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The map label for this gene is speE [H]

Identifier: 158336659

GI number: 158336659

Start: 3565478

End: 3566428

Strand: Direct

Name: speE [H]

Synonym: AM1_3527

Alternate gene names: 158336659

Gene position: 3565478-3566428 (Clockwise)

Preceding gene: 158336658

Following gene: 158336660

Centisome position: 54.82

GC content: 48.05

Gene sequence:

>951_bases
TTGCTGGAAACAGAAAATGTTGACTTCAAGCGTCCCGAAGAGGTCGCTGCCTACGAATATTCGGATCCTCAGGAAAAACG
CACCGTCTGGTTTACGGATCGCAATGACAATATTGCCTTATCCATTCGACATCGAGGCGGCTGGATTTTCCGGGAAAAGT
CGCCCTACCAAACCGTCGAAGTGCTCGATACCTTTGAATACGGCAAGATGCTGGTGATCGACAATATGGTCATGTGCAGT
GAAGGGGATGAAAAAGCCTATCACGAGATGATTGTCCATGTGCCCATGCTCACTTATTCCGCTCGAAAAGTGCTGGTGAT
TGGTGGTGGTGATGGCGGTAGCGTTCGGGAAATTTTGCGTTATCCTCAAGTTGAAGAGGTCACCCTAGTCGAAATTGATG
AGGTGGTGATCAAGGCTGCTAGAGAACATCTACCCACCTTGTCTTCAGCCCTAGACCACCCCAAACTGAATCTACTGATT
GGCGATGGTATCGAGTATTTAGCAGCAGCTGCCGATGAGTCCTATGACCTGATTATTATTGATTCCTCTGACCCCGTTGG
ATGTTCTGCGCCCCTGTTTACCAAGGAATTTTATCTCCAGGTACACCGAGTTTTAAGCTCAGGTGGAGTTCTGGTGGCCC
AAAGCGAGTCTCCCCGGTTTAATCAACCCAAATTTGTGGAACTTTATCAGGACTTAGGCGCGATTTTTGGGGCTTCTCAA
ATTCACTGTTATCTAGCTTTTATTTCTACTTACCCCACTGGGATGTGGAGCTTTGCCTACTGCTCCAAGAATGGTCCACA
TCCCCTCCAAGACTTCGACTTCCAAAAAGCCGAGCAGTTTTCCCAAGACCATCAATTGGCCTATTACAATCCTGGGATTC
ACCAAGCCGCTTTCTGTTTACCCACCTTTGTGAAAGACATGATTACGGTGGAGCAGCCTGTGCTGGTCTAG

Upstream 100 bases:

>100_bases
TGGCATGTCCGTTAACCCCTGGATTGCCTATGAAATCCTCAAGGAAGCCTTTCAAGCCGATCATGGCTCTGCTCTGGAGT
TAAACCGAGGCCAACTCCAT

Downstream 100 bases:

>100_bases
ACCAATGACGGCCTTTGATCCTAATGGCGTGGGCCTAGAAAATGGCAATCTGTTTGGCCTGCCCTTCGACTTTGACAGTG
CCCAGATTATCGTTTGGGGC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLETENVDFKRPEEVAAYEYSDPQEKRTVWFTDRNDNIALSIRHRGGWIFREKSPYQTVEVLDTFEYGKMLVIDNMVMCS
EGDEKAYHEMIVHVPMLTYSARKVLVIGGGDGGSVREILRYPQVEEVTLVEIDEVVIKAAREHLPTLSSALDHPKLNLLI
GDGIEYLAAAADESYDLIIIDSSDPVGCSAPLFTKEFYLQVHRVLSSGGVLVAQSESPRFNQPKFVELYQDLGAIFGASQ
IHCYLAFISTYPTGMWSFAYCSKNGPHPLQDFDFQKAEQFSQDHQLAYYNPGIHQAAFCLPTFVKDMITVEQPVLV

Sequences:

>Translated_316_residues
MLETENVDFKRPEEVAAYEYSDPQEKRTVWFTDRNDNIALSIRHRGGWIFREKSPYQTVEVLDTFEYGKMLVIDNMVMCS
EGDEKAYHEMIVHVPMLTYSARKVLVIGGGDGGSVREILRYPQVEEVTLVEIDEVVIKAAREHLPTLSSALDHPKLNLLI
GDGIEYLAAAADESYDLIIIDSSDPVGCSAPLFTKEFYLQVHRVLSSGGVLVAQSESPRFNQPKFVELYQDLGAIFGASQ
IHCYLAFISTYPTGMWSFAYCSKNGPHPLQDFDFQKAEQFSQDHQLAYYNPGIHQAAFCLPTFVKDMITVEQPVLV
>Mature_316_residues
MLETENVDFKRPEEVAAYEYSDPQEKRTVWFTDRNDNIALSIRHRGGWIFREKSPYQTVEVLDTFEYGKMLVIDNMVMCS
EGDEKAYHEMIVHVPMLTYSARKVLVIGGGDGGSVREILRYPQVEEVTLVEIDEVVIKAAREHLPTLSSALDHPKLNLLI
GDGIEYLAAAADESYDLIIIDSSDPVGCSAPLFTKEFYLQVHRVLSSGGVLVAQSESPRFNQPKFVELYQDLGAIFGASQ
IHCYLAFISTYPTGMWSFAYCSKNGPHPLQDFDFQKAEQFSQDHQLAYYNPGIHQAAFCLPTFVKDMITVEQPVLV

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=298, Percent_Identity=35.9060402684564, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1786312, Length=267, Percent_Identity=34.8314606741573, Blast_Score=174, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI25154106, Length=307, Percent_Identity=36.8078175895765, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI212645616, Length=263, Percent_Identity=35.7414448669202, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6325326, Length=283, Percent_Identity=36.7491166077738, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323175, Length=267, Percent_Identity=35.5805243445693, Blast_Score=170, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24645443, Length=258, Percent_Identity=33.7209302325581, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21355941, Length=217, Percent_Identity=33.1797235023041, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24663238, Length=149, Percent_Identity=26.8456375838926, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21358269, Length=149, Percent_Identity=26.8456375838926, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 35708; Mature: 35708

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2 ; PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETENVDFKRPEEVAAYEYSDPQEKRTVWFTDRNDNIALSIRHRGGWIFREKSPYQTVE
CCCCCCCCCCCCCHHEEECCCCCCCCCEEEEEECCCCEEEEEEECCCEEEECCCCCHHHH
VLDTFEYGKMLVIDNMVMCSEGDEKAYHEMIVHVPMLTYSARKVLVIGGGDGGSVREILR
HHHHHCCCCEEEEECEEEECCCCHHHHHHHHHHHHHEEECCCEEEEEECCCCHHHHHHHH
YPQVEEVTLVEIDEVVIKAAREHLPTLSSALDHPKLNLLIGDGIEYLAAAADESYDLIII
CCCCCCEEEEHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCCHHHHHHHCCCCEEEEEE
DSSDPVGCSAPLFTKEFYLQVHRVLSSGGVLVAQSESPRFNQPKFVELYQDLGAIFGASQ
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCH
IHCYLAFISTYPTGMWSFAYCSKNGPHPLQDFDFQKAEQFSQDHQLAYYNPGIHQAAFCL
HEEEEEEEHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCEEEECCCHHHHHHHH
PTFVKDMITVEQPVLV
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLETENVDFKRPEEVAAYEYSDPQEKRTVWFTDRNDNIALSIRHRGGWIFREKSPYQTVE
CCCCCCCCCCCCCHHEEECCCCCCCCCEEEEEECCCCEEEEEEECCCEEEECCCCCHHHH
VLDTFEYGKMLVIDNMVMCSEGDEKAYHEMIVHVPMLTYSARKVLVIGGGDGGSVREILR
HHHHHCCCCEEEEECEEEECCCCHHHHHHHHHHHHHEEECCCEEEEEECCCCHHHHHHHH
YPQVEEVTLVEIDEVVIKAAREHLPTLSSALDHPKLNLLIGDGIEYLAAAADESYDLIII
CCCCCCEEEEHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCCHHHHHHHCCCCEEEEEE
DSSDPVGCSAPLFTKEFYLQVHRVLSSGGVLVAQSESPRFNQPKFVELYQDLGAIFGASQ
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCH
IHCYLAFISTYPTGMWSFAYCSKNGPHPLQDFDFQKAEQFSQDHQLAYYNPGIHQAAFCL
HEEEEEEEHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCEEEECCCHHHHHHHH
PTFVKDMITVEQPVLV
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA