Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 158336382

GI number: 158336382

Start: 3280071

End: 3281456

Strand: Direct

Name: gdhA [H]

Synonym: AM1_3244

Alternate gene names: 158336382

Gene position: 3280071-3281456 (Clockwise)

Preceding gene: 158336380

Following gene: 158336383

Centisome position: 50.43

GC content: 50.07

Gene sequence:

>1386_bases
ATGTTTGTTGAATCTCGACTGAGATCGCACGCCCAAACCCAAAGTCGAGAGTACGCTAGAATCAGCCCTTCACAGCAGGA
TAGTCTCGTGGGTACGGATGATCAACTCTTTAGCGATGCCACTCACCGCCTAACCACTGCCTTAGAGCATGTGGATTTAC
CCGAAGATGTTTTGGAAACCTTGAAATATCCTAAGGCCCAACTCGGAGTCTCCATTCCGGTGCGTATGGATAATGGCGAA
CTGAAAATCTTTCAAGGCTATCGTGTCCGCTATGACGACACTCGCGGTCCGGCTAAGGGTGGGGTTAGGTATCATCCCGG
CGTCTCCATTGATGAAGTCCAGTCCTTGGCATTTTGGATGACCTTTAAATGTGCTGCCCTAAACTTGCCCTTTGGCGGTG
GCAAAGGCGGGATTACGGTCGACCCTAAATCCCTCTCCCGGATGGAGCTAGAGCGTTTAAGCCGAGGCTATATTGATGCC
ATTGCTGATTTTATTGGCCCGGATGTGGATATTCTGGCTCCCGATGTCTATACCAACCCGATGATTATGGGCTGGATGAT
GGATCAGTACAGCATTATCAAACGCCAAATTTGCCGAGGAGTGGTCACGGGAAAACCCCTAGCAATTGGGGGCAGTGTAG
GCAGAAACACAGCCACGGGGATGGGAGCTTTTTTCGTGATTGAAGCGATGGCACCAAAGTTGGAGCTGATTCCTGAGCAA
ACCACAGTGGCGGTGCAAGGGTTTGGTAATGCTGGAGCGGTTGTTGCCGAGCTGCTAAATAAAGTGGGCTATAAAGTCGT
TGCCGTTAGCGATTCTCAGGGCGGTATTTATGCCCCGCAAGGTTTGGATATTGCCAGTATCCGCAAGCATAAAGACGCCA
GCCGCTCCATGAAGGCGGTCTATTGTGATGGCAGTGTCTGCAGCATTATTGAGCATGACACGATCACGAATGAAGAGCTG
TTGGCCTTAGACGTGGATGTGTTGATTCCTGCTGCTTTGGAGAATCAAATCACGGCTGATAATGCCCAGCAGATCAAGGC
GAAATATATTTTCGAGGTGGCTAATGGTCCTGTCACTTCAGCCGCAGATGCAATTTTAGTAGAGTCTGGCACGACGGTCT
TCCCGGATATTTTGGTAAATGCGGGGGGCGTCACGGTGAGCTATTTTGAATGGGTGCAAAATCGGAGTGGTCTCTACTGG
ACGAAGGCAGAGGTGCAGAAGCAGCTTCAGCAAAAGATGGTGGAGGAGACGGAGACAATTTGGCAAATTGCTCAAACTAA
AACAATCTCTGTAAGGACTGCGGCCTATGTGCATGCCCTCAACCGAATTGGTGAAGCTGTAACGGCTAAAGGGACAAGGG
ACTACTATGCGAATCACCCATCATGA

Upstream 100 bases:

>100_bases
ATTGCATTGTTCCCCCTTCCTTGGGTTTTAGAGAATAATAGCGTGATTTGTTCTTTTTGCCGGTCCTTGGCTTTGCAGCG
ATTAATGGTTGCGTTTCGTT

Downstream 100 bases:

>100_bases
TCTGAATTTGTGTCCTGTCGCTATTGCTTTAATTGGTACGGGAATTTCCACTTTTTTGAGTCAAGGTCAGTGTCTACAGC
AACAGAACGGCTAAAAATTA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MFVESRLRSHAQTQSREYARISPSQQDSLVGTDDQLFSDATHRLTTALEHVDLPEDVLETLKYPKAQLGVSIPVRMDNGE
LKIFQGYRVRYDDTRGPAKGGVRYHPGVSIDEVQSLAFWMTFKCAALNLPFGGGKGGITVDPKSLSRMELERLSRGYIDA
IADFIGPDVDILAPDVYTNPMIMGWMMDQYSIIKRQICRGVVTGKPLAIGGSVGRNTATGMGAFFVIEAMAPKLELIPEQ
TTVAVQGFGNAGAVVAELLNKVGYKVVAVSDSQGGIYAPQGLDIASIRKHKDASRSMKAVYCDGSVCSIIEHDTITNEEL
LALDVDVLIPAALENQITADNAQQIKAKYIFEVANGPVTSAADAILVESGTTVFPDILVNAGGVTVSYFEWVQNRSGLYW
TKAEVQKQLQQKMVEETETIWQIAQTKTISVRTAAYVHALNRIGEAVTAKGTRDYYANHPS

Sequences:

>Translated_461_residues
MFVESRLRSHAQTQSREYARISPSQQDSLVGTDDQLFSDATHRLTTALEHVDLPEDVLETLKYPKAQLGVSIPVRMDNGE
LKIFQGYRVRYDDTRGPAKGGVRYHPGVSIDEVQSLAFWMTFKCAALNLPFGGGKGGITVDPKSLSRMELERLSRGYIDA
IADFIGPDVDILAPDVYTNPMIMGWMMDQYSIIKRQICRGVVTGKPLAIGGSVGRNTATGMGAFFVIEAMAPKLELIPEQ
TTVAVQGFGNAGAVVAELLNKVGYKVVAVSDSQGGIYAPQGLDIASIRKHKDASRSMKAVYCDGSVCSIIEHDTITNEEL
LALDVDVLIPAALENQITADNAQQIKAKYIFEVANGPVTSAADAILVESGTTVFPDILVNAGGVTVSYFEWVQNRSGLYW
TKAEVQKQLQQKMVEETETIWQIAQTKTISVRTAAYVHALNRIGEAVTAKGTRDYYANHPS
>Mature_461_residues
MFVESRLRSHAQTQSREYARISPSQQDSLVGTDDQLFSDATHRLTTALEHVDLPEDVLETLKYPKAQLGVSIPVRMDNGE
LKIFQGYRVRYDDTRGPAKGGVRYHPGVSIDEVQSLAFWMTFKCAALNLPFGGGKGGITVDPKSLSRMELERLSRGYIDA
IADFIGPDVDILAPDVYTNPMIMGWMMDQYSIIKRQICRGVVTGKPLAIGGSVGRNTATGMGAFFVIEAMAPKLELIPEQ
TTVAVQGFGNAGAVVAELLNKVGYKVVAVSDSQGGIYAPQGLDIASIRKHKDASRSMKAVYCDGSVCSIIEHDTITNEEL
LALDVDVLIPAALENQITADNAQQIKAKYIFEVANGPVTSAADAILVESGTTVFPDILVNAGGVTVSYFEWVQNRSGLYW
TKAEVQKQLQQKMVEETETIWQIAQTKTISVRTAAYVHALNRIGEAVTAKGTRDYYANHPS

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:
GO:0005488: Glutamate dehydrogenase
GO:0006520: Glutamate dehydrogenase
GO:0016491: Glutamate dehydrogenase
GO:0016639: Glutamate dehydrogenase
GO:0055114: Glutamate dehydrogenase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=398, Percent_Identity=38.4422110552764, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI4885281, Length=398, Percent_Identity=38.1909547738693, Blast_Score=252, Evalue=5e-67,
Organism=Escherichia coli, GI1788059, Length=374, Percent_Identity=30.2139037433155, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17544676, Length=357, Percent_Identity=37.2549019607843, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6319256, Length=435, Percent_Identity=29.8850574712644, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324951, Length=415, Percent_Identity=30.1204819277108, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45549226, Length=375, Percent_Identity=35.4666666666667, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI45553475, Length=338, Percent_Identity=37.5739644970414, Blast_Score=238, Evalue=9e-63,
Organism=Drosophila melanogaster, GI24649283, Length=351, Percent_Identity=37.3219373219373, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24649460, Length=325, Percent_Identity=35.3846153846154, Blast_Score=198, Evalue=6e-51,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 50302; Mature: 50302

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVESRLRSHAQTQSREYARISPSQQDSLVGTDDQLFSDATHRLTTALEHVDLPEDVLET
CCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LKYPKAQLGVSIPVRMDNGELKIFQGYRVRYDDTRGPAKGGVRYHPGVSIDEVQSLAFWM
HCCCHHHCCEEEEEEECCCCEEEEECEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHH
TFKCAALNLPFGGGKGGITVDPKSLSRMELERLSRGYIDAIADFIGPDVDILAPDVYTNP
EEEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
MIMGWMMDQYSIIKRQICRGVVTGKPLAIGGSVGRNTATGMGAFFVIEAMAPKLELIPEQ
EEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCEECCCC
TTVAVQGFGNAGAVVAELLNKVGYKVVAVSDSQGGIYAPQGLDIASIRKHKDASRSMKAV
EEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCEECCCCCCHHHHHHHCCCCCCCEEE
YCDGSVCSIIEHDTITNEELLALDVDVLIPAALENQITADNAQQIKAKYIFEVANGPVTS
EECCCEEEHEECCCCCCCCEEEEEHHHHCCHHHCCCCCCCCHHHHHHEEEEEECCCCCCC
AADAILVESGTTVFPDILVNAGGVTVSYFEWVQNRSGLYWTKAEVQKQLQQKMVEETETI
CCCEEEEECCCEECCHHHCCCCCCEEHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHH
WQIAQTKTISVRTAAYVHALNRIGEAVTAKGTRDYYANHPS
HHHHHCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MFVESRLRSHAQTQSREYARISPSQQDSLVGTDDQLFSDATHRLTTALEHVDLPEDVLET
CCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LKYPKAQLGVSIPVRMDNGELKIFQGYRVRYDDTRGPAKGGVRYHPGVSIDEVQSLAFWM
HCCCHHHCCEEEEEEECCCCEEEEECEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHH
TFKCAALNLPFGGGKGGITVDPKSLSRMELERLSRGYIDAIADFIGPDVDILAPDVYTNP
EEEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
MIMGWMMDQYSIIKRQICRGVVTGKPLAIGGSVGRNTATGMGAFFVIEAMAPKLELIPEQ
EEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCEECCCC
TTVAVQGFGNAGAVVAELLNKVGYKVVAVSDSQGGIYAPQGLDIASIRKHKDASRSMKAV
EEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCEECCCCCCHHHHHHHCCCCCCCEEE
YCDGSVCSIIEHDTITNEELLALDVDVLIPAALENQITADNAQQIKAKYIFEVANGPVTS
EECCCEEEHEECCCCCCCCEEEEEHHHHCCHHHCCCCCCCCHHHHHHEEEEEECCCCCCC
AADAILVESGTTVFPDILVNAGGVTVSYFEWVQNRSGLYWTKAEVQKQLQQKMVEETETI
CCCEEEEECCCEECCHHHCCCCCCEEHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHH
WQIAQTKTISVRTAAYVHALNRIGEAVTAKGTRDYYANHPS
HHHHHCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7787182; 8590279; 8905231 [H]