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The map label for this gene is amtB [C]

Identifier: 158336665

GI number: 158336665

Start: 3572503

End: 3573966

Strand: Reverse

Name: amtB [C]

Synonym: AM1_3533

Alternate gene names: 158336665

Gene position: 3573966-3572503 (Counterclockwise)

Preceding gene: 158336666

Following gene: 158336663

Centisome position: 54.95

GC content: 50.55

Gene sequence:

>1464_bases
GTGGTGATGTCTAAGCCAATTAAATCGAGGTCTAAAAACCTCAAGCGCAGAAAGCGCTTATCTCCTGCTTGGCAGCTTTG
CATGCCAATGACAGCAATAGTTTTATTATTTTGGTCTGTGGCCGCTGTTGCCCAAGAAGCAGCTCCCCCTGACCCCACAG
CAACCCTCACCGTTGGGTTAGATACGGTTTGGGTACTGCTCTGTGCTTTTTTAGTCTTTTTTATGAACGCCGGATTCGGC
ATGCTGGAAACGGGCTTCTGTCGTCAAAAGAATGCCGTCAATATCCTAGCCAAAAACTTGATTGTGTTTGCATTAGCCAC
CATTGCCTACTGGGCTATTGGTTTTGGCTTGATGTTTGGCGATGGCAATCCGGTCTTAGGGACTGCCGGGTGGTTCGTCG
GTGGTGCTGACAACAGTCCAGCCACTGGAGATGCCTATGAAGGTATTTTTGGAGCTTTGAATTGGACAGGAGTTCCACTC
TTTGCCAAATTCCTCTTCCAACTTGTGTTTGCTGGAACTGCAGCCACCATTGTGTCTGGAGCAGTGGCTGAGCGCATTAA
GTTTGTCTCTTTCTTGATTTTTAGCTTAGTGCTCACTGGCATTCTCTATCCCATTTCTGGTCATTGGATTTGGGGTGGCG
GCTGGTTAGCAGGTATGGGCTTTTGGGACTTTGCCGGTTCTACAGTCGTCCACTCTGTCGGAGGCTGGGCAGCTTTCGTC
GGTGCTGCGTTCCTTGGACCTCGGTTAGGTCGCTATTCTCAAACTGGGGCCACTGCTATGCCTGGTCACAATATGAGTAT
TGCTACTCTAGGGTGTTTGATTCTGTGGTTAGGATGGTTTGGCTTTAACCCAGGATCCACTATGGGGGTAGGGGATGGTG
CTGCCATTGCTCATGTGGCCCTGACTACGAATATTGCGGCTGCTTTCGGTGGCGTTGCGGCAACTTTCACGGCTTGGTTC
TACCTCGGCAAGCCTGATTTATCCATGGTCATCAATGGCATCTTGGCTGGCTTAGTGGGCATTACGGCCCCTTGTGCTTG
GGTGACGGTTCCTTGGGCTGCAGTGATTGGTCTGATTGCTGGGGTGATTGTTGTGTTTGCGGTCACTATCATCGATGGCA
TGAAGATTGATGACCCAGTGGGTGCCATCTCTGTTCACTGTGTCTGTGGTGTTTGGGGCACTTTGGCGGTCGGTCTATTT
TCTCAAGGACCAGATGGTCTGATCTATGGCGAAGGAGGCGGTCCTGCTAAGGGTCTTATCCTAGGCGGTGGCTTTGAACA
AATGATTCCGCAAATCGTTGGTATCCTGGCTGTCGGTGCTTTCACGTTGGTCACCACGGCCATTGCTTGGGCTGCCATCA
AGGCAATTGCTGGACTCCGGGTTCCTGAAGAAGAAGAAATCAAAGGACTCGATATCAGCGAACATGGCATGGAAGCCTAT
AGCGGTTTCCTTAAAGAGGCATAA

Upstream 100 bases:

>100_bases
AGATGGCCCTCAATTTGTTATTTAGTCATGGTTTCGATGCCTTTATCCAAATCAATTACTAGAGCAATTGGTTGTCCATC
GTTTATCTAGGAAACGTATT

Downstream 100 bases:

>100_bases
GGCTTCCTTGTACCAGCCCCATCCACTGGGTTGAATATGTCATCTTTCATCAACAATAGCCCCCAGAACTACACCAGTGC
TGGGGGCTATTGTTTTTTGT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MVMSKPIKSRSKNLKRRKRLSPAWQLCMPMTAIVLLFWSVAAVAQEAAPPDPTATLTVGLDTVWVLLCAFLVFFMNAGFG
MLETGFCRQKNAVNILAKNLIVFALATIAYWAIGFGLMFGDGNPVLGTAGWFVGGADNSPATGDAYEGIFGALNWTGVPL
FAKFLFQLVFAGTAATIVSGAVAERIKFVSFLIFSLVLTGILYPISGHWIWGGGWLAGMGFWDFAGSTVVHSVGGWAAFV
GAAFLGPRLGRYSQTGATAMPGHNMSIATLGCLILWLGWFGFNPGSTMGVGDGAAIAHVALTTNIAAAFGGVAATFTAWF
YLGKPDLSMVINGILAGLVGITAPCAWVTVPWAAVIGLIAGVIVVFAVTIIDGMKIDDPVGAISVHCVCGVWGTLAVGLF
SQGPDGLIYGEGGGPAKGLILGGGFEQMIPQIVGILAVGAFTLVTTAIAWAAIKAIAGLRVPEEEEIKGLDISEHGMEAY
SGFLKEA

Sequences:

>Translated_487_residues
MVMSKPIKSRSKNLKRRKRLSPAWQLCMPMTAIVLLFWSVAAVAQEAAPPDPTATLTVGLDTVWVLLCAFLVFFMNAGFG
MLETGFCRQKNAVNILAKNLIVFALATIAYWAIGFGLMFGDGNPVLGTAGWFVGGADNSPATGDAYEGIFGALNWTGVPL
FAKFLFQLVFAGTAATIVSGAVAERIKFVSFLIFSLVLTGILYPISGHWIWGGGWLAGMGFWDFAGSTVVHSVGGWAAFV
GAAFLGPRLGRYSQTGATAMPGHNMSIATLGCLILWLGWFGFNPGSTMGVGDGAAIAHVALTTNIAAAFGGVAATFTAWF
YLGKPDLSMVINGILAGLVGITAPCAWVTVPWAAVIGLIAGVIVVFAVTIIDGMKIDDPVGAISVHCVCGVWGTLAVGLF
SQGPDGLIYGEGGGPAKGLILGGGFEQMIPQIVGILAVGAFTLVTTAIAWAAIKAIAGLRVPEEEEIKGLDISEHGMEAY
SGFLKEA
>Mature_487_residues
MVMSKPIKSRSKNLKRRKRLSPAWQLCMPMTAIVLLFWSVAAVAQEAAPPDPTATLTVGLDTVWVLLCAFLVFFMNAGFG
MLETGFCRQKNAVNILAKNLIVFALATIAYWAIGFGLMFGDGNPVLGTAGWFVGGADNSPATGDAYEGIFGALNWTGVPL
FAKFLFQLVFAGTAATIVSGAVAERIKFVSFLIFSLVLTGILYPISGHWIWGGGWLAGMGFWDFAGSTVVHSVGGWAAFV
GAAFLGPRLGRYSQTGATAMPGHNMSIATLGCLILWLGWFGFNPGSTMGVGDGAAIAHVALTTNIAAAFGGVAATFTAWF
YLGKPDLSMVINGILAGLVGITAPCAWVTVPWAAVIGLIAGVIVVFAVTIIDGMKIDDPVGAISVHCVCGVWGTLAVGLF
SQGPDGLIYGEGGGPAKGLILGGGFEQMIPQIVGILAVGAFTLVTTAIAWAAIKAIAGLRVPEEEEIKGLDISEHGMEAY
SGFLKEA

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=424, Percent_Identity=35.1415094339623, Blast_Score=207, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI32564042, Length=429, Percent_Identity=35.6643356643357, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=37.8684807256236, Blast_Score=224, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17550180, Length=440, Percent_Identity=35.2272727272727, Blast_Score=218, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI133931100, Length=448, Percent_Identity=32.8125, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321559, Length=441, Percent_Identity=29.4784580498866, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325396, Length=423, Percent_Identity=30.9692671394799, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324187, Length=451, Percent_Identity=28.159645232816, Blast_Score=143, Evalue=8e-35,
Organism=Drosophila melanogaster, GI161078314, Length=425, Percent_Identity=40.7058823529412, Blast_Score=249, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50741; Mature: 50741

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMSKPIKSRSKNLKRRKRLSPAWQLCMPMTAIVLLFWSVAAVAQEAAPPDPTATLTVGL
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECH
DTVWVLLCAFLVFFMNAGFGMLETGFCRQKNAVNILAKNLIVFALATIAYWAIGFGLMFG
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
DGNPVLGTAGWFVGGADNSPATGDAYEGIFGALNWTGVPLFAKFLFQLVFAGTAATIVSG
CCCCCEECCCCEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVAERIKFVSFLIFSLVLTGILYPISGHWIWGGGWLAGMGFWDFAGSTVVHSVGGWAAFV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHCCCHHHHCCCHHHHHHCHHHHHH
GAAFLGPRLGRYSQTGATAMPGHNMSIATLGCLILWLGWFGFNPGSTMGVGDGAAIAHVA
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
LTTNIAAAFGGVAATFTAWFYLGKPDLSMVINGILAGLVGITAPCAWVTVPWAAVIGLIA
HHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GVIVVFAVTIIDGMKIDDPVGAISVHCVCGVWGTLAVGLFSQGPDGLIYGEGGGPAKGLI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEE
LGGGFEQMIPQIVGILAVGAFTLVTTAIAWAAIKAIAGLRVPEEEEIKGLDISEHGMEAY
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHH
SGFLKEA
HHHHHCC
>Mature Secondary Structure
MVMSKPIKSRSKNLKRRKRLSPAWQLCMPMTAIVLLFWSVAAVAQEAAPPDPTATLTVGL
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECH
DTVWVLLCAFLVFFMNAGFGMLETGFCRQKNAVNILAKNLIVFALATIAYWAIGFGLMFG
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
DGNPVLGTAGWFVGGADNSPATGDAYEGIFGALNWTGVPLFAKFLFQLVFAGTAATIVSG
CCCCCEECCCCEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVAERIKFVSFLIFSLVLTGILYPISGHWIWGGGWLAGMGFWDFAGSTVVHSVGGWAAFV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHCCCHHHHCCCHHHHHHCHHHHHH
GAAFLGPRLGRYSQTGATAMPGHNMSIATLGCLILWLGWFGFNPGSTMGVGDGAAIAHVA
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
LTTNIAAAFGGVAATFTAWFYLGKPDLSMVINGILAGLVGITAPCAWVTVPWAAVIGLIA
HHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GVIVVFAVTIIDGMKIDDPVGAISVHCVCGVWGTLAVGLFSQGPDGLIYGEGGGPAKGLI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEE
LGGGFEQMIPQIVGILAVGAFTLVTTAIAWAAIKAIAGLRVPEEEEIKGLDISEHGMEAY
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHH
SGFLKEA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]