCCATCCTGGCTTTAATACCTATTAATTATTGAGATACCTATTAATTTTTAGGTG
CATAATCCCTTGGCGTTGAT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MHNFLPTAYFEHQFVPFKEANISIATHALHYGTGAFGGMRGIPDPQNPNQILLFRLDRHCQRLSQSARLLHFDLPADKIE
QIIKDFVQKNRPQTSFYIRPFVYTSDLGIAPRLHNIEKNFFVYGIELGDYLSPDGVSCRISSWCRQADHSLPLRGKISGA
YITSSLAKTEAVESGFDEAILMNAQGKVSEASGMNIFIVRHGKIITPGFEQDILEGITRDSVLQLARDLGIPVVERPVDK
SELLIADEVFLSGTAAKITPVKRIENYTTPSERPITEKLRDKLTAITENRDSQYQQWVFPIPLN

Sequences:

>Translated_304_residues
MHNFLPTAYFEHQFVPFKEANISIATHALHYGTGAFGGMRGIPDPQNPNQILLFRLDRHCQRLSQSARLLHFDLPADKIE
QIIKDFVQKNRPQTSFYIRPFVYTSDLGIAPRLHNIEKNFFVYGIELGDYLSPDGVSCRISSWCRQADHSLPLRGKISGA
YITSSLAKTEAVESGFDEAILMNAQGKVSEASGMNIFIVRHGKIITPGFEQDILEGITRDSVLQLARDLGIPVVERPVDK
SELLIADEVFLSGTAAKITPVKRIENYTTPSERPITEKLRDKLTAITENRDSQYQQWVFPIPLN
>Mature_304_residues
MHNFLPTAYFEHQFVPFKEANISIATHALHYGTGAFGGMRGIPDPQNPNQILLFRLDRHCQRLSQSARLLHFDLPADKIE
QIIKDFVQKNRPQTSFYIRPFVYTSDLGIAPRLHNIEKNFFVYGIELGDYLSPDGVSCRISSWCRQADHSLPLRGKISGA
YITSSLAKTEAVESGFDEAILMNAQGKVSEASGMNIFIVRHGKIITPGFEQDILEGITRDSVLQLARDLGIPVVERPVDK
SELLIADEVFLSGTAAKITPVKRIENYTTPSERPITEKLRDKLTAITENRDSQYQQWVFPIPLN

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=299, Percent_Identity=33.4448160535117, Blast_Score=183, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6322002, Length=281, Percent_Identity=27.4021352313167, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34272; Mature: 34272

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNFLPTAYFEHQFVPFKEANISIATHALHYGTGAFGGMRGIPDPQNPNQILLFRLDRHC
CCCCCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHH
QRLSQSARLLHFDLPADKIEQIIKDFVQKNRPQTSFYIRPFVYTSDLGIAPRLHNIEKNF
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHCCCCCE
FVYGIELGDYLSPDGVSCRISSWCRQADHSLPLRGKISGAYITSSLAKTEAVESGFDEAI
EEEEEEECCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
LMNAQGKVSEASGMNIFIVRHGKIITPGFEQDILEGITRDSVLQLARDLGIPVVERPVDK
EECCCCCCCCCCCCEEEEEECCEEECCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
SELLIADEVFLSGTAAKITPVKRIENYTTPSERPITEKLRDKLTAITENRDSQYQQWVFP
CCEEEEHHHHCCCCCHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEEE
IPLN
CCCC
>Mature Secondary Structure
MHNFLPTAYFEHQFVPFKEANISIATHALHYGTGAFGGMRGIPDPQNPNQILLFRLDRHC
CCCCCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHH
QRLSQSARLLHFDLPADKIEQIIKDFVQKNRPQTSFYIRPFVYTSDLGIAPRLHNIEKNF
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHCCCCCE
FVYGIELGDYLSPDGVSCRISSWCRQADHSLPLRGKISGAYITSSLAKTEAVESGFDEAI
EEEEEEECCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
LMNAQGKVSEASGMNIFIVRHGKIITPGFEQDILEGITRDSVLQLARDLGIPVVERPVDK
EECCCCCCCCCCCCEEEEEECCEEECCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
SELLIADEVFLSGTAAKITPVKRIENYTTPSERPITEKLRDKLTAITENRDSQYQQWVFP
CCEEEEHHHHCCCCCHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEEE
IPLN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]