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The map label for this gene is mrdA [H]

Identifier: 158336573

GI number: 158336573

Start: 3466152

End: 3467921

Strand: Direct

Name: mrdA [H]

Synonym: AM1_3437

Alternate gene names: 158336573

Gene position: 3466152-3467921 (Clockwise)

Preceding gene: 158336571

Following gene: 158336574

Centisome position: 53.29

GC content: 50.45

Gene sequence:

>1770_bases
ATGTCCCTTTCTAATCAATCATTTTCTCTACCACGACGTCAGCGTTCTCAGCGCAGTGTCGGATCTGGCCCTCGGAAGTT
TGTTTTTGGTGGCTTTATCAGCCTTTTATTGTTTGGAGCGATTGTTCCGCGCCTCAGTTTTTTGCAGATTACTGAAGGGG
CACTGAATCTTCAGAGAGCCGAAGAAAATCGAGTTCGTTTAATTCCAAAACGGCCTGAACGGGGCAAAATCCTAGATCGT
AAAGGCCGGATTCTGGCGAACAGCACCTATACCTATTCCGTTTTTGTTTGGCCCATTGCTCAAAAAGACGAAAAGTGGTC
CCAAACGGTGGATGTCCTGTCTTCAATTCTTAAAATTCCTGAAGCGGAAATTCAGGAGCGCGTAGAGGTAGAAGGCCATA
ACTCAACTTCCTTAGTCCGCGTGGCTCAAGGCTTGACCTTTCCCCAAGTGGTGGCGCTGTCGGAACGGATGAGCGACATT
GTAGGGGTTGAAATTGATAAGGAAGCCATTCGCTATTACCCCCATGGCGAACTGGCCTCTCAGGTAATTGGCTATATCGG
CGAGATTAATGAAGAAGAGTTAGCTCGCCAACAAAAGAAGCCCTATCGCCTAGGGGATGTGGTTGGCCAATTAGGGATTG
AAGCCTCCTACGAACAAAAGCTACGGGGCACTTGGGGGGGCAACCAAATTGAGGTGGATGGTGCAGGCCGGATTGTGCAA
ATCTTGGGCCAAAAATTACCCATCGCTGGCGAGGATGTCAAACTCACCCTTGATTTAGACGTGCAAAAGGCAGCCGAAAA
GGCCTTAGGCAAACGGCAAGGGGCGGTGGTCGCCATCAATCCTAAGAATGGCGCTATTATTGCTATGGCCAGCTATCCAG
GATTTAACCCCAACTGGTTTGCCAAGCGCGTTACAGAAAAGCAATGGCAAGAGTTACAAAACCGCAAATTCCCCTTTGTC
AATCGGGCCATGCAGGCCTTTCCCCCTGCGAGTACTTTCAAGATCGCCACTGCGATCGCAGGAATTGAATCCGGTAAATA
TTCCCCCAATGCTCTGGTAGCCACCTCAGCCTCAGTGCATGGTGTGGGAGATTGGAATGGGGCAGGCTTTGGCGTGATTG
GTTTTCAAACGGCCTTGCAGTGGAGTAGCAATACCTTTTTTGGGCGTGTGGGCGTGGGGACCAGTCCCAAAATTCTAATT
GAGTGGGCTAAGCGTTTAGGGGTTGGAGAGAGAACGGGCATTGATATCCCAGGAGAGTCTTCTGGGTTTATTCCTGATCC
AGCTTGGAAAAAAGAGGTGTTTAAAGACGCCTGGTATCCCGCTGACACCGTTATGGTTTCTATCGGGCAGGGGGCAGTAC
AACTCAGTCCCCTCCAAGTCTCCCTCATTTTTGCTGCCATTGCCAATGGGGGGTATAAAGTCACCCCTCACCTATTCCAG
TCCGATCAGCCCGATGACCAAGGGCGGACCTCCCTGAATTTAAAACCCAAGACCTTAAATACGATTCGTTCGGGTTTACG
AGCGGTGGTCACCAGCGGTACAGGTCAGGCGTTAAATGTGCCTAGTATTCCCCCGGCAGCGGGTAAAAGTGGGACGGCTG
AAGACCCCCCTCGCTCTTCCCACACTTGGTTTGGGGCCTATGCTCCCTTTAATAACCCGGAAATTGTAGTGGTGGCCTTT
GGTGAAAATACAGGTGGGGGTGGGGGATCTACGGCAGGTCCTATTGCCTTAAAAGTCTTGGAAGCCTACTTTAAGGCTCA
GAAGAAATAG

Upstream 100 bases:

>100_bases
AGAATATCGCGGTAGCCTTAGCTAGAGCTTTTTTCGAAGATTAGGCGCTTCCTACCGATGTCATGCGGATTATGCGAGTG
TGCCCTATCCAACAGGATCA

Downstream 100 bases:

>100_bases
CCCTCATTAAGGATCGTTGACGACCATTGCCAGTTTTTTTTGGGCAACTCTACAATTGAGGGGCAATGATGTCGTCTTGC
TTTGGCAAGGCAAGGAAGAC

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MSLSNQSFSLPRRQRSQRSVGSGPRKFVFGGFISLLLFGAIVPRLSFLQITEGALNLQRAEENRVRLIPKRPERGKILDR
KGRILANSTYTYSVFVWPIAQKDEKWSQTVDVLSSILKIPEAEIQERVEVEGHNSTSLVRVAQGLTFPQVVALSERMSDI
VGVEIDKEAIRYYPHGELASQVIGYIGEINEEELARQQKKPYRLGDVVGQLGIEASYEQKLRGTWGGNQIEVDGAGRIVQ
ILGQKLPIAGEDVKLTLDLDVQKAAEKALGKRQGAVVAINPKNGAIIAMASYPGFNPNWFAKRVTEKQWQELQNRKFPFV
NRAMQAFPPASTFKIATAIAGIESGKYSPNALVATSASVHGVGDWNGAGFGVIGFQTALQWSSNTFFGRVGVGTSPKILI
EWAKRLGVGERTGIDIPGESSGFIPDPAWKKEVFKDAWYPADTVMVSIGQGAVQLSPLQVSLIFAAIANGGYKVTPHLFQ
SDQPDDQGRTSLNLKPKTLNTIRSGLRAVVTSGTGQALNVPSIPPAAGKSGTAEDPPRSSHTWFGAYAPFNNPEIVVVAF
GENTGGGGGSTAGPIALKVLEAYFKAQKK

Sequences:

>Translated_589_residues
MSLSNQSFSLPRRQRSQRSVGSGPRKFVFGGFISLLLFGAIVPRLSFLQITEGALNLQRAEENRVRLIPKRPERGKILDR
KGRILANSTYTYSVFVWPIAQKDEKWSQTVDVLSSILKIPEAEIQERVEVEGHNSTSLVRVAQGLTFPQVVALSERMSDI
VGVEIDKEAIRYYPHGELASQVIGYIGEINEEELARQQKKPYRLGDVVGQLGIEASYEQKLRGTWGGNQIEVDGAGRIVQ
ILGQKLPIAGEDVKLTLDLDVQKAAEKALGKRQGAVVAINPKNGAIIAMASYPGFNPNWFAKRVTEKQWQELQNRKFPFV
NRAMQAFPPASTFKIATAIAGIESGKYSPNALVATSASVHGVGDWNGAGFGVIGFQTALQWSSNTFFGRVGVGTSPKILI
EWAKRLGVGERTGIDIPGESSGFIPDPAWKKEVFKDAWYPADTVMVSIGQGAVQLSPLQVSLIFAAIANGGYKVTPHLFQ
SDQPDDQGRTSLNLKPKTLNTIRSGLRAVVTSGTGQALNVPSIPPAAGKSGTAEDPPRSSHTWFGAYAPFNNPEIVVVAF
GENTGGGGGSTAGPIALKVLEAYFKAQKK
>Mature_588_residues
SLSNQSFSLPRRQRSQRSVGSGPRKFVFGGFISLLLFGAIVPRLSFLQITEGALNLQRAEENRVRLIPKRPERGKILDRK
GRILANSTYTYSVFVWPIAQKDEKWSQTVDVLSSILKIPEAEIQERVEVEGHNSTSLVRVAQGLTFPQVVALSERMSDIV
GVEIDKEAIRYYPHGELASQVIGYIGEINEEELARQQKKPYRLGDVVGQLGIEASYEQKLRGTWGGNQIEVDGAGRIVQI
LGQKLPIAGEDVKLTLDLDVQKAAEKALGKRQGAVVAINPKNGAIIAMASYPGFNPNWFAKRVTEKQWQELQNRKFPFVN
RAMQAFPPASTFKIATAIAGIESGKYSPNALVATSASVHGVGDWNGAGFGVIGFQTALQWSSNTFFGRVGVGTSPKILIE
WAKRLGVGERTGIDIPGESSGFIPDPAWKKEVFKDAWYPADTVMVSIGQGAVQLSPLQVSLIFAAIANGGYKVTPHLFQS
DQPDDQGRTSLNLKPKTLNTIRSGLRAVVTSGTGQALNVPSIPPAAGKSGTAEDPPRSSHTWFGAYAPFNNPEIVVVAFG
ENTGGGGGSTAGPIALKVLEAYFKAQKK

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=586, Percent_Identity=32.0819112627986, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI1786272, Length=489, Percent_Identity=22.2903885480573, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 63811; Mature: 63680

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00337 BETA_LACTAMASE_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSNQSFSLPRRQRSQRSVGSGPRKFVFGGFISLLLFGAIVPRLSFLQITEGALNLQRA
CCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHC
EENRVRLIPKRPERGKILDRKGRILANSTYTYSVFVWPIAQKDEKWSQTVDVLSSILKIP
CCCCEEEECCCCCCCCEECCCCCEEECCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCC
EAEIQERVEVEGHNSTSLVRVAQGLTFPQVVALSERMSDIVGVEIDKEAIRYYPHGELAS
HHHHHHHHEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCEECHHHHHCCCCHHHHH
QVIGYIGEINEEELARQQKKPYRLGDVVGQLGIEASYEQKLRGTWGGNQIEVDGAGRIVQ
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCEEEECCCCHHHH
ILGQKLPIAGEDVKLTLDLDVQKAAEKALGKRQGAVVAINPKNGAIIAMASYPGFNPNWF
HHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCHH
AKRVTEKQWQELQNRKFPFVNRAMQAFPPASTFKIATAIAGIESGKYSPNALVATSASVH
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCC
GVGDWNGAGFGVIGFQTALQWSSNTFFGRVGVGTSPKILIEWAKRLGVGERTGIDIPGES
CCCCCCCCCEEEEEHHHHHEECCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
SGFIPDPAWKKEVFKDAWYPADTVMVSIGQGAVQLSPLQVSLIFAAIANGGYKVTPHLFQ
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHHHHCCCEEECCHHHC
SDQPDDQGRTSLNLKPKTLNTIRSGLRAVVTSGTGQALNVPSIPPAAGKSGTAEDPPRSS
CCCCCCCCCCEEECCCHHHHHHHHHHHEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCC
HTWFGAYAPFNNPEIVVVAFGENTGGGGGSTAGPIALKVLEAYFKAQKK
CCEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLSNQSFSLPRRQRSQRSVGSGPRKFVFGGFISLLLFGAIVPRLSFLQITEGALNLQRA
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHC
EENRVRLIPKRPERGKILDRKGRILANSTYTYSVFVWPIAQKDEKWSQTVDVLSSILKIP
CCCCEEEECCCCCCCCEECCCCCEEECCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCC
EAEIQERVEVEGHNSTSLVRVAQGLTFPQVVALSERMSDIVGVEIDKEAIRYYPHGELAS
HHHHHHHHEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCEECHHHHHCCCCHHHHH
QVIGYIGEINEEELARQQKKPYRLGDVVGQLGIEASYEQKLRGTWGGNQIEVDGAGRIVQ
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCEEEECCCCHHHH
ILGQKLPIAGEDVKLTLDLDVQKAAEKALGKRQGAVVAINPKNGAIIAMASYPGFNPNWF
HHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCHH
AKRVTEKQWQELQNRKFPFVNRAMQAFPPASTFKIATAIAGIESGKYSPNALVATSASVH
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCC
GVGDWNGAGFGVIGFQTALQWSSNTFFGRVGVGTSPKILIEWAKRLGVGERTGIDIPGES
CCCCCCCCCEEEEEHHHHHEECCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
SGFIPDPAWKKEVFKDAWYPADTVMVSIGQGAVQLSPLQVSLIFAAIANGGYKVTPHLFQ
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHHHHCCCEEECCHHHC
SDQPDDQGRTSLNLKPKTLNTIRSGLRAVVTSGTGQALNVPSIPPAAGKSGTAEDPPRSS
CCCCCCCCCCEEECCCHHHHHHHHHHHEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCC
HTWFGAYAPFNNPEIVVVAFGENTGGGGGSTAGPIALKVLEAYFKAQKK
CCEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]