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The map label for this gene is dapA

Identifier: 158336582

GI number: 158336582

Start: 3474655

End: 3475539

Strand: Reverse

Name: dapA

Synonym: AM1_3445

Alternate gene names: 158336582

Gene position: 3475539-3474655 (Counterclockwise)

Preceding gene: 158336583

Following gene: 158336580

Centisome position: 53.44

GC content: 47.01

Gene sequence:

>885_bases
GTGACCTTCTTCGGACAGATTCTGACAGCCATGGTTACCCCCTTTACTGACACTGGGGACATGAATTTTTCTGTGGCCGA
AGCTCTAGCCATTCATCTTGCCGATCATGGCACAGATACGCTTTTATTGTGCGGTACGACAGGTGAGTCTCCAACCTTAA
CCTGGCAAGAAGAATATGAGCTATTTCAAGTGGTTCGTAAAGCGGTTGCCAGCAAAGCTAAAGTGATGGCGGGAACTGGA
TCTAATTCCACCCGAGAAGCCATTGAAGCCACGCAACAAGCTGCAAAGTTAGGACTAGATGGTGCCTTGTTGGTTGTGCC
ATACTATAACAAGCCACCCCAAGAAGGTTTATACGAACATTTTCGGGCCATTGCGGAGTCTGTTCCTGATTTCCCGTTGA
TGTTATACAACATACCGGGTCGCACGGGGCAGAATTTGGAAGCTGACACAGTCGCTCGGCTGGCAGAAATTGGCAATATC
GTGGCCATTAAAGAAGCGAGTGGCAACCTTGATCAAGTCAGTCAAATTCGTCGCTTAACACCACCTGAGTTTTCCATTTA
CTCTGGAGATGACTCTCTGACCTTACCTTATCTATCCGTTGGTGCTGCAGGCGTAGTCAGTGTAGCCAGTCATTTGGTGG
GAGAGTTGCTTCAAAAAATGGTGACTGCGTTTGCGTCAGGGGAACCACAAGTCGCAACAGAGATTCACCTTAAATTGCTC
CCTTTATGTAAAGCATTGTTCGCAACCACTAATCCTATCCCAGTCAAAGCTGCACTTCAATTACAAGGATGGCAAGTGGG
TTCTACCCGTTTACCGCTTCCTGAAGCGCCTGACCCCATCATTCAAAAATTGAAAAATACTCTAACAGATTTGGCATTGC
TATAG

Upstream 100 bases:

>100_bases
AAGCATCCACGCAGCTTTTTACGCTGCCTTAACTATGAATACTGGAGTGTGCCATTTCTCACATGAGCCTATTGAACCTT
AATATCGGAGCGGACAGCGC

Downstream 100 bases:

>100_bases
GCATGGAGCACCTCTACTAACCCAACGCTCCACAACAAAATCCATCGCACTTCAATGCAGACAGTCCATTGCACAGTCTG
CCCACTATCTAGCACAAGAC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTFFGQILTAMVTPFTDTGDMNFSVAEALAIHLADHGTDTLLLCGTTGESPTLTWQEEYELFQVVRKAVASKAKVMAGTG
SNSTREAIEATQQAAKLGLDGALLVVPYYNKPPQEGLYEHFRAIAESVPDFPLMLYNIPGRTGQNLEADTVARLAEIGNI
VAIKEASGNLDQVSQIRRLTPPEFSIYSGDDSLTLPYLSVGAAGVVSVASHLVGELLQKMVTAFASGEPQVATEIHLKLL
PLCKALFATTNPIPVKAALQLQGWQVGSTRLPLPEAPDPIIQKLKNTLTDLALL

Sequences:

>Translated_294_residues
MTFFGQILTAMVTPFTDTGDMNFSVAEALAIHLADHGTDTLLLCGTTGESPTLTWQEEYELFQVVRKAVASKAKVMAGTG
SNSTREAIEATQQAAKLGLDGALLVVPYYNKPPQEGLYEHFRAIAESVPDFPLMLYNIPGRTGQNLEADTVARLAEIGNI
VAIKEASGNLDQVSQIRRLTPPEFSIYSGDDSLTLPYLSVGAAGVVSVASHLVGELLQKMVTAFASGEPQVATEIHLKLL
PLCKALFATTNPIPVKAALQLQGWQVGSTRLPLPEAPDPIIQKLKNTLTDLALL
>Mature_293_residues
TFFGQILTAMVTPFTDTGDMNFSVAEALAIHLADHGTDTLLLCGTTGESPTLTWQEEYELFQVVRKAVASKAKVMAGTGS
NSTREAIEATQQAAKLGLDGALLVVPYYNKPPQEGLYEHFRAIAESVPDFPLMLYNIPGRTGQNLEADTVARLAEIGNIV
AIKEASGNLDQVSQIRRLTPPEFSIYSGDDSLTLPYLSVGAAGVVSVASHLVGELLQKMVTAFASGEPQVATEIHLKLLP
LCKALFATTNPIPVKAALQLQGWQVGSTRLPLPEAPDPIIQKLKNTLTDLALL

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:
GO:0003824: Dihydrodipicolinate synthase
GO:0005737: Dihydrodipicolinate synthase
GO:0008152: Dihydrodipicolinate synthase
GO:0008652: Dihydrodipicolinate synthase
GO:0008840: Dihydrodipicolinate synthase
GO:0009085: Dihydrodipicolinate synthase
GO:0009089: Dihydrodipicolinate synthase
GO:0016829: Dihydrodipicolinate synthase
GO:0019877: Dihydrodipicolinate synthase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=272, Percent_Identity=26.1029411764706, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI13540533, Length=296, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788823, Length=294, Percent_Identity=40.4761904761905, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI87082415, Length=281, Percent_Identity=30.6049822064057, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789620, Length=243, Percent_Identity=28.3950617283951, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1786463, Length=284, Percent_Identity=29.5774647887324, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_ACAM1 (B0C142)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517756.1
- ProteinModelPortal:   B0C142
- SMR:   B0C142
- GeneID:   5682254
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3445
- HOGENOM:   HBG358848
- OMA:   LFITTNP
- ProtClustDB:   PRK03170
- BioCyc:   AMAR329726:AM1_3445-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31515; Mature: 31383

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 164-164 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFFGQILTAMVTPFTDTGDMNFSVAEALAIHLADHGTDTLLLCGTTGESPTLTWQEEYE
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEHHHHHH
LFQVVRKAVASKAKVMAGTGSNSTREAIEATQQAAKLGLDGALLVVPYYNKPPQEGLYEH
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
FRAIAESVPDFPLMLYNIPGRTGQNLEADTVARLAEIGNIVAIKEASGNLDQVSQIRRLT
HHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCC
PPEFSIYSGDDSLTLPYLSVGAAGVVSVASHLVGELLQKMVTAFASGEPQVATEIHLKLL
CCCCEEECCCCCEECCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
PLCKALFATTNPIPVKAALQLQGWQVGSTRLPLPEAPDPIIQKLKNTLTDLALL
HHHHHHHHCCCCCCEEEEEEEECEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFFGQILTAMVTPFTDTGDMNFSVAEALAIHLADHGTDTLLLCGTTGESPTLTWQEEYE
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEHHHHHH
LFQVVRKAVASKAKVMAGTGSNSTREAIEATQQAAKLGLDGALLVVPYYNKPPQEGLYEH
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
FRAIAESVPDFPLMLYNIPGRTGQNLEADTVARLAEIGNIVAIKEASGNLDQVSQIRRLT
HHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCC
PPEFSIYSGDDSLTLPYLSVGAAGVVSVASHLVGELLQKMVTAFASGEPQVATEIHLKLL
CCCCEEECCCCCEECCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
PLCKALFATTNPIPVKAALQLQGWQVGSTRLPLPEAPDPIIQKLKNTLTDLALL
HHHHHHHHCCCCCCEEEEEEEECEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA