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The map label for this gene is yfmC [H]

Identifier: 158336537

GI number: 158336537

Start: 3432162

End: 3433223

Strand: Reverse

Name: yfmC [H]

Synonym: AM1_3401

Alternate gene names: 158336537

Gene position: 3433223-3432162 (Counterclockwise)

Preceding gene: 158336538

Following gene: 158336536

Centisome position: 52.79

GC content: 49.44

Gene sequence:

>1062_bases
ATGGCACGACTGAGATTGAAAACGATGGGCCTCCTGATGGCCTTGGCGATAGTGGGCATAGCCTGTACCCGTCCCACTCC
ATCCACCTCAACAGGAACGGACTGTCGGACCATTACCCATGATCTGGGTGAAACAGAGGTCTGTGGTCAACCGCAAAAGG
TGGTCACCATTGGTCCCAATTTATTGGAACTCTTACTAGCTCTTGAGGTACAGCCGATTGCCCATGCCGAGTATTTTCCC
TTCCCTACTCGCCAATTTGATCAGCCCGATCAGCAAATTCCCTATTTGGGAAAGTATCTGACAGGACAGCCAGACAATGT
GGGGACTGCTGAGAGCCCTTCTTTAGAAGCGATCGCAAAGTTGAAACCGGATCTGATCCTGGCAGACAGCATCAAGAATA
AAGATGAGTATAAATTGCTGTCTAAAATTGCGCCCACCTTACTGTTTGACTACAGCGGTGCAGGTTCTGCCTGGCAACCC
GATTTGAAAGCGCTTGGCCAAGCCCTCCAGAAAACTGAAAAAGCCACCGCAGTGACGGCGGAGTCTCAACGGCAAGTTGC
CAAATTAAAGCAAGATTTGCAGCCCATCGTGGCTAAGGATCCTAAAGTCTTGCTGCTATTGTCTGAGCAACTGAGCCAGG
GCGTGCGCCTAGAAACCGCAAACAGTGCCTGTGGGGCCTTGCTTGAAGACGTTGGGTTTCAGGTAATGGTCCCGGCTGCT
TTGAGCCAATCCCAAGAACCGAGTCATGTGATTTCTTTAGAATCGTTGACCAATCTGGATATGGACTGGATTTTGATCGA
AGGCTTCTCCAGCCAAAACCTTGATAAAACGTCCAACCCGGAGGAACAGCAGATCAAAGCTATTAAAACCGAGTGGAGCG
AGAATGCGATCGCACAATCACTACCACCGAGCAAAGAAGGTAAGGTCTATTTCACCCCTGTATACCTATGCCATGCCCTC
TTAGGGCCTATTGGCACTGAAATTTTCCTCAGTGACCTCTACCAGCAGCTGTCACTCTCAAAAATTAAAACCAGCCGTAT
GCCCACTCGATTCAAGTATTGA

Upstream 100 bases:

>100_bases
ATTGGCACCGAGCTGTTACTCAAACAATTGCAAGCTGATTTACTGCAGGCAGTAAATTCTCCCACTCAATAAGCCCCAGC
CCTTTTAGGAAAGCGAGCGT

Downstream 100 bases:

>100_bases
CGTTGATCTGCGTGACATCAATGAATATTGAATATTCGACAGTCTTCCATGCTTGAACAACGACTACCAATACTTCTGGA
GTGTTTTCGAGATCTGTAAC

Product: iron(III) dicitrate ABC transporter periplasmic iron(III) dicitrate-binding protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: Ferric-citrate-binding protein [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MARLRLKTMGLLMALAIVGIACTRPTPSTSTGTDCRTITHDLGETEVCGQPQKVVTIGPNLLELLLALEVQPIAHAEYFP
FPTRQFDQPDQQIPYLGKYLTGQPDNVGTAESPSLEAIAKLKPDLILADSIKNKDEYKLLSKIAPTLLFDYSGAGSAWQP
DLKALGQALQKTEKATAVTAESQRQVAKLKQDLQPIVAKDPKVLLLLSEQLSQGVRLETANSACGALLEDVGFQVMVPAA
LSQSQEPSHVISLESLTNLDMDWILIEGFSSQNLDKTSNPEEQQIKAIKTEWSENAIAQSLPPSKEGKVYFTPVYLCHAL
LGPIGTEIFLSDLYQQLSLSKIKTSRMPTRFKY

Sequences:

>Translated_353_residues
MARLRLKTMGLLMALAIVGIACTRPTPSTSTGTDCRTITHDLGETEVCGQPQKVVTIGPNLLELLLALEVQPIAHAEYFP
FPTRQFDQPDQQIPYLGKYLTGQPDNVGTAESPSLEAIAKLKPDLILADSIKNKDEYKLLSKIAPTLLFDYSGAGSAWQP
DLKALGQALQKTEKATAVTAESQRQVAKLKQDLQPIVAKDPKVLLLLSEQLSQGVRLETANSACGALLEDVGFQVMVPAA
LSQSQEPSHVISLESLTNLDMDWILIEGFSSQNLDKTSNPEEQQIKAIKTEWSENAIAQSLPPSKEGKVYFTPVYLCHAL
LGPIGTEIFLSDLYQQLSLSKIKTSRMPTRFKY
>Mature_352_residues
ARLRLKTMGLLMALAIVGIACTRPTPSTSTGTDCRTITHDLGETEVCGQPQKVVTIGPNLLELLLALEVQPIAHAEYFPF
PTRQFDQPDQQIPYLGKYLTGQPDNVGTAESPSLEAIAKLKPDLILADSIKNKDEYKLLSKIAPTLLFDYSGAGSAWQPD
LKALGQALQKTEKATAVTAESQRQVAKLKQDLQPIVAKDPKVLLLLSEQLSQGVRLETANSACGALLEDVGFQVMVPAAL
SQSQEPSHVISLESLTNLDMDWILIEGFSSQNLDKTSNPEEQQIKAIKTEWSENAIAQSLPPSKEGKVYFTPVYLCHALL
GPIGTEIFLSDLYQQLSLSKIKTSRMPTRFKY

Specific function: Part of the ABC transporter complex yfmCDEF involved in citrate-dependent Fe(3+) import. Binds citrate-dependent Fe(3+) and delivers it to the surface of yfmDE (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=254, Percent_Identity=28.3464566929134, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786807, Length=227, Percent_Identity=31.2775330396476, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 38664; Mature: 38533

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLRLKTMGLLMALAIVGIACTRPTPSTSTGTDCRTITHDLGETEVCGQPQKVVTIGPN
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECHH
LLELLLALEVQPIAHAEYFPFPTRQFDQPDQQIPYLGKYLTGQPDNVGTAESPSLEAIAK
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHH
LKPDLILADSIKNKDEYKLLSKIAPTLLFDYSGAGSAWQPDLKALGQALQKTEKATAVTA
CCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ESQRQVAKLKQDLQPIVAKDPKVLLLLSEQLSQGVRLETANSACGALLEDVGFQVMVPAA
HHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCEEEECCHHHHHHHHHCCCEEEECHH
LSQSQEPSHVISLESLTNLDMDWILIEGFSSQNLDKTSNPEEQQIKAIKTEWSENAIAQS
HCCCCCCCCEEEHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
LPPSKEGKVYFTPVYLCHALLGPIGTEIFLSDLYQQLSLSKIKTSRMPTRFKY
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARLRLKTMGLLMALAIVGIACTRPTPSTSTGTDCRTITHDLGETEVCGQPQKVVTIGPN
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECHH
LLELLLALEVQPIAHAEYFPFPTRQFDQPDQQIPYLGKYLTGQPDNVGTAESPSLEAIAK
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHH
LKPDLILADSIKNKDEYKLLSKIAPTLLFDYSGAGSAWQPDLKALGQALQKTEKATAVTA
CCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ESQRQVAKLKQDLQPIVAKDPKVLLLLSEQLSQGVRLETANSACGALLEDVGFQVMVPAA
HHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCEEEECCHHHHHHHHHCCCEEEECHH
LSQSQEPSHVISLESLTNLDMDWILIEGFSSQNLDKTSNPEEQQIKAIKTEWSENAIAQS
HCCCCCCCCEEEHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
LPPSKEGKVYFTPVYLCHALLGPIGTEIFLSDLYQQLSLSKIKTSRMPTRFKY
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]