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The map label for this gene is rsmE [H]

Identifier: 158336565

GI number: 158336565

Start: 3459636

End: 3460427

Strand: Direct

Name: rsmE [H]

Synonym: AM1_3429

Alternate gene names: 158336565

Gene position: 3459636-3460427 (Clockwise)

Preceding gene: 158336560

Following gene: 158336566

Centisome position: 53.19

GC content: 50.76

Gene sequence:

>792_bases
GTGGACCATAGTGCGGCCCAACTGCAGCGGTTAGCAATAGCCCCTGAGCAACTCCAAGATCATCAGATTCATTTGACGAG
TGATCAACAACATTATCTTTGTGATGTGTTGCGGCTGGGGCATGGCGATCGCTTTATTGCCATGAATGGGCAGGGACAAC
TCTGGGTAGCGGAGTTGCAACCTGATGCTAGGTTCGCCAGGCGGTTGAAAGCTTTAGGTGAGCAGACTGAGCTGGCGATG
CCGATGATGTTACTGGCTGCCCCTCCGAAAGGGAATCATTTTGACCAAGTGGTTCGGAGTGTAACGGAATTAGGGGTCAG
TTATATTGTGCCGCTCATTAGCCAGCGAACGCTGCTGAAACCGAGTTCTAATCGTATTCAACGATGGCAGCGGATTGCCA
CAGAAGCAGCTGAGCAGTCCCACCGGCAGATTATTCCTAAAGTGTTGGATCCGGCCTCCTTTGAAAAAACAGTGATGCAG
GATGATCAGGGCGACTGGCAACGATATATTTGTACTGTAGAGCCGTCAGCACCGAATTTCTTGCACTGTCTCAGCCAAGC
TTTAGATCAAGAGAGCCAATCAGGTGTTGCTGTTATGGTAGGACCCGAGGGTGGATGGACCGAGACTGAGACCCAGCAGG
CCCAAACAGCCGGATACCAAGCAGTATCTCTAGGCCAAAGAACCTTAAGAGCGGTCACCGCAAATTACATGGCAGTATCT
ATTGCGATCGCACAAATAGAACTACACACTGCGTACCCAGGGGGAAAAGTATGCACCAAGAAATCGCCATAG

Upstream 100 bases:

>100_bases
AAGATCGCTACTTCCCTTTTTTTGGGTTTAATAGTTTTTATATACCTGTAGCGATAGTTGCCATCGCAAAATTTCCAATA
CCTTAGGTAAACCTAAACTT

Downstream 100 bases:

>100_bases
CATTGGATCGTCAAGACTATCAGATGGCGTCTCAATTGATTCAAGCCATCCCCATGAGCGATCCATGGGGCAAGCTCTAT
TTGGGGAAATTGCAAGCGGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MDHSAAQLQRLAIAPEQLQDHQIHLTSDQQHYLCDVLRLGHGDRFIAMNGQGQLWVAELQPDARFARRLKALGEQTELAM
PMMLLAAPPKGNHFDQVVRSVTELGVSYIVPLISQRTLLKPSSNRIQRWQRIATEAAEQSHRQIIPKVLDPASFEKTVMQ
DDQGDWQRYICTVEPSAPNFLHCLSQALDQESQSGVAVMVGPEGGWTETETQQAQTAGYQAVSLGQRTLRAVTANYMAVS
IAIAQIELHTAYPGGKVCTKKSP

Sequences:

>Translated_263_residues
MDHSAAQLQRLAIAPEQLQDHQIHLTSDQQHYLCDVLRLGHGDRFIAMNGQGQLWVAELQPDARFARRLKALGEQTELAM
PMMLLAAPPKGNHFDQVVRSVTELGVSYIVPLISQRTLLKPSSNRIQRWQRIATEAAEQSHRQIIPKVLDPASFEKTVMQ
DDQGDWQRYICTVEPSAPNFLHCLSQALDQESQSGVAVMVGPEGGWTETETQQAQTAGYQAVSLGQRTLRAVTANYMAVS
IAIAQIELHTAYPGGKVCTKKSP
>Mature_263_residues
MDHSAAQLQRLAIAPEQLQDHQIHLTSDQQHYLCDVLRLGHGDRFIAMNGQGQLWVAELQPDARFARRLKALGEQTELAM
PMMLLAAPPKGNHFDQVVRSVTELGVSYIVPLISQRTLLKPSSNRIQRWQRIATEAAEQSHRQIIPKVLDPASFEKTVMQ
DDQGDWQRYICTVEPSAPNFLHCLSQALDQESQSGVAVMVGPEGGWTETETQQAQTAGYQAVSLGQRTLRAVTANYMAVS
IAIAQIELHTAYPGGKVCTKKSP

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=236, Percent_Identity=30.0847457627119, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 29176; Mature: 29176

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHSAAQLQRLAIAPEQLQDHQIHLTSDQQHYLCDVLRLGHGDRFIAMNGQGQLWVAELQ
CCCHHHHHHHHHCCHHHHCCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEECC
PDARFARRLKALGEQTELAMPMMLLAAPPKGNHFDQVVRSVTELGVSYIVPLISQRTLLK
CHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSSNRIQRWQRIATEAAEQSHRQIIPKVLDPASFEKTVMQDDQGDWQRYICTVEPSAPNF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHH
LHCLSQALDQESQSGVAVMVGPEGGWTETETQQAQTAGYQAVSLGQRTLRAVTANYMAVS
HHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEE
IAIAQIELHTAYPGGKVCTKKSP
EEEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MDHSAAQLQRLAIAPEQLQDHQIHLTSDQQHYLCDVLRLGHGDRFIAMNGQGQLWVAELQ
CCCHHHHHHHHHCCHHHHCCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEECC
PDARFARRLKALGEQTELAMPMMLLAAPPKGNHFDQVVRSVTELGVSYIVPLISQRTLLK
CHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSSNRIQRWQRIATEAAEQSHRQIIPKVLDPASFEKTVMQDDQGDWQRYICTVEPSAPNF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHH
LHCLSQALDQESQSGVAVMVGPEGGWTETETQQAQTAGYQAVSLGQRTLRAVTANYMAVS
HHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEE
IAIAQIELHTAYPGGKVCTKKSP
EEEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]