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The map label for this gene is wbjE [H]

Identifier: 158336739

GI number: 158336739

Start: 3662118

End: 3663377

Strand: Reverse

Name: wbjE [H]

Synonym: AM1_3607

Alternate gene names: 158336739

Gene position: 3663377-3662118 (Counterclockwise)

Preceding gene: 158336740

Following gene: 158336738

Centisome position: 56.33

GC content: 43.49

Gene sequence:

>1260_bases
ATGAATCGCGTTGTCGTTTTAACAGAATTGTTTTATCCAGAAAGAACATCAACAGCTTATTTTCTAACTGCAATTGCTCA
AGGACTGGTCACAGACTATCCAGTGATAGTCTTTACTGACGCTTCTCTATATGAAAGTCAAGCGTCAAGCTTGGCCTCAT
ATGTTGTTGTAAAAGGGGTGGAAATTCATCGATGTTCTGGAACAGCATTCGATAAGAATTGGCTTCCTGGACGCCTAGTG
AATGGTTGTACCCGTGCGATCGCAATCTTTTTACAAGCCTTATGGCAGTGCCATGCCAGTGATGTGGTCTTAGTCGTCAC
CAATCCACCACTCTTGCCCTTTATGGCATGGTTACTCAAAACGTTGAAGGGGTGTGAGTTTGTTCTCTTGGTGCATGATG
TTTATCCCGAAGTGTTATCTGCTACGGGGCTTGTCCATAACCACTCTCTGGTCTATCGACTAGTGCAAAGGGGCAATCGT
CTGGTTTATGCACAGGCTAGCCGCATCATTACGCTGGGGCGAGATATGCAAAAGCTAGTGGGTCAAAAATTTACTCAGCC
TGTAGAGGGGAAGCTGGTTTGTATTCCCAATTGGGCTGAAACTGAGATGATTTATCCTGTTGATAAGCAGGACAGTACCC
TTTTACAGCGCTTAAAGTTAGTCGATAAGTTTGTAGTGCTCTATGCAGGGAATATGGGCCGGACTCATGACTTAAACATC
TTATTGGATGCAGCAAACATCCTAACGAAAACACATCCTCAGATTCATTTTTTGCTGATTGGGGCCGGGGCTCGGAAGCC
AGAGGTGGAAGCGAGGGTACAAGAGCAAAAATTGCAGAATGTCACGGTGTTACCGTACTTGCCTCATGAGGAAAAAAATG
TGGCCCTTAATTCTTGTGATGTGGGAGTCATCTCATTTGTACCAGGGATGGCAGGAGTGTCAGTACCCAGCCGGATGTAC
AATCAGATGGCTGCGGGCAAACCATTGATTGCGATCGCAGATCATGAATCTGAATTAGCAAAAGTCATTCAAGAAGAAGG
TATTGGTTGGTTAGTTTCACCTCATGATGGTGATCAATTGGTTAAACTCTTAACTCAAATCGTGAATGATTCAGAATTAT
GTAACGACATGGGACACAAAGCAGCGCAAGCTGTTTTATGTAAATATCAGCTCAAACATGCTGTAAGTAAGTATCAATCA
CTTTTTCGAGAGTTAATTCCAAAAACAACCTCTCTAGCCCAAGAAACACTAAAGAAATGA

Upstream 100 bases:

>100_bases
CGATAACGCCAAGATTCGTCAGCAGTCCATCTTTTCGTGGGAGTCAGCTGCAAACAAAACGCTCAGCGTTTATAAAAGCT
TACTCATTTAAGGCCTGAGT

Downstream 100 bases:

>100_bases
AATAGGCGCTTACTCCAACTTAACTGAAAGCTCATAGCAGCATGGCCATATCACAGTTATAAGAGAAACGCTGGAGCTGT
TCTGTTTGGGCAGAACAATA

Product: glycosyl transferase, group 1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNRVVVLTELFYPERTSTAYFLTAIAQGLVTDYPVIVFTDASLYESQASSLASYVVVKGVEIHRCSGTAFDKNWLPGRLV
NGCTRAIAIFLQALWQCHASDVVLVVTNPPLLPFMAWLLKTLKGCEFVLLVHDVYPEVLSATGLVHNHSLVYRLVQRGNR
LVYAQASRIITLGRDMQKLVGQKFTQPVEGKLVCIPNWAETEMIYPVDKQDSTLLQRLKLVDKFVVLYAGNMGRTHDLNI
LLDAANILTKTHPQIHFLLIGAGARKPEVEARVQEQKLQNVTVLPYLPHEEKNVALNSCDVGVISFVPGMAGVSVPSRMY
NQMAAGKPLIAIADHESELAKVIQEEGIGWLVSPHDGDQLVKLLTQIVNDSELCNDMGHKAAQAVLCKYQLKHAVSKYQS
LFRELIPKTTSLAQETLKK

Sequences:

>Translated_419_residues
MNRVVVLTELFYPERTSTAYFLTAIAQGLVTDYPVIVFTDASLYESQASSLASYVVVKGVEIHRCSGTAFDKNWLPGRLV
NGCTRAIAIFLQALWQCHASDVVLVVTNPPLLPFMAWLLKTLKGCEFVLLVHDVYPEVLSATGLVHNHSLVYRLVQRGNR
LVYAQASRIITLGRDMQKLVGQKFTQPVEGKLVCIPNWAETEMIYPVDKQDSTLLQRLKLVDKFVVLYAGNMGRTHDLNI
LLDAANILTKTHPQIHFLLIGAGARKPEVEARVQEQKLQNVTVLPYLPHEEKNVALNSCDVGVISFVPGMAGVSVPSRMY
NQMAAGKPLIAIADHESELAKVIQEEGIGWLVSPHDGDQLVKLLTQIVNDSELCNDMGHKAAQAVLCKYQLKHAVSKYQS
LFRELIPKTTSLAQETLKK
>Mature_419_residues
MNRVVVLTELFYPERTSTAYFLTAIAQGLVTDYPVIVFTDASLYESQASSLASYVVVKGVEIHRCSGTAFDKNWLPGRLV
NGCTRAIAIFLQALWQCHASDVVLVVTNPPLLPFMAWLLKTLKGCEFVLLVHDVYPEVLSATGLVHNHSLVYRLVQRGNR
LVYAQASRIITLGRDMQKLVGQKFTQPVEGKLVCIPNWAETEMIYPVDKQDSTLLQRLKLVDKFVVLYAGNMGRTHDLNI
LLDAANILTKTHPQIHFLLIGAGARKPEVEARVQEQKLQNVTVLPYLPHEEKNVALNSCDVGVISFVPGMAGVSVPSRMY
NQMAAGKPLIAIADHESELAKVIQEEGIGWLVSPHDGDQLVKLLTQIVNDSELCNDMGHKAAQAVLCKYQLKHAVSKYQS
LFRELIPKTTSLAQETLKK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788363, Length=178, Percent_Identity=28.0898876404494, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 46554; Mature: 46554

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVVVLTELFYPERTSTAYFLTAIAQGLVTDYPVIVFTDASLYESQASSLASYVVVKGV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCC
EIHRCSGTAFDKNWLPGRLVNGCTRAIAIFLQALWQCHASDVVLVVTNPPLLPFMAWLLK
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
TLKGCEFVLLVHDVYPEVLSATGLVHNHSLVYRLVQRGNRLVYAQASRIITLGRDMQKLV
HHCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHHH
GQKFTQPVEGKLVCIPNWAETEMIYPVDKQDSTLLQRLKLVDKFVVLYAGNMGRTHDLNI
HHHCCCCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCEE
LLDAANILTKTHPQIHFLLIGAGARKPEVEARVQEQKLQNVTVLPYLPHEEKNVALNSCD
EEEHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCC
VGVISFVPGMAGVSVPSRMYNQMAAGKPLIAIADHESELAKVIQEEGIGWLVSPHDGDQL
CHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCEEEECCCCHHHH
VKLLTQIVNDSELCNDMGHKAAQAVLCKYQLKHAVSKYQSLFRELIPKTTSLAQETLKK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MNRVVVLTELFYPERTSTAYFLTAIAQGLVTDYPVIVFTDASLYESQASSLASYVVVKGV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCC
EIHRCSGTAFDKNWLPGRLVNGCTRAIAIFLQALWQCHASDVVLVVTNPPLLPFMAWLLK
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
TLKGCEFVLLVHDVYPEVLSATGLVHNHSLVYRLVQRGNRLVYAQASRIITLGRDMQKLV
HHCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHHH
GQKFTQPVEGKLVCIPNWAETEMIYPVDKQDSTLLQRLKLVDKFVVLYAGNMGRTHDLNI
HHHCCCCCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCEE
LLDAANILTKTHPQIHFLLIGAGARKPEVEARVQEQKLQNVTVLPYLPHEEKNVALNSCD
EEEHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCC
VGVISFVPGMAGVSVPSRMYNQMAAGKPLIAIADHESELAKVIQEEGIGWLVSPHDGDQL
CHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCEEEECCCCHHHH
VKLLTQIVNDSELCNDMGHKAAQAVLCKYQLKHAVSKYQSLFRELIPKTTSLAQETLKK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400585; 10828401; 12057956 [H]