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The map label for this gene is gatA

Identifier: 158336663

GI number: 158336663

Start: 3568642

End: 3570090

Strand: Reverse

Name: gatA

Synonym: AM1_3531

Alternate gene names: 158336663

Gene position: 3570090-3568642 (Counterclockwise)

Preceding gene: 158336665

Following gene: 158336661

Centisome position: 54.89

GC content: 49.62

Gene sequence:

>1449_bases
ATGGCATCCATCCAAGCGTTGCATCAAGAGCTGATCACTAAAGAGCGCTCAGCCCAAGAAATTACCGAAGCAGCCCTGGA
AACCATTCATCAATTAGAACCCAAACTCCATAGTTTTTTGGCCATTACTGCTGACCAAGCACTCGCCCAAGCTAAACAGG
TCGATGCTCAGTTAGCTGCGGGGGAAGAGATTGGTTTACTAGCGGGGATTCCCATTGGTATCAAAGATAATATGTGTACC
AAAGGAATTGCTACAACCTGTGGGTCAAAAATCCTTCAAAACTTTATTCCCCCTTACGAGTCTACCGTAACCCAAAAATT
AGCGGCAGCGGGGGCAGTTATGGTGGGTAAAACCAACCTAGATGAATTTGCCATGGGCAGCTCCACAGAAAACTCTGCCT
ATCAAGTCACGGGCAATCCCTGGGATGTCTCCCGCGTGCCGGGCGGTTCCTCGGGGGGCTCGGCTGCAGCGGTAGCGGCT
GATGAATGTGTGGTATCCATTGGCTCGGATACAGGTGGCTCTATTCGGCAGCCAGCAGCCCTCTGTGGTGTGGTTGGTCT
CAAGCCTACCTACGGATTAGTATCTCGCTTTGGTCTGGTGGCTTATGCCTCTTCCTTGGATCAAATTGGTCCTTTTGGTC
GCACCGTCGAAGATGCAGCCATCCTATTACAAGAAATCGCAGGTTACGACCCCCAAGACTCTACTAGCCTCAAAGTCGAA
ATTCCTGATTATTCCAAGTCTCTGATTCCTGACCTTAAAGGGAAAAAGGTAGGAGTGATCACCGAAACCTTTGGGGAAGG
TTTAGATGAGGTCGTAGAAAAGGTCGTTCGCAAGGCGATCGATCAATTAGCTGAACTAGGCGCTGAGGTCCAAGAGATTT
CTTGTCCACGCTTCCGCTATGGGTTGCCCACGTATTATGTGATTGCCCCGTCGGAAGCCTCTGCCAACCTAGCCCGCTAC
GATGGCGTCAAGTATGGCTACCGCACCGATGACCCCGACGATCTGATGTCTATGTACACGAATACTCGGGCTGAGGGGTT
CGGGGCTGAAGTGAAACGCCGAATTATGATTGGCACCTATGCCCTGTCTGCGGGCTATTACGATGCTTACTATCTAAAAG
CCCAAAAAGTCCGCACGTTAATCAAAGAAGACTTTGAGAAGGCGTTTGAGAGTGTAGATGTGTTGGTCTGTCCCACCACG
CCAACAACCGCATTTAAGGCCGGGGAGAAAACAGCTGATCCCTTAAGCATGTATCTCTCTGACTTAATGACGATTCCTGT
GAATTTAGCTGGATTACCGGGTTTGAGCCTACCCTGTGGCTTTGATGATCAAGGATTACCGATTGGTTTGCAGATGATTG
GTAATGTCTTACGGGAAGATCAGGTGCTACAAGCGGCCTATGCCTATGAGCAATCTACAGAATGGCACAAGGCATCTCCC
AAACTATAA

Upstream 100 bases:

>100_bases
GAGGCTGATGCTGATGCCAAATAAAGGCGATATAAAGCGGGTGAGTCATAGGTCACCGTATACTGAGGATTGGCACATTT
TGAACAGAAGGATTTGCTGC

Downstream 100 bases:

>100_bases
TCTCATTCGTACATTTGCCCGCAGTCAAGAAACACTTGAAATTGTTGCCAAGTAACCCTTTCTAAATCGTTCTGAGGAGA
AGGCATATCGTCTGAATTTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MASIQALHQELITKERSAQEITEAALETIHQLEPKLHSFLAITADQALAQAKQVDAQLAAGEEIGLLAGIPIGIKDNMCT
KGIATTCGSKILQNFIPPYESTVTQKLAAAGAVMVGKTNLDEFAMGSSTENSAYQVTGNPWDVSRVPGGSSGGSAAAVAA
DECVVSIGSDTGGSIRQPAALCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQEIAGYDPQDSTSLKVE
IPDYSKSLIPDLKGKKVGVITETFGEGLDEVVEKVVRKAIDQLAELGAEVQEISCPRFRYGLPTYYVIAPSEASANLARY
DGVKYGYRTDDPDDLMSMYTNTRAEGFGAEVKRRIMIGTYALSAGYYDAYYLKAQKVRTLIKEDFEKAFESVDVLVCPTT
PTTAFKAGEKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDDQGLPIGLQMIGNVLREDQVLQAAYAYEQSTEWHKASP
KL

Sequences:

>Translated_482_residues
MASIQALHQELITKERSAQEITEAALETIHQLEPKLHSFLAITADQALAQAKQVDAQLAAGEEIGLLAGIPIGIKDNMCT
KGIATTCGSKILQNFIPPYESTVTQKLAAAGAVMVGKTNLDEFAMGSSTENSAYQVTGNPWDVSRVPGGSSGGSAAAVAA
DECVVSIGSDTGGSIRQPAALCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQEIAGYDPQDSTSLKVE
IPDYSKSLIPDLKGKKVGVITETFGEGLDEVVEKVVRKAIDQLAELGAEVQEISCPRFRYGLPTYYVIAPSEASANLARY
DGVKYGYRTDDPDDLMSMYTNTRAEGFGAEVKRRIMIGTYALSAGYYDAYYLKAQKVRTLIKEDFEKAFESVDVLVCPTT
PTTAFKAGEKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDDQGLPIGLQMIGNVLREDQVLQAAYAYEQSTEWHKASP
KL
>Mature_481_residues
ASIQALHQELITKERSAQEITEAALETIHQLEPKLHSFLAITADQALAQAKQVDAQLAAGEEIGLLAGIPIGIKDNMCTK
GIATTCGSKILQNFIPPYESTVTQKLAAAGAVMVGKTNLDEFAMGSSTENSAYQVTGNPWDVSRVPGGSSGGSAAAVAAD
ECVVSIGSDTGGSIRQPAALCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQEIAGYDPQDSTSLKVEI
PDYSKSLIPDLKGKKVGVITETFGEGLDEVVEKVVRKAIDQLAELGAEVQEISCPRFRYGLPTYYVIAPSEASANLARYD
GVKYGYRTDDPDDLMSMYTNTRAEGFGAEVKRRIMIGTYALSAGYYDAYYLKAQKVRTLIKEDFEKAFESVDVLVCPTTP
TTAFKAGEKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDDQGLPIGLQMIGNVLREDQVLQAAYAYEQSTEWHKASPK
L

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=482, Percent_Identity=39.4190871369295, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI195972892, Length=481, Percent_Identity=24.948024948025, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI166795287, Length=167, Percent_Identity=34.1317365269461, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=438, Percent_Identity=38.1278538812785, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=509, Percent_Identity=26.5225933202358, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=245, Percent_Identity=36.3265306122449, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=237, Percent_Identity=30.8016877637131, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=190, Percent_Identity=36.8421052631579, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=190, Percent_Identity=36.8421052631579, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=125, Percent_Identity=32, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=166, Percent_Identity=31.9277108433735, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=419, Percent_Identity=36.9928400954654, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=479, Percent_Identity=28.6012526096033, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=227, Percent_Identity=32.15859030837, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648113, Length=492, Percent_Identity=40.4471544715447, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652985, Length=507, Percent_Identity=25.6410256410256, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI19922090, Length=507, Percent_Identity=25.6410256410256, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24652981, Length=507, Percent_Identity=25.6410256410256, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24652983, Length=507, Percent_Identity=25.6410256410256, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=26.3052208835341, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550774, Length=477, Percent_Identity=23.2704402515723, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648435, Length=477, Percent_Identity=23.2704402515723, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648437, Length=477, Percent_Identity=23.2704402515723, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078093, Length=497, Percent_Identity=25.3521126760563, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356731, Length=492, Percent_Identity=23.3739837398374, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648441, Length=421, Percent_Identity=23.2779097387173, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648439, Length=421, Percent_Identity=23.2779097387173, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_ACAM1 (B0C269)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517837.1
- ProteinModelPortal:   B0C269
- SMR:   B0C269
- GeneID:   5682337
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3531
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   AMAR329726:AM1_3531-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51516; Mature: 51385

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 75-75 ACT_SITE 150-150 ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIQALHQELITKERSAQEITEAALETIHQLEPKLHSFLAITADQALAQAKQVDAQLAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEEIGLLAGIPIGIKDNMCTKGIATTCGSKILQNFIPPYESTVTQKLAAAGAVMVGKTNL
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCH
DEFAMGSSTENSAYQVTGNPWDVSRVPGGSSGGSAAAVAADECVVSIGSDTGGSIRQPAA
HHHHCCCCCCCCEEEEECCCEECCCCCCCCCCCCEEEEEHHHEEEEECCCCCCCCCCCHH
LCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQEIAGYDPQDSTSLKVE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
IPDYSKSLIPDLKGKKVGVITETFGEGLDEVVEKVVRKAIDQLAELGAEVQEISCPRFRY
CCCCCHHHCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEC
GLPTYYVIAPSEASANLARYDGVKYGYRTDDPDDLMSMYTNTRAEGFGAEVKRRIMIGTY
CCCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHEEEEE
ALSAGYYDAYYLKAQKVRTLIKEDFEKAFESVDVLVCPTTPTTAFKAGEKTADPLSMYLS
EHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHCCCCCCCCHHHHHHH
DLMTIPVNLAGLPGLSLPCGFDDQGLPIGLQMIGNVLREDQVLQAAYAYEQSTEWHKASP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KL
CC
>Mature Secondary Structure 
ASIQALHQELITKERSAQEITEAALETIHQLEPKLHSFLAITADQALAQAKQVDAQLAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEEIGLLAGIPIGIKDNMCTKGIATTCGSKILQNFIPPYESTVTQKLAAAGAVMVGKTNL
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCH
DEFAMGSSTENSAYQVTGNPWDVSRVPGGSSGGSAAAVAADECVVSIGSDTGGSIRQPAA
HHHHCCCCCCCCEEEEECCCEECCCCCCCCCCCCEEEEEHHHEEEEECCCCCCCCCCCHH
LCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQEIAGYDPQDSTSLKVE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
IPDYSKSLIPDLKGKKVGVITETFGEGLDEVVEKVVRKAIDQLAELGAEVQEISCPRFRY
CCCCCHHHCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEC
GLPTYYVIAPSEASANLARYDGVKYGYRTDDPDDLMSMYTNTRAEGFGAEVKRRIMIGTY
CCCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHEEEEE
ALSAGYYDAYYLKAQKVRTLIKEDFEKAFESVDVLVCPTTPTTAFKAGEKTADPLSMYLS
EHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHCCCCCCCCHHHHHHH
DLMTIPVNLAGLPGLSLPCGFDDQGLPIGLQMIGNVLREDQVLQAAYAYEQSTEWHKASP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA