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The map label for this gene is ilvD

Identifier: 158336281

GI number: 158336281

Start: 3169034

End: 3170719

Strand: Direct

Name: ilvD

Synonym: AM1_3143

Alternate gene names: 158336281

Gene position: 3169034-3170719 (Clockwise)

Preceding gene: 158336277

Following gene: 158336284

Centisome position: 48.73

GC content: 51.01

Gene sequence:

>1686_bases
ATGTCTGACAATCGAAATAGCCAAGTCGTCACTCAAGGAGTTCAACGAGCCCCCAACCGGGCCATGCTAAGGGCTGTGGG
CTTTGGAGATGATGATTTTACCAAGCCGATTGTTGGGTTAGCGAATGGTTTTAGTACGATTACCCCTTGTAATATGGGCA
TAGACTCTCTGGCTACACGGGCAGAGGCCAGTATCAGAACGGCTGGAGCCATGCCGCAAAAGTTTGGCACCATCACTATC
AGTGACGGCATTTCCATGGGCACGGAAGGGATGAAATATTCCTTGGTGTCCCGGGAAGTGATTGCAGACTCCATCGAGAC
GGCCTGTATGGGCCAAAGTATGGATGGAGTACTCGCCATTGGCGGCTGTGATAAAAATATGCCGGGGGCTATGCTCGCCA
TGGCTCGGATGAATATTCCGGCCATCTTTGTTTACGGCGGCACGATCAAACCCGGACATCTAAATGGTGAAGACTTAACC
GTGGTTAGTGCCTTTGAAGCTGTGGGTCAGCATAGTGCTGGACGGATTAGTGAAGCCGAGTTAACGGCAGTGGAGAAACA
TGCTTGTCCGGGTGCTGGATCCTGTGGCGGTATGTATACGGCCAACACCATGTCTTCTGCGTTTGAGGCAATGGGCATGA
GCCTGATGTATTCTTCCACCATGGCAGCGGAAGATGAAGAAAAAGCCGTCAGTGCAGAACAGTCTGCCGCTGTTTTAGTG
GAAGCGATCCACAAGCAAATTCTGCCCCGCGATATCTTGACCCGCAAAGCCTTTGAAAATGCCATTGCTGTGATTATGGC
TGTAGGCGGATCCACCAATGCCGTTCTCCACCTGTTGGCCATTTCCCGCGCGGCTGGAGACTCCCTGACCCTAGATGACT
TCGAAACCATCCGGGCTCAAGTTCCAGTGATCTGCGATCTCAAACCATCAGGTCGCTATGTAGCCACAGATCTCCATAAA
GCAGGTGGAATCCCATTGGTGATGAAAATGCTATTGGAACATGGTCTTCTCCATGGTGATGCTCTGACCATTACGGGTAA
AACCATTGCTGAGCAGTTGGCTGATGTCCCATCAGAGCCTTCCCCCGATCAGGATGTAATTCGGCCTTGGGACAATCCCA
TGTATAAACAAGGCCACCTCGCCATCCTCCGGGGTAACTTGGCCACGGAAGGAGCGGTTGCCAAAATCACAGGCATTAAA
AATCCCCAAATTACGGGTCCAGCTCGGGTATTTGAATCGGAAGAGGCTTGCTTGGAGGCGATTTTGGCAGGCAAGATTCA
GCCTAATGATGTGATTGTGGTGCGGTATGAAGGCCCGAAGGGTGGGCCGGGAATGCGAGAGATGTTAGCTCCCACGTCCG
CGATTATTGGGGCAGGTTTGGGTGATTCAGTTGGATTGATTACGGATGGTCGCTTTTCTGGTGGAACTTACGGCATGGTG
GTTGGGCATGTGGCCCCAGAAGCTGCGGTGGGTGGTACGATTGCCCTTGTGCAGGAAGGCGATCAGATTACAATTGATGC
CCATGCTCGAAAGTTGGAGCTGCATGTGTCTGATCAGGAGTTAAAAGAGCGGAAGGAGAAGTGGGAGCAACCAAAACCTC
TCTATAACAAAGGGGTTTTGGCAAAGTATGCCAAGTTAGTTTCTTCTAGTAGTGTTGGAGCTGTTACTGATTTAGATCTA
TTTTGA

Upstream 100 bases:

>100_bases
ACAGTTGAGTAAGGTCATTGGTTCCAACCTGAGTTCTGCTACTCACGGGTCGTAATCTTAGCCTTCTCTTGCTTTTGCGT
CGTGAACAGAAAGAAGTCGT

Downstream 100 bases:

>100_bases
TCATCGTATTCTCTATCACTTATGCCTCAGCCAACGTGAATTAAGCTTCTTGATCTTAGATGATAAAAGATTTAGCTTTT
TTCTTCATTAGGAGGAGCAC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MSDNRNSQVVTQGVQRAPNRAMLRAVGFGDDDFTKPIVGLANGFSTITPCNMGIDSLATRAEASIRTAGAMPQKFGTITI
SDGISMGTEGMKYSLVSREVIADSIETACMGQSMDGVLAIGGCDKNMPGAMLAMARMNIPAIFVYGGTIKPGHLNGEDLT
VVSAFEAVGQHSAGRISEAELTAVEKHACPGAGSCGGMYTANTMSSAFEAMGMSLMYSSTMAAEDEEKAVSAEQSAAVLV
EAIHKQILPRDILTRKAFENAIAVIMAVGGSTNAVLHLLAISRAAGDSLTLDDFETIRAQVPVICDLKPSGRYVATDLHK
AGGIPLVMKMLLEHGLLHGDALTITGKTIAEQLADVPSEPSPDQDVIRPWDNPMYKQGHLAILRGNLATEGAVAKITGIK
NPQITGPARVFESEEACLEAILAGKIQPNDVIVVRYEGPKGGPGMREMLAPTSAIIGAGLGDSVGLITDGRFSGGTYGMV
VGHVAPEAAVGGTIALVQEGDQITIDAHARKLELHVSDQELKERKEKWEQPKPLYNKGVLAKYAKLVSSSSVGAVTDLDL
F

Sequences:

>Translated_561_residues
MSDNRNSQVVTQGVQRAPNRAMLRAVGFGDDDFTKPIVGLANGFSTITPCNMGIDSLATRAEASIRTAGAMPQKFGTITI
SDGISMGTEGMKYSLVSREVIADSIETACMGQSMDGVLAIGGCDKNMPGAMLAMARMNIPAIFVYGGTIKPGHLNGEDLT
VVSAFEAVGQHSAGRISEAELTAVEKHACPGAGSCGGMYTANTMSSAFEAMGMSLMYSSTMAAEDEEKAVSAEQSAAVLV
EAIHKQILPRDILTRKAFENAIAVIMAVGGSTNAVLHLLAISRAAGDSLTLDDFETIRAQVPVICDLKPSGRYVATDLHK
AGGIPLVMKMLLEHGLLHGDALTITGKTIAEQLADVPSEPSPDQDVIRPWDNPMYKQGHLAILRGNLATEGAVAKITGIK
NPQITGPARVFESEEACLEAILAGKIQPNDVIVVRYEGPKGGPGMREMLAPTSAIIGAGLGDSVGLITDGRFSGGTYGMV
VGHVAPEAAVGGTIALVQEGDQITIDAHARKLELHVSDQELKERKEKWEQPKPLYNKGVLAKYAKLVSSSSVGAVTDLDL
F
>Mature_560_residues
SDNRNSQVVTQGVQRAPNRAMLRAVGFGDDDFTKPIVGLANGFSTITPCNMGIDSLATRAEASIRTAGAMPQKFGTITIS
DGISMGTEGMKYSLVSREVIADSIETACMGQSMDGVLAIGGCDKNMPGAMLAMARMNIPAIFVYGGTIKPGHLNGEDLTV
VSAFEAVGQHSAGRISEAELTAVEKHACPGAGSCGGMYTANTMSSAFEAMGMSLMYSSTMAAEDEEKAVSAEQSAAVLVE
AIHKQILPRDILTRKAFENAIAVIMAVGGSTNAVLHLLAISRAAGDSLTLDDFETIRAQVPVICDLKPSGRYVATDLHKA
GGIPLVMKMLLEHGLLHGDALTITGKTIAEQLADVPSEPSPDQDVIRPWDNPMYKQGHLAILRGNLATEGAVAKITGIKN
PQITGPARVFESEEACLEAILAGKIQPNDVIVVRYEGPKGGPGMREMLAPTSAIIGAGLGDSVGLITDGRFSGGTYGMVV
GHVAPEAAVGGTIALVQEGDQITIDAHARKLELHVSDQELKERKEKWEQPKPLYNKGVLAKYAKLVSSSSVGAVTDLDLF

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:
GO:0003824: Dihydroxy-acid dehydratase
GO:0004160: Dihydroxy-acid dehydratase
GO:0008152: Dihydroxy-acid dehydratase
GO:0008652: Dihydroxy-acid dehydratase
GO:0009082: Dihydroxy-acid dehydratase
GO:0016829: Dihydroxy-acid dehydratase
GO:0046872: Dihydroxy-acid dehydratase
GO:0051536: Dihydroxy-acid dehydratase
GO:0051539: Dihydroxy-acid dehydratase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=605, Percent_Identity=38.1818181818182, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1788157, Length=472, Percent_Identity=32.2033898305085, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786464, Length=459, Percent_Identity=35.2941176470588, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI2367371, Length=501, Percent_Identity=32.5349301397206, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322476, Length=562, Percent_Identity=49.644128113879, Blast_Score=553, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_ACAM1 (B0CEN4)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517455.1
- ProteinModelPortal:   B0CEN4
- SMR:   B0CEN4
- GeneID:   5681951
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3143
- HOGENOM:   HBG671001
- OMA:   NMPGAMI
- ProtClustDB:   PRK00911
- BioCyc:   AMAR329726:AM1_3143-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 58905; Mature: 58774

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNRNSQVVTQGVQRAPNRAMLRAVGFGDDDFTKPIVGLANGFSTITPCNMGIDSLATR
CCCCCCHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AEASIRTAGAMPQKFGTITISDGISMGTEGMKYSLVSREVIADSIETACMGQSMDGVLAI
HHHHHHHHCCCCHHCCEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGAMLAMARMNIPAIFVYGGTIKPGHLNGEDLTVVSAFEAVGQHSAGRISEAE
ECCCCCCCHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHH
LTAVEKHACPGAGSCGGMYTANTMSSAFEAMGMSLMYSSTMAAEDEEKAVSAEQSAAVLV
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EAIHKQILPRDILTRKAFENAIAVIMAVGGSTNAVLHLLAISRAAGDSLTLDDFETIRAQ
HHHHHHHCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
VPVICDLKPSGRYVATDLHKAGGIPLVMKMLLEHGLLHGDALTITGKTIAEQLADVPSEP
CCEEEECCCCCCEEEEEHHHCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCCC
SPDQDVIRPWDNPMYKQGHLAILRGNLATEGAVAKITGIKNPQITGPARVFESEEACLEA
CCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHH
ILAGKIQPNDVIVVRYEGPKGGPGMREMLAPTSAIIGAGLGDSVGLITDGRFSGGTYGMV
HHHCCCCCCCEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCCCCEEEECCCCCCCEEEEE
VGHVAPEAAVGGTIALVQEGDQITIDAHARKLELHVSDQELKERKEKWEQPKPLYNKGVL
EECCCCCHHCCCEEEEEECCCEEEEECCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCHH
AKYAKLVSSSSVGAVTDLDLF
HHHHHHHCCCCCCCEECCCCC
>Mature Secondary Structure 
SDNRNSQVVTQGVQRAPNRAMLRAVGFGDDDFTKPIVGLANGFSTITPCNMGIDSLATR
CCCCCHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AEASIRTAGAMPQKFGTITISDGISMGTEGMKYSLVSREVIADSIETACMGQSMDGVLAI
HHHHHHHHCCCCHHCCEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGAMLAMARMNIPAIFVYGGTIKPGHLNGEDLTVVSAFEAVGQHSAGRISEAE
ECCCCCCCHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHH
LTAVEKHACPGAGSCGGMYTANTMSSAFEAMGMSLMYSSTMAAEDEEKAVSAEQSAAVLV
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EAIHKQILPRDILTRKAFENAIAVIMAVGGSTNAVLHLLAISRAAGDSLTLDDFETIRAQ
HHHHHHHCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
VPVICDLKPSGRYVATDLHKAGGIPLVMKMLLEHGLLHGDALTITGKTIAEQLADVPSEP
CCEEEECCCCCCEEEEEHHHCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCCC
SPDQDVIRPWDNPMYKQGHLAILRGNLATEGAVAKITGIKNPQITGPARVFESEEACLEA
CCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHH
ILAGKIQPNDVIVVRYEGPKGGPGMREMLAPTSAIIGAGLGDSVGLITDGRFSGGTYGMV
HHHCCCCCCCEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCCCCEEEECCCCCCCEEEEE
VGHVAPEAAVGGTIALVQEGDQITIDAHARKLELHVSDQELKERKEKWEQPKPLYNKGVL
EECCCCCHHCCCEEEEEECCCEEEEECCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCHH
AKYAKLVSSSSVGAVTDLDLF
HHHHHHHCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA