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The map label for this gene is truB

Identifier: 158336142

GI number: 158336142

Start: 3034647

End: 3035516

Strand: Direct

Name: truB

Synonym: AM1_3004

Alternate gene names: 158336142

Gene position: 3034647-3035516 (Clockwise)

Preceding gene: 158336141

Following gene: 158336143

Centisome position: 46.66

GC content: 52.3

Gene sequence:

>870_bases
ATGCTTGGTTTTTTGAATTTGCATAAGCCTACTGGCATGACCTCTCATGATTGTGTCGGCAAAGTTCGGCGAATCTGTGG
CCTCAAGCGGGTGGGTCATGGGGGCACCCTGGATCCCTTGGCGACTGGCGTCTTGCCCATTGCCTTGGGACCGGCAACCC
GCCTCCTACACTATTTACCAGACCAAAAAACCTACCAAGCGACCATTCAGTTTGGCTTGACGACTACCACGGATGATTTA
GCTGGAGACATTCTGTCAGAACAGTCGGCTAACCATCTCGCTCAAGAGGTAGTTGAAAAAGCGCTACCGCAATTCCTCGG
TTCCATTGAACAACGCCCACCGGCCTATAGTGCAATCCAGGTTAAAGGTCAGCGCTTATACGATTTGGCTCGCCAAGGCA
AAGACGTAACGGCTCCCCTACGCCAAGTGGAGGTGTTCGACATTCAGATCTTGGACTGGCAGGGGGGAGAAAAATCGGAG
TTGCAGGTTGAGATCGCATGTGGTCCTGGCACTTATATTCGCTCTATCGCCAGGGATTTAGGTGAGGTTTTGGGGACTGG
GGCCACCTTAGCTCAACTCACGCGCACCCTAAGTTGTGGCTTTGCCCTCGCCGATAGTTTGACCTTTGAAACATTAGCGG
AGCAGGTTCAAGCCGGAACATTTTCACCCCTGGAGCCATCTTTTTCCCTACAACAACCGATTCTTCATTTGCCTCCGGCA
GAGGCCCAGCGTTTTTGCTGGGGGCAAAAGCTTCCTCAAGATTGTGCAGATGGCATGATGCAGGTGCATGACACTAGCGA
TCGCTTTTTAGGGATGGGGGAAATCATCGACGGGCTGCTCAAACCCAAGGTCGTATTGCCCGCACAATAA

Upstream 100 bases:

>100_bases
CTCCTCTAGCTATGCCGCCAATCTCAGTCGAGAACCCTTAACCTTGCATCTCACCCCCACTCTGACCTTCCCTGCAGCTC
AAACCTCGCAGGACTCTTGA

Downstream 100 bases:

>100_bases
AAATTGTGCCGATGACACCACTCCTAAAATCAGCAATGCTATACTCTGAGCCAAAATTATCGTTTCCTCCTCTCCAAACC
TCACTATGCGCCGGTTCACG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLGFLNLHKPTGMTSHDCVGKVRRICGLKRVGHGGTLDPLATGVLPIALGPATRLLHYLPDQKTYQATIQFGLTTTTDDL
AGDILSEQSANHLAQEVVEKALPQFLGSIEQRPPAYSAIQVKGQRLYDLARQGKDVTAPLRQVEVFDIQILDWQGGEKSE
LQVEIACGPGTYIRSIARDLGEVLGTGATLAQLTRTLSCGFALADSLTFETLAEQVQAGTFSPLEPSFSLQQPILHLPPA
EAQRFCWGQKLPQDCADGMMQVHDTSDRFLGMGEIIDGLLKPKVVLPAQ

Sequences:

>Translated_289_residues
MLGFLNLHKPTGMTSHDCVGKVRRICGLKRVGHGGTLDPLATGVLPIALGPATRLLHYLPDQKTYQATIQFGLTTTTDDL
AGDILSEQSANHLAQEVVEKALPQFLGSIEQRPPAYSAIQVKGQRLYDLARQGKDVTAPLRQVEVFDIQILDWQGGEKSE
LQVEIACGPGTYIRSIARDLGEVLGTGATLAQLTRTLSCGFALADSLTFETLAEQVQAGTFSPLEPSFSLQQPILHLPPA
EAQRFCWGQKLPQDCADGMMQVHDTSDRFLGMGEIIDGLLKPKVVLPAQ
>Mature_289_residues
MLGFLNLHKPTGMTSHDCVGKVRRICGLKRVGHGGTLDPLATGVLPIALGPATRLLHYLPDQKTYQATIQFGLTTTTDDL
AGDILSEQSANHLAQEVVEKALPQFLGSIEQRPPAYSAIQVKGQRLYDLARQGKDVTAPLRQVEVFDIQILDWQGGEKSE
LQVEIACGPGTYIRSIARDLGEVLGTGATLAQLTRTLSCGFALADSLTFETLAEQVQAGTFSPLEPSFSLQQPILHLPPA
EAQRFCWGQKLPQDCADGMMQVHDTSDRFLGMGEIIDGLLKPKVVLPAQ

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=256, Percent_Identity=32.03125, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI2367200, Length=298, Percent_Identity=37.9194630872483, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17553978, Length=196, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324037, Length=135, Percent_Identity=39.2592592592593, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=208, Percent_Identity=28.3653846153846, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364189, Length=198, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364187, Length=198, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364185, Length=198, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364183, Length=198, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62471759, Length=198, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17975520, Length=198, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_ACAM1 (B0CCH1)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517316.1
- ProteinModelPortal:   B0CCH1
- SMR:   B0CCH1
- GeneID:   5681812
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3004
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   PRK02755
- BioCyc:   AMAR329726:AM1_3004-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 31311; Mature: 31311

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGFLNLHKPTGMTSHDCVGKVRRICGLKRVGHGGTLDPLATGVLPIALGPATRLLHYLP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCHHHHHHHHCC
DQKTYQATIQFGLTTTTDDLAGDILSEQSANHLAQEVVEKALPQFLGSIEQRPPAYSAIQ
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEE
VKGQRLYDLARQGKDVTAPLRQVEVFDIQILDWQGGEKSELQVEIACGPGTYIRSIARDL
ECHHHHHHHHHCCCCCCCHHHHEEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHH
GEVLGTGATLAQLTRTLSCGFALADSLTFETLAEQVQAGTFSPLEPSFSLQQPILHLPPA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
EAQRFCWGQKLPQDCADGMMQVHDTSDRFLGMGEIIDGLLKPKVVLPAQ
HHHHHHHHCCCCHHHHHHHEECCCCCHHHEEHHHHHHHHHCCCEECCCC
>Mature Secondary Structure
MLGFLNLHKPTGMTSHDCVGKVRRICGLKRVGHGGTLDPLATGVLPIALGPATRLLHYLP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCHHHHHHHHCC
DQKTYQATIQFGLTTTTDDLAGDILSEQSANHLAQEVVEKALPQFLGSIEQRPPAYSAIQ
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEE
VKGQRLYDLARQGKDVTAPLRQVEVFDIQILDWQGGEKSELQVEIACGPGTYIRSIARDL
ECHHHHHHHHHCCCCCCCHHHHEEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHH
GEVLGTGATLAQLTRTLSCGFALADSLTFETLAEQVQAGTFSPLEPSFSLQQPILHLPPA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
EAQRFCWGQKLPQDCADGMMQVHDTSDRFLGMGEIIDGLLKPKVVLPAQ
HHHHHHHHCCCCHHHHHHHEECCCCCHHHEEHHHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA