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The map label for this gene is gldA [C]

Identifier: 158336708

GI number: 158336708

Start: 3624276

End: 3625445

Strand: Direct

Name: gldA [C]

Synonym: AM1_3576

Alternate gene names: 158336708

Gene position: 3624276-3625445 (Clockwise)

Preceding gene: 158336707

Following gene: 158336709

Centisome position: 55.73

GC content: 52.74

Gene sequence:

>1170_bases
ATGTCTAAGTTCTCATCACCCTCTGTCTCCTCCTCGGTTGAGTGCGCCAGTCAAGCCATTGCACCCCAATGTGTCGTTCG
AGCTGAGCAAAGTCTGGCTCAATTAGAATCTCTGATCCAGCCCTATGGCCAACGACCGCTAGTGGTCGCTGGAGAGCATA
GCTTAGCAGTCTTGCAGCCTTACTTGGCTGCAATTTCAACTTTAACTTTGGAGCTGGCGGTTGCCCATTATGGTCAAGAT
TGCAGTGAAGCCAGTCTCAAGGCTTTAGGAGAGGCGATGACCTGTCATCGTGCAGACGTCATTGTTGGGGTGGGCGGTGG
AAAAGCGTTAGATGCAGCCAAACTCTTGGCCCATCAAAACCGCTGTCCCGTGATTACGATTCCCACCTCTGCCGCGACCT
GTGCGGCCTGGACGGCGTTATCGAATTTATATTCGGAGACAGGAGCATTTCAGTATGATGTCGGCTTACCTCGATGTCCT
GATTTACTAATCCTGGATTATGAGGTGATCCAAACTGCTCCCCAGCGAACGCTGATTGCTGGCATTGGGGATGCGTTAGC
GAAGTGGTACGAAGCGTCTGTGAGTAGCGGCCATTCCCAGGAAACCCTAACGATTGCTGCTGTGCAACAGGCCCGCGTGC
TCAGAGATATTCTGTTTCAGAAATCCATGAAGGCCATTACCCAACCGGGCAGTGATGAGTGGCGACAAGTAGTGGATGCC
ACGGTGTTAATGGCAGGGGTTGTGGGAGGATTGGGTGGAGCTCAATGCCGTACTGTGGCTGCCCATGCCGTCCATAATGG
GCTGACCCATCTCTCGCCGACCCGCGCCAGCTTACATGGGGAAAAAGTGGCCTATGGTATTTTGGTGCAATTGCGTTTAG
AAGAACTGGTCCAGAATAACCACCTCGCGACAACGGCTCGCCATCAGCTGCTCAAATTCTATAAAGAGATTGGCTTACCG
ATGACCCTGGCCGATTTAGGGATGGAGGATGTGTCTCTGAAGAACTTACAAATGGCGGCGCAAGTGGCTTGCCAGCCAGA
TTCAGATCTCCACCATTTACCCTTTACGGTGATGCCAGATCAGCTGCTAGCGGCGATGGTCTCTACGGCTAGTCTCATGG
CGGACGGTAGTGCTAATCCAAGCTCTCCATCGGAAGAGGTTTGCCCATGA

Upstream 100 bases:

>100_bases
TTGATCCCTCCTCTAGTTAAAATCAATATTGGAAGTCAGCTTCCCTAGCCAAATGGATATCAGGAGAGGACTGACTTAAT
TGCTCCGTACCGTCCCACCT

Downstream 100 bases:

>100_bases
GTTTGGATTGGATCAAACCGGCTGACCGGTTGAGTGCATTGCCACCCTATGTATTTGCCCGTTTAGATGAACTCAAAACC
TCGGCTCGTCAGCAAGGGCT

Product: glycerol dehydrogenase

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSKFSSPSVSSSVECASQAIAPQCVVRAEQSLAQLESLIQPYGQRPLVVAGEHSLAVLQPYLAAISTLTLELAVAHYGQD
CSEASLKALGEAMTCHRADVIVGVGGGKALDAAKLLAHQNRCPVITIPTSAATCAAWTALSNLYSETGAFQYDVGLPRCP
DLLILDYEVIQTAPQRTLIAGIGDALAKWYEASVSSGHSQETLTIAAVQQARVLRDILFQKSMKAITQPGSDEWRQVVDA
TVLMAGVVGGLGGAQCRTVAAHAVHNGLTHLSPTRASLHGEKVAYGILVQLRLEELVQNNHLATTARHQLLKFYKEIGLP
MTLADLGMEDVSLKNLQMAAQVACQPDSDLHHLPFTVMPDQLLAAMVSTASLMADGSANPSSPSEEVCP

Sequences:

>Translated_389_residues
MSKFSSPSVSSSVECASQAIAPQCVVRAEQSLAQLESLIQPYGQRPLVVAGEHSLAVLQPYLAAISTLTLELAVAHYGQD
CSEASLKALGEAMTCHRADVIVGVGGGKALDAAKLLAHQNRCPVITIPTSAATCAAWTALSNLYSETGAFQYDVGLPRCP
DLLILDYEVIQTAPQRTLIAGIGDALAKWYEASVSSGHSQETLTIAAVQQARVLRDILFQKSMKAITQPGSDEWRQVVDA
TVLMAGVVGGLGGAQCRTVAAHAVHNGLTHLSPTRASLHGEKVAYGILVQLRLEELVQNNHLATTARHQLLKFYKEIGLP
MTLADLGMEDVSLKNLQMAAQVACQPDSDLHHLPFTVMPDQLLAAMVSTASLMADGSANPSSPSEEVCP
>Mature_388_residues
SKFSSPSVSSSVECASQAIAPQCVVRAEQSLAQLESLIQPYGQRPLVVAGEHSLAVLQPYLAAISTLTLELAVAHYGQDC
SEASLKALGEAMTCHRADVIVGVGGGKALDAAKLLAHQNRCPVITIPTSAATCAAWTALSNLYSETGAFQYDVGLPRCPD
LLILDYEVIQTAPQRTLIAGIGDALAKWYEASVSSGHSQETLTIAAVQQARVLRDILFQKSMKAITQPGSDEWRQVVDAT
VLMAGVVGGLGGAQCRTVAAHAVHNGLTHLSPTRASLHGEKVAYGILVQLRLEELVQNNHLATTARHQLLKFYKEIGLPM
TLADLGMEDVSLKNLQMAAQVACQPDSDLHHLPFTVMPDQLLAAMVSTASLMADGSANPSSPSEEVCP

Specific function: Unknown

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:
GO:0016491: Glycerol dehydrogenase, putative
GO:0016614: Glycerol dehydrogenase, putative
GO:0046872: Glycerol dehydrogenase, putative
GO:0055114: Glycerol dehydrogenase, putative

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1786815, Length=302, Percent_Identity=41.0596026490066, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87082352, Length=359, Percent_Identity=33.7047353760446, Blast_Score=168, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 41211; Mature: 41079

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFSSPSVSSSVECASQAIAPQCVVRAEQSLAQLESLIQPYGQRPLVVAGEHSLAVLQP
CCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHH
YLAAISTLTLELAVAHYGQDCSEASLKALGEAMTCHRADVIVGVGGGKALDAAKLLAHQN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCC
RCPVITIPTSAATCAAWTALSNLYSETGAFQYDVGLPRCPDLLILDYEVIQTAPQRTLIA
CCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECHHHHHCCCCHHHHH
GIGDALAKWYEASVSSGHSQETLTIAAVQQARVLRDILFQKSMKAITQPGSDEWRQVVDA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TVLMAGVVGGLGGAQCRTVAAHAVHNGLTHLSPTRASLHGEKVAYGILVQLRLEELVQNN
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCC
HLATTARHQLLKFYKEIGLPMTLADLGMEDVSLKNLQMAAQVACQPDSDLHHLPFTVMPD
CCHHHHHHHHHHHHHHHCCCEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECHH
QLLAAMVSTASLMADGSANPSSPSEEVCP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKFSSPSVSSSVECASQAIAPQCVVRAEQSLAQLESLIQPYGQRPLVVAGEHSLAVLQP
CCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHH
YLAAISTLTLELAVAHYGQDCSEASLKALGEAMTCHRADVIVGVGGGKALDAAKLLAHQN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCC
RCPVITIPTSAATCAAWTALSNLYSETGAFQYDVGLPRCPDLLILDYEVIQTAPQRTLIA
CCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECHHHHHCCCCHHHHH
GIGDALAKWYEASVSSGHSQETLTIAAVQQARVLRDILFQKSMKAITQPGSDEWRQVVDA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TVLMAGVVGGLGGAQCRTVAAHAVHNGLTHLSPTRASLHGEKVAYGILVQLRLEELVQNN
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCC
HLATTARHQLLKFYKEIGLPMTLADLGMEDVSLKNLQMAAQVACQPDSDLHHLPFTVMPD
CCHHHHHHHHHHHHHHHCCCEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECHH
QLLAAMVSTASLMADGSANPSSPSEEVCP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]