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The map label for this gene is mnmC [H]

Identifier: 158336439

GI number: 158336439

Start: 3334856

End: 3335752

Strand: Direct

Name: mnmC [H]

Synonym: AM1_3303

Alternate gene names: 158336439

Gene position: 3334856-3335752 (Clockwise)

Preceding gene: 158336438

Following gene: 158336440

Centisome position: 51.28

GC content: 50.61

Gene sequence:

>897_bases
ATGGATAATGCAGAACAGCTTACCCCTCAACTCACAGGTGATGGCTCCTTTACTTTTTATTCCACTGAGTTTGGAGAAAC
TTTCCATAGCCATTATGGAGCGCATCAGGAAGCCATAGGAAAATTTGTCTATCCCACCTTGCTGCCAGAAAAAGCGAAGC
AGGGACCGATCAAGATTCTAGATGTTTGCTATGGCTTGGGGTATAACACTGCTTTGGCTTTGGAGACAATTTGGCAGGTT
AACCCGGATTGCCAGGTTCAGGTTTATGCTCTCGAACTGAATCCCGTGGTACCGAGAGCTGCGATAGCCGAAGGCCGGTC
TTTGACCATCGCAAATGCCGGATTGCAATCGTGGTCACCCCGAGTGCAGACCTATTTAAACACTTTGGCTCACGATCTAG
AGATTCAATCCCCCGATTGTCAGGCACAATTACTTGTAGGTGATGCTCGTCAAACCCTTCAGGTGGTTCTGAAACAGCCG
TTTCTCGCAGATGCGATTTTTCTCGATCCGTTTTCTCCCCCGAACTGTCCGCAGCTTTGGACCGTAGAGTTTTTTCAGCT
GTTAAGGCAATGCCTCCATCCCCAAGGACGCTTAGCGACGTATTCCTGTGCAGCGGCGGTGCGGACGGGATTAATGTCAG
CAGGATTTGCGATCGCAGCCAGTCCCCCTGTGGGTCGGCGCACGACTGGAACCGTTGCCGGGTTCACCCTAACCGATTGC
CTGGCCTTAACGCCTCAAGAGCAAGAGCATCTGCAAACGGTCGCCTCTATTCCTTATCACGATCCTCAGCTGATCGATCC
TGCCTCTACCATTCTGAATCGACGCCAGCAAGCACAAAAACAGTCCTCTTTAGAACCCACCAAAGTTTGGAAAAAACGGT
GGCAAAAGTCCTCCTAG

Upstream 100 bases:

>100_bases
ATAAATTCTGGGAGTAAGGATCAGATACCATCACTATCGCTTAATCTGGTTGAGGTTCGATGAGTAATGAGAAGGGGCTG
TATGGCACCTGTTAGTGGCA

Downstream 100 bases:

>100_bases
GTAACAGTACATAAACAACTAAGACTTTCATGCCTCACCAAAGCCTGAAGTGATAGGCAAGAGAAGGCTCTAGGATTCAG
TGATCCATCATGGCTGGGCT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDNAEQLTPQLTGDGSFTFYSTEFGETFHSHYGAHQEAIGKFVYPTLLPEKAKQGPIKILDVCYGLGYNTALALETIWQV
NPDCQVQVYALELNPVVPRAAIAEGRSLTIANAGLQSWSPRVQTYLNTLAHDLEIQSPDCQAQLLVGDARQTLQVVLKQP
FLADAIFLDPFSPPNCPQLWTVEFFQLLRQCLHPQGRLATYSCAAAVRTGLMSAGFAIAASPPVGRRTTGTVAGFTLTDC
LALTPQEQEHLQTVASIPYHDPQLIDPASTILNRRQQAQKQSSLEPTKVWKKRWQKSS

Sequences:

>Translated_298_residues
MDNAEQLTPQLTGDGSFTFYSTEFGETFHSHYGAHQEAIGKFVYPTLLPEKAKQGPIKILDVCYGLGYNTALALETIWQV
NPDCQVQVYALELNPVVPRAAIAEGRSLTIANAGLQSWSPRVQTYLNTLAHDLEIQSPDCQAQLLVGDARQTLQVVLKQP
FLADAIFLDPFSPPNCPQLWTVEFFQLLRQCLHPQGRLATYSCAAAVRTGLMSAGFAIAASPPVGRRTTGTVAGFTLTDC
LALTPQEQEHLQTVASIPYHDPQLIDPASTILNRRQQAQKQSSLEPTKVWKKRWQKSS
>Mature_298_residues
MDNAEQLTPQLTGDGSFTFYSTEFGETFHSHYGAHQEAIGKFVYPTLLPEKAKQGPIKILDVCYGLGYNTALALETIWQV
NPDCQVQVYALELNPVVPRAAIAEGRSLTIANAGLQSWSPRVQTYLNTLAHDLEIQSPDCQAQLLVGDARQTLQVVLKQP
FLADAIFLDPFSPPNCPQLWTVEFFQLLRQCLHPQGRLATYSCAAAVRTGLMSAGFAIAASPPVGRRTTGTVAGFTLTDC
LALTPQEQEHLQTVASIPYHDPQLIDPASTILNRRQQAQKQSSLEPTKVWKKRWQKSS

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 32881; Mature: 32881

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNAEQLTPQLTGDGSFTFYSTEFGETFHSHYGAHQEAIGKFVYPTLLPEKAKQGPIKIL
CCCHHHCCCEECCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCHHHH
DVCYGLGYNTALALETIWQVNPDCQVQVYALELNPVVPRAAIAEGRSLTIANAGLQSWSP
HHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHCCCEEEEECCCCCCCCH
RVQTYLNTLAHDLEIQSPDCQAQLLVGDARQTLQVVLKQPFLADAIFLDPFSPPNCPQLW
HHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCHHHHEEEECCCCCCCCCCCH
TVEFFQLLRQCLHPQGRLATYSCAAAVRTGLMSAGFAIAASPPVGRRTTGTVAGFTLTDC
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHHH
LALTPQEQEHLQTVASIPYHDPQLIDPASTILNRRQQAQKQSSLEPTKVWKKRWQKSS
HHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MDNAEQLTPQLTGDGSFTFYSTEFGETFHSHYGAHQEAIGKFVYPTLLPEKAKQGPIKIL
CCCHHHCCCEECCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCHHHH
DVCYGLGYNTALALETIWQVNPDCQVQVYALELNPVVPRAAIAEGRSLTIANAGLQSWSP
HHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHCCCEEEEECCCCCCCCH
RVQTYLNTLAHDLEIQSPDCQAQLLVGDARQTLQVVLKQPFLADAIFLDPFSPPNCPQLW
HHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCHHHHEEEECCCCCCCCCCCH
TVEFFQLLRQCLHPQGRLATYSCAAAVRTGLMSAGFAIAASPPVGRRTTGTVAGFTLTDC
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHHH
LALTPQEQEHLQTVASIPYHDPQLIDPASTILNRRQQAQKQSSLEPTKVWKKRWQKSS
HHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]