Click here to switch to the map view.

The map label for this gene is mhpC [C]

Identifier: 158336388

GI number: 158336388

Start: 3285120

End: 3285962

Strand: Reverse

Name: mhpC [C]

Synonym: AM1_3250

Alternate gene names: 158336388

Gene position: 3285962-3285120 (Counterclockwise)

Preceding gene: 158336390

Following gene: 158336387

Centisome position: 50.52

GC content: 45.79

Gene sequence:

>843_bases
ATGAAACCAACTGAGCCCCTACCAGAAGATCGCTATGTCAAAGTTGGGTCTGTCAATACGAGATACTGGCAAATTGGCGA
CTCAGGTAGAGCTGTCATTTTGCTGCATGGAGGTGGCGGTTATATTGAGTTGTGGAAATACAACATCTTTGAATTAGCCA
AGCATCATCGCATTTATGCCTTTGATATGGTCGGTGCAGGTCGCTCTGAAAGACCAAATACCGATTACACATATGATTTT
ATGGCTCAGTTCACCCGAGAGTTTATGAAGGTATTGGATATCCCCAAAGCAGATTTAATTGGAACGTCTGCTGGAGGGGG
TGTGGCGCTGACGTTTGCGCTAAAATTTCCAGCGTTAATCGACCGCCTCGTTTTAGCTGGGAGTGCTGGCTTAGGTCCAG
AAATTAATCTGTTACTTCGGATTACAGCGATTCCGGGGCTAGGTAAGTTATTGAGCTCGCCCACTAAATCAGGTTTAAGA
ATGTTGTGTAAGCAGTCAGTCTATAACTCGAATCTCATTACTGAAGAAATGGTCGATGAGTTCTATCAAATGGCTATCCT
ACCAGGAGCCGCAGCGGCAACGATCAACTTAGGACGCTCCATTTTCAGCATCTGGGGGCAGTTCTCCCAGCCCATTACTG
AGAGACTACAGACCATCACAGCCCCAACCCTCATCATTTGGGGGCAACAAGATCCTATGGTTCCTGTCAGCCATGGTCAG
AATGCTGCTCAAATCATGCCCAATGCTCGATTGGAAATTTTCGAGGAATGTGGTCATTGGAGTTCCATTGAGCATCCCCA
GAAATTCAATCAGATCATTCTAGGATTTCTTTCGTCGTTTTAG

Upstream 100 bases:

>100_bases
AAAAGTTTGTTGATTGGCGTTTTCAGATGCAGCAGACTTTCGGCAATGGTTAAACGTGATAGAAGTGATGTGGTCGCTAC
CCAGTAATGAATCAGCTGAG

Downstream 100 bases:

>100_bases
GTTTTCAAGATGTTGGTTGTTATCTTGATCCTTTCGGAGTGCTTACGATAGTCATCGAACGACGAAAGACGGCTACAAAT
ACCACACTGAAGCCATCTCA

Product: hydrolase, alpha/beta fold family protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKPTEPLPEDRYVKVGSVNTRYWQIGDSGRAVILLHGGGGYIELWKYNIFELAKHHRIYAFDMVGAGRSERPNTDYTYDF
MAQFTREFMKVLDIPKADLIGTSAGGGVALTFALKFPALIDRLVLAGSAGLGPEINLLLRITAIPGLGKLLSSPTKSGLR
MLCKQSVYNSNLITEEMVDEFYQMAILPGAAAATINLGRSIFSIWGQFSQPITERLQTITAPTLIIWGQQDPMVPVSHGQ
NAAQIMPNARLEIFEECGHWSSIEHPQKFNQIILGFLSSF

Sequences:

>Translated_280_residues
MKPTEPLPEDRYVKVGSVNTRYWQIGDSGRAVILLHGGGGYIELWKYNIFELAKHHRIYAFDMVGAGRSERPNTDYTYDF
MAQFTREFMKVLDIPKADLIGTSAGGGVALTFALKFPALIDRLVLAGSAGLGPEINLLLRITAIPGLGKLLSSPTKSGLR
MLCKQSVYNSNLITEEMVDEFYQMAILPGAAAATINLGRSIFSIWGQFSQPITERLQTITAPTLIIWGQQDPMVPVSHGQ
NAAQIMPNARLEIFEECGHWSSIEHPQKFNQIILGFLSSF
>Mature_280_residues
MKPTEPLPEDRYVKVGSVNTRYWQIGDSGRAVILLHGGGGYIELWKYNIFELAKHHRIYAFDMVGAGRSERPNTDYTYDF
MAQFTREFMKVLDIPKADLIGTSAGGGVALTFALKFPALIDRLVLAGSAGLGPEINLLLRITAIPGLGKLLSSPTKSGLR
MLCKQSVYNSNLITEEMVDEFYQMAILPGAAAATINLGRSIFSIWGQFSQPITERLQTITAPTLIIWGQQDPMVPVSHGQ
NAAQIMPNARLEIFEECGHWSSIEHPQKFNQIILGFLSSF

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI221316588, Length=270, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI218777840, Length=267, Percent_Identity=24.3445692883895, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI13376164, Length=267, Percent_Identity=24.3445692883895, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI87081721, Length=286, Percent_Identity=27.972027972028, Blast_Score=111, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32566936, Length=281, Percent_Identity=24.1992882562278, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558492, Length=279, Percent_Identity=24.3727598566308, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31008; Mature: 31008

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTEPLPEDRYVKVGSVNTRYWQIGDSGRAVILLHGGGGYIELWKYNIFELAKHHRIYA
CCCCCCCCCCCEEEECCCCCEEEEECCCCCEEEEEECCCCEEEEEEHHHHHHHHCCEEEE
FDMVGAGRSERPNTDYTYDFMAQFTREFMKVLDIPKADLIGTSAGGGVALTFALKFPALI
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEEEEHHHHHHH
DRLVLAGSAGLGPEINLLLRITAIPGLGKLLSSPTKSGLRMLCKQSVYNSNLITEEMVDE
HHHHHHCCCCCCCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
FYQMAILPGAAAATINLGRSIFSIWGQFSQPITERLQTITAPTLIIWGQQDPMVPVSHGQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCC
NAAQIMPNARLEIFEECGHWSSIEHPQKFNQIILGFLSSF
CHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPTEPLPEDRYVKVGSVNTRYWQIGDSGRAVILLHGGGGYIELWKYNIFELAKHHRIYA
CCCCCCCCCCCEEEECCCCCEEEEECCCCCEEEEEECCCCEEEEEEHHHHHHHHCCEEEE
FDMVGAGRSERPNTDYTYDFMAQFTREFMKVLDIPKADLIGTSAGGGVALTFALKFPALI
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEEEEHHHHHHH
DRLVLAGSAGLGPEINLLLRITAIPGLGKLLSSPTKSGLRMLCKQSVYNSNLITEEMVDE
HHHHHHCCCCCCCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
FYQMAILPGAAAATINLGRSIFSIWGQFSQPITERLQTITAPTLIIWGQQDPMVPVSHGQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCC
NAAQIMPNARLEIFEECGHWSSIEHPQKFNQIILGFLSSF
CHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]