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The map label for this gene is ams [H]

Identifier: 158336602

GI number: 158336602

Start: 3502228

End: 3504174

Strand: Reverse

Name: ams [H]

Synonym: AM1_3468

Alternate gene names: 158336602

Gene position: 3504174-3502228 (Counterclockwise)

Preceding gene: 158336603

Following gene: 158336601

Centisome position: 53.88

GC content: 48.59

Gene sequence:

>1947_bases
ATGGATGCTGTAGCTCTCAAGCGACACAGTATCAAGTCTCTATCCCGGTTAATGCCCCGTTTAGAAGTTCGCTATGCGGA
CCAAATTGATCAAGGGACGTGGCAAAGCTATGTTCAGCGCATTAAAACCCACTTCCCCCGTTTATTTGGCTTGCTGTACC
AGCTGTATGGCCATCAGTATGACTTTTTCTATCATCTAGAACAGATTTTAGATTCAACCACCCATATGTGGATGAAGCGG
CCCGATGAACTGAAGGCCTTGGATGTGATGCGAGAAGCAGATCCCCACTGGTATCAGTCCCATCGGATGGTGGGGGCCAT
GTGTTACGTCGATCTCTTTGCCGGTGATTTGAGTGGGATTCGCGATCGCATCCCGTATCTCACGGAGCTAGGGATCACCT
ACCTCCATCTGATGCCTTTATTCAAAACCCTAGAAGGCGACAATGATGGGGGCTATGCCGTCAGTAGCTATCGCGAGGTC
GATCCCTCCCTCGGCACCATGGAGGAACTGGCTGATCTCGCCACTGAACTACGGCAACATGGCATTAGTCTGGTCCTGGA
CTTCGTGTTTAACCATACGTCCGATGAGCATCAATGGGCCAAGCAAGCTATGGCAGGCGATCGGGAGCATCAAGAGTACT
ACCGGATGTATCGAGATCGCGAGCTACCCGATCAGTTTGAACAAACGGTTAATTCAGTTTTTCCCGATGAGCATCCGGGT
TGTTTTACCTACCGCACCAAGATTCGCAAATGGATTTGGACGACGTTTTGTAATTACCAATGGGATCTCAATTACGAAAA
TCCCACGGTTTTTAGCCATATGGCTGAGGAAATGCTATTCCTGGCCAATGTGGGGGTCGAAGTGTTACGGCTAGATGCCG
TGGCATTTTTATGGAAGCGCTTAGGAAGTACGTGTGAGAATCTTCCAGAAGCCCATTTACTGATTCAAGCCTTCAATGTG
GTGTTGCAAATCGCGGCTCCAGCAATGGTTTTCAAATCAGAAGCGATCGTCCACCCCGATGAAGTCAGACGCTATATCAG
TGAAGAGGAATGTCCGCTGTCTTACAATCCACAGTTGATGGCCCTGCTATGGGAAGCGCTAGCAACCCGGAATACTCAAG
TGTTGCGCCATGCCATGCAGAAGCGATTTGACCTGCCAGAAGGTTGTGCTTGGGTCAACTATATTCGATGCCATGACGAT
ATTGGCTGGACCTTTTCGGATAATGATGCTTGGGAACTCAATATCAATCCGTTGCACCATCGGCAGTTCTTGACAGCCTT
TTATACCAATCGATTTGAAAATAGTTTTGCCCGCGGCCTGCCTTTCCAAGAAAACCTAGAAACCCAACTTGCTCGTATTT
CTGGAACCACCGCTTCTCTATGTGGTCTTGAGAAAGGGCTCTATGAACAGGATTCAGCCCAAGTGGAGCTGGCCATCCGG
CGAATCTTGCTGCTACACGGCATTTTATTAACCATTGGAGGCATTCCCCTTCTTTATTTAGGAGATGAGCTAGGTATCCT
CAACGACTATGACTATGGTCAATCTCCCGAGAAGGATGGGGATAGTCGATGGACGCATCGACTTACGTTCGATTGGGAAC
GGGCTGACTCGCGCCGAAATTCAGAAGAAGTCTGGGGGAAAATCTATTTGGGGCTGTTGAGGCTGATTCATATTCGTCAG
CAAAACCGGGCTTTTACGGGTTCAGAAACAGTCGTGATGGATCCTGGCAATGATCATGTGTTTGGCTATTTCCGCCACCA
TGATGATCAAAGTGTGTTGGTGCTCGGCAACTTTACGGAAAGTGAGCAGGCCATTGTGGGGAGACGGTTACGGTTACTCG
GCATGCGCAAAACTTTTACAGATATTGTGTCAGGAACCACGATCACCGCGACCCAAAAGCTGATGCTAGATCCTTATCAA
TTTATGATTCTGATTGGCGTTCGATAA

Upstream 100 bases:

>100_bases
GGGTCAGCTGGACTCATAGGCGATCCATCCTCTTTCAGACATCGTGGATACCGATACACTATTGCCATATCACTCCATTA
CCATGCCGATGCAAGAAGCC

Downstream 100 bases:

>100_bases
TAGCTACCTACCCCTGACACCGTAAAAATATATCTTGTCGAGTTCTTGACATTCACTGCCTACGGTAAAGGCTGTTTCTT
TTGGCTTTATGCCACGTTTG

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MDAVALKRHSIKSLSRLMPRLEVRYADQIDQGTWQSYVQRIKTHFPRLFGLLYQLYGHQYDFFYHLEQILDSTTHMWMKR
PDELKALDVMREADPHWYQSHRMVGAMCYVDLFAGDLSGIRDRIPYLTELGITYLHLMPLFKTLEGDNDGGYAVSSYREV
DPSLGTMEELADLATELRQHGISLVLDFVFNHTSDEHQWAKQAMAGDREHQEYYRMYRDRELPDQFEQTVNSVFPDEHPG
CFTYRTKIRKWIWTTFCNYQWDLNYENPTVFSHMAEEMLFLANVGVEVLRLDAVAFLWKRLGSTCENLPEAHLLIQAFNV
VLQIAAPAMVFKSEAIVHPDEVRRYISEEECPLSYNPQLMALLWEALATRNTQVLRHAMQKRFDLPEGCAWVNYIRCHDD
IGWTFSDNDAWELNINPLHHRQFLTAFYTNRFENSFARGLPFQENLETQLARISGTTASLCGLEKGLYEQDSAQVELAIR
RILLLHGILLTIGGIPLLYLGDELGILNDYDYGQSPEKDGDSRWTHRLTFDWERADSRRNSEEVWGKIYLGLLRLIHIRQ
QNRAFTGSETVVMDPGNDHVFGYFRHHDDQSVLVLGNFTESEQAIVGRRLRLLGMRKTFTDIVSGTTITATQKLMLDPYQ
FMILIGVR

Sequences:

>Translated_648_residues
MDAVALKRHSIKSLSRLMPRLEVRYADQIDQGTWQSYVQRIKTHFPRLFGLLYQLYGHQYDFFYHLEQILDSTTHMWMKR
PDELKALDVMREADPHWYQSHRMVGAMCYVDLFAGDLSGIRDRIPYLTELGITYLHLMPLFKTLEGDNDGGYAVSSYREV
DPSLGTMEELADLATELRQHGISLVLDFVFNHTSDEHQWAKQAMAGDREHQEYYRMYRDRELPDQFEQTVNSVFPDEHPG
CFTYRTKIRKWIWTTFCNYQWDLNYENPTVFSHMAEEMLFLANVGVEVLRLDAVAFLWKRLGSTCENLPEAHLLIQAFNV
VLQIAAPAMVFKSEAIVHPDEVRRYISEEECPLSYNPQLMALLWEALATRNTQVLRHAMQKRFDLPEGCAWVNYIRCHDD
IGWTFSDNDAWELNINPLHHRQFLTAFYTNRFENSFARGLPFQENLETQLARISGTTASLCGLEKGLYEQDSAQVELAIR
RILLLHGILLTIGGIPLLYLGDELGILNDYDYGQSPEKDGDSRWTHRLTFDWERADSRRNSEEVWGKIYLGLLRLIHIRQ
QNRAFTGSETVVMDPGNDHVFGYFRHHDDQSVLVLGNFTESEQAIVGRRLRLLGMRKTFTDIVSGTTITATQKLMLDPYQ
FMILIGVR
>Mature_648_residues
MDAVALKRHSIKSLSRLMPRLEVRYADQIDQGTWQSYVQRIKTHFPRLFGLLYQLYGHQYDFFYHLEQILDSTTHMWMKR
PDELKALDVMREADPHWYQSHRMVGAMCYVDLFAGDLSGIRDRIPYLTELGITYLHLMPLFKTLEGDNDGGYAVSSYREV
DPSLGTMEELADLATELRQHGISLVLDFVFNHTSDEHQWAKQAMAGDREHQEYYRMYRDRELPDQFEQTVNSVFPDEHPG
CFTYRTKIRKWIWTTFCNYQWDLNYENPTVFSHMAEEMLFLANVGVEVLRLDAVAFLWKRLGSTCENLPEAHLLIQAFNV
VLQIAAPAMVFKSEAIVHPDEVRRYISEEECPLSYNPQLMALLWEALATRNTQVLRHAMQKRFDLPEGCAWVNYIRCHDD
IGWTFSDNDAWELNINPLHHRQFLTAFYTNRFENSFARGLPFQENLETQLARISGTTASLCGLEKGLYEQDSAQVELAIR
RILLLHGILLTIGGIPLLYLGDELGILNDYDYGQSPEKDGDSRWTHRLTFDWERADSRRNSEEVWGKIYLGLLRLIHIRQ
QNRAFTGSETVVMDPGNDHVFGYFRHHDDQSVLVLGNFTESEQAIVGRRLRLLGMRKTFTDIVSGTTITATQKLMLDPYQ
FMILIGVR

Specific function: Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose s

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=188, Percent_Identity=30.8510638297872, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI87081873, Length=521, Percent_Identity=25.7197696737044, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1790687, Length=212, Percent_Identity=32.0754716981132, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1786604, Length=88, Percent_Identity=43.1818181818182, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321726, Length=219, Percent_Identity=25.5707762557078, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322241, Length=219, Percent_Identity=25.5707762557078, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6322021, Length=219, Percent_Identity=25.5707762557078, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324416, Length=219, Percent_Identity=25.5707762557078, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321731, Length=206, Percent_Identity=25.7281553398058, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319776, Length=206, Percent_Identity=25.7281553398058, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=210, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24586587, Length=190, Percent_Identity=33.6842105263158, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583747, Length=193, Percent_Identity=36.2694300518135, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583749, Length=193, Percent_Identity=36.2694300518135, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24586589, Length=199, Percent_Identity=32.6633165829146, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45549022, Length=194, Percent_Identity=29.3814432989691, Blast_Score=100, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24586591, Length=200, Percent_Identity=29.5, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24586593, Length=188, Percent_Identity=32.9787234042553, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583745, Length=194, Percent_Identity=30.4123711340206, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221330053, Length=208, Percent_Identity=28.3653846153846, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24586599, Length=206, Percent_Identity=29.126213592233, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24586597, Length=194, Percent_Identity=28.8659793814433, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281360393, Length=153, Percent_Identity=30.0653594771242, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.4 [H]

Molecular weight: Translated: 75485; Mature: 75485

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCEEEEEEEECC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12517860 [H]