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The map label for this gene is pilT [H]

Identifier: 158336258

GI number: 158336258

Start: 3148973

End: 3150328

Strand: Direct

Name: pilT [H]

Synonym: AM1_3120

Alternate gene names: 158336258

Gene position: 3148973-3150328 (Clockwise)

Preceding gene: 158336254

Following gene: 158336259

Centisome position: 48.42

GC content: 48.67

Gene sequence:

>1356_bases
ATGACAGATTACCAGCGTCCACCCTTTCCACCCCCACCCGCACCTTTACCAGCGGTTCCCCCTCCTCCTCATCGTGATCA
ACGCTCGGAAGGAGACGCGATGGAAACCGAAATGATTGCCACCCCTACGAAAGTGGTGCCGACAAAAGTGGTGAGCGCAC
CCCCCCCTCAAGCAGCTGAGGAACCCACTGCTTTTCCTCAAACTCAACCCTATGCTGGATATGGGCAAACTGAGGTTGAT
AATGGTGAGCCGACTCAATCGACATTACAAATGCGAGGTCGACCTCCGGGAACTGGCGTGACCTTGGAACAACTGGTTCG
TGAAGCCTTTGAGAATAACTTTTCGGATATTCACTTAGGCGTGGGTGAAGTCCCTCGCTTTCGAGATCGCGGTCGCCTAG
AAATGTCTCGTCATCCTGTTACAGATGACACGACCTTCGAATATTGGTTGGAAGAAATTTTAACGCCTGAAGAAATCCAG
CGGTTTCATGCTGAGCAGGAATACGATGGTGCCACTCAATACGATGGCATGGCTCGGGTTCGAATCAATATTTTTGTCAG
TCTAAAAGGGCCATCGATGGTCATGCGATTGATTCCTTTAAAGATTTTGACCTTAGAAGATCTCAATCTCCCCCTCGTGT
TTCAGGATTTGTGTCACTACCACAAGGGTTTAATTCTGGTAACGGGACCCACCGGATCGGGTAAATCCACGACGATGGCA
GCCATGGTTGATTACATCAACACAGAAATGCCGAAACATATTATCTCCATTGAAGATCCGATTGAATTCGTCCATCAAAG
TCGCAAAGCGATGATTCGCCAGCGGGAAGTGGGAATTCATACCAAGAAATTTGATAACGCCCTAAAAGCGTCCTTGCGTG
AGGACCCGGATGTCATTCTGATTGGGGAAATGCGAGATCGTGAAACGGTGAATACAGCCTTAAAAGCAGCCCAGACCGGT
CACTTGGTGTTTGGAACCCTGCACACCAACAGTGCAGTGAAAACGATTGAGAGAATTCTCAACCTGTATAAGCCAGAAGA
ACAAGAACCCATGCGGGTCCAAGTGGCTGAATCCTTGGTGGCGGTGATTGCCCAATCTCTCGTACGAACCACCGATGGCA
AACGAGCGGCGATTCATGAAATCATGATTAACACCGATGCCATTAAGGACTACATCCTACGCGGTGATGTAGAAGAAGTC
GAGGCGATGATTCCACAATGTAGTTATGACGGCATGATTACGATGAATCAGTGTCTGCATAATCTCTACGAGGAAGGCCG
CATTGATGAAGAAACGGCTCTGGAAATGTCTCCCAAGCCGAATGAAATGGCTCAAATCCTAAGAGGACGTATCTAG

Upstream 100 bases:

>100_bases
CATCAGTTGCAAATTAAGTAAGATTGCCCCTTTTGGCTTCATCGTTGAGCACTGAAGAGTATTCAAACCAGTATGTCCAT
CGCTTGAGATAAGTATTGCT

Downstream 100 bases:

>100_bases
CCAGCATTGGAGAGGTTTGTTATAAGGTATCTGATAGGTCTCTGGTCTAACATGGACTGCTTGTGACTACTTTGCGATCG
CAACCTCTCCCCTTTCTATA

Product: type IV pili twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTDYQRPPFPPPPAPLPAVPPPPHRDQRSEGDAMETEMIATPTKVVPTKVVSAPPPQAAEEPTAFPQTQPYAGYGQTEVD
NGEPTQSTLQMRGRPPGTGVTLEQLVREAFENNFSDIHLGVGEVPRFRDRGRLEMSRHPVTDDTTFEYWLEEILTPEEIQ
RFHAEQEYDGATQYDGMARVRINIFVSLKGPSMVMRLIPLKILTLEDLNLPLVFQDLCHYHKGLILVTGPTGSGKSTTMA
AMVDYINTEMPKHIISIEDPIEFVHQSRKAMIRQREVGIHTKKFDNALKASLREDPDVILIGEMRDRETVNTALKAAQTG
HLVFGTLHTNSAVKTIERILNLYKPEEQEPMRVQVAESLVAVIAQSLVRTTDGKRAAIHEIMINTDAIKDYILRGDVEEV
EAMIPQCSYDGMITMNQCLHNLYEEGRIDEETALEMSPKPNEMAQILRGRI

Sequences:

>Translated_451_residues
MTDYQRPPFPPPPAPLPAVPPPPHRDQRSEGDAMETEMIATPTKVVPTKVVSAPPPQAAEEPTAFPQTQPYAGYGQTEVD
NGEPTQSTLQMRGRPPGTGVTLEQLVREAFENNFSDIHLGVGEVPRFRDRGRLEMSRHPVTDDTTFEYWLEEILTPEEIQ
RFHAEQEYDGATQYDGMARVRINIFVSLKGPSMVMRLIPLKILTLEDLNLPLVFQDLCHYHKGLILVTGPTGSGKSTTMA
AMVDYINTEMPKHIISIEDPIEFVHQSRKAMIRQREVGIHTKKFDNALKASLREDPDVILIGEMRDRETVNTALKAAQTG
HLVFGTLHTNSAVKTIERILNLYKPEEQEPMRVQVAESLVAVIAQSLVRTTDGKRAAIHEIMINTDAIKDYILRGDVEEV
EAMIPQCSYDGMITMNQCLHNLYEEGRIDEETALEMSPKPNEMAQILRGRI
>Mature_450_residues
TDYQRPPFPPPPAPLPAVPPPPHRDQRSEGDAMETEMIATPTKVVPTKVVSAPPPQAAEEPTAFPQTQPYAGYGQTEVDN
GEPTQSTLQMRGRPPGTGVTLEQLVREAFENNFSDIHLGVGEVPRFRDRGRLEMSRHPVTDDTTFEYWLEEILTPEEIQR
FHAEQEYDGATQYDGMARVRINIFVSLKGPSMVMRLIPLKILTLEDLNLPLVFQDLCHYHKGLILVTGPTGSGKSTTMAA
MVDYINTEMPKHIISIEDPIEFVHQSRKAMIRQREVGIHTKKFDNALKASLREDPDVILIGEMRDRETVNTALKAAQTGH
LVFGTLHTNSAVKTIERILNLYKPEEQEPMRVQVAESLVAVIAQSLVRTTDGKRAAIHEIMINTDAIKDYILRGDVEEVE
AMIPQCSYDGMITMNQCLHNLYEEGRIDEETALEMSPKPNEMAQILRGRI

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=40.1215805471125, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1786296, Length=280, Percent_Identity=35.3571428571429, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789723, Length=197, Percent_Identity=38.5786802030457, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 50681; Mature: 50549

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYQRPPFPPPPAPLPAVPPPPHRDQRSEGDAMETEMIATPTKVVPTKVVSAPPPQAAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHCCHHHCCCCCCCCCC
EPTAFPQTQPYAGYGQTEVDNGEPTQSTLQMRGRPPGTGVTLEQLVREAFENNFSDIHLG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
VGEVPRFRDRGRLEMSRHPVTDDTTFEYWLEEILTPEEIQRFHAEQEYDGATQYDGMARV
CCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEE
RINIFVSLKGPSMVMRLIPLKILTLEDLNLPLVFQDLCHYHKGLILVTGPTGSGKSTTMA
EEEEEEEECCHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHH
AMVDYINTEMPKHIISIEDPIEFVHQSRKAMIRQREVGIHTKKFDNALKASLREDPDVIL
HHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEE
IGEMRDRETVNTALKAAQTGHLVFGTLHTNSAVKTIERILNLYKPEEQEPMRVQVAESLV
ECCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AVIAQSLVRTTDGKRAAIHEIMINTDAIKDYILRGDVEEVEAMIPQCSYDGMITMNQCLH
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEHHHHHH
NLYEEGRIDEETALEMSPKPNEMAQILRGRI
HHHHHCCCCHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TDYQRPPFPPPPAPLPAVPPPPHRDQRSEGDAMETEMIATPTKVVPTKVVSAPPPQAAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHCCHHHCCCCCCCCCC
EPTAFPQTQPYAGYGQTEVDNGEPTQSTLQMRGRPPGTGVTLEQLVREAFENNFSDIHLG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
VGEVPRFRDRGRLEMSRHPVTDDTTFEYWLEEILTPEEIQRFHAEQEYDGATQYDGMARV
CCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEE
RINIFVSLKGPSMVMRLIPLKILTLEDLNLPLVFQDLCHYHKGLILVTGPTGSGKSTTMA
EEEEEEEECCHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHH
AMVDYINTEMPKHIISIEDPIEFVHQSRKAMIRQREVGIHTKKFDNALKASLREDPDVIL
HHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEE
IGEMRDRETVNTALKAAQTGHLVFGTLHTNSAVKTIERILNLYKPEEQEPMRVQVAESLV
ECCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AVIAQSLVRTTDGKRAAIHEIMINTDAIKDYILRGDVEEVEAMIPQCSYDGMITMNQCLH
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEHHHHHH
NLYEEGRIDEETALEMSPKPNEMAQILRGRI
HHHHHCCCCHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]