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The map label for this gene is dam [H]

Identifier: 158336217

GI number: 158336217

Start: 3108009

End: 3108842

Strand: Reverse

Name: dam [H]

Synonym: AM1_3079

Alternate gene names: 158336217

Gene position: 3108842-3108009 (Counterclockwise)

Preceding gene: 158336222

Following gene: 158336216

Centisome position: 47.8

GC content: 47.96

Gene sequence:

>834_bases
ATGACTAGTCCATTGCCGACTCAGGCGCGAGTTCCTGCCCGTCCGTTTCTGAAATGGGCAGGGGGCAAAGGTCGATTAAT
CACTCAATATGGTCCGCATTTCCCTCAGAACTTTGGTGCTTACTATGAGCCGTTCTTAGGCGGTGGTGCTATCTTTTTCC
ATTTACAGCCGGAGCAGGCAGTTCTTTCTGATGTGAATCCTGAACTGGTCAATGTTTATACCTGTGTTCGCGATCGGATA
GATGACGTCTTGAAATATCTGGAGACGCATGCCTGCCAGCATGGTCATGACTACTACTACCAAATTCGAGCTCTCAATCC
ATCCTTACCCGCTGAACGTGCCGCTCGATTTATTTATCTCAATCGCACCTGTTTCAATGGTCTGTATCGAGAAAACTCCA
AAGGACAGTTCAATGTGCCTATGGGCCGATACAAGAAACCCAAAATTTGTGATGTAGTGCTCCTGAAAGCGGTTTCTGAA
GTTCTCCAAGCCGTTCAGATCCACCAGCAGCCGTTTGAAACGATTTTGGAGGTGGCCCAATCAGCCCAGGATTTTGTCTA
TTTCGATCCGCCCTATCATCCCATCAGCGCTACAAGCGATTTTACAGCCTATTCAAAAGGTGCGTTTACGGCGAAAGATC
AAACGAAATTGGCTCAGGTGTTTGCTGAATTAGCAAGTCGAGGCGTACAGGTGATGCTGTCTAACTCAGATTGCTCGTTT
ATCCGCGACCTTTACCAGTCGTTCAACATTTACTCGATTCAAGCGGCTCGGGCCATCAACTCTAACCCCCAAAAGCGCGG
CAAGATTACGGAGGTATTGGTCACATCCTGGTAA

Upstream 100 bases:

>100_bases
CCCATAGTGATTTAGTCGATATATATTATTGATTTCTCTGATTCACAGGATTGATCTTAACCCAAAGCCTTTACCTTCTA
CATTGTGGGGTTGCCCGTCC

Downstream 100 bases:

>100_bases
TCATCGTGGGTTGAGTGAACCTCTCAGGATTCTCATTAATCCCTTATGGTCTGTTCAGCCAATTACAGGGACTCACCCAG
ATCGCCATCATTCCAGCTTC

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.LlaDCHI A; M.LlaDCHIA; Adenine-specific methyltransferase LlaDCHIA; M.LlaII A [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTSPLPTQARVPARPFLKWAGGKGRLITQYGPHFPQNFGAYYEPFLGGGAIFFHLQPEQAVLSDVNPELVNVYTCVRDRI
DDVLKYLETHACQHGHDYYYQIRALNPSLPAERAARFIYLNRTCFNGLYRENSKGQFNVPMGRYKKPKICDVVLLKAVSE
VLQAVQIHQQPFETILEVAQSAQDFVYFDPPYHPISATSDFTAYSKGAFTAKDQTKLAQVFAELASRGVQVMLSNSDCSF
IRDLYQSFNIYSIQAARAINSNPQKRGKITEVLVTSW

Sequences:

>Translated_277_residues
MTSPLPTQARVPARPFLKWAGGKGRLITQYGPHFPQNFGAYYEPFLGGGAIFFHLQPEQAVLSDVNPELVNVYTCVRDRI
DDVLKYLETHACQHGHDYYYQIRALNPSLPAERAARFIYLNRTCFNGLYRENSKGQFNVPMGRYKKPKICDVVLLKAVSE
VLQAVQIHQQPFETILEVAQSAQDFVYFDPPYHPISATSDFTAYSKGAFTAKDQTKLAQVFAELASRGVQVMLSNSDCSF
IRDLYQSFNIYSIQAARAINSNPQKRGKITEVLVTSW
>Mature_276_residues
TSPLPTQARVPARPFLKWAGGKGRLITQYGPHFPQNFGAYYEPFLGGGAIFFHLQPEQAVLSDVNPELVNVYTCVRDRID
DVLKYLETHACQHGHDYYYQIRALNPSLPAERAARFIYLNRTCFNGLYRENSKGQFNVPMGRYKKPKICDVVLLKAVSEV
LQAVQIHQQPFETILEVAQSAQDFVYFDPPYHPISATSDFTAYSKGAFTAKDQTKLAQVFAELASRGVQVMLSNSDCSFI
RDLYQSFNIYSIQAARAINSNPQKRGKITEVLVTSW

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:
GO:0003676: DNA adenine methylase
GO:0008168: DNA adenine methylase
GO:0009007: DNA adenine methylase
GO:0032259: DNA adenine methylase
GO:0032775: DNA adenine methylase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=267, Percent_Identity=36.7041198501873, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31347; Mature: 31216

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPLPTQARVPARPFLKWAGGKGRLITQYGPHFPQNFGAYYEPFLGGGAIFFHLQPEQA
CCCCCCCCCCCCCCHHHEECCCCCCEEECCCCCCCCCCCCHHHHHCCCCEEEEEECCHHH
VLSDVNPELVNVYTCVRDRIDDVLKYLETHACQHGHDYYYQIRALNPSLPAERAARFIYL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHEEEEE
NRTCFNGLYRENSKGQFNVPMGRYKKPKICDVVLLKAVSEVLQAVQIHQQPFETILEVAQ
EHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAQDFVYFDPPYHPISATSDFTAYSKGAFTAKDQTKLAQVFAELASRGVQVMLSNSDCSF
CCCCEEEECCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHH
IRDLYQSFNIYSIQAARAINSNPQKRGKITEVLVTSW
HHHHHHHCCEEEEHHHHHHCCCCHHCCCEEHEEEECC
>Mature Secondary Structure 
TSPLPTQARVPARPFLKWAGGKGRLITQYGPHFPQNFGAYYEPFLGGGAIFFHLQPEQA
CCCCCCCCCCCCCHHHEECCCCCCEEECCCCCCCCCCCCHHHHHCCCCEEEEEECCHHH
VLSDVNPELVNVYTCVRDRIDDVLKYLETHACQHGHDYYYQIRALNPSLPAERAARFIYL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHEEEEE
NRTCFNGLYRENSKGQFNVPMGRYKKPKICDVVLLKAVSEVLQAVQIHQQPFETILEVAQ
EHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAQDFVYFDPPYHPISATSDFTAYSKGAFTAKDQTKLAQVFAELASRGVQVMLSNSDCSF
CCCCEEEECCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHH
IRDLYQSFNIYSIQAARAINSNPQKRGKITEVLVTSW
HHHHHHHCCEEEEHHHHHHCCCCHHCCCEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939; 11467810 [H]