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The map label for this gene is thiG

Identifier: 158336731

GI number: 158336731

Start: 3654795

End: 3655622

Strand: Reverse

Name: thiG

Synonym: AM1_3599

Alternate gene names: 158336731

Gene position: 3655622-3654795 (Counterclockwise)

Preceding gene: 158336734

Following gene: 158336730

Centisome position: 56.21

GC content: 52.17

Gene sequence:

>828_bases
ATGCAAACCATTGATTCTTCCTCTTCGGCTGCGACGACTGAGACCCTACAAATCGGTCATCGACAGTTCCAGTCAAGGTT
AATGACCGGTACCGGCAAATATGACGATTTTGAAACGATGCGGCGCAGTATTGCGGCCAGTGGTTGTGAAATTGTGACTG
TCGCTGTGCGTCGGGTGCAAACCAACGCCCCTGGACATCAAGGCTTAGCTGAAGCTGTGGATTGGAACAAGGTATGGATG
CTCCCGAATACCGCTGGTTGCCAAACAGCAGAAGAGGCCGTTCGGGTCGCCCGGCTCGGTCGAGAAATGGCCAAGCTGTT
AGGGCAAGAAGACAATAACTTTATCAAGCTAGAGGTGATCCCAGACTCTAAGTACTTGCTACCTGATCCCATCGGCACCT
TGGAGGCGGCTGAACAATTGGTTAAAGAGGGGTTCGCTGTTCTGCCTTATATTAATGCCGATCCCCTGTTAGCTAAGCGC
CTAGAAGAAGTCGGCTGTGCTACCGTCATGCCCCTTGGATCGCCTATTGGATCGGGGCAAGGGATCAAAAATATGGCCAA
TATTCAGATCATTATTGAGAATGCCACAGTACCAGTCGTCGTAGACGCCGGCATCGGTTCTCCCAGTGAAGCAGCACAAG
CCATGGAGATGGGGGCTGATGCAGTGTTAATCAATACAGCCATTGCTAAAGCCCAAGATCCAGTGATGATGGCAACTGCC
ATGGGGATGGCCACCCAAGCAGGACGATTCGCCTACCAATCGGGACGCATCCCAGTCAAAGCCTATGCCAGTGCCAGTTC
TCCCTTGGCGGGTCGAATTTCTAGCTAG

Upstream 100 bases:

>100_bases
CCATGACCGCAAGTTGGTTTCTTATAATGTGAGATACTTCACTAGCCTAGCGGCAGTTTCCCTTCATTGCTGAACTTTTC
TTAGACCTTTATCCTTGGCT

Downstream 100 bases:

>100_bases
GGAAGAAGTTTCTCTTTTCCGCGACTAGCTTATTAAAAAAATCTAAAGGCTCTTCTCACAACCTGAGGATAAAAAAAGAG
ATTATGACGTTCTCGTTACA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MQTIDSSSSAATTETLQIGHRQFQSRLMTGTGKYDDFETMRRSIAASGCEIVTVAVRRVQTNAPGHQGLAEAVDWNKVWM
LPNTAGCQTAEEAVRVARLGREMAKLLGQEDNNFIKLEVIPDSKYLLPDPIGTLEAAEQLVKEGFAVLPYINADPLLAKR
LEEVGCATVMPLGSPIGSGQGIKNMANIQIIIENATVPVVVDAGIGSPSEAAQAMEMGADAVLINTAIAKAQDPVMMATA
MGMATQAGRFAYQSGRIPVKAYASASSPLAGRISS

Sequences:

>Translated_275_residues
MQTIDSSSSAATTETLQIGHRQFQSRLMTGTGKYDDFETMRRSIAASGCEIVTVAVRRVQTNAPGHQGLAEAVDWNKVWM
LPNTAGCQTAEEAVRVARLGREMAKLLGQEDNNFIKLEVIPDSKYLLPDPIGTLEAAEQLVKEGFAVLPYINADPLLAKR
LEEVGCATVMPLGSPIGSGQGIKNMANIQIIIENATVPVVVDAGIGSPSEAAQAMEMGADAVLINTAIAKAQDPVMMATA
MGMATQAGRFAYQSGRIPVKAYASASSPLAGRISS
>Mature_275_residues
MQTIDSSSSAATTETLQIGHRQFQSRLMTGTGKYDDFETMRRSIAASGCEIVTVAVRRVQTNAPGHQGLAEAVDWNKVWM
LPNTAGCQTAEEAVRVARLGREMAKLLGQEDNNFIKLEVIPDSKYLLPDPIGTLEAAEQLVKEGFAVLPYINADPLLAKR
LEEVGCATVMPLGSPIGSGQGIKNMANIQIIIENATVPVVVDAGIGSPSEAAQAMEMGADAVLINTAIAKAQDPVMMATA
MGMATQAGRFAYQSGRIPVKAYASASSPLAGRISS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:
GO:0003824: Thiazole synthase
GO:0005737: Thiazole synthase
GO:0008152: Thiazole synthase
GO:0009228: Thiazole synthase
GO:0016829: Thiazole synthase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=260, Percent_Identity=54.6153846153846, Blast_Score=264, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_ACAM1 (B0C390)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517905.1
- ProteinModelPortal:   B0C390
- SMR:   B0C390
- GeneID:   5682405
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3599
- HOGENOM:   HBG296821
- OMA:   PIIIDAG
- ProtClustDB:   PRK00208
- BioCyc:   AMAR329726:AM1_3599-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 29081; Mature: 29081

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: ACT_SITE 116-116 BINDING 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTIDSSSSAATTETLQIGHRQFQSRLMTGTGKYDDFETMRRSIAASGCEIVTVAVRRVQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
TNAPGHQGLAEAVDWNKVWMLPNTAGCQTAEEAVRVARLGREMAKLLGQEDNNFIKLEVI
CCCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
PDSKYLLPDPIGTLEAAEQLVKEGFAVLPYINADPLLAKRLEEVGCATVMPLGSPIGSGQ
CCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCC
GIKNMANIQIIIENATVPVVVDAGIGSPSEAAQAMEMGADAVLINTAIAKAQDPVMMATA
CCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCEEEEHHHHHHCCCCCHHHHH
MGMATQAGRFAYQSGRIPVKAYASASSPLAGRISS
HHHHHHCCHHHHHCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQTIDSSSSAATTETLQIGHRQFQSRLMTGTGKYDDFETMRRSIAASGCEIVTVAVRRVQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
TNAPGHQGLAEAVDWNKVWMLPNTAGCQTAEEAVRVARLGREMAKLLGQEDNNFIKLEVI
CCCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
PDSKYLLPDPIGTLEAAEQLVKEGFAVLPYINADPLLAKRLEEVGCATVMPLGSPIGSGQ
CCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCC
GIKNMANIQIIIENATVPVVVDAGIGSPSEAAQAMEMGADAVLINTAIAKAQDPVMMATA
CCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCEEEEHHHHHHCCCCCHHHHH
MGMATQAGRFAYQSGRIPVKAYASASSPLAGRISS
HHHHHHCCHHHHHCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA